• Journal of Applied Biological Chemistry
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• 제59권1호
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• pp.69-74
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• 2016

시금치에 fumaric acid와 mild heat의 병합처리를 통해 병원성 미생물 제어효과를 규명하고자 시금치에 E. coli O157:H7, L. monocytogenes 를 접종한 후 각 단일처리 후 미생물 수 변화를 측정하였다. Fumaric acid (0.1, 0.3, 0.5%)와 mild heat (40, 50, $60^{\circ}C$)의 각 단일처리 실험 결과를 토대로, 병합처리를 위한 fumaric acid의 최적농도는 0.5%, mild heat 처리조건으로 $50^{\circ}C$에서 5 min으로 선정하였고, 병합처리 시 L. monocytogenes, E. coli O157:H7의 수는 대조구에 비해 각각 2.53, 2.62 log CFU/g 감소하였다. 그리고 신선한 시금치에 병합처리 후 $4^{\circ}C$에서 12일간 저장하면서 미생물 수 감소 및 품질 변화를 조사하였다. 시금치의 초기 미생물 수에 있어서 대조구와 비교하여, 병합 처리구에서 총 호기성 균을 2.77 log CFU/g 감소시켰다. 특히, 저장 12일 후 병합 처리구의 총 호기성 균 수는 4.84 log CFU/g으로 대조구와 비교하여 1.82 log CFU/g의 감균 효과를 가졌다. 또한 시금치의 저장 중 Hunter 색도 값 및 비타민 C 함량에 있어서 처리구 간의 유의적인 차이를 보이지 않았다. 따라서 본 연구 결과, fumaric acid와 mild heat의 병합처리가 시금치의 미생물학적 안전성 유지에 효과적인 처리라고 판단된다.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.119-123
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• 1998

The feasibility of using combined treatments of electric field and chemical mutagen N-methyl-N'-nitro-N-nitroso-guanidine (NTG) for the strain improvement of Saccharomyces sp. in ethanol production was examined. The treatment of electric field alone resulted in no effect on the lethality of yeast cells under the conditions of this study. However, when the electric field was applied together to the treatment of yeast cells with NTG, the electric field increased the lethal effect and auxotrophic mutation rate of NTG. The combined treatment of electric field and NTG also increased the chances of. obtaining superior yeast strains for the ethanol production from tapioca. A higher number of improved clones was obtained by the combined treatments of electric field and NTG than by the NTG treatment alone. The best clone, NF 30-9, which was also obtained by the combined treatment, produced $11.07\%$ (w/v) ethanol from tapioca slurry containing 25% (w/v) reducing sugar while the parental strain produced 9.77% (w/v).

• 한국환경보건학회지
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• 제23권2호
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• pp.64-71
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• 1997

This study was performed to investigate the effect of co-existence of carbofuran and chlorothalonil on the short-term bioconcentration factor in Brachydanio rerio(zebrafish). The fishes were exposed to the single and combined treatment of carbofuran and chlorothalonil for 1, 3 and 5 days. Experimental concentrations of carbofuran were 0.05 and 0.10 ppm under the single treatment. And those of chlorothalonil were 0.005 and 0.010 ppm. Experimental concentrations of the combined treatment of carbofuran and chlorothalonil were 0.05 ppm+0.005 ppm, 0.05 ppm+0.010 ppm, 0.10 ppm+0.005 ppm for 1, 3 and 5 days, respectively. Carbofuran and chlorothalonil in fish and in test water were extracted with n-hexane and acetonitrile. GC-ECD was used to detect and quantitate carbofuran and chlorothalonil. 1-day, 3-day and 5-day bioconcentration factors(BCF$_1$, BCF$_3$ and BCF$_5$) of each pesticide were obtained from the quantitation results. The depuration rate of each pesticide was determined over the 24-h period after combined treatment. The results were as follows: Carbofuran did not bioaccumulate in zebrafish under the single and combined treatment for testing periods. BCF$_1$ values of chlorothalonil in concentration of 0.005 and 0.010 ppm under the single treatment were 0.508, 0.621, BCF$_3$ were 1.327, 1.511 and BCF$_5$ were 1.331, 1.597, respectively. BCF$_1$ values of chlorothalonil were 0.512, 0.520 and 0.619, respectively, when the concentration of carbofuran and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. BCF$_3$ values of chlorothalonil 1.341, 1.338 and 1.513, respectively, and BCF$_5$ values of chlorothalonil were 1.332, 1.327 and 1.521, respectively, under the above combined treatment. Depuration rate constants of chlorothalonil in concentration of 0.005 and 0.010 ppm under the single treatment were 0.011 and 0.012. Depuration rate constants of chlorothalonil were 0.011, 0.010 and 0.011, when the concentration of carbofuran and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. It was observed that no significant difference of carbofuran and chlorothalonil concentration in fish extracts, test water, BCFs and depuration rate constants of carbofuran and chlorothalonil between combined treatment and single treatment. It was considered that no appreciable interaction at experimental concentrations due to lower concentrations than LC$_{50}$. It is suggested that the difference of BCFs between carbofuran and chlorothalonil due to those of fat composition of fish and solubility of carbofuran and chlorothaionil.

