• Korean Chemical Engineering Research
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• 제56권4호
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• pp.524-535
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• 2018

본 연구에서는 에멀젼 자기조립틀을 활용하여 거대기공을 갖는 타이타니아 분말을 합성하였으며, 자외선 조사를 활용한 광촉매 실험을 통해 상용화된 타이타니아 나노입자와 다공질 타이타니아 분말을 이용한 고초균의 살균 효과를 비교하였다. 다공질 분말의 기공 크기를 달리하여 자외선 조사 시간에 따른 고초균 수의 감소를 측정하였으며, 살균 실험의 최적 조건을 도출하기 위하여 기공 크기에 따른 다공질 타이타니아 분말의 살균력을 비교하였다. 기공 크기가 작아질수록 살균 효과는 증대됨을 관찰하였으며, 광촉매의 활용 및 자외선 조사에 의해 1시간 동안 50% 이상의 고초균이 제거되는 효과를 확인하였다. 또한 활성 화학종의 발생을 촉진하기 위하여 과산화수소의 희석 용액을 광촉매 살균 방식과 결합하였으며, 1시간 동안의 자외선 조사에 의해 균주의 거의 대부분이 사멸되는 등 고초균에 대한 살균 효과를 증진시킬 수 있었다.

• 한국환경과학회지
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• 제16권2호
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• pp.219-225
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• 2007

Endocrine disrupting compounds (EDC's) are chemicals that either mimic endogenous hormones interfering with pharmacokinetics or act by other mechanisms. Some endocrine disrupters were reported to be chemical substances that cause apoptosis in cells. A number of reports have indicated that 1,3-DCP, one of the EDC's may act as an endocrine disrupter and also has possible carcinogenic effects. 1,3-DCP, present in commercial protein hydrolysates used for human nutrition, are genotoxic and 1,3-dichloro-2-propanol induced tumors in rats. In the present study, it was investigated whether 1,3-DCP induces ROS generation and apotosis in A549 adenocarcinoma cells. Here we show that 1,3-DCP inhibits the growth of lung cancer cell lines and generates reactive oxygen species (ROS), a major cause of DNA damage and genetic instability, It was investigated that 1,3-DCP increases G1 phase cells after 12 hours, thereafter abruptly draws A549 cells to G0 state after 24 hours by flow cytometric analysis. 1,3-DCP induces p53 and $p21^{Cip1/WAF1}$ activation time- and dose-dependently by 24 hours, while the level $p21^{Cip1/WAF1}$ was decreased after 48 hours. These results suggest that 1,3-DCP, an EDC's generates ROS and regulates genes involved with cell cycle and apoptosis.

• 한국환경보건학회지
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• 제46권5호
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• pp.513-524
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• 2020

Objective: The main purpose of this study is to evaluate the environmental and biological exposure of local residents who consumed arsenic-contaminated drinking water for less than one year. Methods: As a part of water quality inspections for small-scale water supply facilities, surveys were conducted of residents of two villages that exceeded the arsenic threshold for drinking water. The environmental impact survey consisted of surveys on water quality, soil, and crops in the surveyed area. Biological monitoring was performed by measuring the separation of arsenic species in urine and total arsenic in hair. Results: In the results of biological monitoring, the concentrations of AsIII and AsV were 0.08 and 0.16 ㎍/L, respectively. MMA and DMA were 0.87 and 36.19 ㎍/L. There was no statistically significant difference between the group who drank arsenic-removed groundwater or water from the small-scale supply facility and the group who drank tap water, purified water, or commercial bottled water. Some of the water samples exceeded the arsenic threshold for drinking water. There were no samples in the soil or rice that exceeded the acceptable threshold. Conclusion: In the case of short-term exposure to arsenic-contaminated drinking water for less than one year, there were no significant problems of concern from the evaluation of biological monitoring after arsenic was removed.

