• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2001년도 합동 춘계 학술발표회
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• pp.57-57
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• 2001

• The Plant Pathology Journal
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• 제35권2호
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• pp.156-163
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• 2019

Our study investigated the available chlorine content, contact time and difference among strains of each pathogen for sodium hypochlorite (NaOCl) to control chemically against soil-borne fungal pathogens, such as Phytophthora rot by Phytophthora cactorum, violet root rot by Helicobasidium mompa, and white root rot by Rosellinia necatrix, causing die-back symptom on apple trees. As a result, the colony growth of Phytophthora cactorum was inhibited completely by soaking over 5 s in 31.25 ml/l available chlorine content of NaOCl. Those of H. mompa and R. necatrix were inhibited entirely by soaking over 160 s in 62.5 and 125 ml/l available chlorine content in NaOCl, respectively. Also, inhibition effect on available chlorine in NaOCl among strains of each soil-borne pathogen showed no significant difference and was similar to or better than that of fungicides.

• BMB Reports
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• 제44권10호
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• pp.686-691
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• 2011

Granulocyte colony-stimulating factor (G-CSF) is a cytokine secreted by stromal cells and plays a role in the differentiation of bone marrow stem cells and proliferation of neutrophils. Therefore, G-CSF is widely used to reduce the risk of serious infection in immunocompromised patients; however, its use in such patients is limited because of its non-persistent biological activity. We created an N-linked glycosylated form of this cytokine, hG-CSF (Phe140Asn), to assess its biological activity in the promyelocyte cell line HL60. Enhanced biological effects were identified by analyzing the JAK2/STAT3/survivin pathway in HL60 cells. In addition, mutant hG-CSF (Phe140Asn) was observed to have enhanced chemoattractant effects and improved differentiation efficiency in HL60 cells. These results suggest that the addition of N-linked glycosylation was successful in improving the biological activity of hG-CSF. Furthermore, the mutated product appears to be a feasible therapy for patients with neutropenia.

• 한국연초학회지
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• 제11권2호
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• pp.241-246
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• 1989

Mold development on leaf tobacco(Flue-cured, var. NC 82) during the storage was examined according to the initial moisture content(MC) of the tobacco. The initial moisture content of the leaf tobacco was controlled as 12,14,16% after redrying. Those were packed in cardboard boxes and stored in a warehouse. Samples were taken monthly from June of 1988 through August of 1989 and were evaluated for mold development (colony count) and MC. The moisture content change in the leaf tobacco of 12, 14 and 16% initial MC was in the range of 11.1-13.3, 12.8-15.3 and 14.9-16.7% and the mean number of colonies from them were 1.4$\times$102, 1.0$\times$102, and 4.0$\times$102 colonies per gram of tobacco, respectively, however mold-damaged leaf tobacco other than the treatments was occasionally observed to have the colony number as high as 2.3$\times$105 colonies per gram of tobacco. In the meantime, the leaf tobacco was stored at 6 relative humidity levels at $25^{\circ}C$ in laboratory test. Leaf tobacco of 15.0-16.4% MC was maintained without the mold-damage after 80 days of storage whereas those of 19.9, 21.3, 25.5 and 27.1% MC became moldy after 50, 15, 11 and 6 days of storage, respectively.

• Mycobiology
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• 제39권2호
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• pp.85-91
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• 2011

We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of $25^{\circ}C$ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, $K_3PO_4$ showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and $K_3PO_4$ (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.

• International Journal of Computer Science & Network Security
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• 제21권8호
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• pp.342-352
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• 2021

Multicast Routings is a big challenge due to limitations such as node power and bandwidth Mobile Ad-hoc Network (MANET). The path to be chosen from the source to the destination node requires protocols. Multicast protocols support group-oriented operations in a bandwidth-efficient way. While several protocols for multi-cast MANETs have been evolved, security remains a challenging problem. Consequently, MANET is required for high quality of service measures (QoS) such infrastructure and application to be identified. The goal of a MANETs QoS-aware protocol is to discover more optimal pathways between the network source/destination nodes and hence the QoS demands. It works by employing the optimization method to pick the route path with the emphasis on several QoS metrics. In this paper safe routing is guaranteed using the Secured Multicast Routing offered in MANET by utilizing the Ant Colony Optimization (ACO) technique to integrate the QOS-conscious route setup into the route selection. This implies that only the data transmission may select the way to meet the QoS limitations from source to destination. Furthermore, the track reliability is considered when selecting the best path between the source and destination nodes. For the optimization of the best path and its performance, the optimized algorithm called the micro artificial bee colony approach is chosen about the probabilistic ant routing technique.

