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http://dx.doi.org/10.5999/aps.2017.44.3.194

Hydrogel and Platelet-Rich Plasma Combined Treatment to Accelerate Wound Healing in a Nude Mouse Model  

Park, Yu Gil (Department of Plastic and Reconstructive Surgery, Bucheon Hospital, Soonchunhyang University College of Medicine)
Lee, In Ho (Onjin-gun Public Health Center)
Park, Eun Soo (Department of Plastic and Reconstructive Surgery, Bucheon Hospital, Soonchunhyang University College of Medicine)
Kim, Jin Young (Department of Plastic and Reconstructive Surgery, Bucheon Hospital, Soonchunhyang University College of Medicine)
Publication Information
Archives of Plastic Surgery / v.44, no.3, 2017 , pp. 194-201 More about this Journal
Abstract
Background Platelet-rich plasma (PRP) contains high concentrations of growth factors involved in wound healing. Hydrogel is a 3-dimensional, hydrophilic, high-molecular, reticular substance generally used as a dressing formulation to accelerate wound healing, and also used as a bio-applicable scaffold or vehicle. This study aimed to investigate the effects of PRP and hydrogel on wound healing, in combination and separately, in an animal wound model. Methods A total of 64 wounds, with 2 wounds on the back of each nude mouse, were classified into 4 groups: a control group, a hydrogel-only group, a PRP-only group, and a combined-treatment group. All mice were assessed for changes in wound size and photographed on scheduled dates. The number of blood vessels was measured in all specimens. Immunohistochemical staining was used for the analysis of vascular endothelial growth factor (VEGF) expression. Results Differences in the decrease and change in wound size in the combined-treatment group were more significant than those in the single-treatment groups on days 3, 5, 7, and 10. Analysis of the number of blood vessels through histological examination showed a pattern of increase over time that occurred in all groups, but the combined-treatment group exhibited the greatest increase on days 7 and 14. Immunohistochemical staining showed that VEGF expression in the combined-treatment group exhibited its highest value on day 7. Conclusions This experiment demonstrated improved wound healing using a PRP-hydrogel combined treatment compared to either treatment individually, resulting in a decrease in wound size and a shortening of the healing period.
Keywords
Platelet-rich plasma; Hydrogel; Tissue scaffold; Wound healing;
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1 Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001;107:229-37.   DOI
2 Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004;62:489-96.   DOI
3 Bhanot S, Alex JC. Current applications of platelet gels in facial plastic surgery. Facial Plast Surg 2002;18:27-33.   DOI
4 Abegao KG, Bracale BN, Delfim IG, et al. Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits. Acta Cir Bras 2015;30:209-15.   DOI
5 Ito R, Morimoto N, Pham LH, et al. Efficacy of the controlled release of concentrated platelet lysate from a collagen/gelatin scaffold for dermis-like tissue regeneration. Tissue Eng Part A 2013;19:1398-405.   DOI
6 Morimoto N, Yoshimura K, Niimi M, et al. Novel collagen/gelatin scaffold with sustained release of basic fibroblast growth factor: clinical trial for chronic skin ulcers. Tissue Eng Part A 2013;19:1931-40.   DOI
7 Montoya TI, Acevedo JF, Smith B, et al. Myogenic stem cellladen hydrogel scaffold in wound healing of the disrupted external anal sphincter. Int Urogynecol J 2015;26:893-904.   DOI
8 Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg 2006;118:147e-159e.   DOI
9 Hahn HM, Jeon YR, Rha DK, et al. Acceleration of wound healing using adipose-derived stem cell therapy with platelet concentrates: platelet-rich plasma (PRP) vs. Platelet-rich Fibrin (PRF). J Korean Soc Plast Reconstr Surg 2011;38:345-50.
10 Martinez-Zapata MJ, Marti-Carvajal AJ, Sola I, et al. Autologous platelet-rich plasma for treating chronic wounds. Cochrane Database Syst Rev 2012;10:CD006899.
11 Sugimori E, Shintani S, Ishikawa K, et al. Effects of apatite foam combined with platelet-rich plasma on regeneration of bone defects. Dent Mater J 2006;25:591-6.   DOI
12 Serra R, Buffone G, Dominijanni A, et al. Application of platelet-rich gel to enhance healing of transmetatarsal amputations in diabetic dysvascular patients. Int Wound J 2013;10:612-5.   DOI
13 Miyoshi M, Kawazoe T, Igawa HH, et al. Effects of bFGF incorporated into a gelatin sheet on wound healing. J Biomater Sci Polym Ed 2005;16:893-907.   DOI
14 Wang C, Varshney RR, Wang DA. Therapeutic cell delivery and fate control in hydrogels and hydrogel hybrids. Adv Drug Deliv Rev 2010;62:699-710.   DOI
15 Birkenhauer E, Neethirajan S. A double-edged sword: the role of VEGF in wound repair and chemoattraction of opportunist pathogens. Int J Mol Sci 2015;16:7159-72.   DOI
16 Schilling JA, Favata BV, Radakovich M. Studies of fibroplasia in wound healing. Surg Gynecol Obstet 1953;96:143-9.
17 Banks RE, Forbes MA, Kinsey SE, et al. Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology. Br J Cancer 1998;77:956-64.   DOI