• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.465-470
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• 2007

To evaluate the effect of companion plant (Phlox subulata and Glechoma hederacea var. longituba) on conservation of soil in slopped highland, coverage of crops and soil erosion were investigated with 3 different conditions. The coverage rate of Phlox subulata with Chinese cabbage cultivation was kept on approximately 100% from Chinese cabbage-transplanting to -harvest. The coverage rate of Glechoma hederacea var. longituba with Chinese cabbage cultivation was low at it's early stage, however, reached to approximately 100% at the time of Chinese cabbage harvest. In contrast, the coverage rate of Chinese cabbage cultivation without cover crop, and simultaneous transplanting with Chinese cabbage and cover crop were approximately 60%. Losses of soil and nitrate nitrogen were much lower in Phlox subulata with Chinese cabbage cultivation ($0.1{\sim}0.2ton\;ha^{-1}$, $0.2{\sim}0.4kg\;NO_3{^-}-N\;ha^{-1}$) than those in Chinese cabbage cultivation without cover crop ($20.8ton\;ha^{-1}$, $2.1kg\;NO_3{^-}-N\;ha^{-1}$), and simultaneous transplanting with Chinese cabbage and cover crop ($8.9{\sim}10.5ton\;ha^{-1}$, $1.5{\sim}2.2kg\;NO_3{^-}-N\;ha^{-1}$). Cover plants suppressed the weed occurrence up to about 70%. Live mulching with cover plants set a good effects on weed suppression and reduction of soil and nutrient loss. Therefore intercropping with Phlox subulata will make great contributions to soil conservation in Chinese cabbage cultivated highland.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1165-1176
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• 2019

Botulinum neurotoxins (BoNTs), produced by Clostridium botulinum, are the most toxic substances known. However, the number of currently approved medical countermeasures for these toxins is very limited. Therefore, studies on therapeutic antitoxins are essential to prepare for toxin-related emergencies. Currently, more than 10,000 Halla horses, a crossbreed between the native Jeju and Thoroughbred horses, are being raised in Jeju Island of Korea. They can be used for equine antitoxin experiments and production of hyperimmune serum against BoNT/A1. Instead of the inactivated BoNT/A1 toxoid, Halla horse was immunized with the receptor-binding domain present in the C-terminus of heavy chain of BoNT/A1 (BoNT/A1-HCR) expressed in Escherichia coli. The anti-BoNT/A1-HCR antibody titer increased rapidly by week 4, and this level was maintained for several weeks after boosting immunization. Notably, $20{\mu}l$ of the week-24 BoNT/A1-HCR(-immunized) equine serum showed an in vitro neutralizing activity of over 8 international units (IU) of a reference equine antitoxin. Furthermore, $20{\mu}l$ of equine serum and $100{\mu}g$ of purified equine $F(ab^{\prime})_2$ showed 100% neutralization of 10,000 $LD_{50}$ in vivo. The results of this study shall contribute towards optimizing antitoxin production for BoNT/A1, which is essential for emergency preparedness and response.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.255-264
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• 2018

This experiment was conducted to analyse the effects of flavone supplementation on the preimplantation development of in-vitro produced porcine embryos. During in-vitro development, immature oocytes and early embryos were exposed to different concentrations of flavone (0, $1{\mu}M$, $25{\mu}M$, $50{\mu}M$, and $100{\mu}M$ respectively). Results showed that $100{\mu}M$ of flavone significantly reduced the intracellular ROS levels of oocytes accompanied with a significant rise in GSH level. In parthenogenesis, no significant change was observed in the cleavage rates whether flavone was supplemented in IVM or IVC media. In IVM supplemented group, the blastocyst development rate was significantly enhanced by $1{\mu}M$ concentration than other groups (51.5% vs. 41.3%, 44.0%, 36.3%, 31.7%; P<0.05) respectively. However, in IVC group $1{\mu}M$ concentration significantly improved the blastocysts production than $50{\mu}M$ and control groups (50.0% vs. 40.5%, 38.0%; P<0.05) respectively. Following nuclear transfer, the cleavage rate of IVM group was significantly more in $1{\mu}M$ than $50{\mu}M$ and $100{\mu}M$ groups (92.9% vs. 89.7%, 87.8%; P<0.05), followed by similar pattern of cloned blastocysts production being significantly higher in $1{\mu}M$ group than $50{\mu}M$, $100{\mu}M$ and control groups (16.8% vs. 9.0%, 7.1%, 12.8%; P<0.05) respectively. In IVC group, $1{\mu}M$ concentration resulted in significantly higher cleavage rate than $25{\mu}M$ and $50{\mu}M$ groups (91.7% vs. 87.8%, 88.8%; P<0.05) respectively. However, the blastocysts production was significantly higher in $100{\mu}M$ group than others (26.2% vs. 13.6%, 14.0%, 18.2%; P<0.05) respectively. The optimal concentrations of flavone significantly enhanced the percentages of ICM:TE than control group (43.8% vs. 37.6%; P<0.05) accompanied with significantly higher expression levels of reprogramming related genes. In conclusion, the optimal concentrations of $1{\mu}M$ during IVM and $100{\mu}M$ during IVC can significantly improve the production of porcine in-vitro embryos.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.29 no.2
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• pp.28-38
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• 2011

