• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.67-75
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• 2002

The human complement receptor type 1 (CR 1, C3 b/C4b receptor) is a polymorphic membrane glycoprotein expressed on human erythrocytes, peripheral leukocytes, plasma and renal glomerular podocytes, which consists of transmembrane and cytoplasmic domains with 30 repeating homologous protein domains known as short consensus repeats (SCR). CR1 has been used as an inhibitor for inflammatory and immune system for the past several years. Recently; it is reported that CRl was found to suppress the hyper-acute rejection in xeno-transplantation and can be used to cure autoimmune diseases. A soluble form of CRl, called sCRl, is a recombinant CRl by cleaving the transmembrane domain at C-terminus and has been expressed in Chinese Hamster Ovary (CHO) cells. Several purification methods for sCR1 from CHO cells have been reported, but most of them require complicated steps at high cost. Moreover, such methods are mostly performed under the pH condition apt to denaturing sCR1 and causes sCRl losing its activity. We here report a rapid and efficient method to purify sCR1 from CHO cell. The new method consists of a two-stage of cell culture by cultivating cells in serum medium followed by serum-free medium, and a two-stage of column purification by means of heparin and gel filtration column chromatography. By using this novel method, sCR1 can be purified in a simple and effective way with high yield and purity, furthermore, the purified sCR1 was confirmed to retain its activity to suppress the complement activation in vivo and ex vivo.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(3)
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• pp.157-160
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• 2000

Nowadays, internet or web is popularized in society. So, intranet or inside computation environment of business has been changed a web based open system. Client and Server system that has been used core technique for enterprise information system several years is developing e-business system that perform a business in web based. Because, internet is extension of a whole field like business or information technology Therefore we understand a meaning of e-business and ERP system that a form of e-business system. Then we realize problem of existing system and study a technology for complement of system. Finally, we propose a proper model for e-business system and develop an example.

• 한국시스템다이내믹스연구
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• 제14권1호
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• pp.89-104
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• 2013

This study aims to explore the potentials of System Dynamics as a tool to understand the community welfare practice by paying attention to the strengths of System Dynamics to complement the weaknesses of the theories and studies of the community welfare practice. The contents of this study are as follows. First, it draws the limitations of its theories and studies by reviewing the literatures related. Second, it outlines the characteristics of the community welfare practice process as well as the strengths of System Dynamics as a tool to understand the community welfare practice. Third, it presents a causal loop model of System Dynamics applied to the community welfare practice process. Lastly, it concludes with the suggestions and implications of the key research findings. This study is expected to be a basic study to highlight the potentials of System Dynamics as a tool to understand the community welfare practice.

• Journal of Nutrition and Health
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• 제16권3호
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• pp.193-199
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• 1983

Clinical and epidemiologic data point to a causal interrelationship between nutritional deficiency and infectious illness. Both are major contributors to childhood morbidity and mortality, particulary in underprivileged population groups. Protein-calorie malnutrition depress a variety of immune funtions. Delayed hypersensitivity and number of T-lymphocytes are consistently reduced. The interrelationship between nutritional status and immune response was studied in 80 children aged under 6 years. According to the anthropometric assessment based on weight for height for age, 36 children were classified as normal, 22 as morderate malnutrition, and 22 as severe malnutrition. The following determinations were made : hemeglobin, hematocrit, serum albumin, immunoglobulin G, complement 3, and WBC levels. Results indicate that levels of Hb, Hct, serum albumin, and C3 concentration were decreased in moderate and severe malnutrition children. However, levels of IgG and WBC were not affected by the nutritional status. It is suggested that nutritional status has more profound effects on complement system than humoral immunity.

• 한국미생물·생명공학회지
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• 제22권4호
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• pp.360-367
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• 1994

The effect of various nutrients for the production of the anti-complementary polysaccha- ride by Flammulina velutipes, was examined and the partial purification of the polysaccharide was carried out. The culture conditions and medium compositions considerably influenced the anti-complementary activity of the polysaccharides. When a culture was carried out at 23$\circ$C and pH 5.5 for 6 days in the synthetic medium supplemented with galactose and NaNO$_{3}$, the production of the anti-complementary polysaccharide was maximized. The crude polysaccharide, FV-1 was isolated from the culture broth and partially purified into two fractions, FV-1-II and FV-1-III by gel filtration using Sephadex G-100. FV-1-II was mainly consisted of xylose, mannose, galactose and glucose, and rhamnose, mannose and galactose, for FV-1-III. Also, the anti-complementary activity of FV-1-III was reduced partially in the absence of the Ca ion. When crossed immunoelect- rophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the FV-1-III in the Ca ion free condition, a cleavage of C3 precipitin line was observed. These results indicate that the mode of complement activation by polysaccharide from Flammulina velutipes is via not only the classical pathway but also the alternative pathway.

