Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)
- Volume 21 Issue 1
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- Pages.34-43
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- 2001
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- 1225-6307(pISSN)
DNA Damage and Micronuclei Induced by Di (2-ethylhexyl) phthalate in Human Breast Carcinoma MCF-7 cells
Di(2-ethylhexyl) phthalate에 의해 유도된 DNA손상과 소핵 형성
Abstract
Di-2-ethylhexyl phthalate (DEHP) is the most commonly used phthalate ester in polyvinyl chloride formulations including food packing and storage of human blood. DEHP is a well known as non-genotoxic carcinogen and endocrine disrupting chemical (EDC). DEHP have shown all negative results in ICH-guildeline recommended standard genotoxicity test battery. In this study, to assess the clastogenic and DNA damaging effect in human-derived tissue specific cells, DEHP was treated in human derived MCE-7 cells, HepG2 cells, LNCap cells, BeWo cells, MCE-10A cells, and female peripheral blood cells using micronucleus assay and in human breast carcinoma MCF-7 cells up to
Keywords
- micronucleus assay;
- single cell gel electrophoresis assay (Comet assay);
- Di (2-ethylhexyl) phthalte;
- MCF-7 cells