• Archives of Plastic Surgery
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• 제44권3호
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• pp.194-201
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• 2017

Background Platelet-rich plasma (PRP) contains high concentrations of growth factors involved in wound healing. Hydrogel is a 3-dimensional, hydrophilic, high-molecular, reticular substance generally used as a dressing formulation to accelerate wound healing, and also used as a bio-applicable scaffold or vehicle. This study aimed to investigate the effects of PRP and hydrogel on wound healing, in combination and separately, in an animal wound model. Methods A total of 64 wounds, with 2 wounds on the back of each nude mouse, were classified into 4 groups: a control group, a hydrogel-only group, a PRP-only group, and a combined-treatment group. All mice were assessed for changes in wound size and photographed on scheduled dates. The number of blood vessels was measured in all specimens. Immunohistochemical staining was used for the analysis of vascular endothelial growth factor (VEGF) expression. Results Differences in the decrease and change in wound size in the combined-treatment group were more significant than those in the single-treatment groups on days 3, 5, 7, and 10. Analysis of the number of blood vessels through histological examination showed a pattern of increase over time that occurred in all groups, but the combined-treatment group exhibited the greatest increase on days 7 and 14. Immunohistochemical staining showed that VEGF expression in the combined-treatment group exhibited its highest value on day 7. Conclusions This experiment demonstrated improved wound healing using a PRP-hydrogel combined treatment compared to either treatment individually, resulting in a decrease in wound size and a shortening of the healing period.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7805-7809
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• 2014

Objective: To explore the clinical efficacy and prognostic factors of chemoreduction combined with topical treatment of advanced intraocular retinoblastoma (RB). Materials and Methods: A total of 22 eyes from 17 children with RB were selected for the study and treated with chemoreduction combined with topical cryotherapy, transpupillary thermotherapy (TTT) or episcleral plaque brachytherapy. Clinical and follow-up data were retrospectively analyzed. Results: All children received 2~6 courses of chemoreduction treatment, ($4.5{\pm}0.8$ courses on average); 17 eyes from 13 children were treated by chemoreduction combined with cryotherapy or TTT and 5 eyes from 4 children with chemoreduction combined with $^{125}I$ episcleral plaque brachytherapy. The eye retention rate was 81.8% (18/22), among which 38.9% (7/18) featured restored or maintained good vision. Postoperative follow-up period was 7 to 34 months, ($18.6{\pm}5.2$ months on average). The recurrence rate was 41.2% (7/17), among which 57.1% (4/7) were controlled by supplementing or appending cryotherapy or TTT treatment during the follow-up period. The tumor basal diameter and thickness were significantly reduced (P<0.05 or P<0.01) after treatment. All children demonstrated different degrees of hair loss, 70.6% (12/17) with different degrees of gastrointestinal reactions, 5.88% (1/17) with neutropenia and 11.8% (2/17) being seriously infected during the chemotherapeutic treatment. Univariate and logistic regression analysis showed that tumor basal diameter before treatment had a significant effect on the prognosis (P<0.01). Conclusions: Chemoreduction combined with topical therapy can effectively control RB in the short term, and tumor basal diameter before treatment is an independent risk factor for prognosis.

