• Title/Summary/Keyword: Component development

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Disinfection of Fusarium-infected Rice Seeds by Prochloraz and Gaseous Chlorine Dioxide

  • Jeon, Young-ah;Lee, Young-yi;Lee, Ho-sun;Sung, Jung-sook;Lee, Seokyoung
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.25-25
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    • 2014
  • Three species of Fusarium, F. fujikuroi, F. verticillioides and F. proliferatum, are known to be associated with bakanae disease of rice [1, 2]. F. fujikuroi infects rice flowers and survive in endosperm and embryo of the seeds. Infected seed is an important source of primary inoculum of pathogens [3]. Seeds of rice (Oryza sativa cv. Boramchan) collected from bakanae-infected field were found to be 96% infected with Fusarium sp., 52% with F. fujikuroi, 42% with F. verticillioides, and 12% with F. proliferatum as determined by incubation method and species-specific PCR assays. F. fujikuroi was detected at lemma/palea, endosperm and embryo whereas F. verticillioides and F. proliferatum were recovered only from lemma/palea by means of component plating test. Seed disinfection methods have been developed to control bakanae disease and prochloraz has been most widely used for rice seeds. Two chemicals formulated with prochloraz (PC 1) and prochloraz + hexaconazole (PC 2) that inhibit biosynthesis of ergosterol strongly reduced the incidence of Fusarium spp. on selective media to 4.7% and 2.0%, respectively. Disease symptoms of rice seedlings in nursery soil were alleviated by chemical treatment; seedlings with elongated leaves or wide angle between leaf and stem were strikingly reduced from 15.6 to 3.2% (PC 1) and 0 (PC 2), stem rots were reduced from 56.9 to 26.2% (PC 1) and 32.1% (PC 2), and normal seedling increased from 0.4 to 13.3% (PC 2). Prochloraz has some disadvantages and risks such as the occurrence of tolerant pathogens [4] and effects on the sterol synthesis in animals and humans [5]. For these reasons, it is necessary to develop new disinfection method that do not induce fungal tolerance and are safe to humans and animals. Chlorine dioxide ($ClO_2$), that is less toxic, produces no harmful byproducts, and has high oxidizing power, has been reported to be effective at disinfection of several phytopathogenic fungi including Colletotrichum spp. and Alternaria spp. [6]. Gaseous $ClO_2$ applied to rice seeds at a concentration of 20 ppm strongly suppressed mycelial growth of Fusarium fujikuroi, F. verticillioides and F. proliferatum. The incidence of Fusarium spp. in dry seed with 8.7% seed moisture content (SMC) tended to decrease as the concentration of $ClO_2$ increased from 20 to 40 ppm. Applying 40 ppm $ClO_2$ at 90% relative humidity, incidence was reduced to 5.3% and resulted in significant reduction of disease symptoms on MS media. In nursery soil, stem rot was reduced from 56.9 to 15.4% and the number of normal seedlings increased from 0.4 to 25.5%. With water-soaked seeds (33.1% SMC) holding moisture in the endosperm and embryo, the effectiveness of disinfection using $ClO_2$ increased, even when treated with only 20 ppm for four hours. This suggests that moisture was a key element for action of $ClO_2$. Removal of the palea and lemma from seeds significantly decreased the incidence of Fusarium spp. to 3.0%. Seed germination appeared to decrease slightly by water-soaking at $30^{\circ}C$ because of increased SMC and by physical damage of embryos from hulling. These results indicate that the use of gaseous $ClO_2$ was effective as a means to disinfect rice seeds infected with Fusarium spp. and that moisture around the pathogens in the seed was an important factor for the action of $ClO_2$. Further investigations should be conducted to ascertain the best conditions for complete disinfection of Fusarium spp. that infect deep site of rice seeds.

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An Evaluation of NURI(New University for Regional Innovation): Focusing on Changes in Graduate Employment (졸업생 취업률 변화를 중심으로 본 지방대학혁신역량강화(NURI)사업의 평가)

