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QTL analysis of agronomic traits in recombinant inbred lines of sunflower under partial irrigation

  • Haddadi, P.;Yazdi-Samadi, B.;Naghavi, M.R.;Kalantari, A.;Maury, P.;Sarrafi, A.
    • Plant Biotechnology Reports
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    • v.5 no.2
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    • pp.135-146
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    • 2011
  • The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance ($R^2$) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance ($R^2$). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

A Development of Generalized Coupled Markov Chain Model for Stochastic Prediction on Two-Dimensional Space (수정 연쇄 말콥체인을 이용한 2차원 공간의 추계론적 예측기법의 개발)

  • Park Eun-Gyu
    • Journal of Soil and Groundwater Environment
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    • v.10 no.5
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    • pp.52-60
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    • 2005
  • The conceptual model of under-sampled study area will include a great amount of uncertainty. In this study, we investigate the applicability of Markov chain model in a spatial domain as a tool for minimizing the uncertainty arose from the lack of data. A new formulation is developed to generalize the previous two-dimensional coupled Markov chain model, which has more versatility to fit any computational sequence. Furthermore, the computational algorithm is improved to utilize more conditioning information and reduce the artifacts, such as the artificial parcel inclination, caused by sequential computation. A generalized 20 coupled Markov chain (GCMC) is tested through applying a hypothetical soil map to evaluate the appropriateness as a substituting model for conventional geostatistical models. Comparing to sequential indicator model (SIS), the simulation results from GCMC shows lower entropy at the boundaries of indicators which is closer to real soil maps. For under-sampled indicators, however, GCMC under-estimates the presence of the indicators, which is a common aspect of all other geostatistical models. To improve this under-estimation, further study on data fusion (or assimilation) inclusion in the GCMC is required.

Slope Stability by Variation of Rainfall Characteristic for Long Period (장기간 강우특성 변화에 따른 국내 사면의 안정성)

  • Lee, Jeong-Ju;Kim, Jae-Hong;Hwang, Young-Cheol
    • Journal of the Korean Geotechnical Society
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    • v.30 no.6
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    • pp.51-59
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    • 2014
  • Shallow landslides and debris flows are a common form of soil slope instability in South Korea. These events may be generally initiated as a result of intense rainfall or lengthening rainfall duration because of the effects of climate change. This paper presents the evaluation of rainfall-induced natural soil slope stability and reinforced soil slope instability under vertical load (railway or highway load) throughout South Korea based on quantitative analysis obtained from 58 sites rainfall observatories for 38 years. The slope stability was performed for infinite and geogrid-reinforced soil slopes by taking an average of maximum rainfall every ten years from 1973 to 2010. Seepage analysis is carried out on unsaturated soil slope using the maximum rainfall at each site, and then the factor of safety was calculated by coupled analysis using saturated and unsaturated strength parameters. The contour map of South Korea shows four stages in 10-year-time for the degree of landslide hazard. The safety factor map based on long term observational data will help prevent rainfall-induced soil slope instability for appropriate design of geotechnical structures regarding disaster protection.

Consortium Configuration of Local Governments for Disaster Joint Response Plan (재난 공동 대응을 위한 지자체 컨소시엄 구성 방안)

  • Jung, Woo Young;Lee, Chang Hee;Kim, Gi Hong
    • Journal of Korean Society for Geospatial Information Science
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    • v.21 no.2
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    • pp.71-83
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    • 2013
  • Recently frequencies and scales in natural disasters have been significantly increased and enlarged and that leads to increase interests on preventing disasters. As natural disasters occur, it is difficult to fulfill the scale of natural disasters using ordinary stocks in a local government. Thus, it is necessary to establish a system that commonly uses protection resources according to regional characteristics in order to rapidly implement labor mobilization and equipment distribution based on the damage of natural disasters. In this study, regional characteristics in Korea provinces are analyzed and 'a way of composing a local government consortium for common disaster responses' is proposed. First, a disaster map that represents the amount of damage for each local government published in the disaster yearbook from 2002 to 2011 is produced using GIS. Then, the whole land is divided into five different sections according to administrative regions and that are divided into 12 clusters again based on the geographical and humanistic characteristics and the analysis of the scale of damage in typhoons and heavy rainfalls. Finally, disaster protection base stations for each region are selected. The data of analyzing disaster damages may be used as a reference material for establishing disaster prevention plans.

