Browse > Article
http://dx.doi.org/10.4489/MYCO.2011.39.2.085

Growth and Cultural Characteristics of Ophiocordyceps longissima Collected in Korea  

Sung, Gi-Ho (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Shrestha, Bhushan (Green Energy Mission/Nepal)
Han, Sang-Kuk (Division of Forest Biodiversity, Korea National Arboretum)
Sung, Jae-Mo (Cordyceps Institute of Mushtech)
Publication Information
Mycobiology / v.39, no.2, 2011 , pp. 85-91 More about this Journal
Abstract
We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of $25^{\circ}C$ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, $K_3PO_4$ showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and $K_3PO_4$ (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.
Keywords
Carbon source; Growth characteristics; Mineral salt; Nitrogen source; Ophiocordyceps longissima;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Yahagi N, Yahagi R, Takano F, Fushiya S, Tanaka T, Murakami K, Ohta T. Growth of ascocarps from cultured Cordyceps militaris (L.:Fr.) Fr. and Cordyceps formicarum Kobayasi in an agar medium. Nippon Kingakkai Kaiho 2004;45:15-9.
2 Li CR, Nam SH, Geng DG, Fan MZ, Li ZZ. Artificial culture of seventeen Cordyceps spp. Mycosystema 2006;25:639-45.
3 Lee JO, Shrestha B, Kim TW, Sung GH, Sung JM. Stable formation of fruiting body in Cordyceps bassiana. Mycobiology 2007;35:230-4.   과학기술학회마을   DOI   ScienceOn
4 Lee JO, Shrestha B, Sung GH, Han SK, Kim TW, Sung JM. Cultural characteristics and fruiting body production in Cordyceps bassiana. Mycobiology 2010;38:118-21.   과학기술학회마을   DOI   ScienceOn
5 Kim SY, Shrestha B, Sung GH, Han SK, Sung JM. Optimum conditions for artificial fruiting body formation of Cordyceps cardinalis. Mycobiology 2010;38:133-6.   과학기술학회마을   DOI   ScienceOn
6 Sung GH, Shrestha B, Park KB, Sung JM. Cultural characteristics of Shimizuomyces paradoxus collected from Korea. Mycobiology 2010;38:189-94.   과학기술학회마을   DOI   ScienceOn
7 Shrestha B, Lee WH, Han SK, Sung JM. Observations on some of the mycelial growth and pigmentation characteristics of Cordyceps militaris isolates. Mycobiology 2006;34:83-91.   과학기술학회마을   DOI   ScienceOn
8 Sung GH, Shrestha B, Han SK, Kim SY, Sung JM. Growth and cultural characteristics of Cordyceps cardinalis collected from Korea. Mycobiology 2010;38:274-81.   과학기술학회마을   DOI   ScienceOn
9 Sung GH, Shrestha B, Han SK, Sung JM. Cultural characteristics of Ophiocordyceps heteropoda collected from Korea. Mycobiology 2011;39:1-6.   과학기술학회마을   DOI   ScienceOn
10 Mizuno T. Medicinal effects and utilization of Cordyceps (Fr.) Link (Ascomycetes) and Isaria Fr. (Mitosporic fungi) Chinese caterpillar fungi, "Tochukaso" (Review). Int J Med Mushrooms 1999;1:251-61.   DOI
11 Kinjo N, Zang M. Morphological and phylogenetic studies on Cordyceps sinensis distributed in southwestern China. Mycoscience 2001;42:567-74.   DOI   ScienceOn
12 Shrestha B, Zhang W, Zhang Y, Liu X. What is the Chinese caterpillar fungus Ophiocordyceps sinensis (Ophiocordycipitaceae)? Mycology 2010;1:228-36.   DOI
13 Yahagi N. Cultivation of Isaria japonica and inoculation to silkworm. Touchu-Kasou 1985;5:27-30.
14 Harada Y, Akiyama N, Yamamoto K, Shirota Y. Production of Cordyceps militaris fruit body on artificially inoculated pupae of Mamestra brassicae in the laboratory. Trans Mycol Soc Jpn 1995;36:67-72.
15 Yamanaka K, Inatomi S. Cultivation of Isaria japonica fruit bodies on mixed plant/insect media. Food Rev Int 1997;13:455-60.   DOI   ScienceOn
16 Yamanaka K, Inatomi S, Hanaoka M. Cultivation characteristics of Isaria japonica. Mycoscience 1998;39:43-8.   DOI   ScienceOn
17 Kana-uchi A, Fukatsu T. Light-induced fruit body formation of an entomogenous fungus Paecilomyces tenuipes. Mycoscience 1999;40:349-51.   DOI   ScienceOn
18 Chen R, Ichida M. Infection of the silkworm, Bombyx mori, with Cordyceps militaris. J Insect Biotechnol Sericology 2002;71:61-3.
19 Kobayasi Y. On the genus Cordyceps and its allies on Cicadidae from Japan. Bull Biogeogr Soc Jpn 1939;9:145-76.
20 Sato H, Shimazu M. Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Appl Entomol Zool 2002;37:85-92.   DOI   ScienceOn
21 Kobayasi Y, Shimizu D. Monographic studies of Cordyceps 2: Group parasitic on Cicadidae. Bull Natl Sci Mus Tokyo 1963;6:286-314.
22 Sung JM. The insects-born fungus of Korea in color. Seoul: Kyohak Publishing Co., Ltd.; 1996.
23 Lee JB, Oh DC. Higher fungi of Cheju-do (1): unrecorded mushrooms. Kor J Mycol 1998;26:538-50.   과학기술학회마을
24 Li CR, Huang B, Fan MZ, Li ZZ. Cordyceps longissima and its Hirsutella anamorph. J Anhui Agric Univ 1999;26:374-7.
25 Li CR, Fan MZ, Huang B, Li ZZ. Hirsutella longissima sp. nov., the anamorph of Cordyceps longissima. Mycosystema 2001;20:29-34.
26 Sung GH, Shrestha B, Sung JM. Characteristics of Metacordyceps yongmunensis, a new species from Korea. Mycobiology 2010;38:171-5.   과학기술학회마을   DOI   ScienceOn
27 Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 2007;57:5-59.   DOI   ScienceOn
28 Kiho T, Ukai S. Tochukaso (Semitake and others), Cordyceps species. Food Rev Int 1995;11:231-4.   DOI   ScienceOn
29 Sasaki F, Miyamoto T, Tamai Y, Yajima T. Optimum temperature and pH for mycelial growth of Cordyceps nutans Pat. (Ascomycetes). Int J Med Mushrooms 2005;7:301-4.   DOI