• 대한전자공학회논문지SP
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• 제41권2호
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• pp.23-34
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• 2004

일반적으로 이미지를 획득하기 위한 도구로 사용되고 있는 비디오 카메라는 동일한 피사체의 XYZ 3 자극치에 대한 RGB 출력 신호가 카메라마다 각각 다른 기기 의존적인 특성을 가진다. 카메라로 촬영한 원 피사체의 XYZ 3 자극치와 카메라 RGB 출력 신호 사이의 변환 관계인 카메라 전달 특성을 알 수 있다면 다양한 디스플레이 시스템에서의 기기 독립적 색 재현이 가능하다. 카메라 전달 특성 조사 방법에 대한 연구는 두 가지 형태로 나뉘어서 진행되어 왔다. 첫 번째 방법은 카메라의 RGB 필터 분광 특성을 파장별로 구하여 CIE 칼러 매칭 함수로 표현하는 것과 알고 있는 색 타겟, 즉 샘플색의 XYZ 3 자극치를 카메라로 촬영하고 출력되는 RGB 신호 간의 변환 행렬을 구하는 방법이다. 실험이 용이하여 일반적으로 많이 이용되는 두 번째 방법은 동일한 카메라에 대해서도 촬영 조건에 따라 매번 60개 이상의 시험색을 촬영하여야 하는 번거로움이 있다. 본 논문에서는 특정 촬영 조건 하에서 구한 카메라 전달 특성 변환 행렬을 이용하여, 촬영 조건이 바뀌었을 때 달라지는 카메라의 전달 특성을 추정하는 방법을 제안한다. 제안한 방법은 먼저 다항식 모델링을 이용하여 특정 화이트 밸런스에서의 카메라 전달 특성 3×3 변환 행렬을 구하고, 이 변환 행렬로부터 카메라의 인광체 좌표를 추정한다. 마지막으로구하고자 하는 기준 백색에 따른 카메라의 채널 이득을 계산하여 특정 기준 백색에 화이트 밸런스가 맞춰진 카메라의 전달 특성을 구하였다. 실험 결과 제안한 방법에 의해 구한 카메라의 전달 특성은 기존의 방법으로 구한 3×3 변환 행렬보다 더 좋은 성능을 가지면 카메라의 화이트 밸런스가 달라질 때마다 모든 시험색을 환영하여 구해야 하는 기존의 변환 행렬과 비슷한 성능을 가지는 3×3 변환 행렬을 손쉽고 간단하게 구할 수 있다.장, 간장, 비장 및 맹장 등 체내 장기로부터 접종균의 회수율은 WL이 일령 증가에 따라 점차 증가하였으나 다른 품종들 보다는 현저히 낮았던 반면에 RIR은 80~90% 이상으로 매우 높았으며 WL$\times$RIR 교배종은 WL보다는 다소 높았으나 RIR보다는 상당한 차이로 낮았다. 항체 양성을에 있어서는 WL$\times$RIR이 WL 및 RIR보다도 높게 나타났다 2. Hyline white$\times$ Hyline brown(HwHb) , Hw$\times$ Isa brown(HwIb), Isa white$\times$Ib(IwIb), Iw$\times$Hb(IwIb) 및 Ib$\times$Iw(IbIw) 등 합성 교배종의 가금티푸스에 대한 질병 저항성 HwHb, Hwlb, Iwlb 및 IwHb 등 4가지 합성품종과 IbIw의 역교배종을 공시하여 6주령 시험계에 감염후 2주간에 걸쳐 폐사율, 증체량, 접종균 회수율 및 응집 항체 양성율 등으로 가금티푸스에 대한 질병 저항성을 비교 시험하였던 바, 공시 품종 모두가 가금티푸스에 대해 현저한 질병 저항성이 있는 것으로 인정되었으며 품종간 비교에서는 HwHb가 공시한 다른 품종들보다 더 강한 저항성을 가지고 있었다.의 이미지에 차이가 있을 것이라는 본 연구가 지지됨을 알 수 있다. 본 연구의 한계점으로 1) 본 연구에서는 자극의 조건을 실험대상자가 소유하고 있는 의복에 한계를 두었다. 다양한 이미지의 의복을 선정하지 못한 점이 앞으로 더 연구하여야 할 점으로 본다. 2) 장소선정에 있어서 강남과 강북 서울중심과 지방 등으로 좀 더 세분화하면 다른 결과가 나을 수 있으리라 예상된다. 따라서 성격이나 가치관, 구매성향에 대해 좀

• 대한전자공학회논문지SP
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• 제44권2호
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• pp.46-54
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• 2007