  • Lee, Sam-Ho;Kim, Hisam
    • KDI Journal of Economic Policy
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    • v.30 no.2
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    • pp.157-183
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    • 2008
  • 'New Universities for Regional Innovation(NURI)' is a financial aid program designed to promote the development of universities as a major component of Regional Innovation System (RIS). In particular, this program emphasizes the role of regional universities to provide the qualified graduates for the regional economy. This paper is to evaluate the effect of NURI, focusing on the change of graduates' employment. The effect of the program can be evaluated by the quality of graduates' accumulated human capital, and graduates' employment performance represents the graduates' quality evaluated in the labor market. This is also believed to be a good performance indicator of the NURI program. We utilize the graduate employment survey of Korean Educational Development Institute (KEDI), and calculate the graduates' employment rates of the departments that received the financial support of NURI (treatment group). We also calculate the graduates' employment rates of the departments that applied for the support of the NURI program but were not selected (comparison group). By using difference-in-differences method, we compare the change of graduates' employment rates in treatment and comparison groups before and after the program came in effect. Compared with the employment rates in 2004 before the NURI program started, the graduates employment rates improved in both groups in 2005 and 2006. The improvement of the employment rates in the treatment group is larger than that in the comparison group. Moreover, the difference of improvement gets larger in the year 2006 than in 2005, which means those students who were affected more years by the NURI program are more likely to be employed. However, the difference is not statistically significant, and we cannot definetely conclude that NURI showed the desired effect on the quality of the college graduates. We calculate employment rates in two ways; whether to treat going on to graduate education as an employment or not. The result was qualitatively the same in both cases. We also tracked quality of employment by investigating the firm size where the graduates of the treatment group were employed. By utilizing data from the Employment Insurance Fund, we measure the firm size by the number of employees. We did not find any deterioration of employment quality between 2005 and 2006, though it deteriorates in 2007. Therefore, the improvement of employment rates until 2006, though not statistically significant, does not seem to come at the cost of employment quality. The interpretation of this result cannot help being very limited. First, this evaluation covers such a short time period. It only covers two years after the program started, 2005 and 2006. Second, the extent of the improvement in employment rates is not satisfactory considering the amount of financial support, even though it can be argued that the employment has improved since the inception of the program. Subsequent evaluation of the program is required to certify the NURI programs' longer term effectiveness.

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Quantifying Uncertainty of Vitamin C Determination in Infant Formula by Indophenol Titration Method (인도페놀 적정법에 의한 성장기용조제식 중 비타민 C 함량분석의 측정불확도 산정)

  • Jun, Jang-Young;Kwak, Byung-Man;Ahn, Jang-Hyuk;Kong, Un-Young
    • Korean Journal of Food Science and Technology
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    • v.37 no.3
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    • pp.352-359
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    • 2005
  • Uncertainty involved during determination of vitamin C content in infant formula was quantified by indophenol titration method. Uncertainty sources in measurand, such as purity, weight, final volume of standard, volume of standard solution used for titration, sample weight, final volume of sample, extraction solution used for titration, titration of extraction solution and standard solution by indophenol solution were identified and used as parameters for combined standard uncertainty based on Guide to the expression of uncertainty in measurement (GUM) and Draft EURACHEM/CITAC Guide. Uncertainty parameters of each source in measurand were identified as resolution, reproducibility and stability of chemical balance, standard material purity, repeatability, reproducibility, end point of titration, 1 mL pipet, 5 mL autopipet, and 100 mL mass flask. Each uncertainty component was evaluated by types A and B and included to calculate combined uncertainty. Analytical test result for traceability under laboratorial conditions using Certified Reference Material (CRM) test was certified as $108.4{\pm}1.7mg/100g$, which was within CRM certification range of $114.6{\pm}6.6mg/100g$. Uncertainty test result of vitamin C content of 5 g sampling was $56.7{\pm}2.44mg/100g$. Uncertainty could be reduced by identification of uncertainty sources and components related with vitamin C determination by indophenol titration method and by decreasing uncertainty sources and components.

Estimation of Rice and Soybean Growth Stage Using a Microwave Scatterometer (마이크로파 산란계를 이용한 벼, 콩 생육단계 추정)

  • Kim, Yi-Hyun;Hong, Suk-Young;Lee, Hoon-Yol;Lee, Jae-Eun;Lee, Kyung-Do
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.503-510
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    • 2012
  • Microwave radar can penetrate cloud cover regardless of weather conditions and can be used day and night. Especially a A ground-based polarimetric scatterometer operating at multiple frequencies can continuously monitor the crop conditions. We analyzed scattering characteristics of rice and soybean using pauli decomposition method. Surface scattering (${\alpha}$) is the dominant component over the entire stages for all bands and pauli decomposition value was the highest for L-band. Double bounce scattering (${\beta}$) and volume scattering (${\gamma}$) were approximately equal for C-band and volume scattering was higher than double bounce scattering for X-band in rice field. In soybean, double bounce scattering becomes higher than volume scattering during the R2 stage (DOY 224) and there was a significant difference between the two components after the R4 stage (DOY 242) for L-band. The maximum growth stage of soybean can also be detected using L-band double bounce scattering. The peak of double bounce effect coincides with the peak of growth biophysical variables on DOY 271. We found that pauli decomposition can provide insight on the relative magnitude of different scattering mechanisms during the rice and soybean growth cycle.