Dwelling reflects the continuance and change that, in general, expresses the era's cultural and social values. The yard of Korean traditional dwelling, although it is an exterior space, it is a companion space that is engaged with the main house. It operates many housing functions that were not achieved within the interior space. After the time of enlightenment, shape, function and the meaning of Korean traditional house yard along with the change of values and major living style has been changing. Therefore, to understand how the Chosun Dynasty's house yard is being used in modern days, this study is based on the research of 47 sites and conducted a study regarding the acculturation and division the house yards. Typical elements in the target garden site that are expressed in a way differentiated from the original form of the Korean traditional house yard were the materials and methods of planting, the use of garden ornaments, paving materials, the use of water, and changes in kitchen gardens. As the role of house yards changes, the modern division of the style of gardens occurred; the areas in a garden have been divided and the form of a garden has been differentiated according to the limit and initiative. The pursuit of ornamentality and practicality led to the differentiation of gardens' functions, while seeking after convenience resulted in the division of gardens' materials. The use of gardens has also been differentiated by adding commerciality and a concept of open garden in both symbolic and daily manners.

• 한국노년학
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• v.30 no.1
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• pp.241-260
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• 2010

This study aims to fully understand the experience of elderly men living alone in a single room occupancy(Chokbang) by identifying the meaning and essence of their experiences. This research used purposive sampling. The data were collected for 7 months from september 2008 to march 2009. Eight elderly men participated in the interview. Mainly the semi-structured in-depth interview and focus group interview were used. The data analysis was based on Giorgi's 4 types of specific steps. As a result, 4 components and 16 subordinate components were drawn from the analysis. The components resulted from the analysis are: , , , . Based on these results, I discussed the attitudes of the elderly men living alone in Chokbang in meaningful and gender-sensitive ways. Moreover, I provided social welfare connotation and future research suggestions.

• The Journal of the Korea Contents Association
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• v.21 no.6
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• pp.248-260
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• 2021

This study assumes that the smooth school life of adolescents from multicultural families is recognized as an important part of multicultural support policies and practices as a basis for realizing an integrated society, and system elements that are meaningful for their flexibility to adapt to school (individuals, families, schools) By empirically analyzing the effect of, we tried to bring out highly effective implications for the composition of a multidimensional practice system. Therefore, this study is a causal model in which self-identity (individual factor) and parental parenting attitude (family factor), which are important variables for each system level, are set as independent variables, and school support (school factor) consisting of teacher support and companion support is set as control variables. After collecting data through a questionnaire survey by interviews with 162 middle and high school students in Gyeonggi and Gangwon regions, the effect was analyzed through multiple regression analysis. As a result of the analysis, it was confirmed that school support has a very important static moderating effect in the positive (+) effect of adolescents' self-identity and parenting attitude on school adaptability. It was confirmed that the modulating effect is relatively more important.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.495-501
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• 2021

This study examined the effects of pre-service teacher's cooperative organizational culture on the conflict management type. The subjects were 138 preschool teachers. The data were obtained by self-reporting and used to investigate the teacher's conflict management type and cooperative organizational culture. The collected data were analyzed using the Pearson correlation coefficient analysis and stepwise regression analysis. The major findings in this study were as follows; First, there was a relationship between the cooperative organizational culture of early childhood teachers and the overall type of conflict management. The overall cooperative organizational culture had the strongest relationship, particularly with the integrated type of conflict management. The following appeared in the order of compromise, consideration, and domination. On the other hand, there was no relationship with the avoidance type. Second, regarding the sub-factor of conflict management type that affects the cooperative organizational culture of early childhood teachers, the integrated type has the strongest influence, followed by the caring type. In the sub-factor of cooperative organizational culture, the integrated type has the strongest influence on the cooperative leadership, followed by the caring type. The integrated type had the greatest influence on teacher cooperation, peer support, and learning companion.

• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.79-86
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• 2022

Reproductive disorders in cows cause economic loss in livestock farms. This study was carried out to investigate the incidence of endometriosis in the uterine of Korean indigenous cow (Hanwoo). In the present study, the uterine of 25 cows was provided by the slaughterhouse. As a result on a visual examination of the uterus, 18 out of 25 were visually normal, and 7 uteruses (28%) appeared rather pale and showed purulent or mucosal symptoms in Uterine horn. However, the results of hematological analysis showed that both RBC and WBC were normal and showed no signs of systemic inflammation, indicating 7 cows showed asymptomatic endometriosis. The inflammatory uterus (28%) showed a wide range of pathological conditions that elicit an inflammatory response, such as serous exudate and bleeding. Histological and microscopic analysis in the inflammatory group demonstrated that there was swelling of the uterine glands, and neutrophil, basophil, and lymphocyte appeared in the uterine gland. Moreover, plasma cells and hemosiderin-laden macrophages were increased in the endometrial stroma, which lead to inhibit pregnancy by suppression of the synthesis of pregnancy hormones, and the appearance of hemosiderin-laden macrophages is an indicator of intracellular bleeding. In summary, hematologically, it is a normal diagnosis in Korean indigenous cows, however, when the uterus was extracted and investigated microscopically, the asymptomatic endometriosis were evident. In order to achieve the goal of healthy cow management and breeding within 2 weeks after birth, cows' uterus should be washed, disinfected, and through thorough the hygiene management, it aims to prevent asymptomatic endometriosis to produce healthy offspring and reduce the breeding interval.

• Journal of the Korean Institute of Landscape Architecture
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• v.50 no.4
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• pp.53-62
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• 2022

Animal companionship is gaining popularity in Korea, and interest in dog walking is also increasing as a strategy to maintain the physical and mental health of dogs and dog owners. The purpose of this study was to investigate the perception and satisfaction of dog owners with the neighborhood environments for dog walking and to examine the willingness to pay (WTP) for an off-leash dog park. Of a total of 249 online survey respondents, 47.8% said they take a walk at least once a day and mainly walk around nearby neighborhoods or parks. Respondents showed a high attachment to their dogs and recognized that dog walks help increase physical activity and maintain the health of owners and their dogs. The satisfaction with neighborhood environments, such as attractive views and the connection of walking trails, was low, and there was a high demand for off-leash dog parks. The truncated mean WTP was estimated to be 85,618 Korean Won, and the result of regression analysis and t-test revealed that walking frequency and the satisfaction with dog walking facilities were statistically significant in the WTP. In order to encourage dog walking to promote physical activity and health, more attention needs to be devoted to improving neighborhood environments for dog walking.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.1
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• pp.16-28
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• 2023

Lung adenocarcinoma accounts for about 40% of all lung cancers. With the recent development of gene profiling technology, studies on mutations in oncogenes and tumor suppressor genes, which are important for the development and growth of tumors, have been actively conducted. Companion diagnosis using next-generation sequencing helps improve survival with targeted therapy. In this study, formalin-fixed paraffin-embedded tissues of non-small cell lung cancer patients were subjected to hematoxylin and eosin staining for detecting genetic mutations that induce lung adenocarcinoma in Koreans. Immunohistochemical staining was also performed to accurately classify lung adenocarcinoma tissues. Based on the results, next-generation sequencing was applied to analyze the types and patterns of genetic mutations, and the association with smoking was established as the most representative cause of lung cancer. Results of next-generation sequencing analysis confirmed the single nucleotide variations, copy number variations, and gene rearrangements. In order to validate the reliability of next-generation sequencing, we additionally performed the existing genetic testing methods (polymerase chain reaction-epidermal growth factor receptor, immunohistochemistry-anaplastic lymphoma kinase (D5F3), and fluorescence in situ hybridiation-receptor tyrosine kinase 1 tests) to confirm the concordance rates with the next-generation sequencing test results. This study demonstrates that next-generation sequencing of lung adenocarcinoma patients simultaneously identifies mutation.