현재 프린터 산업에서는 다른 출력장치에 비해 색역이 작은 프린터의 색역을 확장하기 위해 CMY 이외의 색료를 추가하여 사용하고 있는 추세이다. 그러나 이러한 색료의 추가는 여러 가지의 색료 조합이 하나의 색 자극치를 나타내는 프린터 역 특성화 과정에서 잉여 문제를 일으킨다. 이에 본 논문에서는 색료간의 상관도를 고려하여 이러한 잉여 문제를 해결하는 프린터 역 특성화 방법을 제안한다. 먼저 동일한 색 자극치를 나타내는 여러 가지 색료 조합을 분석하기 위해 Cellular Yule-Nielsen Spectral Neugebauer 프린터 모델을 이용하여 모든 색료의 조합에 대한 색 자극치를 추정한다. 이 후 잉여 문제를 일으키는 색료의 조합들에 대해 색 공간에서의 주위 다른 색 조합들과의 상관도를 고려하여 가장 유사한 색료 조합을 선택한다. 이를 통해 임의의 입력 색 자극 값에 대한 출력 색료 값을 얻는 3차원 사면체 보간법을 사용하는 과정에서 오차를 줄일 수 있다. 이렇게 선택된 색료의 조합들과 색 자극치는 참조표로 저장된다. 실험에서는 CMYKGO 프린터를 사용하였으며 어두운 영역에서 색역이 확장 되었고, 좀 더 자연스러운 톤을 표현하였다.

• Food Science and Biotechnology
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• 제16권3호
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• pp.477-481
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• 2007

Fourier transform near-infrared (FT-NIR) spectroscopy and partial least squares (PLS) regression were used to predict the amylose content of polished rice. Spectral reflectance data in a wavelength range of 1,000 to 2,500 nm were obtained with a commercial spectrophotometer for 60 different varieties of Korean rice. For a comparison of this spectroscopic method to a standard chemical analysis, the amylose contents of the tested rice samples were determined by the iodine-blue colorimetric method. The highest correlation for the rice amylose ($R^2=0.94$, standard error of prediction=0.20% amylose content) was obtained when using the FT-NIR spectrum data pre-treated with normalization, the first derivative, smoothing, and scattering correction.

• 대한화학회지
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• 제12권4호
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• pp.139-141
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• 1968

液狀이온交換體 Amberite LAI을 chloroform에 10%로 묽혀서 鹽酸算性에서 Bi(III), As(III), Sb(III), Zn(II) 및 Fe(III)의 推出率을 試驗한 結果, Zn(II)는 2M에서 98%. As(III)는 7~14M에서 Sb(III)는 2~4M에서 , Fe(III)는 6~10M에서 蒼鉛과 定量的으로 分離되었다. 그리고 chloroform을 溶媒로 썼을 때는 xylene이나 hexane을 溶媒로 썼을때보다 더 急傾斜를 이루는 推出率曲線을 나타냈다. 各各의 不純物을 分離後 Zn(II)는 dithizone. As(III)는 Gutzeit method, Sb(III)는 brilliant green, Fe(III)는 thiocyanate에 依한 比色方法으로 定量하였다.

• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.68-76
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• 2007

Objectives : This experimental study was performed to investigate the effects of Sulfur extract on anti-inflammation and anti-Propionibacterium acnes. Methods : The cytotoxicity of Sulfur extract about viability of Raw 264.7 cell were tested using a colorimetric tetrazolium assay(MTT assay). To investigate the anti-inflammation effects of Sulfur extract on LPS-induced macrophage Raw 264.7 cell, we used ELISA kit and Western blots. We evaluated anti-oxidation effects of Sulfur extract on HaCaT cell by Enzyme recycling method. And we investigated the inhibitory effects of Sulfur extract on Propionibactrium acnes using paper disk diffusion method. Results: 1. Sulfur extract has a little cytotoxicity in Raw 264.7 cell. 2. Concentration of $100\;{\mu}g/m{\ell}$ Sulfur extract inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 3. Sulfur extract showed a oxidation inhibition effect by decreasing the DPPH radicals. 4. Sulfur extract has not the significant inhibition effect of Propionibactrium acnes. Conclusions: These results indicate that Sulfur extract has anti-inflammation and anti-oxidation effects. If further study is performed, the use of Sulfur extract will be valuable and benificial in the therapy of Propionibactrium acnes.

• Journal of Microbiology and Biotechnology
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• 제34권2호
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• pp.340-348
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• 2024

Salmonella, a major contributor to foodborne infections, typically causes self-limiting gastroenteritis. However, it is frequently invasive and disseminates across the intestinal epithelium, leading to deadly bacteremia. Although the genus is subdivided into >2,600 serotypes based on their antigenic determinants, only few serotypes are responsible for most human infections. In this study, a rapid dot-blot immunoassay was developed to diagnose multiple Salmonella enterica serotypes with high incidence rates in humans. The feasibility of 10 commercial antibodies (four polyclonal and six monoclonal antibodies) was tested using the 18 serotypes associated with 67.5% Salmonella infection cases in the United States of America (U.S.A) in 2016. Ab 3 (polyclonal; eight of 18 serotypes), Ab 8 (monoclonal; 13 of 18 serotypes), and Ab 9 (monoclonal; 10 of 18 serotypes) antibodies exhibited high detection rates in western blotting and combinations of two antibodies (Ab 3+8, Ab 3+9, and Ab 8+9) were applied to dot-blot assays. The combination of Ab 3+8 identified 15 of the tested 18 serotypes in 3 h, i.e., S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,[5],12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona, and S. Anatum, which were responsible for 53.7% Salmonella infections in the U.S. in 2016. This cost-effective and rapid method can be utilized as an on-site colorimetric method for Salmonella detection.

