• Preventive Nutrition and Food Science
• /
• 제12권3호
• /
• pp.167-172
• /
• 2007

This study was conducted to determine the effect of hygienic processing (HP) on the reduction of microorganisms during manufacturing of saengshik with two vegetables (carrots and cabbage) and two grains (barely and glutinous rice) compared to general processing (GP). For GP, distilled water was used for washing raw materials and equipment. For HP, aqueous ozone (3 ppm) in combination with 1% citric acid and 70% alcohol were used for washing raw materials and the equipment, respectively. In carrots, after cutting, total aerobic bacteria (TAB), yeast and mold (YM) and coliforms were significantly increased to 5.19, 8.04 and 2.08 ($log_{10}$ CFU/g), respectively (p<0.05). Washing effectively reduced the increased microorganisms from cross contamination during cutting, but cross contamination increased with subsequent GP drying and milling procedures to 8.56, 8.27 and 3.71 ($log_{10}$ CFU/g) for TAB, YM and coliforms, respectively (p<0.05). On the other hand, HP washing of carrots with 3 ppm ozone in combination with 1% citric acid showed higher antimicrobial effect than GP washing, significantly decreasing the number of microorganisms (p<0.05). Further cross contamination did not occur through drying and milling due to cleaning the equipments with 70% alcohol prior to processing. After milling, the number of TAB, YM and coliforms were significantly decreased to 3.89, 4.47 and not detectable level ($log_{10}$ CFU/g), respectively (p<0.05). Similar results were observed in cabbage and grains. During storage for two months at different temperatures (22 or $4^{\circ}C$), there were no changes in numbers of spoilage microorganisms in the packaged saengsik after either processing. This suggests the importance of HP for the reduction of microorganisms during saengsik production, and demonstrates the effectiveness of disinfection at each processing stage in minimizing contamination levels to enhance microbial safety of saengshik products.

• Archives of Plastic Surgery
• /
• 제35권5호
• /
• pp.507-513
• /
• 2008

Purpose: Interest in the augmentation of hair growth for functional and aesthetic purpose has increased dramatically in recent years. Many hair growth products have been released, but most of these have not been proven scientifically. This study aims to measure the hair growth effect of azelaic acid and vitamin $B_6$, which have been known as hair growth materials, in animal models. Methods: Six weeks old C57BL/6 mice were used in this study and hair of mice were removed by topical treatment. The mice were divided into five experimental groups according to the testing material such as saline (negative control), propylene glycol(vehicle control), azelaic acid, vitamin B6 and azelaic acid plus vitamin B6 in combination. Hair growth was documented photographically and histologically, and then analysed by the high quality hair analysis program system. The quantity of endocrine factors, IGF-I and TGF-${\beta}1$ in the skin of mice was measured by PCR analysis. Results: The topical treatment of azelaic acid and vitamin B6 in combination for 2 weeks to dorsal skin accelerated hair regrowth more than other groups. The azelaic acid and vitamin $B_6$-combined treatment also promoted hair follicle elongation and thickness compared to the others. Histologic studies showed increased number of basal cells in azelaic acid and vitamin $B_6$-combined treatment. Furthermore, the azelaic acid and vitamin $B_6$-combined group significantly increased the expression of IGF-I but decreased the expression of TGF-${\beta}1$ in the skin of mice compared to other groups. Conclusion: These results suggest that azelaic acid and vitamin $B_6$, when used together, have an additive effect and might be used as hair growth materials.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권9호
• /
• pp.1444-1452
• /
• 2007

An in vitro experiment was conducted to evaluate the differences in microbial activity of five faecal inocula from weaned pigs and one faecal inoculum from unweaned pigs in combination with 6 substrates. The substrates tested were negative control diet, corn, soybean meal, oligofructose (OF), ground chicory roots and a mixture (60% chicory pulp and 40% OF). The inocula used were derived from pigs fed either a corn-soy based diet without antibiotics (NCON), the NCON diet supplemented with oligofructose (OF), a mixture of chicory pulp (40%) and OF (60%) (MIX), ground chicory roots (CHR) or the NCON diet supplemented with antibiotics (PCON). The cumulative gas production measured fermentation kinetics and end products, such as total gas production, ammonia and volatile fatty acids, were also determined. Both the substrate and the inoculum significantly affected the fermentation characteristics. The cumulative gas production curve showed that different substrates caused more differences in traits of fermentation kinetics than the different inocula. Inocula of weaned pigs gave a significantly higher VFA production compared to the inoculum from unweaned animals, whilst the rate of fermentation and the total gas produced did not differ. OF showed the highest fermentation kinetics and the lowest $NH_3$, pH and OM loss compared to other substrates. It was concluded that the microbial activity was significantly affected by substrate and inoculum. Inoculum from weaned pigs had more potential for microbial fermentation of the carbohydrate ingredients and oligofructose than that of unweaned pigs. A combination of high and low polymer inulin may be more beneficial to the gut ecosystem than using high- or low-polymer inulin alone.

