• Molecules and Cells
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• v.43 no.6
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• pp.501-508
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• 2020

In eukaryotes, membraneous cellular compartmentation essentially requires vesicle trafficking for communications among distinct organelles. A donor organelle-generated vesicle releases its cargo into a target compartment by fusing two distinct vesicle and target membranes. Vesicle fusion, the final step of vesicle trafficking, is driven intrinsically by complex formation of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). Although SNAREs are well-conserved across eukaryotes, genomic studies revealed that plants have dramatically increased the number of SNARE genes than other eukaryotes. This increase is attributed to the sessile nature of plants, likely for more sensitive and harmonized responses to environmental stresses. In this review, we therefore try to summarize and discuss the current understanding of plant SNAREs function in responses to biotic and abiotic stresses.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.11
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• pp.129-139
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• 2000

The problem of eigenvalue and eigenvector is obtained from a V-notched crack in pseudo-isotropic dissimilar materials by the traction free boundary and the perfect bonded interface conditions. The complex stress function is assumed as the two-term William's type. The eigenvalue is solved by a commercial numerical program, MATHEMATICA to discuss stress singularities for V-notched cracks in pseudo-isotropic dissimilar materials. The RWCIM(Reciprocal Work Contour Integral Method) is applied to the determination to eigenvector coefficients associated with eigenvalues. The RWCIM algorithm is also coded by the MATHEMATICA.

• BMB Reports
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• v.42 no.11
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• pp.719-724
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• 2009

Recent studies have revealed that endoplasmic reticulum (ER) disturbance is involved in the pathophysiology of neurodegenerative disorders, contributing to the activation of the ER stress-mediated apoptotic pathway. Therefore, we investigated here the molecular mechanisms underlying the ER-mitochondria axis, focusing on calcium as a potential mediator of cell death signals. Using NT2 cells treated with brefeldin A or tunicamycin, we observed that ER stress induces changes in the mitochondrial function, impairing mitochondrial membrane potential and distressing mitochondrial respiratory chain complex Moreover, stress stimuli at ER level evoked calcium fluxes between ER and mitochondria. Under these conditions, ER stress activated the unfolded protein response by an overexpression of GRP78, and also caspase-4 and-2, both involved upstream of caspase-9. Our findings show that ER and mitochondria interconnection plays a prominent role in the induction of neuronal cell death under particular stress circumstances.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.1
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• pp.15-22
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• 2007

A hybrid experimental-numerical method is presented for determining the stresses around a circular hole in a finite-width, tensile loaded plate. Measured fringe orders along straight lines provided the input information on the external boundary of the hybrid element. In order to see the effects of varying stress field, different numbers of terms in a power-series representation of the complex type conformal mapping stress function were tested. For qualitative comparison, actual isochromatic fringes were compared with reconstructed theoretical fringes using stress-optic law. For quantitative comparison, relative errors and standard deviations with respective to relative errors were analyzed for all measured points by changing the number of terms of stress function. The hybrid results are highly comparable with those predicted by FEA. The results show that this approach is effective and promising because isochromatic data along the straight lines in photoelasticity can be conveniently measured by use of phase shifting photoelasticity.

• Clinical and Experimental Pediatrics
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• v.57 no.10
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• pp.425-433
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• 2014

Adrenal and thyroid hormones are essential for the regulation of intrauterine homeostasis, and for the timely differentiation and maturation of fetal organs. These hormones play complex roles during fetal life, and are believed to underlie the cellular communication that coordinates maternal-fetal interactions. They serve to modulate the functional adaptation for extrauterine life during the perinatal period. The pathophysiology of systemic vasopressor-resistant hypotension is associated with low levels of circulating cortisol, a result of immaturity of hypothalamic-pituitary-adrenal axis in preterm infants under stress. Over the past few decades, studies in preterm infants have shown abnormal clinical findings that suggest adrenal or thyroid dysfunction, yet the criteria used to diagnose adrenal insufficiency in preterm infants continue to be arbitrary. In addition, although hypothyroidism is frequently observed in extremely low gestational age infants, the benefits of thyroid hormone replacement therapy remain controversial. Screening methods for congenital hypothyroidism or congenital adrenal hyperplasia in the preterm neonate are inconclusive. Thus, further understanding of fetal and perinatal adrenal and thyroid function will provide an insight into the management of adrenal and thyroid function in the preterm infant.

