• Fisheries and Aquatic Sciences
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• 제27권3호
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• pp.159-170
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• 2024

Research to obtain molecular markers related to the major histocompatibility complex (MHC) gene in both strains of gourami is essential to increase the success of the selection program of disease resistance traits. Using a completely randomized design (CRD), the challenge test consists of four treatments and seven replications. The treatment was Jambi gourami injected with PBS (KJ), Kalimantan gourami injected with PBS (KK), Jambi strain injected with Aeromonas hydrophila (GJ), and Kalimantan strain injected with A. hydrophila (GK). The GJ population was more resistant to A. hydrophila than the GK population. The MHC II gene was detected in both test strains (GJ and GK), both resistant and susceptible fish. However, there were differences in the results of amplifying the MHC II gene in susceptible and resistant fish. Two DNA fragments approximately 400 and 585 bp were detected in the genome of susceptible fish, while in the genome of susceptible fish, only one DNA fragment was detected (400 bp). Therefore, the MHC II gene fragment with a size of about 585 bp can be used as a potential candidate for specific molecular markers to obtain resistance to A. hydrophila bacteria in the giant gourami.

• Journal of Preventive Medicine and Public Health
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• 제32권4호
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• pp.473-481
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• 1999

Objectives: In recent days, the problem of odor pollution in community near Sihwa Industrial complex, Kynuggi Province is becoming of significant public concern. We have investigated the health effects of the Sihwa residents from odor pollution comparing with other less polluted areas. Methods: The Ansan and Kuri Cities were selected as control areas. The parents of the elementary and middle school students in these three areas were surveyed with structured questionnaire twice, Nov 1997 and Jure 1998 each. As a exposure index, the ambient air concentrations of five major air pollutants(particulates, $O_3,\;SO_2,\;NO_2$, CO) and subjective odor perception were used. We have focused health outcomes such as the prevalence of nonspecific irritant symptoms, respiratory disease among family members and the score of qualify of life(QOL). Results: Although the mean concentrations of major air pollutants except particulates were similar or lower in Sihwa than other areas, the odor perception rate and the monthly odor perception days were significantly higher. It suggested that odor producing chemical compounds are the major source of environmental pollution problem. There were higher prevalence rates of nonspecific irritant symptoms and respiratory disease among family members in Sihwa than other control areas. The QOL score was also lower in Sihwa. The odor perception proved to be a most important factor in reporting adverse health effects and lowering the QOL score. Conclusion: The residents living near Sihwa industrial complex were suffering from more adverse health symptoms and poorer QOL status than control areas. And it may be due to environmental odor pollution from industrial complex. Therefore, further research will be needed for monitoring of the responsible chemicals emitted from industries.

• BMB Reports
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• 제45권6호
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• pp.331-336
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• 2012

The retinoid-related orphan nuclear receptor gamma ($ROR{\gamma}$) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the $ROR{\gamma}$ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro $ROR{\gamma}$-CTAP(SG), the nuclear localization of $ROR{\gamma}$-CTAP(SG) fusion protein was verified. Following isolation of $ROR{\gamma}$ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with $ROR{\gamma}$ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the $ROR{\gamma}$ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with $ROR{\gamma}$ protein in a complex format and function as co-activators in the $ROR{\gamma}$-mediated regulatory processes of HepG2 cells.

• 대한의생명과학회지
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• 제24권4호
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• pp.372-379
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• 2018

Korean society is experiencing rapid growth due to industrialization and urbanization, and the aging of population is emerging. Degradation of the masticatory muscle due to aging and reduction of the number of mastications causes dry mouth syndrome with decreased saliva in the elderly. Changes in the amount and composition of saliva cause various oral diseases, especially, periodontal disease is closely related to the microorganisms that form the plaque. In the elderly education, stimulation saliva was collected at the first visit and the last visit to observe changes in microorganisms in the oral cavity. For the collection of the probes, the paraffin wax was chewed for 5 minutes. The samples were immediately refrigerated in a 50 cc plastic tube and then stored at $-20^{\circ}C$ until the next use. The subjects of this study were the elderly people aged 65 years or older, and the study was conducted on 16 persons, 3 males and 13 females. The distribution of saliva microorganisms according to rolling brushing method showed significant difference in all three groups (Aa & Red complex, Orange complex and Green complex) before and after the education. In the group who answered that they had dentures, it was confirmed that the amount of microorganisms before and after education was significantly reduced in all three groups. There was a significant decrease in the amount of microorganisms in the Aa & Red complex and Orange complex group in the case of hypertension related to systemic disease. In conclusion, the higher the interest of oral health and general health of the subjects, the more effective the education.