• 에너지공학
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• 제12권4호
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• pp.320-327
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• 2003

IGCC용 가스터빈 연소기의 중발열량 가스 연료 대체성 및 호환성 검토를 위한 전산유체역학적 연구를 수행하였다. 연소기 전산해석 방법은 기존의 상용 CFD코드의 해석체계에 중발열량 가스연료의 화학반응 모델 및 fuel NOx 모델등을 추가적으로 결합하여 구성하였다. 본 해석방법을 이용하여, 천연가스와 IGCC 용 대체가스(석탄가스, 중잔사유 가스) 연소시의 연소기 내부 유동속도, 화학종, 온도 분포들과 화염 형상 및 거동을 비교하였고, 더 나아가 NOx 생성특성과 터빈과의 matching 조건도 분석하였다. 이러한 전산해석결과들을 바탕으로, 본 연구는 중발열량 가스를 대체연료로 사용하는 IGCC용 가스터빈 연소기의 설계 개선 및 재설계 방향을 제시하였다.

• 한국발생생물학회지:발생과생식
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• 제17권3호
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• pp.231-240
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• 2013

Olive flounder (Paralichthys olivaceus) is one of the commercial important flatfish species in Korea. The ocular signal transduction pathway is important in newly hatched flounders because it is closely involved in the initial feeding phase thus essential for survival during the juvenile period. However, the study of gene expression during ocular development is incomplete in olive flounder. Therefore we examined the expression analysis of specifically induced genes during the development of the visual system in newly hatched flounders. We searched ocular development-involved gene in the database of expressed sequence tags (ESTs) from olive flounder eye and this gene similar to arrestin with a partial sequence homology. Microscopic observation of retinal formation corresponded with the time of expression of the arrestin gene in the developmental stage. These results suggest that arrestin plays a vital role in the visual signal transduction pathway of the retina during ocular development. The expression of arrestin was strong in the ocular system during the entirety of the development stages. Our findings regarding arrestin have important implications with respect to its biological role and evolution of G-protein coupled receptor (GPCR) signaling in olive flounder. Further studies are required on the GPCR-mediated signaling pathway and to decipher the functional role of arrestin.

• 원예과학기술지
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• 제34권2호
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• pp.242-248
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• 2016

This research was conducted to determine the effects of four different substrates, expanded rice hulls (ERH), commercial substrates for strawberries (CSS), clay sand (CS), and loamy sand (LS), on the inhibition of anthracnose crown rot (ACR) in strawberry. Mother plants of 'Seolhyang' strawberry were transplanted into an elevated bed in March, 2013 and March, 2014 and the runners connecting mother plants and daughter plants were cut in early August of both years. After separation, growth characteristics of the daughter plants were measured and then each daughter plant was inoculated with conidial suspensions of Colletotrichum fructicola, one of several species of Colletotrichum that causes ACR in strawberries. The incidence of ACR as influenced by the different substrates was investigated in both years. The daughter plants grown on CSS had the highest values for shoot height, leaf area, and fresh weight. Those grown on ERH and LS substrates also displayed good above-ground growth characteristics except for fresh weight, but the daughter plants grown on CS had the poorest above-ground growth characteristics. The ERH and CS treatments resulted in the highest number of primary roots and the greatest root weight. The CSS-grown daughter plants had the highest ACR disease index, followed by the CS and LS treatments, but there were no significant differences among the three substrates. However, the ERH-grown daughter plants had a markedly lower ACR disease index on October 11, 2013 and October 7, 2014. The CSS-grown daughter plants had high nitrogen and potassium contents and low calcium content, whereas the ERH-grown daughter plants had low nitrogen levels and high silicon levels. The results of this study provide basic information on the ability of the different substrates tested to provide disease suppression of ACR in the propagation of strawberry transplants.

