• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2671-2674
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• 2013

To elucidate the role of the evolutionarily conserved Tyr8 residue in rice Phi-class GSTF3, this amino acid was replaced with alanine and phenylalanine by site-directed mutagenesis, respectively. The replacement of Tyr8 with Ala significantly affected the catalytic activity and the kinetic parameters, whereas the substitutions of Tyr8 with Phe had almost no effect. The Y8A mutant resulted in approximately 90-100% decrease of the specific activity. Moreover, the Y8A mutant resulted approximately in 2-fold increase of $K_m$, approximately 60-80% decrease of $k_{cat}$, and approximately 6.5-fold decrease in $k_{cat}/K_m$. From the pH/log $k_{cat}/K_m$ plot, $pK_a$ values of the GSH in the wild-type enzyme-GSH complex, Y8A-GSH complex and Y8F-GSH complex were estimated to be approximately 6.8, 8.5 and 6.9, respectively. From these results, we suggest that the evolutionarily conserved Tyr8 residue in OsGSTF3 seems to influence the structural stability of the active site of OsGSTF3 rather than directly its catalytic activity.

• Journal of Korean Society for Atmospheric Environment
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• v.21 no.1
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• pp.83-96
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• 2005

The purposes of this study were to characterize the local levels of VOCs (volatile organic compounds), to develop source profiles of VOCs, and to quantify the source contribution of VOCs using the CMB (chemical mass balance) model. The concentration of VOCs had been measured every 6-day duration in the SRO monitoring site in the Yeosu Petrochemical Industrial Complex from September 2000 to August 2002. The total of 35 target VOCs, which were included in the TO-14 designated from the U.S. EPA, was selected to be monitored in the study area. During a 24-h period, the ambient VOCs were sampled by using canisters placing about 10 ～ 15 m above the ground level. The collected canisters were then analyzed by a GC-MS in the laboratory. Aside from ambient sampling at the SRO site, the VOCs had been intensively and massively measured from 8 direct sources and 4 general sources in the study area. The results obtained in the study were as follows; first, the annual mean concentrations of the target VOCs were widely distributed regardless of monitoring sites in the Yeosu Petrochemical Industrial Complex. In particular, the concentrations of BTX (Benzene, Toluene, Xylene), vinyl chloride were higher than other target compounds. Second, based on these source sample data, source profiles for VOCs were developed to apply a receptor model, the CMB model. Third, the results of source apportionment study for the VOCs in the SRO Site were as follows; The source of petrochemical plant was apportioned by 31.3% in terms of VOCs mass. The site was also affected by 16.7% from wastewater treatment plant, 14.0% from iron mills, 8.4% from refineries, 4.4% from oil storage, 3.8% from automobiles, 2.3% from fertilizer, 2.3% from painting, 2.2% from waste incinerator, 0.6% from graphic art, and 0.4% from gasoline vapor sources.

• KIEAE Journal
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• v.5 no.3
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• pp.11-16
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• 2005

The recent trends of high-density and high-rise in apartment housing have caused the problems of decrease in ventilation rates and increase of indoor pollutant contaminants. SHS(Sick House Syndrome) has now become a major issue and threats the health of residents. To solve these indoor air problems, increase in ventilation rate is considered as one of the most efficient approach. Thus, the recent housing development is pursuing improvement in the site design and the layout of apartment building blocks to promote natural ventilation is now investigated as one of the fundamental solutions. This study was focused on the air flow characteristics of outdoor environment in an apartment complex to keep the pollutants out of the site. Age of air and pressure difference have been used as indices of the outdoor air quality. Four different types of apartment building layouts have been analyzed by CFD simulation. This study again selected a real apartment housing complex as a case study model. By analyzing the pressure differences between the front and rear of an apartment building block, the ventilation performance in each individual unit was evaluated, and its impact on ventilation performance is investigated by analyzing the stagnant air around the apartment building blocks. During this process, existing patterns of apartment housing layout have been evaluated, and the most appropriate site layout has been chosen to analyze the outdoor airflow patterns. Based on the analysis of airflow patterns of site layout, the possibilities of improving ventilation performance of an individual apartment housing is proposed.

• Journal of Korean Society for Atmospheric Environment
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• v.19 no.6
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• pp.663-677
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• 2003

The concentrations of volatile organic compounds (VOCs) in the ambient air were measured at three sites (Samil-dong, SRO and EFMC) in Yeosu industrial complex during September 2000 to August 2001. Air samples were collected for 24 hours in Silicocan canister (6l) with constant flow samplers every 6 days and analyzed using a cryogenic preconcentration system and a GC/MS. At each site, we identified 35 species known as on both the carcinogenic and mutagenicity by the EPA US (TO-14 manual). No relationship was found between YOCs concentration at three sampling points. Furthermore, the result shows that there appears to be a variety distribution of the concentration. BTX, vinyl chloride and high concentrations of 1,2-dichloroethane were observed at the sampling sites. Especially, high concentration of toxic VOCs, such as vinyl chloride, chloroform, 1,2-dichloroethane and benzene were shown at environmental facilities management cooperation (EMFC) site. They seem to be emitted from the facility of wastewater treatment in Yeosu industrial complex. It was difficult to tell the seasonal variation of total VOCs concentration. Nevertheless, the concentration in winter was typically higher than in summer The concentrations of toxic VOCs contents in Yeosu industrial complex were generally lower than those in Ulsan complex, although those were similar or less than in Seoul and Daegu. Whereas, toluene and styrene emitted from Yeosu industrial complex were higher than those of Edmonton industrial complex in Canada. Especially, toluene was third times higher than those observed from Texas, USA.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.62-62
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• 2003

