• 한국식품영양과학회지
• /
• 제31권4호
• /
• pp.594-598
• /
• 2002

과일의 방사선 조사 여부 확인과 저장에 따른 변화를 측정하기 위해 감마선 조사로 유도된 DNA손상을 comet assay로 확인하였다. 키위, 오렌지, 배를 구입하여 0.1, 0.3, 0.5, 0.7, 1.0 kGy의 저 선량으로 조사하고 비 조사 시료와 조사 시료간의 DNA 손상정도를 tail length와 tail moment로 측정하였다. 과일 씨의 DNA를 형광 염색하여 이미지 분석기를 이용하여 comet 양상을 관찰한 결과, 모든 시료에서 비 조사 시료보다 조사시료의 tail length가 더 길었으며 조사 선량이 증가할수록 tail length가 유의적으로 길게 나타났다. Tail moment로 나타난 결과도 이와 비슷하였으나 전체적으로 tail length에 비해 그 민감도가 낮았다. 방사선 조사한 과일을 저온에서 3개월 동안 저장한 후에도 저장 전과 마찬가지로 모든 시료에서 비 조사시료보다 조사 시료의 tail length가 더 길었으며, 조사 선량이 증가할수록 tail length가 길게 나타나, 저장 후에도 comet assay를 이용해 조사 시료와 조사 시료의 방사선 조사여부를 검지할 수 있었다 따라서 본 연구결과 comet assay는 신선한 과일과 일정기간 저장한 과일의 방사선 조사여부 판별에 유용하게 사용될 수 있음을 알 수 있었다.

• Toxicological Research
• /
• 제20권2호
• /
• pp.89-100
• /
• 2004

The comet assay (also called the single-cell gel electrophoresis assay) has been widely used for detecting DNA damage and repair in individual cells. Since the conventional methods of evaluating comet assay data using frequency statistics are unsatisfactory we developed a new quantitative measure of DNA damage/repair that is based on all information residing in the dose/time-response curves of a comet experiment. Blood samples were taken from 25 breast cancer patients before undergoing radiotherapy. The comet assay was performed under alkaline conditions using isolated lymphocytes. Tail DNA, tail length, tail moment and tail inertia of the comet were measured for each patient at four doses of $\gamma$-rays (0, 2, 4 and 8 Gy) and at four time points after irradiation (0, 10, 20 and 30 min) using 100 cells each. The resulting three-dimensional dose-time response surface was modeled by multiple regression, and the second derivative, termed 2D, on dose and time was determined. A software module was programmed in SAS/AF to compute 2D values. We applied the new method successfully to data obtained from cancer patients to be assessed for their radiation sensitivity. We computed the 2D values for the four damage measures, i.e., tail moment, tail length, tail DNA and tail inertia, and examined the pairwise correlation coefficients of 2D both on the log scale and the unlogged scale. 2D values based on tail moment and tail DNA showed a high correlation and, therefore, these two damage measures can be used interchangeably as far as DNA repair is concerned. 2D values based on tail inertia have a correlation profile different from the other 2D values which may reflect different facets of DNA damage/repair. Using the dose-time response surface, other statistical models, e.g., the proportional hazards model, become applicable for data analysis. The 2D approach can be applied to all DNA repair measures, Le., tail moment, tail length, tail DNA and tail inertia, and appears to be superior to conventional evaluation methods as it integrates all data of the dose/time-response curves of a comet assay.

• 한국환경성돌연변이발암원학회:학술대회논문집
• /
• 한국환경성돌연변이발암원학회 2003년도 추계학술대회
• /
• pp.133-133
• /
• 2003

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2001년도 International Symposium on Signal transduction in Toxicology
• /
• pp.116-116
• /
• 2001

The alkaline Comet assay has been used with increasing popularity to investigate the level of DNA damage in terms of strand breaks and alkaline labile sites in biomonitoring studies within the last decade in western countries.(omitted)

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2003년도 추계학술대회
• /
• pp.95-95
• /
• 2003

The comet assay has been recently validated as a sensitive and specific test system for the quantification of DNA damage. with 11 substances that demonstrated positive results in at least one test among 4 standard short-term genotoxicity tests, and to evaluate its ability to predict rodent carcinogenicity.(omitted)

• Journal of Astronomy and Space Sciences
• /
• 제17권2호
• /
• pp.199-210
• /
• 2000

이 연구는 1997년 2월 21일부터 5월 l일까지 소백산천문대 61 cm망원경과 CCD카메라, 그리고 5개의 협대역 필터를 이용하여 근일점 근방을 지나는 헤일-밥 혜성 (Comet Hale-Bopp, C/1995 01) 코어 4’ 내의 CN, $C_2$, $C_3$ 방출선에 대한 분광측광 관측 결과이다. 이 연구에서는 협대역 필터를 이용한 분자 방출선 영상의 형태학적인 특징과 근일점 근처에서의 시간에 따른 분자 방출 선의 변화 추이에 대하여 논의하였다. 헤일-밥 혜성은 형태학적인 면에서 중심에서 반경 4' 내에 있는 혜성 코어의 CN의 분자 방출선은$C_2$나 $C_3$에 비하여 구형의 대청척인 형태를 보인 반면, $C_2$나 $C_3$ 분자의 방출선은 비대청적인 분포를 보였다. 그리고 이들 분자의 방출선 영상에서 광대역 필터의 영상에 나타난 나선팔과 같은 형태학적 특성이 나타났다. 한편 측광학적인 특정으로 $C_2$, $C_3$ 분자 방출선의 광도 분포는 CN 보다 중심 집중도가 높게 나타났으며, 태양으로부터 거리에 따른 CN의 표면광도 변화는 $C_2$, $C_3$ 표면광도 변화와 약간 다른 경향을 나타내었다. 그 러나 근일점 근처에서 이들 분자의 방출선의 광도의 따른 변화는 이천 연구에서 제기되었던 7일 주기로 나타나는 극대 광도를 보여 주고 있다.