• 대한간호학회지
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• 제50권5호
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• pp.699-713
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• 2020

Purpose: The purpose of this study was to understand and describe the experiences of patients with Meniere disease. Methods: Data were collected from February 19, 2019, to February 5, 2020, through individual in-depth interviews with 13 Meniere patients. Verbatim transcripts were analyzed using Colaizzi's phenomenological analysis. Results: The experiences of patients with Meniere disease were clustered into the following four themes from 22 meaning units: 1) Complex unfamiliar symptoms that shatter both balance of the body and peace of the heart; 2) A disease that medical treatment and health professionals cannot cure; 3) Suffering hardships that cannot be understood by non-Meniere sufferers; and 4) Making daily efforts to become healthier. Symptoms of Meniere disease are life-shattering and depressing because they are neither visible nor easily curable. Over time, as they accepted the reality of living with the disease, the participants would shift their focus from complete symptomatic cure to leading a healthy and more balanced life. Conclusion: This study shows that Meniere disease has a pervasive impact on all aspects of the patients' lives. Patients are prone to experiencing restrictions in their social functioning and activities. They also experience psychosocial problems due to the unseen nature of their symptoms. This study elucidates the experiences of Meniere patients and the need for nursing intervention to help improve their quality of life and ability to self-manage. Lastly, this study shows the need for a coordinated interdisciplinary approach to raising public awareness of the disease.

• 생명과학회지
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• 제25권8호
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• pp.932-935
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• 2015

본 연구는 30대 비만 남성을 대상으로 복합트레이닝 처치가 염증지표와 심혈관질환의 지표물질인 homocysteine에 미치는 영향을 규명하는데 목적이 있다. 본 연구의 대상자는 체지방률이 25% 이상인 30대 중반의 비만 남성(n=12)으로 의학적인 질환이 없고, 규칙적인 운동경험이 없는 자를 선정하였다. 본 연구의 목적을 달성하기 위하여 기본검사로 최대산소섭취량(VO_2max)과 5가지 기구의 1RM을 측정하였고, 이 결과를 토대로 유산소운동과 중량운동으로 구성된 복합트레이닝을 8주간 실시하여 트레이닝 전과 후에 체구성(체중, 체지방, BMI)과 혈액 내 염증지표(IL-6, TNF-α, CRP) 및 homocysteine의 변화를 분석하였다. 그 결과, 8주간의 복합트레이닝은 체중, 체지방, BMI를 유의하게 감소시켰으며(p<0.01), 염증지표 CRP와 심혈관질환지표 homocysteine을 유의하게 감소시켰다(p<0.05). 따라서 8주간의 복합트레이닝 처치를 통해 체구성의 변화를 확인할 수 있었고, 이러한 변화가 염증지표와 심혈관질환지표에 긍정적인 영향을 미친 것으로 사료된다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2004년도 International Meeting of the Microbiological Society of Korea
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• pp.45-46
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• 2004

• 대한한의학방제학회지
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• 제21권2호
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• pp.72-80
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• 2013