The native form of serpin (serine protease inhibitor) is kinetically trapped in metastable state. Metastability in these proteins is critical to their biological function. Serpins inhibit target proteases by forming a stable covalent complex in which the cleaved reactive site loop of the serpin is inserted into $\beta$-sheet A of the serpin with concomitant translocation of the protease to the opposite of the initial binding site. Despite recent determination of the crystal structures of a Michaelis protease-serpin complex as well as a stable covalent complex, details on the kinetic mechanism remain unsolved. In this study we constructed several $\alpha$$_1$-antitrypsin variants and examined their kinetic mechanism of loop translocation and formation of protease-serpin complex by stopped-flow experiments of fluorescence resonance energy transfer as well as quenched-flow experiment. We report here the relationship of serpin's conformational switch mechanism with Inhibitory activity. There is little direct correlation between loop insertion rate and inhibitory activity. Rather, disrupting a salt bridge between R196 and E354 accelerates loop translocation even though it impairs the inhibitory activity. Moreover, the serpin's reactive site loop is translocated, at least partially, prior to loop cleavage.

• The Korean Journal of Ecology
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• v.15 no.3
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• pp.231-246
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• 1992

In order to estimate effects of air pollution for pinus thunbergii forests arounding onsan industrial complex, structure of forest vegrtation was surveyed. In the survey of forest vegetation, pinus densiflora have been died or being withered, and importance value(I.V) of rhododendron mucromulatum and lespedeza spp. were low at the vicinity of industrial complex. Especially lespedeza maximowiczii and l. maritima were not entirely appeared at the vicinity of industrial complex. I.V. of quercus serrata, robinia pseudoacacia and alnus firma were preferably high at the vicinity of industrial complex, and those of smilax china, rubus crataegifolius and rhus trichocarpa were similar at every site and it could be guessed that those had a tolerance. Number of species had a difference over 10 species at the vicinity of industrial complex as compared with those of remote sites, and species diversity showed highly at the farther sites of industrial complex, and also at the low layer, number of species, species diversity and evennes were low at the vicinity of industrialcomplex and species weakened to air pollution were disappearing. It was possible that forest vegetation would be changed from pinus spp. to community of a. firma and r. pseudoacacia.

• Nuclear Engineering and Technology
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• v.50 no.8
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• pp.1210-1216
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• 2018

Site or multi-unit (MU) risk assessment has been a major issue in the field of nuclear safety study since the Fukushima accident in 2011. There have been few methods or experiences for MU risk assessment because the Fukushima accident was the first real MU accident and before the accident, there was little expectation of the possibility that an MU accident will occur. In addition to the lack of experience of MU risk assessment, since an MU nuclear power plant site is usually very complex to analyze as a whole, it was considered that a systematic method such as probabilistic safety assessment (PSA) is difficult to apply to MU risk assessment. This paper proposes a new MU risk assessment methodology by using the conventional PSA methodology which is widely used in nuclear power plant risk assessment. The logical failure structure of a site with multiple units is suggested from the definition of site risk, and a decomposition method is applied to identify specific MU failure scenarios.

• Journal of architectural history
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• v.25 no.1
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• pp.7-21
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• 2016

The purpose of this paper is to study on the characteristic of site planning of Hadrian's villa. The whole site planning of Hadrian's villa can be divided by different characteristic four groups(I-IV). It is analysed to the whole site plan of such huge complex and the site planning of partial each group which is organically connected each other. The results of this study are as follows: 1. Hadrian's villa was arranged in adapt to natural condition according to a gentle inclination by topography and terrain of the whole site which many different buildings are oriented towards natural landscape of the site and the site surroundings. 2. The site planning of Hadrian's villa is composed of dividing into four groups as plan segmentalized through various land use plan, considering for territory of functional aspect and direction of axis formed by situation and direction of topography and terrain. 3. The concept of site planning to buildings of Hadrian's villa is composed through each other connection; the first, meeting, crossing and merging with each other from arrangement by several axises, the second, change of direction with as the center a part of corner of buildings and the third, facing or inserting system to side and side, side and a part of corner of building.

• New & Renewable Energy
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• v.19 no.2
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• pp.40-58
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• 2023

Several offshore wind power conflicts occur due to the problems in which the site selection process led by private operators is improperly managed. To review the institutional improvement measures that solve this problem, domestic and foreign institutions and operational cases were comparatively analyzed, focusing on key actors from the multi-level governance perspective. First, the status of the site selection process in the Republic of Korea, major issues in stakeholder conflicts, and discussions on the planned site system-related laws (draft) were reviewed. Next, the site selection process and relevant cases in Germany, the Netherlands, and Japan were analyzed. In all these countries, site selection is done by the central government. In Germany and the Netherlands, maritime-related ministries establish overall offshore wind power site plans and conduct strategic environmental assessments for these plans. Futhermore, in the process of determining each individual site, extensive site investigation including environmental assessments are conducted. This aspect needs to be supplemented in the discussion on the direction of institutional improvement in the Republic of Korea.

• Journal of Environmental Impact Assessment
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• v.21 no.3
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• pp.417-429
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• 2012

In this study, a diagnostic wind model, CALMET and a micrometeorological numerical model, ENVI-MET were used to analyze the wind field in and out of the site designated for the industrial complex around Buron-myeon, Wonju, Gangwon-do. The results of modeling with CALMET showed that the air flow in industrial complex was little affected by the surrounding terrain. And the result of wind field analysis with ENVI-MET showed there are turbulent air flows such as cavity and wake around structures in the industrial complex, which can cause high-air pollution. Therefore, it is necessary to design the industrial complex considering the wind path according to wind directions.