• 공업화학
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• 제29권5호
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• pp.613-619
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• 2018

본 연구에서는 유입 슬러지에 다양한 전처리 방법과 고액분리장치를 이용한 유출수의 잉여슬러지를 농축 후 내부반송을 적용하여, 중온혐기-고온호기 혼합 슬러지 처리 공정의 슬러지 소화효율과 메탄가스 생성량에 미치는 영향을 비교 검증하였다. 실험실 규모의 혼합 소화공정장치를 제작하여 서로 다른 유입 슬러지 전처리방법을 적용하여 5단계로 실험을 진행하였다. 1단계에서는 열-알칼리처리 전처리를 하여 슬러지를 공급하였고, 2, 3, 4단계에서는 유출수로부터 농축된 잉여슬러지의 내부반송과 각각 열-알칼리처리, 열처리, 알칼리 처리(7일)를 거친 유입 슬러지를 공급하였다. 마지막 5단계에서는 전처리를 하지 않은 슬러지를 공급하였다. 실험 결과, 1단계에서 4단계까지 진행되는 동안 Volatile Suspended Solid (VSS) 제거율은 유입 슬러지 전처리와 내부반송을 적용하는 경우 크게 증가하였으며, 메탄생성량 또한 2단계에서 슬러지 내부반송과 열-알칼리처리 전처리 적용의 경우 285 mL/L/day까지 크게 증가하였다. 한편, 5단계에서 전처리를 하지 않은 슬러지를 공급하였을 경우 VSS 제거율과 메탄 생성량이 크게 감소하였다. 결론적으로, 유입 슬러지의 열-알칼리처리 전처리와 유출수의 농축 잉여슬러지의 내부반송을 통해 복합 슬러지 처리 공정의 슬러지 제거 효율과 메탄생성량을 크게 증가시킬 수 있었다.

• 대한한의학방제학회지
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• 제25권3호
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• pp.391-412
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• 2017

Objective : In this study, we searched the experimental research about combined treatment of anticancer drug and herbal medicine for killing or inhibiting proliferation of Cancer cells searched in OASIS and KISS. This study aimed to analyze the experimental research paper about anticancer drug combined treatment with herbal medicine. Methods : We collected the research paper including killing or inhibiting proliferation of Cancer cells in OASIS and KISS using keyword anticancer drug with herbal medicine, tumor suppressor with herbal medicine, inhibition of Cancer with herbal medicine and combined treatment with herbal medicine. Assorting by cancer cells, we analyzed experimental results cancer cell viability, anticancer drug dosage, tumor weight and survival rate. Also, we checked the effects of herbal medicine on cancer and additive effect reducing side effect of anticancer drug. Results : Total 45 studies were selected. 38 studies reported combined treatment of anticancer drug and herbal medicine was more effective than only anticancer drug. The death of cancer cells was synergistically induced by the cotreatment of anticancer drug and herb extracts. The studies suggest that the cotreatment of anticancer drug and herb extracts could reduce side effect of anticancer drug. In addition, some studies reported cotreatment mechanism like apoptotic death signal processes. In combined treatment of anticancer drug and herb extracts, The expression of Fas/Fas L, Bax, Bcl2, Caspase-3 etc.. was markedly increased in cancer cells. Conclusions : Our results suggest that anticancer drug combined treatment with herbal medicine could be efficient for killing or inhibiting proliferation of cancer cells. However, this paper had some limitation as follows: First, collected studies have been published only for korean journal. Second, results of research and effects of combined treatment are not collected objectively. To solve these problems, more objective and balanced studies should be performed.