Seasonal and Spatial Distribution of Soft-bottom Polychaetesin Jinju Bay of the Southern Coast of Korea (진주만에서 저서 다모류의 시 · 공간 분포)

  • Kang Chang Keun;Baik Myung Sun;Kim Jeong Bae;Lee Pil Yong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.1
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    • pp.35-45
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    • 2002
  • Seasonal quantitative van Veen grab sampling was conducted to characterize the composition and structure of the benthic polychaete community inhabiting the shellfish farming ground of a coastal bay system of Jiniu Bay (Korea). A total of 132 polychaete species were identified and the polychaetes accounted for about $80\%$ of overall abundance of benthic animals. There was little significant seasonal difference in densities (abundances) of polychaetes, Maximum biomass was obseued in summer (August) and minimum value was recorded in winter (February) and spring (May). Conversely, diversity and richness were lowest in summer, indicating a seasonal variability in the polychaetous community structure, The cluster analysis indicated that such a seasonal variability resulted mainly from the appearance of a few small, r-selected opportunists in spring and the tubiculous species of the family Maldanidae in summer. On the other hand, several indicator species for the organically enriched environments such as Capitelia capitata, Notoniashs Jatericeus and hmbrineris sp. showed high densities during all the study period. Density and biomass of univariate measures of community structure were significantly lower in the arkshell-farming ground of the southern area than in the non-farming sites of the bay, A similar general tendency was also found in the spatial distributions of species diversity and richness. Principal component analysis revealed the existence of different groups of benthic assemblages between the arkshell-farming ground and non-farming sites, The lack of colonization of r-selected opportunists and/or tubiculous species in the former ground seemed to contribute to the spatial differences in the composition and structure of the polychaetous communities. Although finer granulometric composition and high sulfide concentration in sediments of the arkshell-farming ground and low salinity in the northern area were likely to account for parts of the differences, other environmental variables observed were unlikely. The spatial distribution of polychaetes in Jiniu Bay may be rather closely related to the sedimentary disturbance by selection of shells for harvesting in spring.

A Study on the establishment of IoT management process in terms of business according to Paradigm Shift (패러다임 전환에 의한 기업 측면의 IoT 경영 프로세스 구축방안 연구)

  • Jeong, Min-Eui;Yu, Song-Jin
    • Journal of Intelligence and Information Systems
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    • v.21 no.2
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    • pp.151-171
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    • 2015
  • This study examined the concepts of the Internet of Things(IoT), the major issue and IoT trend in the domestic and international market. also reviewed the advent of IoT era which caused a 'Paradigm Shift'. This study proposed a solution for the appropriate corresponding strategy in terms of Enterprise. Global competition began in the IoT market. So, Businesses to be competitive and responsive, the government's efforts, as well as the efforts of companies themselves is needed. In particular, in order to cope with the dynamic environment appropriately, faster and more efficient strategy is required. In other words, proposed a management strategy that can respond the IoT competitive era on tipping point through the vision of paradigm shift. We forecasted and proposed the emergence of paradigm shift through a comparative analysis of past management paradigm and IoT management paradigm as follow; I) Knowledge & learning oriented management, II) Technology & innovation oriented management, III) Demand driven management, IV) Global collaboration management. The Knowledge & learning oriented management paradigm is expected to be a new management paradigm due to the development of IT technology development and information processing technology. In addition to the rapid development such as IT infrastructure and processing of data, storage, knowledge sharing and learning has become more important. Currently Hardware-oriented management paradigm will be changed to the software-oriented paradigm. In particular, the software and platform market is a key component of the IoT ecosystem, has been estimated to be led by Technology & innovation oriented management. In 2011, Gartner announced the concept of "Demand-Driven Value Networks(DDVN)", DDVN emphasizes value of the whole of the network. Therefore, Demand driven management paradigm is creating demand for advanced process, not the process corresponding to the demand simply. Global collaboration management paradigm create the value creation through the fusion between technology, between countries, between industries. In particular, cooperation between enterprises that has financial resources and brand power and venture companies with creative ideas and technical will generate positive synergies. Through this, The large enterprises and small companies that can be win-win environment would be built. Cope with the a paradigm shift and to establish a management strategy of Enterprise process, this study utilized the 'RTE cyclone model' which proposed by Gartner. RTE concept consists of three stages, Lead, Operate, Manage. The Lead stage is utilizing capital to strengthen the business competitiveness. This stages has the goal of linking to external stimuli strategy development, also Execute the business strategy of the company for capital and investment activities and environmental changes. Manege stage is to respond appropriately to threats and internalize the goals of the enterprise. Operate stage proceeds to action for increasing the efficiency of the services across the enterprise, also achieve the integration and simplification of the process, with real-time data capture. RTE(Real Time Enterprise) concept has the value for practical use with the management strategy. Appropriately applied in this study, we propose a 'IoT-RTE Cyclone model' which emphasizes the agility of the enterprise. In addition, based on the real-time monitoring, analysis, act through IT and IoT technology. 'IoT-RTE Cyclone model' that could integrate the business processes of the enterprise each sector and support the overall service. therefore the model be used as an effective response strategy for Enterprise. In particular, IoT-RTE Cyclone Model is to respond to external events, waste elements are removed according to the process is repeated. Therefore, it is possible to model the operation of the process more efficient and agile. This IoT-RTE Cyclone Model can be used as an effective response strategy of the enterprise in terms of IoT era of rapidly changing because it supports the overall service of the enterprise. When this model leverages a collaborative system among enterprises it expects breakthrough cost savings through competitiveness, global lead time, minimizing duplication.