• Transactions of the Korean Society of Mechanical Engineers
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• v.15 no.5
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• pp.1530-1537
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• 1991

본 연구에서는 반무한 직선 계면균열의 상하면에 임의로 분포하는 어떠한 하 중에 대해서도 그 해석이 가능한 그린함수(Green's function)를 구하고자 한다. 이 를 위하여 반무한 직선 계면균열상의 임의의 한 점에 평면 집중하중이 작용하는 문제 와 비평면 집중전단하중이 작용하는 문제를 각각 택하였고, 이때 계면균열의 선단은 열려있다고 가정하였다. 이 문제를 풀므로써 균열선단부근의 응력성분을 결정하고 이로부터 그린함수의 의미를 지니는 응력강도계수에 대한 폐형해를 얻었다.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.11
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• pp.1187-1194
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• 2009

During the last decade, possibility of flaw occurrences has been rapidly increased world-widely as the increase of operating times of petro-chemical facilities. For instance, from a recent in-service inspection, three different sized surface cracks were detected in welding parts of a spherical oxygen holder in Korea. While API579 code provides corresponding engineering assessment procedures to determine crack driving forces, in the present work, numerical analyses are carried out for the cracked oxygen holder to investigate effects of complex geometry, analysis model and residual stress. With regard to the detailed finite element analysis, stress intensity factors are determined from both the full three-dimensional model and equivalent plate model. Also, as an alternative, stress intensity factors are calculated for equivalent plate model by employing the noted influence stress function technique. Finally, parametric structural integrity evaluation of the cracked oxygen holder is conducted in use of failure assessment diagram method, J/T method and DPFAD method. Effects of the geometry and so forth are examined and key findings from the simulations are fully discussed, which enables to determine practical safety margins of spherical components containing a defect.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.2
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• pp.239-246
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• 2003

This paper proposes engineering estimation equations of elastic-plastic J and COD fur axial through-wall cracked pipes under internal pressure. Based on detailed 3-D FE results based on deformation plasticity, the plastic influence functions for fully plastic J and COD solutions are tabulated as a function of the mean radius-to-thickness ratio, the normalized crack length. and the strain hardening. Based on these results, the GE/EPRI-type J and COD estimation equations are proposed and validated against the 3-D FE results based on deformation plasticity. For more general application to general stress-strain laws or to complex loading, the developed GE/EPRI-type solutions are re-formulated based on the reference stress concept. Such a reformulation provides simpler equations for J and COD, which are then further extended to combined internal pressure and bending. The proposed reference stress based J and COD estimation equations are compared with elastic-plastic 3-D FE results using actual stress-strain data for Type 316 stainless steels. The FE results for both internal pressure cases and combined internal pressure and bending cases compare very well with the proposed J and COD estimations.

• Journal of Dental Rehabilitation and Applied Science
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• v.23 no.4
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• pp.373-384
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• 2007

The purpose of this study was to analyse the stress distribution on the craniofacial suture and cranium after application of RME. Twelve years and six months old boy and twenty years old adult male were chosen for taking computed-tomography for FEM. From DICOM visual information, it was processed by 3-dimensional image construction program Mimics 10.01. Hounsfield unit(HU) which shows gray scale of CT image is picked for revealing mechanical properties of each model. The models have been accomplished with various range of physical properties. After applying 5.0 mm expansion, the maxillary complex model was obeserved for analyzing displacement and stress distribution of the model. The amount of transverse expansion of child and adult maxilla is different according to its location. It appears that it decreases gradually with the distance from separation site. In child, maximum compressive stress located broad area in zygomatic buttress department and the ends of frontal process of maxilla, pterygoid plate, and bones surrounding orbit. However, in adult maximum compressive stress was located smaller area and the stres was higher than child.

• Applied Microscopy
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• v.38 no.3
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• pp.213-219
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• 2008

The plasticity of nervous system is generated not only due to changes in neurons but also due to changes in neuroglial cells. Astrocyte is important for maintaining the normal brain function and controlling the neuronal functions. The amygdala receives an array of important sensory information of danger signals. This information is further transduced and integrated to produce the highly adaptive emotion, fear. In this study, morphometric changes in the cell bodies of astrocytes in the amygdala, induced by prenatal stress and restraint stress were examined. For this purpose. rats were classified into 4 groups; control group (CON), only restraint-stressed (starting on P90 for 3 days) group (CONR), prenatally-stressed group (PNS), and prenatally and restraint (on P90 for 3 days) stressed group (PNSR). Astrocytes were verified with anti-GFAP immunohistochemistry, counter stained with methylene blue/azure II and were examined using the Neurolucida. Results showed that astrocytes in the amygdala of PNS rats had significantly larger cell bodies than did CON rats and this was enhanced further by restraint stress. Thus this data showed that hypertrophy of the astrocytic cell bodies of amygdala complex is induced by prenatal and restraint stress.