Objectives : Ontology is a good tool to represent the knowledge and has developed for Traditional Korean Medicine(TKM) in Korea Institute of Oriental Medicine. There are a lot of TKM terms, which have a complex meaning, especially major indication terms of medicinal treatment and terms of symptom and disease. These complex meaning terms result in the low linkage between major indication terms of medicinal treatment and terms of symptom and disease in TKM ontology. We studied to enhance the percentage of the linkage among those data in TKM ontology. Methods : We disassembled major indication terms of medicinal treatment into minimum meaning units and then linked them to enhance the percentage of the linkage among medicinal material, formula and disease ontology based on Traditional Korean Medicine. To retain objectivity, several experts of Korean Medicine used a web-based tool that supports users in refining terms and disassembling them into the minimum meaning efficiently. Results : The outcome shows that the percentage of the linkage among medicinal material, formula and disease ontology increased. By linking disassembled major indication terms to symptoms and diseases, the amount of information for medicinal materials and formulas also increased quantitatively in comparison with given formula for diseases in disease ontology. Conclusions : By this study, disassembled data increases the percentage of linkage between diseases and medicinal treatments. And the applicability of TKM ontology is also increased.

• 과학기술학연구
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• 제8권2호
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• pp.27-56
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• 2008

미국 쇠고기 수입 재개 결정으로 발생한 광우병 논란은 기술위험 관리의 차원에서 볼 때 중요한 사례라고 할 수 있다. 광우병은 새로운 수입 조건하에서 충분히 통제될 수 있으며 감내할 만한 수준이라는 정부의 거듭된 주장에도 불구하고 심각한 위험으로 인식하는 국민들은 수입조건의 재협상을 요구하면서 격렬한 대립이 초래되었다. 예방이나 치료법이 없으며 치사율도 매우 높기 때문에 심각한 위험으로 인식될 가능성이 높은 상황에서 내려진 비일관적 결정이 문제 발생의 원인이었다. 또 정부와 관련 제도에 대한 신뢰의 부족도 정부의 결정을 수용할 수 없는 중요한 조건이었다. 그 결과 국민적 저항이 확산되고 이명박 정부의 위기로까지 이어졌다. 이명박 정부의 주장처럼 위험문제의 정치화는 회피되어야할 것이 아니라 오히려 공개적인 토론과 타협을 요구한다. 새로운 유형의 기술위험이 지속적으로 등장하고 있으며 이러한 논란이 되풀이 될 가능성도 있다는 점에서 기술위험 관리에 대한 새로운 접근과 대응이 필요하다. 광우병 논란은 기술위험 관리의 실패라는 점에서 위험관리에 많은 함의를 제공해 주고 있다.

• Clinical and Experimental Reproductive Medicine
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• 제38권1호
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• pp.42-46
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• 2011

Objective: This study was aimed to investigate endometrial histology and to find predictable clinical factors for endometrial disease (hyperplasia or cancer) in women with polycystic ovary syndrome (PCOS). Methods: We investigated the endometrial histology and analyzed the relationship between endometrial histology and clinical parameters, such as LH, FSH, estradiol, testosterone, fasting and 2 hours postprandial glucose and insulin, insulin resistance, body mass index, endometrial thickness, menstrual status from 117 women with PCOS. Statistical analysis was performed with chi square and t-test, p-value<0.05 was considered as statistically significant. And receiver operating characteristic curve was used to find predictable clinical factors for endometrial disease and to decide the cuff off values. Results: In 117 women with PCOS, endometrial histologic profiles are as follows: proliferative phase in 90 women (76.9%), endometrial hyperplasia in 25 women (21.4%), and endometrial cancer in 2 women (1.7%). Of 25 women with endometrial hyperplasia, simple hyperplasia without atypia, complex hyperplasia without atypia and complex hyperplasia with atypia were diagnosed in 15 (12.8%), 6 (5.1%), 4 (3.4%) women, respectively. Age and endometrial thickness were significantly related with endometrial disease, p=0.013 and p=0.001, respectively. At the cut off level of 25.5 years in age, sensitivity and specificity predicting for endometrial disease were 70.4% and 55.6%, respectively (p=0.023). At the cut off level of 8.5 mm in endometrial thickness, sensitivity and specificity were 77.8% and 56.7%, respectively (p=0.000). Conclusion: In women with PCOS, the incidence of endometrial hyperplasia and cancer were 21.4% and 1.7%. The age and endometrial thickness may be used as clinical determining factors for endometrial biopsy.