• 식물병연구
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• 제10권4호
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• pp.260-267
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• 2004

2001년부터 2003년에 걸쳐 수확 후 과일가게에서 판매되는 참외의 썩음증상으로부터 Fusarium spp.를 분리하였다. 병든 과실들은 균사로 덮여 있었고 결국 물러 썩었다. 병의 시작은 주로 과실이 달렸던 부위, 꽃이 달렸던 부위 그리고 열매 표면에 병이 시작되었다. 병이 진전됨에 따라 물러 썩는 열매의 표면에는 흰색에서 분홍빛의 균사로 덮혔다. 이들 병든 과실로부터 분리된 Fusarium spp.를 기존에 보고된 균학적 내용과 비교하여 동정한 결과 Fusarium equiseti, F. graminearum, F. monliforme, F. proliferatum, F. sambucinum, F. semitectum으로 동정되었다. 이들 균의 병원성을 조사하기 위해 건전한 참외에 인위적으로 만든 상처와 무상처에 접종한 결과 접종 이틀후, 상처 접종 부위에 기중균사가 형성되었고, 병이 진전됨에 따라 물러 썩었다. 비록 참외에서 Fusarium spp.가 야기하는 열매썩음병은 국내에서 이미 보고된 바가 있으나, 이와 관련된 종에 대해서는 보고된 적이 없다. 따라서, 이 연구를 통해 Fusarium spp.에 의한 수확 후 참외의 열매썩음병을 국내에서 처음으로 보고하는 바이다.

• The Plant Pathology Journal
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• 제33권2호
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• pp.140-147
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• 2017

Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information from F. oxysporum f. sp. fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed using F. oxysporum f. sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-${\alpha}$, and ${\beta}$-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the ${\beta}$-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.207-212
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• 2007

식물현탁배양세포의 whole cell extract의 $^1H$ NMR 스펙트럼 데이터로부터 통계분석기법을 활용하여 GABA의 간단하고 신속한 정량분석방법을 확립하였다. 이 기술을 활용하여 고등식물 8종의 9개 세포주를 MS 배지에 1 mg/L의 2,4-D를 첨가한 배지에 유지하였을 때 고수 (Coriandrum sativum L.)가 가장 많은 양의 GABA를 생산하였다. 고수 현탁배양세포로부터 2,4-D농도 및 배양기간에 따른 GABA의 생산성 변화를 조사한 결과 현탁배양세포를 0.5 mg/L 2,4-D가 첨가된 배지에서 3주간 배양된 현탁배양세포를 이용할 경우 GABA 함량이 건중량 1 g 당 16.9 mg으로 가장 높게 생산되었다. 본 연구에서 확립된 간단하고 신속한 분석법으로 다양한 식물자원으로부터 GABA의 생산성을 초고속탐색(high-throughput screenig)할 수 있을 것이며 고수 현탁세포배양법으로 GABA의 상업적 대량생산이 가능할 것으로 전망된다.

• 미생물학회지
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• 제46권1호
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• pp.109-111
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• 2010

다환 방향족 탄화수소(PAH; polycyclic aromatic hydrocarbon)는 발암성, 돌연변이 유발성, 유전독성을 지니기 때문에 인체위해성이 큰 물질로 알려져 있다. 기존 PAH 분해 미생물 탐색 방법중 독성이 강한 유기용매에 PAH를 용해시켜 미생물에 직접 분무하는 분무법, 미생물과 직접 혼합하는 한천중층법은 미생물 생장에 영향을 줄 뿐만 아니라, 특히 많이 쓰이는 분무법의 경우 분무되는 PAH의 양을 조절하기가 어렵고 멸균상태를 유지하기가 힘들다는 단점이 있다. 그래서 본 논문에서는 이와 같은 단점을 보완한 방법으로 승화법(Alley, Jeremy F. and Lewis R. Brown. 2000. Use of sublimation to prepare solid microbial media with water-insoluble substrates. Appl. Environ. Microbiol. 66, 439-442)을 도입하여 적용하였다. 그 결과 상용휘발유 및 태안유류유출지 시료로부터 분리한 350분리균주 중에서 7균주가 단일 PAH 또는 복수의 PAH 분해에 관여했다. 특히 Corynebacterium sp. SK20, Rhodococcus sp. TA24, Streptomyces sp. TA27은 시험한 pyene, phenanthrene, naphthalene에, Gordonia sp. H37는 pyrene, naphthalene에, Arthrobacter sp. S49는, naphthalene, phenanthrene에 활성이 있었다.