• 정보처리학회논문지A
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• 제16A권2호
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• pp.135-142
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• 2009

유비쿼터스 컴퓨팅 기술의 발전에 따라, 사용자 적응적인 서비스를 사용자에게 제공하기 위해 다양한 콘텍스트를 이용하는 HCI 어플리케이션이 점점 증가하고 있다. 또한, 다양한 센싱 정보의 수집과 통합 콘텍스트 생성에 관한 연구의 중요성이 높아지고 있다. 그러나 콘텍스트 생성을 위한 센싱 정보의 수집 및 통합 기술에 관한 연구가 콘텍스트의 활용도에 비해 부족하다. 특히, 기존의 연구들은 통합 콘텍스트 생성에 있어 콘텍스트 중복 및 시스템 부하와 같은 몇 가지 해결해야 할 문제점들을 가지고 있다. 본 논문에서는, 개미 군집 시스템 기반의 통합 콘텍스트 생성 기법을 제안한다. 제안된 기법은 XML 형태의 통합 콘텍스트를 생성한다. 또한, 개미 군집 시스템을 이용하여 중복되는 샘플링 정보를 검출하여 불필요한 콘텍스트의 생성을 피한다. 그 결과로 통합 콘텍스트를 생성할 때 발생되는 자원 및 저장 공간의 낭비를 감소시킬 수 있다. 또한, 적응적인 서비스를 수행하기 위해 필요한 추론 과정의 부하를 감소시킬 수 있다.

• 식물병연구
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• 제29권1호
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• pp.79-81
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• 2023

2021년 5월 화상병 확산 방지를 위해 화상병 예찰 중에 충북 음성의 화상병이발병한 배 과수원 바로 인근의 밭에서 자라는 팥배나무의 어린 가지가 수침상을 보이면서 마르는 증상을 발견하였다. 채집한 잎과 어린 가지로부터 균 분리를 진행한 결과 화상병원균과 유사한 유백색의 mucoid한 colony를 보이는 2개 균주를 순수 분리하였다. 순수 분리된 병원균을 화상병원균 특이 프라이머 PCR 검정 및 16S rRNA 염기서열을 이용한 계통도 분석을 통해 분리된 균주가 Erwinia amylovora임을 확인하였다. 배 유과와 사과 대목에 병원성 검정을 수행한 결과 접종 6일 후에 접종한 자리에 괴사 증상과 우즈를 확인할 수 있었고, 이들 증상에서 동일한 균을 재분리하였다. 본 연구는 국내에서 최초로 E. amylovora에 의해 발생한 팥배나무에서의 화상병을 보고하고자 한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제26권2호
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• pp.124-130
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• 2013

Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of $-2.5^{\circ}C$, $0^{\circ}C$, $2.5^{\circ}C$ and $5^{\circ}C$ with constant humidity >80%, the queens stored at $2.5^{\circ}C$ exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at $0^{\circ}C$, $5^{\circ}C$, $7.5^{\circ}C$ and $12.5^{\circ}C$. At $2.5^{\circ}C$ the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at $5^{\circ}C$. Thus, $2.5^{\circ}C$ and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.

• 한국환경농학회지
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• 제17권4호
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• pp.306-311
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• 1998

Effects of soluble silicon and surfactants on the development of powdery mildew of cucumber were tested for environmentally safe powdery mildew control. Tested soluble silicon was potassium silicate$(K_2SiO_3)$ and tested commercial surfactants were Jeonchakje, Silhouette, Kaba, and Tween 20. Tested concentrations were 2, 4, 8, 16mM for the soluble silicon, 0.5 and 1.0% for Tween 20, 0.05 and 0.1% for both Jeonchakje and Kaba, and 0.03 and 0.07% for Silhouette. Water dilutions of tested materials were sprayed on cucumber (Cucumis sativus) leaves once a week for 4 weeks. From 3 days after the second spray, the diseased area and the numbers of fungal colony were measured from the treated leaves for 6 times at 3-4 days interval. Powdery mildew was less severe on treated cucumber compared to distilled water-treated cucumber (check). In all treatments, diseased area index was proportional to the number of the fungal colony. At the end of investigation, there was severe powdery mildew on check cucumbers. Average 30% of a leaf was colonized by powdery mildew fungus and the average number of the fungal colony per leaf reached to more than 70. On the other hand, none of the treated cucumber suffered severe powdery mildew. The treatments including 8 and 16mM of soluble silicon, doubled concentration of Kaba, and 0.5 and 1.0% of Tween 20 showed more than 80% of control effect compared to water-treated cucumber. In addition, no phytotoxicity was found. Potassium silicate and Tween 20 showed the possibility to replace chemical pesticides for the control of powdery mildew.