• Title/Summary/Keyword: Component development

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Suppression subtractive hybridization (SSH) for isolation and characterization of genes related to testicular development in the giant tiger shrimp Penaeus monodon

  • Leelatanawit, Rungnapa;Klinbunga, Sirawut;Aoki, Takashi;Hirono, Ikuo;Valyasevi, Rudd;Menasveta, Piamsak
    • BMB Reports
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    • v.41 no.11
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    • pp.796-802
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    • 2008
  • Suppression subtractive hybridization (SSH) cDNA libraries of the giant tiger shrimp, Penaeus monodon, were constructed. In total, 178 and 187 clones from the forward and reverse SSH libraries, respectively, of P. monodon were unidirectionally sequenced. From these, 37.1% and 53.5% Expressed Sequence Tags (ESTs) significantly matched known genes (E-value < 1e-04). Three isoforms of P. monodon progestin membrane receptor component 1: PM-PGMRC1-s (1980 bp), PM-PGMRC1- m (2848 bp), and PM-PGMRC1-l (2971 bp), with an identical ORF of 573 bp corresponding to a deduced polypeptide of 190 amino acids, were successfully identified by RACE-PCR. Interestingly, PMPGMRC1 showed a greater expression level in testes of juvenile than broodstock P. monodon (P < 0.05). Dopamine administration ($10^{-6}$ mol/shrimp) resulted in up-regulation of PM-PGMRC1 in testes of juveniles at 3 hrs post treatment (P < 0.05), but had no effect on PM-Dmc1 (P > 0.05).

Structural Relationships among Rural Tourism Motivation, Satisfaction, and Loyalty Including the Moderating Effects of Gender (농촌관광에 있어서 관광동기와 만족이 충성도에 미치는 구조관계 분석: 성별의 조절효과)

  • Park, Duk-Byeong;Kim, Kyung-Hee
    • The Korean Journal of Community Living Science
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    • v.22 no.2
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    • pp.283-298
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    • 2011
  • Gender is frequently being identified as a key moderator in consumer behavior studies. However, there has been insufficient studies in this field in rural tourism areas. The purpose of this study examined the structural relationships among rural tourism motivation, satisfaction, and loyalty including the moderating effect of gender. Data were collected from 442 farmstay visitors by field survey in rural areas. SPSS 15.0 and LISREL 8.80 statistical package were used to analyze the data. Principle component analysis and path analysis were carried out to identify the structural relationships. Results showed that refreshment and family togetherness of tourism motivation had a negative effect on satisfaction. However, rural life of tourism motivation had a positive effect on satisfaction. Tourists' satisfaction had a positive effect on loyalty. The results also indicated that gender moderated the relationship among tourism motivation, satisfaction, and loyalty. The study suggested that it was important for tourism service marketers to develop proactive relationship marketing techniques that could have a lasting impact on customer retention.

Daughters' Mourning Experience from Loss of their Mothers (엄마를 상실한 중년여성의 애도 경험)

  • Choi, Seung-Yee
    • The Korean Journal of Community Living Science
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    • v.22 no.4
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    • pp.637-653
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    • 2011
  • Over the last 20 years, women's development has been ambiguous. According to Freud, the mother-daughter relationship has been considered as an essential component of women's development. This study investigated how the mourning experience from daughters who were separated from their mothers by death offers any meaningful idea to women's life. For Hermeneutic phenomenology, researchers collected data from three women who have lost their mothers through in-depth interviews and drawing pictures with each of them. As a result, this study found that daughters started to fight with their mothers' death from the moment when they were informed. They also experienced a symbolic death until the moment of their mothers' death and even after the confirmation of their mothers' death. Daughters experienced symbolic death, both emotionally and physically as well as familiar experiences with their mothers through the repeated confirmation of death. However, these experiences encouraged daughters to adapt to the reality that was the absence of their mothers through the processes such as "Reflection," "Living in the Center of My life," and "Another Embrace." Through this new perspective, attempts, and relationships, daughters form a new identity and experience 'rebirth'. These daughters' changes were paradoxically strengthened through their resistances towards changes of reformation of themselves.

Dynamic Model Development and Simulation of Crawler Type Excavator (크롤러형 굴삭기의 동역학적 모델 개발 및 시뮬레이션)

  • Kwon, Soon-Ki
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.18 no.6
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    • pp.642-651
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    • 2009
  • The history of excavator design is not long enough which still causes most of the design considerations to be focused on static analysis or simple functional improvement based on static analysis. However, the real forces experiencing on each component of excavator are highly transient and impulsive. Therefore, the prediction and the evaluation of the movement of the excavator by dynamic load in the early design stage through the dynamic transient analysis of the excavator and ensuring of design technique plays an importance role to reduce development-cost, shorten product-deliver, decrease vehicle-weight and optimize the system design. In this paper, Commercial software DADS and ANSYS help to develop the track model of the crawler type excavator, and to evaluate the performance and the dynamic characteristics of excavator with various simulations. For that reason, the track of crawler type excavator is modelled with DADS Track Vehicle Superelement, and the reaction forces on the track rollers were predicted through the driving simulation. Also, the upper frame and cabin vibration characteristics, at the low RPM idle state, were evaluated with engine rigid body modelling. And flexibility body effects were considered to determine the more accurate joint reaction forces and accelerations under the upper frame swing motion.

