• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1180-1184
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• 2006

The capsule that surrounds Yersinia pestis cells is composed of a protein-polysacchride complex; the purified protein component is fraction I (F1) antigen. We report the cloning of the cafl gene and its expression in Escherichia coli using the vector pETl02/D-TOPO and the F1-specific monoclonal antibody. The recombinant F1 (rF1) antigen had a molecular size of 17.5 kDa, which was identical to that of the F1 antigen produced by Y. pestis. Recombinant F1 protein was found to react to polyclonal antiserum to Y. pestis Fl. Recombinant F1 was purified by ProBond purification system and induced a protective immune response in BALB/c mice challenged with up to 10$^5$ virulent Y. pestis. Purified rF1 protein was used in an ELISA to evaluate the ability of a method to detect antibodies to Y. pestis in animal sera. These results strongly indicated that the rF1 protein is a suitable species-specific immunodiagnostic antigen and vaccine candidate.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1176-1184
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• 2013

Anti-hepatocyte growth factor (anti-HGF) monoclonal antibodies (mAbs) are potential therapeutics against various cancers. Screening for high-producer clones is a time-consuming and complex process and is a major hurdle in the development of therapeutic mAbs. Here, we describe an efficient approach that allows the selection of high-producer Chinese hamster ovary (CHO) cell lines producing the novel anti-HGF mAb SFN68, which was generated previously by immunizing HGF bound to its receptor c-Met. We selected an SFN68-producing parental cell line via transfection of the dihydrofolate reductase-deficient CHO cell line DG44, which was preadapted to serum-free suspension culture, with an SFN68-expression vector. Subsequent gene amplification via multiple passages of the parental cell line in a methotrexate-containing medium over 4 weeks, followed by clonal isolation, enabled us to isolate two cell lines, 2F7 and 2H4, with 3-fold higher specific productivity. We also screened 72 different media formulated with diverse feed and basal media to develop a suboptimized medium. In the established suboptimized medium, the highest anti-HGF mAb yields of the 2F7 and 2H4 clones were 842 and 861 mg/l, respectively, which were about 10.5-fold higher than that of the parental cell line in a non-optimized basal medium. The selected CHO cell lines secreting high titers of SFN68 would be useful for the production of sufficient amounts of antibodies for efficacy evaluation in preclinical and early clinical studies.

• Journal of Korean Tunnelling and Underground Space Association
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• v.8 no.2
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• pp.115-128
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• 2006

The structural anisotropy and heterogeneity of rock mass, caused by discontinuities and weak zones, have a great influence on the deformation behavior of a tunnel. A tunnel construction in these complex ground conditions is very difficult. No matter how excellent a geological investigation is, local uncertainties of rock mass conditions still remain. Under these uncertain circumstances, an accurate forecast of the ground conditions ahead of the advancing tunnel face is indispensable to a safe and economic tunnel construction. This paper presents the effect of anisotropy and heterogeneity of the rock masses to be excavated by numerical analysis. The influences of distance from weak zone, the size or dimension, the different stiffness and the orientation of weak zones are analysed by 3-D finite element analysis. By analysing these numerical results, the tunnel behavior due to excavation can be well understood and the prediction of rock mass condition ahead of the tunnel face can be possible.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.6
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• pp.283-292
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• 2015

A model of apartment heating load in a district heating system could be useful in the management and utilization of energy resources, since it could predict energy usage and so could assist in the efficient use of energy resources. The heating load in a district heating system varies in a highly nonlinear manner and is subject to many different factors, such as heating area, number of people living in that complex, and ambient temperature. Thus there are few published papers with accurate models of heating load, especially in domestic literature. This work is concerned with the modeling of apartment heating load in a district heating system in winter, using the reduced least square support vector machine (LS-SVM), and with the purpose of using the model to predict heating energy usage in domestic city area. We collected 23,856 pieces of data on heating energy usage over a 12-week period in winter, from 12 heat exchangers in five apartments. Half of the collected data were used to construct the heating load model, and the other half were used to test the model's accuracy. The model was able to predict the heating energy usage pattern rather accurately. It could also estimate the usage of heating energy within of mean absolute percentage error. This implies that the model prediction accuracy needs to be improved further, but it still could be considered as an acceptable model if we consider the nonlinearity and uncertainty of apartment heating energy usage in a district heating system.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.2
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• pp.191-196
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• 2005

Although the technology for emotion recognition is important one which was demanded in various fields, it still remains as the unsolved problems. Especially, there is growing demand for emotion recognition technology based on racial image. The facial image based emotion recognition system is complex system comprised of various technologies. Therefore, various techniques such that facial image analysis, feature vector extraction, pattern recognition technique, and etc, are needed in order to develop this system. In this paper, we propose new emotion recognition system based un previously studied facial image analysis technique. The proposed system recognizes the emotion by using the fuzzy classifier. The facial image database is built up and the performance of the proposed system is verified by using built database.