• 생약학회지
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• 제1권3호
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• pp.93-99
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• 1970

There have been reported by several workers for the isolation and determination of the amine derivatives as Metbylephedrine Hydrochloride and Ephedrine Hydrochloride adopting neutralization method, steam distillation method, non-aqous titration method, ion-exchange resin method, titration method after acetylation, colorimetric method, gravimetric method, iodine titration method and gas chromatography. Those methods mentioned in above, can be practically applied for the sample which is not mixed one mith the other amine compounds. Presently, it has not shown on the isolative determination of the mixed sample of amine derivatives. In this paper, it is discussed on the isolative determination of Methylephedrine Hydrochloride as the tertiary amine compound and Ephedrine Hydrochloride as the secondary amine compound. According to the results of the experiment, it could be summarized as follows: 1. There is no time-variation on the color reaction of Methylephedrine Hydrochloride and Ephedrine Hydrochloride with the color reagent, bromcresolgreen. And Methylephedrine Hydrochloride and Ephedrine Hydrochloride, respectively, can be determined spectrophotometrically by means oft his color reaction. 2. For the isolation of Methylephedrine Hydrochloride and Ephedrine Hydrochloride from the mixed sample, Methylephedrine Hydrochloride can be eluted by chloroform, while Ephedrine Hydrochloride by the mixed solvent of chloroform and ethylalcohol (2:1), from the celite column adsorbed at pH6.4 followed by extraction with ether undersodium hydroxide alkali re action. 3. When the sample is mixed with quinine hydrochloride, dihydrocodeine bitartate, and noscapine, these mixed compounds can be eliminated by means of stram distillation. 4. When the sample is mixed with chlorpheniramine maleate, dextromethorphan hydrobromide and diphenhydramine hydrochloride, the mixed compounds can be eliminated by means of steam distillation and celite adsorption column chromatography, In conclusion, the isolative determination method for Methylephedrine Hydrochloride and Ephedrine Hydrochloride studied in this paper, indicates with the excellent reproducibility and accuracy.

• ALGAE
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• 제31권4호
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• pp.391-401
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• 2016

To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

• 한국연초학회지
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• 제31권1호
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• pp.39-44
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• 2009

Hydrogen cyanide (HCN) is present in both the particulate and vapor phase of cigarette mainstream smoke. It is one of the 44 harmful substances on Hoffmann's list and is known to be a major ciliatoxic agent in cigarette mainstream smoke. Typically the determination of HCN in cigarette mainstream smoke has been done through colorimetric and electrochemical techniques, such as UV-spectrophotometry (UV), continuous flow analyzer (CFA), ion chromatography (IC) and capillary GC-ECD. In particular, CFA commonly has been using analysis hydrogen cyanide in cigarette smoke and the basic principle is pyridine-pyrazolone reaction. In this study, the more optimized analytical method is suggested isonicotinic acid-pyrazolone reaction method than previous pyridine-pyrazolone reaction method, a commonly used method for the determination of cyanide in water and air, by CFA. Sample collection was optimized by trapping particulate and vapor phase of smoke separately. The optimum NaOH concentration of the trapping solution was shown to be 0.2 M. HCN was stable up to 6 hours in this concentration but only 3 hours in 0.1 M solution. The sensitivity of this method was fairly good and it might be used in analysis of HCN in cigarette mainstream smoke.

• Preventive Nutrition and Food Science
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• 제11권1호
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• pp.67-72
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• 2006

To determine the amount of phytate in rice grains from various cultivars, two methods were employed and compared in respect of the accuracy and conveniency. Phytate in rice samples was extracted with HCl, and then the extracts were subjected to an anion-exchange column. Finally, the phytate in eluate was quantitated using two methods: one method is based on the complex formation between ferric ion and sulfosalicylic acid in the presence of phytate, and the other is the prior acid digestion of phytate sample, followed by the colorimetric determination of liberated phosphorus. Although two methods showed similar values of phytate in rice samples, the former method is simpler and more precise than the latter. Moreover, the former is more reliable for the samples with lower phytate levels. Especially, the dilution condition of rice sample before anion exchange column separation was important for the recovery of phytate in rice samples. Based on the former method, the amount of phytate in rice of various cultivars was estimated to range from 7.3 mg/g to 12.4 mg/g rice. This method would be useful for the determination of phytate in crop samples with a lower level of phytate, one of anti-nutrients in some agricultural plants.