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Identification and Cloning of a Fraction 1 Protein of Yersinia pestis that Produces Protective Immune Responses  

Kim Jong-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Cho Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Jang Hyun-Chul (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Lee Hee-Cheul (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Kim Young-Il (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Kang Yeon-Ho (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Lee Bok-Kwon (Division of Enteric Bacterial Infections, Center for Infectious Diesease, National Institute of Health)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.8, 2006 , pp. 1180-1184 More about this Journal
Abstract
The capsule that surrounds Yersinia pestis cells is composed of a protein-polysacchride complex; the purified protein component is fraction I (F1) antigen. We report the cloning of the cafl gene and its expression in Escherichia coli using the vector pETl02/D-TOPO and the F1-specific monoclonal antibody. The recombinant F1 (rF1) antigen had a molecular size of 17.5 kDa, which was identical to that of the F1 antigen produced by Y. pestis. Recombinant F1 protein was found to react to polyclonal antiserum to Y. pestis Fl. Recombinant F1 was purified by ProBond purification system and induced a protective immune response in BALB/c mice challenged with up to 10$^5$ virulent Y. pestis. Purified rF1 protein was used in an ELISA to evaluate the ability of a method to detect antibodies to Y. pestis in animal sera. These results strongly indicated that the rF1 protein is a suitable species-specific immunodiagnostic antigen and vaccine candidate.
Keywords
Y. pestis; fraction I antigen;
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1 Anderson, G. W., P. L. Worsham, C. R. Bolt, G. P. Andrews, S. L. Welkos, A. M. Friedlander, and J. P. Burans. 1997. Protection of mice from fatal bubonic and pneumonic plague by passive immunization with monoclonal antibodies against the F1 protein of Yersinia pestis. Am. J. Trop. Med. Hyg. 56: 471-473   DOI
2 Baker, E. E., H. Sommer, L. E. Foster, E. Meyer, and K. F. Meyer. 1952. Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis.J. Immunol. 68: 131-145
3 Bennett, L. G and T. G. Tornabene. 1974. Characterization of the antigenic subunits of the envelope protein of Yersinia pestis. J. Bacteriol. 117: 48-55
4 Meyer, K. F. 1970. Effectiveness of live or killed plague vaccines in man. Bull. World Health Organ. 42: 653-666
5 Sambrook, J. and D. W. Russel (eds.). 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
6 Welkos, S. L., K. M. Davis, L. M. Pitt, P. L. Worsham, and A. M. Friedlander. 1995. Studies on the contribution of the F1-capsule-associated plasmid pFra to the virulence of Yersin ia pestis. Contrib. Microbiol. Immunol. 13: 299-305
7 Xu, W., L. Beati, and D. Raoult. 1997. Characterization of and application of monoclonal antibodies against Rickettsia africae, a newly recognized species of spotted fever group rickettsia. J. Clin. Microbiol. 35: 64-70
8 Kim, K.-Y., B.-S. Koo, D. Jo, and S.-I. Kim. 2004. Cloning, expression, and purification of exoinulinase from Bacillus sp. snu-7. J. Microbial. Biotechnol. 14: 344-349
9 Butler, T. 1984. Plague, pp. 834-838. In K. S Warren and A. A. Mahmoud (eds.), Tropical and Geographical Medicine. McGraw-Hill, New York, N.Y
10 Kim, J.-H., S.-K. Choi, W.-J. Lim, and H.-I. Chang. 2004. Protective effect of astaxanthin produced by Xanthophyllomyces dendrorhous mutant on indomethacininduced gastric mucosal injury in rats. J. Microbiol. Biotechnol. 14: 996-1003   과학기술학회마을
11 Cowling, P. and P. Moss. 1994. Infectivity of pneumonic plague. Br. Med. J. 309: 1369
12 Reed, L. J. and H. A. Muench. 1938. A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27: 493-497
13 Galyov, E. E., O. Y. Smirnov, A. V. Karlishev, K. I. Volkovoy, A. I. Denesyuk, I. V. Nazimov, K. S. Rubstov, V. M. Abramov, S. M. Dalvadyanz, and V. P. Zav'yalov. 1990. Nucleotide sequence of Yersinia pestis gene encoding FI antigen and the primary structure of the protein. FEBS Lett. 277: 230-232   DOI   ScienceOn
14 Ljungstrom, I., G. Huldt, and A. Voller. 1974. The effect of Eperythrozoon cocco ides infection on the number of plaque forming cells in mice. Acta Pathol. Microbial. Scand. Sect. B Microbiol. Immunol. 82: 911-913
15 Willians, J. E., L. Arntzen, G. L. Tyndal, and M. Isaacson. 1986. Application of enzyme immunoassays for the confirmation of clinically suspect plague in Namibia. Bull. World Health Organ. 64: 745-752
16 Devdariani, Z. L., M. S. Verenkov, V. A. Feodorova, N. S. Solodovnicov, and L. G. Belov. 1993. Identification of Yersinia pestis with varied plasmid composition using monoclonal and polyclonal fluorescent immunoglobulins. FEMS. Immunol. Med. Microbiol. 6: 31-35   DOI
17 Anderson, G. W., D. G. Heath, C. R. Bolt, S. L. Welkos, and A. M. Friedlander. 1998. Short and long-term efficacy of single-dose subunit vaccines against Yersinia pestis in mice. Am. J. Trop. Med. Hyg. 58: 793-799   DOI
18 Shepherd, A. J., D. E. Hummitzsch, P. A. Leman, R. Swanepoel, and L. A. Searle. 1986. Comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents. J. Clin. Microbiol. 24: 1075-8
19 Rodrigues, C. G., C. M. Carneiro, C. T. Barbosa, and R. A. Nogueira. 1992. Antigen F1 from Yersinia pestis forms aqueous channels in lipid bilayer membranes. Braz. J. Med. Biol. Res. 25: 75-79
20 Davis, K. M., D. L. Fritz, L. M. Pitt, S. L. Welkos, P. L. Worsham, and A. M. Friedlander. 1996. Pathology of experimental pneumonic plague produced by fraction 1-positive and fraction 1-negative Yersinia pestis in African green monkeys. Arch. Pathol. Lab. Med. 120: 156-163
21 Williamson, E. D., S. M. Eley, A. J. Stagg, M. Green, P. Russell, and R. W. Titball, 1997. A sub-unit vaccine elicits IgG in serum, spleen cell cultures and bronchial washings and protects immunized animals against pneumonic plague. Vaccine 15: 1079-1084   DOI   ScienceOn