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A study of quality attributes for reliability improvement on traffic information (교통정보 신뢰성 향상을 위한 품질특성에 관한 연구)

  • Chung, Sung-Hak
    • Journal of the Korea Society of Computer and Information
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    • v.18 no.5
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    • pp.133-145
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    • 2013
  • The objective of this study is to contribute effective operation of traffic information center by traffic information data measure process which are now being serviced by frame work of traffic information and by providing traffic information assessment criteria which reliable traffic information throughout traffic information quality characteristics in intelligent transport system. For the objectives, we analyzed the information attribute of ontology technique for quality management of traffic information in system engineering database research area. Throughout the result of this study, SE database utilized the development of subsystems, the component and interface design for convenient and rapid information services, and diverse related reliable systems design and expected to be indicated in systematic database.

The Design of an Integrated Platform 4A DUOS for Mobile User Interface Development (모바일 유저인터페이스 개발을 위한 통합 플랫폼 4A DUOS 설계)

  • Kang, Sunmee
    • Journal of IKEEE
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    • v.17 no.2
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    • pp.89-95
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    • 2013
  • Diverse operating systems and developed platforms of mobile devices are implemented by vendors exist. The software has to be developed by respective environment in designing the user interface of software. For the solution of the inconvenience, this paper proposes an integrated platform 4A DUOS for developing user interface independently. The proposed platform can use web language not dependent on various operating systems, and offer online access to API and component templates to provide the convenience of the user interface design. Also, the server with on board designed user interface implements the service to the user. Therefore, the users can design the independent user interface on various mobile devices by using only one source.

Development and Implementation of e-Teaching Portfolio in International Understanding Education for Secondary Pre-service Teachers (중등 예비교사를 위한 국제이해교육 과목의 e-티칭 포트폴리오 개발 및 적용)

  • Lee, Eun-Hwa
    • The Journal of Korean Association of Computer Education
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    • v.14 no.5
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    • pp.55-69
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    • 2011
  • The purpose of this study is to develop and apply e-teaching portfolio in EIU for pre-service teachers, and to explore the possibility of applying e-teaching portfolio in pre-service teacher education. E-teaching portfolio has developed an online platform after designing the component of teaching portfolio. And then pre-service teachers' perceptions were investigated. The findings, pre-service teachers thought that e-teaching portfolio was useful for the accumulation and management of instructional materials, and gave opportunities to improve self-reflection, teaching skills and evidence over the professional development through communication.

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Increasing plant yield by modulating root hair development in Brachypodium distachyon

  • Kim, Chul Min
    • Korean Journal of Agricultural Science
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    • v.47 no.2
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    • pp.305-313
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    • 2020
  • Root hair development has the potential to increase crop yields and at the same time to decrease fertilizer use, which will be required in the next 30 years to meet the demand for crop-derived commodities in a world with decreasing available natural resources. Root hair defective six (RHD6) encoding a basic helix-loop-helix transcription factor, is associated with root hair differentiation, and its roles are root hair initiation and elongation. Grass plants, rice and Brachypodium have been used as model plants to study the gene function of the root hair defective six like (RSL) subfamily which is orthologous to AtRHD6. The RSL subfamily has an identical gene function with AtRHD6 which is involved with root hair differentiation as well. Plants with longer root hairs within a species should have an improved Pi uptake efficiency; therefore, we would expect that a plant with a high Pi uptake could contribute to increasing the plant yield. We achieved increased root hair length by manipulating the RSL subfamily genes. It is expected that in these transgenic plants, the long root hairs would be sufficient to improve the Pi uptake and hence improve biomass and yield component (tiller, spikelet number, and spikelet weight) of the plant. Here, we demonstrate that Brachypodium transgenic plants overexpressing the BdRSL subfamily genes have an improved biomass and grain yield. The result of this study could be applied to important crop plants like rice.

Recent Trends of Advanced Biosensors for Mycotoxin Analysis

  • Shim, Won-Bo
    • 한국균학회소식:학술대회논문집
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    • 2016.05a
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    • pp.35-35
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    • 2016
  • A mycotoxin is a toxic secondary metabolite produced by organisms of the fungus kingdom, commonly known as molds and has been widely contaminated in agricultural products such as grains and cereals. Many methods including high performance liquid chromatography (HPLC) and gas chromatography (GC) have already been proposed and reviewed for mycotoxins. These methods are either expensive or time-consuming due to the complication of sample preparation and pre-concentration before determination. In addition, both methods are unsuitable for the routine screening of large sample numbers. A biosensor is a fictive analytical device that combines a biological component with a physicochemical detector for the detection of an analyte. Biosensors represent a rapidly expanding field, at the present time, with an estimated 60% annual growth rate; the major impetus coming from the health-care industry but with some pressure from other areas, such as food safety and environmental monitoring. Antibodies and aptamers are bioreceptors which have been used in the development of biosensors. There are many kinds of antibodies and aptamers specific to mycotoxin, and antibody (or aptamer)-based biosensors have been successfully developed for the detection of mycotoxin. The biosensors permit the rapid, sensitive, simple, and on-site detection of a range of mycotoxins and can be an alternative method to traditional methods such as HPLC and GC. This presentation provides the development trends of biosensors to mycotoxins and their application to food and agricultural products.

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Efficient Cyber Lecture System using SCC (강의객체를 이용한 효율적인 가상강의 시스템)

  • 강정배;김선경
    • Journal of Korea Society of Industrial Information Systems
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    • v.9 no.1
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    • pp.49-55
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    • 2004
  • E-Learning standard SCORM has been prepared in the United States to establish the efficient operation and development of e-Learning. The learning object of the current SCORM focuses on easy development and reusability in the professor's side. In this study instead, we present a SCC (Sharable Content Collection) scheme which can provide various studying opportunities to the learners. Constructing an efficient SCC requires improving the SCO (Sharable Content Object), a key ingredient of SCORM, and analyzing the cyber lecture into components. In this way we suggest a method to improve the existing learning objects and present an e-Learning model based on SCC.

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