Within Field Distribution Pattern and Design of a Sampling Plan for Damaged Onions by the Onion maggot, Hylemya antiqua Meigen(Diptera: Anthomyiidae) (고자리파리에 의한 양파피해(被害)의 포장내(圃場內) 분포양식(分布樣式)과 피해량(被害量) 추정(推定)을 위한 표본추출(標本抽出) 계획(計劃))

  • Park, C.G.;Hyun, J.S.;Cho, D.J.;Lee, K.S.;Hah, J.K.
    • Korean journal of applied entomology
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    • v.24 no.1 s.62
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    • pp.29-33
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    • 1985
  • Every plant in $990m^2$ onion field was inspected for damages by the onion maggot. Maps were constructed every ten days to show which plants were infested and which were not from April 11 to May 21, 1984. The maps were sectioned into squares one of which contains 80 onion plants and the counts of damaged onions in each square were fitted to poisson and negative binomial distribution and tested by chi-square. We argue that the satisfactory fitness of the expected negative binomial $[P(x^2)>0.05]$ provided a useful description of the spatial distribution patterns of the damaged onions. Edge effect was tested by the differences of damage ratio and variance/mean ratio (${\sigma}^2/m$) between edge and center part. The result showed that the damage ratioes and variances of all the periods, ${\sigma}^2/m$ values after May 1 were greater in edge part than in center part. Again, the maps were sectioned into four blocks and the squares (sample units) were sectioned into quadrants. By application of the variance component technique, it was suggested that $2{\sim}8$ sample units for 5% sampling error and $1{\sim}2$ sample units for 10% error should be sampled randomly to estimate the damage ratio when $2{\sim}3$ quadrants were inspected.

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Management of the Development of Insecticide Resistance by Sensible Use of Insecticide, Operational Methods (실행방식 측면에서 살충제의 신중한 사용에 의한 저항성 발달의 관리)

  • Chung, Bu-Keun;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.48 no.2
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    • pp.123-158
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    • 2009
  • An attempt was made to stimulate future research by providing exemplary information, which would integrate published knowledge to solve specific pest problem caused by resistance. This review was directed to find a way for delaying resistance development with consideration of chemical(s) nature, of mixture, rotation, or mosaics, and of insecticide(s) compatible with the biological agents in integrated pest management (IPM). The application frequency, related to the resistance development, was influenced by insecticide activity from potentiation, residual period, and the vulnerability to resistance development of chemical, with secondary pest. Chemical affected feeding, locomotion, flight, mating, and predator avoidance. Insecticides with negative cross-resistance by the difference of target sites and mode of action would be adapted to mixture, rotation and mosaic. Mixtures for delaying resistance depend on each component killing very high percentage of the insects, considering allele dominance, cross-resistance, and immigration and fitness disadvantage. Potential disadvantages associated with mixtures include disruption of biological control, resistance in secondary pests, selecting very resistant population, and extending cross-resistance range. The rotation would use insecticides in high and low doses, or with different metabolic mechanisms. Mosaic apply insecticides to the different sectors of a grid for highly mobile insects, spray unrelated insecticides to sedentary aphids in different areas, or mix plots of insecticide-treated and untreated rows. On the evolution of pest resistance, selectivity and resistance of parasitoids and predator decreased the number of generations in which pesticide treatment is required and they could be complementary to refuges from pesticides To enhance the viability of parasitoids, the terms on the insecticides selectivity and factors affecting to the selectivity in field were examined. For establishment of resistant parasitoid, migration, survivorship, refuge, alternative pesticides were considered. To use parasitoids under the pressure of pesticides, resistant or tolerant parasitoids were tested, collected, and/or selected. A parasitoid parasitized more successfully in the susceptible host than the resistant. Factors affecting to selective toxicity of predator are mixing mineral oil, application method, insecticide contaminated prey, trait of individual insecticide, sub-lethal doses, and the developmental stage of predators. To improve the predator/prey ratio in field, application time, method, and formulation of pesticide, reducing dose rate, using mulches and weeds, multicropping and managing of surroundings are suggested. Plant resistance, predator activity, selective insect growth regulator, and alternative prey positively contributed to the increase of the ratio. Using selective insecticides or insecticide resistant predator controlled its phytophagous prey mites, kept them below an economic level, increased yield, and reduced the spray number and fruits damaged.