• 한국환경성돌연변이발암원학회지
• /
• 제21권1호
• /
• pp.34-43
• /
• 2001

Di-2-ethylhexyl phthalate (DEHP) is the most commonly used phthalate ester in polyvinyl chloride formulations including food packing and storage of human blood. DEHP is a well known as non-genotoxic carcinogen and endocrine disrupting chemical (EDC). DEHP have shown all negative results in ICH-guildeline recommended standard genotoxicity test battery. In this study, to assess the clastogenic and DNA damaging effect in human-derived tissue specific cells, DEHP was treated in human derived MCE-7 cells, HepG2 cells, LNCap cells, BeWo cells, MCE-10A cells, and female peripheral blood cells using micronucleus assay and in human breast carcinoma MCF-7 cells up to $1.28$\times$10^{-2}$ M using Comet assay. The in vitro micronucleus assay is a mutagenicity test system for the detection of chemicals which induce the formation of small membrane bound DNA fragment i.e. micronuclei in the cytoplasm of interphase cells, originated from clastogenic and/or aneugenic mechanism. The single cell gel electrophoresis assay (Comet assay) is used to detect DNA strand-breaks and alkaline labile site. In our results, DEHP increased significantly and/or dose-depentently and time-dependently micronucleus frequency at the 6 and 24 hr without metabolic activation system only in MCE-7 cells. DEHP treated with 2 hrs in MCF-7 cells using Comet assay induced DNA damage dose-depentantly.

• 천문학회보
• /
• 제41권2호
• /
• pp.50.3-51
• /
• 2016

Recent analysis on the thermophysical property of asteroids revealed that their thermal inertia decrease with their sizes at least for main belt asteroids. However, little is known about that of comet-like bodies. In this work we utilized a simple thermophysical model (TPM) to calculate the thermal inertia of a bare nucleus of the comet P/2006 HR30 (Siding Spring) and an asteroid in comet-like orbit 107P/(4015) Wilson-Harrington from AKARI observation data. From five spectroscopic observations of the targets, we find out that the former has thermal inertia of around $2,000J\;m^{-2}K^{-1}s^{-1/2}$ (using pV = 0.055) and the latter has about $1,000-2000J\;m^{-2}K^{-1}s^{-1/2}$ (using pV = 0.055 and 0.043, respectively). These are high enough for both of them to deposit water ice at few centimeters depth, and hence it is difficult to say they are cometary based on the results of this study. These values, however, dependent significantly on the errors of observation and the uncertainties of the input parameters, as well as other conditions which are ignored in simple TPM approach, such as shape model and surface roughness. Further detailed analyses on these cometary bodies will shed light on our understanding of the detailed surfacial characteristics of them.

• 천문학회보
• /
• 제42권2호
• /
• pp.49.3-50
• /
• 2017

There have been recent studies which revealed a tendency that thermal inertia decreases with the size of asteroidal bodies, and suggestions that thermal inertias of cometary bodies should be much smaller than those asteroidal counterparts, regardless of comets' nuclear sizes, which hints a way to differentiate cometary candidates from asteroids using thermal inertia information. We thus selected two comet-like objects from AKARI satellite of JAXA, namely, 107P/ (4015) Wilson-Harrington and P/2006 HR30 (Siding Spring), and applied simple thermophysical model to test the idea. Both targets did not show any comet-like activity during the observations. From the model, we found Wilson-Harrington to have size of 3.7-4.4 km, geometric albedo 0.040-0.055 and thermal inertia of 100-250 J m-2 K-1 s-0.5, which coincide with previous works, and HR30 to have size of 24-27 km, geomoetric albedo of 0.035-0.045 with thermal inertia of 250-1000 J m-2 K-1 s-0.5. HR30 is found to have the rotation pole near the ecliptic plane (the latitude between -20 and +60 deg). Based on the results, we conjecture that comet-like objects are not clearly distinguishable from asteroidal counterpart using thermal inertia.

• 한국환경농학회지
• /
• 제34권4호
• /
• pp.350-354
• /
• 2015

BACKGROUND: Botanical insecticides, especially Azadirachta Indica extract (AIE) and Sophorae radix extract (SRE) are widely used in Agriculture field. In our previous studies on genotoxicity test of AIE and SRE samples, a suspicious clastogenic properties was shown. Herein, we investigated the DNA damage effect of these botanical insecticide samples through the in vitro comet assay. METHODS AND RESULTS: Chinese hamster lung (CHL) fibroblast cell line was used, and methyl methanesulphonate was as positive control. Respective two samples of AIE and SRE were evaluated using Single Cell Gel Electrophoresis (Comet) assay and measured as the Olive tail moment (OTM). Results from this study indicated that all tested AIE and SRE samples did not show DNA damage in comet assay using CHL cells, compared with control. CONCLUSION: AIE and SRE samples used in this study were not cause genetic toxicity and are suitable for use as organic materials.