• Journal of the Korean Society of Radiology
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• v.4 no.3
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• pp.31-37
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• 2010

In this paper, a photovoltaic system is designed with PWM(Pulse Width Modulation) voltage source inverter. Proposed synchronous signal and control signal was processed by 56F8323 microprocessor for stable modulation. The PWM voltage source inverter using inverter consists of complex type of electric power converter to compensate for the defect, that is solar cell cannot be developed continuously by connecting with the source of electric power for ordinary use. It can cause the effect of saving electric power, from 10 to 20[%]. The PWM voltage source inverter operates in situation that its output voltage is in same phase with the utility voltage. In addition, I connected extra power to the system through operation the system voltage and inverter power in a synchronized way by extracting the system voltage so that the phase of the system and PWM voltage inverter can be synchronized. In the system of this research showed good results after being controlled in order to provide stable power to the load and the system through maintaining and low output power of harmonics.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.3 s.96
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• pp.251-258
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• 2005

The free vibration of a spinning flexible disk-spindle system supported by hydro dynamic bearings (HDB) in an HDD is analyzed by FEM. The spinning flexible disk is described using Kirchhoff plate theory and von Karman non-linear strain, and its rigid body motion is also considered. It is discretized by annular sector element. The rotating spindle which includes the clamp, hub, permanent magnet and yoke, is modeled by Timoshenko beam including the gyroscopic effect. The flexible supporting structure with a complex shape which includes stator core, housing, base plate, sleeve and thrust pad is modeled by using a 4-node tetrahedron element with rotational degrees of freedom to satisfy the geometric compatibility. The dynamic coefficients of HDB are calculated from the HDB analysis program, which solves the perturbed Reynolds equation using FEM. Introducing the virtual nodes and the rigid link constraints defined in the center of HDB, beam elements of the shaft are connected to the solid elements of the sleeve and thrust pad through the spring and damper element. The global matrix equation obtained by assembling the finite element equations of each substructure is transformed to the state-space matrix-vector equation, and the associated eigen value problem is solved by using the restarted Arnoldi iteration method. The validity of this research is verified by comparing the numerical results of the natural frequencies with the experimental ones. Also the effect of supporting structures to the natural modes of the total HDD system is rigorously analyzed.

• Computers and Concrete
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• v.19 no.3
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• pp.233-241
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• 2017

During the last two decades, CFRP have been extensively used for repair and rehabilitation of existing structures as well as in new construction applications. For rehabilitation purposes CFRP are currently used to increase the load and the energy absorption capacities and also the shear strength of concrete columns. Thus, the effect of CFRP confinement on the strength and deformation capacity of concrete columns has been extensively studied. However, the majority of such studies consider empirical relationships based on correlation analysis due to the fact that until today there is no general law describing such a hugely complex phenomenon. Moreover, these studies have been focused on the performance of circular cross section columns and the data available for square or rectangular cross sections are still scarce. Therefore, the existing relationships may not be sufficiently accurate to provide satisfactory results. That is why intelligent models with the ability to learn from examples can and must be tested, trying to evaluate their accuracy for composite compressive strength prediction. In this study the forecasting of wrapped CFRP confined concrete strength was carried out using different Data Mining techniques to predict CFRP confined concrete compressive strength taking into account the specimens' cross section: circular or rectangular. Based on the results obtained, CFRP confined concrete compressive strength can be accurately predicted for circular cross sections using SVM with five and six input parameters without spending too much time. The results for rectangular sections were not as good as those obtained for circular sections. It seems that the prediction can only be obtained with reasonable accuracy for certain values of the lateral confinement coefficient due to less efficiency of lateral confinement for rectangular cross sections.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.7
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• pp.78-86
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• 2010

This paper discusses the programming model of SODA-II that is a baseband processor for software defined radio (SDR) systems. Signal processing On-Demand Architecture Ⅱ (SODA-II) is an on-chip multiprocessor architecture consisting of four processor cores and each core has both an wide SIMD datapath and a scalar datapath. This architecture is appropriate for baseband processing that is a mixture of vector computations and scalar computations. The programming model of the SODA-II is based on C library routines. Because the library routines hide the details of complex SIMD datapath control procedures, end users can easily program the SODA-II without deep understanding on its architecture. In this paper, we discuss the details of library routines and how these routines are exploited in the implementation of baseband signal processing algorithms. As application examples, we show the implementation result of W-CDMA multipath searcher and OFDM demodulator on the SODA-II.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.2
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• pp.127-131
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• 2003

Protein disulfide isomerase (PDI) found in the endoplasmic reticulum (ER) catalyzes disulfide bond exchange and assists in protein folding of newly synthesized proteins. PDI also functions as a molecular chaperone and has been found to be associated with proteins in the ER. In addition, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins. A cDNA that encodes protein disulfide isomerase was previously isolated from Bombyx mori (bPDI), in which open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and an ER retention signal of the KDEL motif at its C-terminal, and we report its functional characterization here. This putative bPDI cDNA is expressed in insect Sf9 cells as a recombinant proteins using baculovirus expression vector system. The bPDI recombinant proteins are successfully recognized by antirat PDI antibody, and shown to be biologically active in vitro by mediating the oxidative refolding of reduced and scrambled RNase. This suggests that bPDI may play an important role in protein folding mechanism of insects.