• 한국축산식품학회지
• /
• 제34권2호
• /
• pp.178-184
• /
• 2014

The application of ganghwa mugwort (GM), ascorbic acid (AC), and their combinations for reduction of residual nitrite contents was analyzed in pork sausages during storage of 28 d. Six treatments of pork sausages contained the following: Control (no antioxidant added), AC (0.05% AC), GM 0.1 (0.1% GM), GM 0.2 (0.2% GM), AC+GM 0.1 (0.05% AC + 0.1% GM) and AC+GM 0.2 (0.05% AC + 0.2% GM). Results showed that the mixture of 0.05% AC and 0.2% GM was most effective for reducing thiobarbituric acid reactive substances (TBARS) and residual nitrite contents than the control and GM added sausages alone (p<0.05). The color values of all treatments were significantly affected by adding GM (either alone or with AC). Additionally, the total color difference (${\Delta}E$) and hue angle ($H^{\circ}$) values of treatments added with GM were higher than those of the control as the amount of GM increased (p<0.05). However, there were no significant differences in the pH values between the control and all treatments during the storage period (p>0.05). Our results showed possible applications of antioxidant combination, for preventing the lipid oxidation and decreasing the residual nitrite levels of meat products.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권14호
• /
• pp.5529-5533
• /
• 2014

Background: The aim of this study was to investigate the effect of a c-myc antisense oligodeoxynucleotide and 5-fluorouracil on the expression of c-myc, invasion and proliferation of HEPG-2 liver cancer cells. Materials and Methods: HEPG-2 cells were treated with lipiosome-mediated c-myc ADSON and 5-fluorouracil. The proliferation inhibition rate and invasion were measured by MTT and invasion assay, respectively. Cell apoptosis was detected by flow cytometry and expression of c-myc by RT-PCR and immunohistochemistry. Results: The proliferation inhibition rate was significantly higher in the antisense oligodeoxynucleotide added-5-fluorouracil group than single antisense oligodeoxynucleotide or 5-fluorouracil group (p<0.05). G0/G1 cells in the antisense oligodeoxynucleotide group and S cells in the 5-fluorouracil groups were significantly increased than that in the control group, respectively (P<0.01). The amplification strips of PCR products in 5-FU, ASODN and combination groups were significantly weaker than that in the control group (P<0.01). The percentage of c-myc-protein-positive cells were significantly lower in antisense oligodeoxynucleotide, 5-fluorouracil and combination groups than that in the control group (P<0.01). Conclusions: A liposome-mediated c-myc antisense oligodeoxynucleotide and 5-fluorouracil can inhibit the proliferation and invasion of liver cancer cells by reducing the expression of c-myc. A c-myc antisense oligodeoxynucleotide can increase the sensitivity of liver cancer cells to 5-fluorouracil and decrease the dosage of the agent necessary for efficacy, providing an experimental basis for the clinical therapy of liver cancer.

• 한국미생물·생명공학회지
• /
• 제38권4호
• /
• pp.405-413
• /
• 2010

무화과는 뽕나무 과에 속하며 세계에서 가장 오래된 과일 중 하나이고, 국내에서는 주로 소화장애, 염증성궤양 그리고 가려움증 등에 사용되어 왔다. 본 연구에서는 무화과 잎을 메탄올로 추출한 후 임상진료실에서 분리된 메티실린 내성 황색포도상구균(MRSA)에 대한 항균활성을 보았다. 그 결과 분리된 MRSA 1-20에서 최소억제농도 (MIC)는 2.5 mg/mL에서 20 mg/mL 농도로 측정되었으며, 최소살균농도(MBC)는 5 mg/mL에서 20 mg/mL 농도로 관찰되었다. Oxacillin이나 ampicillin과 병용투여 시 4-8배 이상의 MIC/MBC농도가 감소되었으며, FICI 값도 0.0375-0.5이하로 병용효과를 나타내었다. 더불어 메탄올추출물 단독 사용시 보다 항생제와 병용투여 시 빠른 사멸효과를 보였다. 결론적으로 무화과 잎이 메티실린 내성 황색포도상구균에 항균활성을 나타냄을 확인하였으며, 메티실린 내성 황색포도상구균 감염에 천연 치료제로서의 가능성을 확인하였다.

