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Synergistic Antibacterial Activity of Fig (Ficus carica) Leaves Extract Against Clinical Isolates of Methicillin-resistant Staphylococcus aureus  

Lee, Young-Soo (Department of Dental Hygiene, Sunmoon University)
Cha, Jeong-Dan (Department of Dental Hygiene, College of Natural Sciences, Dongeui University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.4, 2010 , pp. 405-413 More about this Journal
Abstract
Fig (Ficus carica L.) belongs to the mulberry tree (Moraceae) which is one of the oldest fruits in the world. It has been used as a digestion promoter and a cure for ulcerative inflammation and eruption in Korea. The present study investigated the antimicrobial activity of methanol (MeOH) extract of fig leaves against methicillin-resistant Staphylococcus aureus (MRSA) isolated in clinic. The MeOH extract (MICs, 2.5 to 20 mg/mL; MBCs, 5 to 20 mg/mL) was demonstrated as antibacterial activity in isolates MRSA 1-20. The administration of the MeOH extract in combination with oxacillin or ampicillin induced a reduction of ${\geq}$4-8-fold in all tested bacteria, which was considered to be synergistic based on a FICI of ${\leq}$0.375-0.5. Furthermore, time-kill study was found that a combination of MeOH extract with oxacillin or ampicillin produced a more rapid decrease in the concentration of bacteria CFU/mL than MeOH extract alone. The results suggest that fig leaves could be employed as a natural antibacterial agent in MRSA infection care products.
Keywords
Ficus carica (fig); methicillin-resistant Staphylococcus aureus; antibacterial activity; minimum inhibitory concentration; minimum bactericidal concentration; synergistic effect;
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