• 한국식품영양학회지
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• 제7권3호
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• pp.159-168
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• 1994

From the hot water extract of bracken(Pteridium aquilinum var. latiusculum), a Korean win edible plant, anti-complementary acidic polysaccharides were Isolated. Crude polysaccharide fraction(HPA-1) was obtain ed by methanol reflux, ethanol precipitation, dialysis, and lyophilization. HPA-1 contained 81.80% of total sugar, 30.40% of uronic acid, and 15.60cA of protein. HPA 1 was purified consecutively by cetavlon fractionation and chromatography including ion exchange nth DEAE-Sepharose CL 6B and gel permeation with Sephadex G-100 and Sepharose CL-6B. HPA-2- IVa and HPA-Va-2 were nearly homogeneous on HPLC and had 500,000 and 560,000 daltons of molecular weights, respectively. HPA-2-Wa consisted of fucose, galacturonic acid, and glucuronic acid at the molar ratio of 1.40 : 0.97 : 1.88. HPA-2-Va 2 was composed of rhamnose, galactose, and galacturonic acid at the molar ratio of 1.00 : 1.38 : 1.39. The polysaccharides were found to activate the C3 component both In the presence and In the absence of Ca2+ through the crossed-immunoelectrophoresis suggesting that those Involved in both classical and alternative complement pathway.

• Industrial Engineering and Management Systems
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• 제11권4호
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• pp.346-352
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• 2012

While systems engineering has been widely applied to complex system development, some evidences are reported about major budget and schedule overruns in systems engineering applied. On the other hand, many organizations have been deploying Design for Six Sigma (DFSS) to build Six Sigma momentums in the area of design and development for their products and processes. To explore the possibility of having a DFSS complement systems engineering process, this process reviews the systems engineering with their categories of effort and DFSS with its methodologies. A comparison of the systems engineering process and DFSS indicates that DFSS can be a complement to systems engineering for delivering higher quality products to customers faster at a lower cost. We suggest a simplified framework of systems engineering process, that is, PADOV which was derived from the generic systems engineering process which has been applied to the development of T-50 advanced supersonic trainer aircraft by Korea Aerospace Industries (KAI) with technical assistance of Lockheed Martin. We demonstrated that each phase of PADOV framework is comprehensively matched to the pertinent categories of systems engineering effort from various standards.

• 통상정보연구
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• 제6권3호
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• pp.137-157
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• 2004

It is very necessary to find how to develop international government procurement by analyzing functions and roles of information providing circumstance and existing total international government procurement information system. In addition, it would require to draw up a way of improving current international government procurement information system based on the problems of existing system. In this study, we analyze the current status of Korean international government procurement information system and give an idea of mutual cooperating with DGMARKET, a good example of international government procurement information system. As a practical method, cooperating with DGMARKET by building each countries' gateway will be very helpful in cost saving and providing information. It will also complement the limitation of Korean system's one way service. But supplying only international government procurement information is insufficient in realization of e-trade in international goverment procurement market. To settle these problems, treating international government procurement as a same field of trade and giving political support and incentive to company which hopes to participate international goverment procurement are absolutely needed.

• 한국기계가공학회지
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• 제10권6호
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• pp.39-45
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• 2011

Due to the introduction of the electronic control systems, agricultural machine is getting complicated with its special function. The complex electronics system increases difficulty of maintenances in the wiring harness system. The heavy wiring harness has some poor reliability and workability in the production line of manufacture also. So, in this study, some small local control modules are developed to complement the difficulties with the benefits of distributed control system. All of the local control modules are connected by using CAN communication. This system developed this study has the advantages of modification or alteration of wiring and local modules. All the sensors and actuators can be easily monitored and controlled by the main controller with the appropriate communication protocol made by in this study.

• 한국정보처리학회논문지
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• 제7권9호
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• pp.2876-2884
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• 2000

단문분할은 한 문장에 용언이 복수개 있을 때 용언을 중심으로 문장을 나누는 방법이다. 기존의 방법은 정형화된 문장의 경우 비교적 효율적인 결과를 얻을 수 있으나, 구문적으로 복잡한 문장인 경우는 한계를 보였다. 본 논문에서는 이러한 한계를 극복하기 위해서 구문 정보만이 아니라, 의미 정보를 활용하여 단문을 분할하는 방법을 제안한다. 정형화된 문장의 경우와 달리 일상적인 문장은 무장 구조의 모호성이나 조사의 생략 등이 빈번하므로 의미 수준에서의 단문분할이 필요하다. 의미 영역에서 단문분할을 하면 기존의 구문 의존적인 방법들에서 발생하는 모호성을 상당수 해소할 수 있게 된다. 논문에서는 먼저 하위범주와 사전과 시소러스의 의미 정보를 이용하여 용언과 보어성분 간의 의존구조를 우선적으로 파악하고, 구문적인 정보와 기타 문법적인 지식을 사용하여 기타 성분을 의존구조에 점진적으로 포함시켜가는 이단계 단문분할 알고리즘을 제안한다. 제안된 이단계 단문분할 방법의 유용성을 보이기 위해 ETRI-KONAN의 말뭉치 중 25,000문장을 수작업으로 술어와 보어성분 간의 의존구조를 태깅한 후 본 논문에서 제안한 방법과 비교하는 실험을 수행하였으며, 이때 단문분할의 결과는 91.8%의 정확성을 보였다.