• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.5
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• pp.602-609
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• 2016

In a smart society, politicians analyze the big data of voters to build a favorable political positions. In other words, a variety of digital footprints uploaded in SNS or Internet are used to set the election strategies and political directions. In comparison, it is difficult for voters to extract intention information about how politicians are performing a political acts. Therefore, it is important that voters need to analyze what intention of politicians are like for two way interaction between voters and politicians. For this, in this paper, we want to do the identification by analyzing IT technologies to narrative styles of politicians who pursue relative advantages or gains compared to other competitors. The experiments will be carried out to identify about what relative advantages compared to other competitors by narrative styles of next presidential candidates who are expected to run into the next presidential election by analyzing the usual audio interviews.

• The Journal of the Korean Society for Microbiology
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• v.13 no.1
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• pp.25-30
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• 1978

Among clincal isolates, most strains of Serratia marcescens were belonged to nonpigmented form, and several attempts were undertaken for the rapid and simple identification of these strains. Prodigiosin production of non-pigmented strains was uniformly negative in many kinds of solid media as well as in nutrient agar added with various amino acids and thiamine. On blood agar, colonies of S. marcescens turned gradually to grey or dark color by the lapse of incubation period and this characteristic seems to be able to utilize as an indicator for a primary isolation, and also generally paralleled with the results of dehydration of Tween 80 and lipase activity in soy bean oil medium although these reactions were by no means specific to S. marcescens. In order to rule out these non-specific reactions, other tests such as oxidase and sucrose fermentation are required for the final confirmation of this species.

• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.897-902
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• 2004

To improve the methods used for the identification of Hanwoo, we performed a PCR using 3 -tailed primer associated with single nucleotide polymorphism(SNP) in Melanocortin 1 receptor(MCIR) gene. MCIR plays an important role in melanin synthesis and the SNP within MCIR was used as a target for PCRRFLP studies previously. A forward 3 -tailed primer, which matches with the template DNA of Hanwoo but not with others(blackhaired; Holstein and Black angus) at the site of 594th base sequence, and one reverse primer were designed for this study. When use this primer set, a size of 343bp was amplified by PCR only in Hanwoo, not in Holstein and Black angus. This result suggests that the PCR using our 3 -tailed primer would be very accurate, easy, reproducible and economic method to discriminate between Hanwoo meat and other black-haired ones.

• Korean journal of food and cookery science
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• v.24 no.1
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• pp.11-15
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• 2008

In searching for yeast to be utilized as biocontrol agents, a single yeast strain was isolated from Camellia sinensis based on its morphological, cultural, physiological, and biochemical properties, as well as by molecular techniques. This single strain was pink to red in color and designated as strain JY-1. The effects of temperature, pH, NaCl concentration, and ethanol concentration on the growth of the JY-l strain were examined for the JY-1. Growth occurred at temperatures ranging from 20 to $35^{\circ}C$, and between pH 3.0 and 12.0, with optimal growth at $25-30^{\circ}C$ and pH 5.0. The yeast also grew in the presence of 0-2% (w/v) NaCl and 0-4% (v/v) EtOH. The isolate was further classified based on biochemical characteristics using the VITEK system. The biochemical data obtained using this system were similar to those of Rhodotorula glutinis/Rhodotorula mucilaginosa (exhibiting a 93% matching level). Molecular phylogenetic analysis based on l8S rDNA sequences indicated that the yeast represented a basidiomycetous species, and its highest degree of sequence similarity was with Rhodosporidium azoricum, strain JCM11251 (99%).

• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.289-296
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• 2013

Cryptomonads are unicellular, biflagellate algae. Generally, cryptomonad cells cannot be preserved well because of their fragile nature, and an improved methodology should be developed to identify cryptomonads from natural habitats. In this study, we tried using several cytological fixatives, including glutaraldehyde, formaldehyde, and their combinations to preserve field samples collected from various waters, and the currently used fixative, Lugol's solution was tested for comparison. Results showed that among the fixatives tested, glutaraldehyde preserved the samples best, and the optimal concentration of glutaraldehyde was 2%. The cell morphology was well preserved by glutaraldehyde. Cells kept their original color, volume, and shape, and important taxonomic features such as furrow/gullet complex, ejectosomes, as well as flagella could be observed clearly, whereas these organelles frequently disappeared in Lugol's solution preserved samples. The osmotic adjustments and buffers tested could not preserve cell density significantly higher. Statistical calculation showed the cell density in the samples preserved by 2% glutaraldehyde remained stable after 43 days of the fixation procedure. In addition, DNA was extracted from glutaraldehyde preserved samples by grinding with liquid nitrogen and the 18S rDNA sequence was amplified by PCR. The sequence was virtually identical to the reference sequence, and phylogenetic analyses showed very close relationship between it and sequences from the same organism. To sum up, the present study demonstrated that 2% unbuffered glutaraldehyde, without osmotic adjustments, can preserve cryptomonads cells for identification, in terms of both light microscopy and phylogenetic analyses based on DNA sequences.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.1
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• pp.75-81
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• 2012

Road identification is the important task for extracting a road region from the high-resolution satellite images, when the road candidates is extracted by the pre-processing tasks using a binarization, noise removal, and color processing. Therefore, we propose a noble approach for identifying a road using the orientation-selective spatial filters, which is motivated by a computational model of neuron cells found in the primary visual cortex. In our approach, after the neuron cell typed spatial filters is applied to the isolated connected-labeling road candidate regions, proposed method identifies the region of perceiving the strong orientation feature with the real road region. To evaluate the effectiveness of the proposed method, the accuracy&error ratio in the confusion matrix was measured from road candidates including road and non-road class. As a result, the proposed method shows the more than 92% accuracy.