A Comparative Study on Quantifying Uncertainty of Vitamin A Determination in Infant Formula by HPLC (HPLC에 의한 조제분유 중 비타민 A 함량 분석의 측정불확도 비교산정)

  • Lee, Hong-Min;Kwak, Byung-Man;Ahn, Jang-Hyuk;Jeon, Tae-Hong
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.152-159
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    • 2008
  • The purpose of this study was to determine the accurate quantification of vitamin A in infant formula by comparing two different standard stock solutions as well as various sample weights using high performance liquid chromatography. The sources of uncertainty in measurement, such as sample weight, final smaple vloume, and the instrumental results, were identified and used as parameters to determine the combined standard uncertainty based on GUM(guide to the expression of uncertainty in measurement) and the Draft EURACHEM/CITAC Guide. The uncertainty components in measuring were identified as standard weight, purity, molecular weight, dilution of the standard solution, calibration curve, recovery, reproducibility, sample weight, and final sample volume. Each uncertainty component was evaluated for type A and type B and included to calculate the combined uncertainty. The analytical results and combined standard uncertainties of vitamin A according to the two different methods of stock solution preparation were 627 ${\pm}$ 33 ${\mu}$g R.E./100 g for 1,000 mg/L of stock solution, and 627 ${\pm}$ 49 ${\mu}$g R.E./100 g for 100 mg/L of stock solution. The analytical results and combined standard uncertainties of vitamin A according to the various sample weighs were 622 ${\pm}$ 48 ${\mu}$g R.E./100 g, 627 ${\pm}$ 33 ${\mu}$g R.E./100 g, and 491 ${\pm}$ 23 ${\mu}$g R.E./100 g for 1 g, 2 g, and 5 g of sampling, respectively. These data indicate that the preparation method of standard stock solution and the smaple amount were main sources of uncertainty in the analysis results for vitamin A. Preparing 1,000 mg/L of stock solution for standard material sampling rather than 100 mg, and sampling not more than 2 g of infant formula, would be effective for reducing differences in the results as well as uncertainty.

Variation of leaf morphology among 18 populations of Zelkova serrata Mak. (느티나무 18개 집단의 엽 형질 변이)

  • Kim, In-Sik;Kwon, Hae-Yun;Ryu, Keun-Ok;Choi, Hyung-Soon
    • Korean Journal of Breeding Science
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    • v.42 no.1
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    • pp.40-49
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    • 2010
  • This study was conducted to examine genetic variation on leaf characteristics of Zelkova serrata populations. Leaf samples were obtained from eighteen populations and fourteen leaf characteristics such as leaf blade length, leaf width, leaf area and etc. were measured. In all leaf characteristics, there were significant differences among populations and among individuals within population. Most variance of leaf characteristics was contributed to among individuals within population except for length from leaf base to maximum width (x3) and the ratio of x3 to leaf blade length (x10). The relatively high variations of leaf characteristics were found at Gurye, Jungeup and Gyungju populations while Youngwol, Cheongsong, Youngchun populations showed the lower variation. There was high correlation among leaf characteristics related to leaf size, but not among the variables of ratio between leaf characteristics. Length from leaf base to maximum width(x3) and the ratio of x3 to x10 showed significant positive correlation with latitude and altitude of populations, which reflect the differences of mean annual temperature among populations. Mean annual rainfall of populations showed negative correlation with leaf blade length, leaf width, length from apex to first serration and leaf area. Four principal components (PC) were deduced from principal component analysis, which explain the 88.5% of total variance of leaf characteristics. Leaf area, length from leaf base to maximum width, serration number and petiole length showed the highest contribution to PC1, PC2, PC3, PC4, respectively. According to cluster analysis, the populations of Z. serrata were divided into two groups, which reflect the difference of mean annual temperature between groups. Within group, however, specific tendency of clustering was not observed among populattions.