• 융합정보논문지
• /
• 제10권8호
• /
• pp.179-186
• /
• 2020

본 연구에서는 지압과 온열이 조합된 지압온열침대를 사용하여 뇌파와 맥파의 생체신호에 미치는 영향을 알아보고자 하였다. 건강한 성인 40명을 대상으로 조합자극침대를 사용하는 실험군 20명과 무자극의 대조군 20명으로 나누었다. 실험 결과, 뇌파는 실험군에서 델타파와 세타파에서 유의미하게 증가하였고 알파파와 베타파는 유의미하게 감소하였다. 맥파는 실험군에서 부교감신경활성지표가 증가하였으며 심박은 감소하였다. 대조군에서는 아무런 차이가 없었다. 이러한 뇌파의 변화는 조합자극침대가 수면유도의 효과를 주는 것으로 볼 수 있으며 맥파의 변화는 이완의 효과가 나타났다고 할 수 있다. 이러한 연구는 다양한 조합자극기기와 기능성 침대 제품개발에 기초 데이터로 활용될 것으로 기대된다.

• 통합자연과학논문집
• /
• 제6권1호
• /
• pp.34-38
• /
• 2013

Tributyltin hydride ($n-Bu_3SnH$), an endocrine disruptor, was slowly polymerized by the group 4 ${Cp^{\prime}}_2TiCl_2/N$-selectride (Cp' = $C_5H_5$, Cp; $Me-C_5H_4$, Me-Cp; $Me_5C_5$, $Cp^*$) catalyst combination to give two phases of products: one is an insoluble cross-linked solid, polystannane in 3-25% yield as minor product via disproportionation/dehydrocoupling combination process, and the other is an oil, hexabutyldistannane in 65-90% yield as major product via simple dehydrocoupling process. Disproportionation/dehydrocoupling process first produced a low-molecular-weight oligostannane possessing partial backbone Sn-H bonds which then underwent an extensive cross-linking reaction of backbone Sn-H bonds, resulting in the formation of an insoluble polystannane. The disproportionation/dehydrocoupling of a tertiary hydrostannane mediated by early transition metallocene/inorganic hydride is quite unusual and applicable.

• 펄프종이기술
• /
• 제44권4호
• /
• pp.51-61
• /
• 2012

The new alkali pulping process combined with electron beam treatment was applied to utilize hemp bast tissues as a new valuable fibrous resource. Hemp bast tissues have some chemical properties with high lignin contents and holocellulose not to be defiberized by alkali pulping only, compared with the bast tissue of paper mulberry. To make up for the weakness of traditional alkali pulping process, electron beams were directly irradiated into the swelled bast tissue of hemp in NaOH solution and distilled water, and then facilitated the defiberization of hemp bast tissues. The papermaking from hemp bast fibers manufactured by the combination pulping process showed good apparent density, formation structure and air permeability, and had some mechanical properties with lower tensile, tear, burst strength and folding endurance. It is finally concluded that the combination pulping process with electron beam treatment could be suggested a new alternative for non-woody fibers.

• 한국식품과학회지
• /
• 제45권1호
• /
• pp.120-125
• /
• 2013

유리섬유를 이용하여 B. cereus의 biofilm 형성 조건을 확인한 결과 3시간 이후부터 부착하여 48시간에 biofilm이 많이 형성된 것을 확인하였다. 그리고 biofilm 형성 B. cereus의 제어를 위해 에탄올, NaCl, 유기산을 각각 단독으로 처리하였을 때 1% 젖산, 아세트산, 구연산을 30분 처리하였을 때 약 5-6 log CFU/g-glass wool 수준 감소하여 가장 좋은 효과를 나타냈다. 또한, 20% 에탄올과 각각의 1% 유기산, 20% 에탄올과 10% NaCl의 두 가지를 혼합 처리의 경우 단독으로 처리했을 때보다 저감화 효과가 우수했으며, 20% 에탄올과 10% NaCl 및 각각의 1% 유기산을 1-5분 동안 혼합 처리 했을 때 약 7-8 log CFU/g-glass wool 수준이 감소하여 biofilm 형성 B. cereus의 제어에 가장 효과적이었다. 따라서 본 연구 결과를 통해 유기산, 에탄올, NaCl 복합 처리 방법을 사용하여 biofilm 형성 B. cereus의 저감화를 효과적으로 할 수 있을 것으로 판단된다.