• 환경위생공학
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• 제11권1호
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• pp.45-55
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• 1996

An experimental research was conducted in order to study the combined treatment o of municipal landfill leachate and municipal sewage. The landfill leachate was that of Nanjido landfill site, and the municipal sewage was that of Chungnang municipal sewage treatment plant in Seoul. Several sets of bench~scale sequencing batch reactor(SBR) were used as e experimental apparatus. Specially investigated items in this experiment were the removal efficiency of substrate and the influence of treatment time. The experiment lasted for about 2 years. The result are as follows ; 1. The characteristics of leachate were pH 7.5~8.2, BOD 80~336mg/L, COD 908~1,460mg/L, NH3-N 1,409~2,330mg/L, T~P 2.7~7.lmg/L, Cl~3,540~4,085mg/L, a and heavy metals are a very small amount. And the characteristics of sewage were pH 6.9~7.3, BOD 78.4~129.3mg/L, COD 121.2~305.0mg/L, T~N 14.9~36.4mg/L, T-P 2.3~8.9mg/L. 2. The treatability of leachate alone was not treat well. So for the good treatment of leachate, it was necessary to deal with the pretreatment before bi이ogical treatment and a combined treatment of municipal sewage. 3. The various contents of the leachate were 5%, 10%, 30%, and 50%, and the removal efficiency of COD was 86.0%, 82.8%, 60.6%, and 31.7%. The maximum content of the leachate which could be sucessfully treated by SBR in the combined treatment was 10% of that of sewage. And the removal efficiency of COD increased n notably, as its treatment time increased. 4. The various contents of the electrolytic treated leachate were 5%, 10%, 30%, and 50%, and the removal efficiency of COD was 89.9%, 86.1%, 79.2%, and 69.8%. The maximum content of the leachate which could be sucessfully treated by SBR in the combined treatment was 30 % of that of sewage. And the removal efficiency of C COD increased notably, as its treatment time increased.

• 한국전문물리치료학회지
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• 제10권1호
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• pp.129-137
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• 2003

A single subject experimental design (alternating treatment design) was used to compare the effects of only ultrasound and ultrasound combined with stretching of the joint capsule on the ROM increase and pain reduction in patients with frozen shoulder. Two subjects were included in each group. In the only ultrasound treatment sessions, ultrasound was applied at the pain point of the shoulder joint in supine position. In the ultrasound combined with stretching treatment sessions, ultrasound was applied at the pain point of the shoulder joint positioned in external rotation and abduction in sitting position. Only ultrasound treatment and ultrasound combined with stretching treatment were alternately performed on each patient. Pain and disability was measured by shoulder pain and disability index (SPADI), and range of motion (ROM) was measured by scratch test. The results of this study showed that ultrasound combined with stretching treatment were more effective than only ultrasound treatment in ROM increase and pain reduction. However, disability score was not significantly different.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1649-1654
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• 2013

Objective: This work aimed to evaluate the safety and clinical efficacy of transcatheter arterial chemoembolization (TACE) combined with c-arm cone-beam CT guided synchronous radiofrequency ablation (RFA) in treatment of large hepatocellular carcinoma (HCC). Methods: 21 patients with large HCC were studied from January 2010 to March 2012. TACE combined with synchronous C-arm cone-beam CT guided RFA were performed on a total of 25 lesions. Conventional imaging examination (CEUS, enhanced CT or MRI) and AFP detection were regularly conducted to evaluate the technical success rate of combined treatment, complications, treatment response, time without disease recurrence and survival rate. Results: The technical success rate of combined treatment was 100%, without any significant complication. After 1 month, there were 19 cases with complete response and 2 cases with partial response, with an complete response rate of 90.4% (19/21) and a clinical effective rate of 100% (21/21). The complete response rates of single nodular lesions (100%, 17/17) was significantly higher than that of multiple nodular lesions (50%, 2/4) (P<0. 05). During 2 to 28 months of follow-up, in 19 cases with complete response, the average time without disease recurrence was $10.8{\pm}6$ months. The total survival rates of 6, 12 and 18 months in 21 patients were 100%, respectively. Conclusion: TACE combined with synchronous C-arm CT guided RFA is safe and effective for treatment of large HCC. The treatment efficacy for single nodular lesion is better than that for multiple nodular lesions.