A study on the potential market conditions and the road map of Korean vehicles for people with disabilities (장애인 복지차 시장현황과 한국형 장애인 복지차 로드맵에 관한 연구)

  • Rhee, K.M.;Kim, D.O.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.7 no.1
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    • pp.29-37
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    • 2013
  • The purpose of this study is to suggest the potential domestic and international market conditions vehicles for people with disabilities through a review of the literature and internet resources, and to propose a road map developing South Korean vehicles for people with disabilities taking into consideration the current state of domestic and international technology and the usage of the vehicles for people with disabilities depending on the type of disability. The results of the study are as follows: First, vehicles for people with disabilities safety standards of South Korea do not meet the safety standards of developed countries and the technology of development related vehicles for people with disabilities is not as advanced as that of developed countries. Vehicles for people with disabilities installation methods, specifications, features and safety standards should be designed to be more similar to those of Japan and the United States. Second, vehicle modifications for people with disabilities will become more common as measures directed at promoting the welfare of people with disabilities, such as travel and transportation measures, employment opportunities, education, and funding for vehicle modifications, are implemented along with laws, such as The Americans with Disabilities Act, The British Disability Discrimination Act, and Japan's barrier-free transport law, that protect people with disabilities generally. Third, through a comparative analysis of domestic and international market conditions and demands of people with disabilities in vehicle modifications, important things to pay attention by purchasing a modified vehicle for people with disabilities are the employment rate for people with disabilities and monthly income. To enable this, government agencies and industry bodies should work together through the revision of the current Disability Welfare Act or the enactment of the special law for guiding principles of Disability Welfare Act. Through the future changes in vehicles for people with disabilities-related system or society of South Korean, the annual demand of the vehicles is expected to be more than 10,000.

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Open GIS Component Software Ensuring an Interoperability of Spatial Information (공간정보 상호운용성 지원을 위한 컴포넌트 기반의 개방형 GIS 소프트웨어)

  • Choe, Hye-Ok;Kim, Gwang-Su;Lee, Jong-Hun
    • The KIPS Transactions:PartD
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    • v.8D no.6
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    • pp.657-664
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    • 2001
  • The Information Technology has progressed to the open architecture, component, and multimedia services under Internet, ensuring interoperability, reusability, and realtime. The GIS is a system processing geo-spatial information such as natural resources, buildings, roads, and many kinds of facilities in the earth. The spatial information featured by complexity and diversity requires interoperability and reusability of pre-built databases under open architecture. This paper is for the development of component based open GIS Software. The goal of the open GIS component software is a middleware of GIS combining technology of open architecture and component ensuring interoperability of spatial information and reusability of elementary pieces of GIS software. The open GIS component conforms to the distributed open architecture for spatial information proposed by OGC (Open GIS Consortium). The system consists of data provider components, kernel (MapBase) components, clearinghouse components and five kinds of GIS application of local governments. The data provider component places a unique OLE DB interface to connect and access diverse data sources independent of their formats and locations. The MapBase component supports core and common technology of GIS feasible for various applications. The clearinghouse component provides functionality about discovery and access of spatial information under Internet. The system is implemented using ATL/COM and Visual C++ under MicroSoft's Windows environment and consisted of more than 20 components. As we made case study for KSDI (Korea Spatial Data Infrastructure) sharing spatial information between local governments, the advantage of component based open GIS software was proved. Now, we are undertaking another case study for sharing seven kinds of underground facilities using the open GIS component software.

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Expression Analysis of Programmed Cell Death Genes in Porcine Parthenogenesis (돼지 단위생식란의 세포사멸 유전자 발현 양상에 관한 연구)

  • Son, Jong-Yoon;Kim, Sang-Hwan;Jung, Duk-Won;Ryu, Chun-Yeol;Yoon, Jong-Taek
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.239-248
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    • 2015
  • The nature of molecular mechanisms governing embryonic cell block is largely unknown, but recent reports have demonstrated that proper execution of programmed cell death is crucial for this process. The main objective of this study is to determine effects of programmed cell death on porcine oocytes development in vitro after parthenogenesis. Among the blastocysts matured in 3MA, MAP1LC3A and ATG5 RNA gene expression level increased in the order of Cyst < 3MA < RP. However, Casp-3 and TNF-r RNA gene expression level decreased in the order of RP < 3MA < Cyst. Expression of mTOR within the RP-cultured blastocyst was the most highly to the inner cell mass, while 3MA-cultured blastocyst showed very lowest expression in inner cell mass. The expression of mTOR showed a pattern opposite to that of MAP1LC3A. That is, its expression was the lowest in Cyst group. When the enzymatic activity of MMP-2 and MMP-9 was assessed in culture, the level of active MMP-9 was higher expression in the medium of each RP treatment group, with the level of another treatment group being relatively higher. Analyses of TIMP-2 and TIMP-3 revealed that their expression was higher in groups that did not receive RP treatment. More specifically, the level of TIMP-2 was not affected by Cyst treatment, while the level of TIMP-3 was higher in 3MA and RP treatment group. There was highly cell division activation efficiency of parthenogenesis on cultured system of RP supplement IVC medium. Therefore, these results suggest that embryo development was significantly increased in conditional culture medium with active autophagy as compared to common cultured condition. Further investigation of this distinction may enable the development of innovative improvements for the production of porcine somatic cell nuclear transfer.