• Journal of the Ergonomics Society of Korea
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• v.26 no.2
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• pp.1-13
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• 2007

Ergonomic methods have been effectively applied to design and evaluation of functional clothing. The goals of the present study are to: (1) develop an ergonomic evaluation system for the systematic analysis of functional clothing and (2) examine the usefulness of the proposed evaluation system by applying to flame-proof clothing. Based on the survey of literature and the brainstorming of experts in clothing design and ergonomics, factors considered for clothing evaluation were selected, classified, and complemented, resulting in an ergonomic clothing evaluation system consisting of four factor categories (clothing construction, user, work and environment, and user response). Using the proposed system, a field survey and a laboratory experiment were conducted for flame-proof clothing to identify its design problems. The field survey to workers found a comprehensive set of problems on the flame-proof clothing design in terms of pattern, textile, and color. The laboratory experiment identified additional design problems using a questionnaire that was developed based on an analysis on the relationship between clothing design components and ergonomic evaluation measures. The present study showed the ergonomic evaluation system and the relationship analysis of clothing design components and ergonomic evaluation measures are of use to identify design problems of functional clothing in a comprehensive and analytic manner.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.265-266
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• 2015

A search for luminous white dwarfs (WDs) in several nearby Galactic globular clusters (GCs) was carried out using the deep and homogeneous photometric catalog of Galactic GCs taken with the ACS/WFC aboard the Hubble Space Telescope (HST) by Sarajedini et al. and Anderson et al- It resulted in the identification of luminous WD candidates in the GCs M13 (NGC 6205) and M22 (NGC 6656). The purpose of the present study is to identify luminous WDs in the deep and homogeneous V versus V - I color-magnitude diagrams (CMDs) of several nearby Galactic GCs taken with the ACS/WFC aboard the HST. Using photometric data for the GCs M13 and M22 that are now in the public domain, the V versus V - I CMDs of the GCs M13 and M22 were constructed. Many spurious detections in the CMDs were removed using the photometric quality parameters qfit(V) and qfit(I), and a radial restriction was applied to the CMDs to remove the central stars with higher photometric errors due to central crowding. From each resultant V versus V - I CMD of the GCs M13 and M22, a dozen or so luminous WD candidates were identified. They were confirmed as stellar objects in the accompanying ACS/WFC images and their positions in the CMDs were in the bright part of the DA WD cooling curve. Therefore, the luminous WD candidates in the GCs M13 and M22 seem to be true luminous WDs, and spectroscopic observations are needed to confirm their true identity.

• Analytical Science and Technology
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• v.13 no.1
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• pp.101-107
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• 2000

The automotive paints could be generally differentiated by color, layer sequence and chemistry of the paint layers comprising each of the topcoat and the primer system. The successful identification of hit-andrun a and traffic accidental vehicles from evidential paint fiagments is greatly facilitated with a comprehensive laboratory collection of reference paint samples and the technique for direct analysis without sample preparation. The Pyrolysis-Gas Chromatography(PGC) is a precise and reliable method for performing both quantitative and qualitative analysis of polymeric materials and forensic samples. Our Forensic Laboratory is conducting the examination and identification of 73 reference paint samples; 4 colors of each domestic automotive make that is popular in Korea, by Curie Point Pyrolyzer(JHP-3) and GC with capillary column(ultra alloy-5). This method can be used not only to compare paint traces with their suspected sources, but also to identify the type, make and model of the automotive car.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 1996.06a
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• pp.33-33
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• 1996

Interests in the field of rapid methods and automation in microbiology have been growing steadily on an international scale in recent years. International meetings concerned this problem have been held in elsewhere in the world countries since the past twenty years. But, unfortunately in the field of microbial examination in food hygiene, this problem have not yet been developed so much as in the field of clinical microbiology. Today, I would like to introduce you here present aspects of rapid and automation technologies, those which are manly carrying in milk and meats industries. My illustration will be given recent improved technologies using automatic apparatus and instruments along with process of microbial count procedure. Recent direct microbiological counting system (ChemeScan \ulcorner) as real time ultrasensitive analysis created by Cheminex Ltd., France is now most evolutional instrument to provide direct microbial counts, down to one cell, within 30 minutes. The results from these evaluations how a good correlation between the ChemScan system and the standard plate count method. This system will be successful application for not only in the field of pharmacology but also food microbiology. In addition, current identification of microbes by sophisticated instruments suitable for food microbiology, one of which Biology is manual system (BIOLOG\ulcorner), provides reference-level capability at a modes price. For the manual system, the color reactions in the microplate are read by eye and manually keyed into personal computer. Species identification appears on the computer screen within seconds, along with biotype patterns, a list of closely related species, and other useful statistics. In present this is useful application for microbial ecology and epidemiological survey. RiboPrinter system newly produced by DuPont is now focusing among microbiologists in the world, and is one of the biggest microbial characterization system using a DNA-based approach. The technology analyzer is bacterial culture for its genetic fingerprint or riboprint pattern. Finally Bio-cellTracer system for automatic measurement of fungal growth and Fukitori-Maseter, a Surface Hygiene Monitoring Kit by using swabe procedure in food processing environment are briefly illustrated in this presentation.