Reference Station of Aerial Photogrammetry with GPS/INS by VRS (GPS/INS 항공사진측량의 지상기준국 측량의 VRS(가상기준점)적용에 관한 연구)

  • Song, Tae-Min;Kwon, Jay-Hyoun
    • Korean Journal of Remote Sensing
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    • v.24 no.4
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    • pp.381-388
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    • 2008
  • In the common practice of air-photographing measurement aerial-photogrammetric, the location of camera at the time of photographing is identified by performing aerotriangulation. However, installing ground-base station to enable aerotriangulation takes majority portion of a map making cost. Aerial-photogrammetric has shown a great improvement helped by steady upgrading in equipment and development in quantitative study. Aerotriangulation can be replaced by Direct Georeferencing, which uses GPS/INS to identify a camera location and to produce detailed information. An innovative technique replacing aerotriangulation, it has a disadvantage that base station has to be available in the area of photographing. The study intends to suggest a method applying VRS in GPS/INS aerotriangulation. Despite the fact that Direct Georeferencing is the innovated technique which substitutes existing aerotriangulation, it still need to install the ground-base station in GPS/INS aerotriangulation. GPS/INS data was analyzed with 4 different cases in order to accomplish the purpose of this study. In addition, in the thesis, it was approved that VRS can be utilized to make small-scale map as accurate as base station. This study is expected to improve the efficiency of work by showing that VRS can be used not only in base station but also enabling base station in the ground-access challenging area.

Landslide Susceptibility Assessment Using TPI-Slope Combination (TPI와 경사도 조합을 이용한 산사태 위험도 평가)

  • Lee, Han Na;Kim, Gihong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.36 no.6
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    • pp.507-514
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    • 2018
  • TSI (TPI-Slope Index) which is the combination of TPI (Topographic Position Index) and slope was newly proposed for landslide and applied to a landslide susceptibility model. To do this, we first compared the TPIs with various scale factors and found that TPI350 was the best fit for the study area. TPI350 was combined with slope to create TSI. TSI was evaluated using logistic regression. The evaluation showed that TSI can be used as a landslide factor. Then a logistic regression model was developed to assess the landslide susceptibility by adding other topographic factors, geological factors, and forestial factors. For this, landslide-related factors that can be extracted from DEM (Digital Elevation Model), soil map, and forest type map were collected. We checked these factors and excluded those that were highly correlated with other factors or not significant. After these processes, 8 factors of TSI, elevation, slope length, slope aspect, effective soil depth, tree age, tree density, and tree type were selected to be entered into the regression analysis as independent variables. Three models through three variable selection methods of forward selection, backward elimination, and enter method were built and evaluated. Selected variables in the three models were slightly different, but in common, effective soil depth, tree density, and TSI was most significant.

Ginsenoside Rg1 attenuates cerebral ischemia-reperfusion injury due to inhibition of NOX2-mediated calcium homeostasis dysregulation in mice

  • Han, Yuli;Li, Xuewang;Yang, Liu;Zhang, Duoduo;Li, Lan;Dong, Xianan;Li, Yan;Qun, Sen;Li, Weizu
    • Journal of Ginseng Research
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    • v.46 no.4
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    • pp.515-525
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    • 2022
  • Background: The incidence of ischemic cerebrovascular disease is increasing in recent years and has been one of the leading causes of neurological dysfunction and death. Ginsenoside Rg1 has been found to protect against neuronal damage in many neurodegenerative diseases. However, the effect and mechanism by which Rg1 protects against cerebral ischemia-reperfusion injury (CIRI) are not fully understood. Here, we report the neuroprotective effects of Rg1 treatment on CIRI and its possible mechanisms in mice. Methods: A bilateral common carotid artery ligation was used to establish a chronic CIRI model in mice. HT22 cells were treated with Rg1 after OGD/R to study its effect on [Ca2+]i. The open-field test and poleclimbing experiment were used to detect behavioral injury. The laser speckle blood flowmeter was used to measure brain blood flow. The Nissl and H&E staining were used to examine the neuronal damage. The Western blotting was used to examine MAP2, PSD95, Tau, p-Tau, NOX2, PLC, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging was used to test the level of [Ca2+]i. Results: Rg1 treatment significantly improved cerebral blood flow, locomotion, and limb coordination, reduced ROS production, increased MAP2 and PSD95 expression, and decreased p-Tau, NOX2, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging results showed that Rg1 could inhibit calcium overload and resist the imbalance of calcium homeostasis after OGD/R in HT22 cells. Conclusion: Rg1 plays a neuroprotective role in attenuating CIRI by inhibiting oxidative stress, calcium overload, and neuroinflammation.