• The Plant Pathology Journal
• /
• 제23권2호
• /
• pp.37-44
• /
• 2007

Colletotrichum contains many important pathogens which cause economically significant diseases of crops like pepper, strawberry, tomato and apple. Forty four isolates were collected to characterize the diversity of Colletotrichum causing apple anthracnose in various regions of Korea. They were analyzed by random amplified polymorphic DNA (RAPD), internal transcribed spacer (ITS) of rDNA and partial $\beta$-tubulin gene DNA sequence, and culture characteristics on PDA and PDA-Benomyl. From the results of molecular analyses, 31 strains belonged to Colletotrichum gloeosporioides, ribosomal DNA group (RG) 4 of Moriwaki et al. (2002), 8 strains belonged to C. acutatum, A2 group of Talhinhas et al. (2005) and 5 strains to C. acutatum, A3 group of Talhinhas et al. (2005). Most isolates of C. gloeosporioides RG4 grew faster on PDA than strains of C. acutatum, A2 and A3 groups and most RG4 strains were sensitive to Benomyl. However, a few strains of RG4 grew slower and were resistant to Benomyl. On the basis of molecular characteristics, apple isolates of C. acutatum were clearly differentiated from red pepper isolates of the species, but apple isolates of C. gloeosporioides were not.

• 식물병연구
• /
• 제14권3호
• /
• pp.219-222
• /
• 2008

C. gloeosporioides와 C. acutatum의 개체군 밀도분석을 위해 기존 ITS부위를 이용한 PCR방법에 사용한 caInt2와 cgint 프라이머에 형광을 표지하여 C. acutatum에 특이적인 fcaInt2와 C. gloeosporioides에 특이적인 vcgint의 두 probe를 제작하였다. 이 두개의 프라이머와 Unicof1, Unicor1 primer를 이용 real time PCR을 수행하였을 때 C. acutatum은 fcaInt2 probe에, C. gloeosporioides는 vcgint에 특이적인 형광증폭곡선을 나타냄에 따라 delta Rn 값을 비교함으로 두 종의 구분이 가능하였다.

• The Plant Pathology Journal
• /
• 제33권2호
• /
• pp.118-124
• /
• 2017

Bell pepper is an economically important crop worldwide; however, production is restricted by a number of fungal diseases that cause significant yield loss. Chemical control is the most common approach adopted by growers to manage a number of these diseases. Monitoring for the development to resistance to fungicides in pathogenic fungal populations is central to devising integrated pest management strategies. Two fungal species, Fusarium incarnatum-equiseti species complex (FIESC) and Colletotrichum truncatum are important pathogens of bell pepper in Trinidad. This study was carried out to determine the sensitivity of 71 isolates belonging to these two fungal species to fungicides with different modes of action based on in vitro bioassays. There was no significant difference in log effective concentration required to achieve 50% colony growth inhibition ($LogEC_{50}$) values when field location and fungicide were considered for each species separately based on ANOVA analyses. However, the $LogEC_{50}$ value for the Aranguez-Antracol locationfungicide combination was almost twice the value for the Maloney/Macoya-Antracol location-fungicide combination regardless of fungal species. $LogEC_{50}$ values for Benomyl fungicide was also higher for C. truncatum isolates than for FIESC isolates and for any other fungicide. Cropping practices in these locations may explain the fungicide sensitivity data obtained.

• 한국식물병리학회지
• /
• 제2권1호
• /
• pp.31-36
• /
• 1986

구기자(Lycium chinense Mill) 탄저병은 구기자 열매에 수침상의 흑색 병반을 형성하고 포자층을 형성한다. 일반농가 포장에서의 발병율은 $0.8\%\~23\%$(평균 $9.6\%$)의 이병과율을 보였다. 구기자 탄저병균은 Colletotrichum gloeosporioides Penz와 Colletotrichum dematium(Fr.) Grove의 2종으로 동정되었다. 구기자에 대한 병원성은 공시한 C. gloeosporioides와 C. dematium 모두 어린 잎이나 줄기에는 병원성이 없었고 열매에는 강한 병원성을 나타냈다. 성숙과에는 C. dematium Isolate I이 특히 병원성이 강하였고 미숙과에 대하여는 C. dematium Isolate II만이 약하게 나타났으나 10일정도 경과하면 병원성 정도의 구분이 어려웠다.

• 한국균학회지
• /
• 제46권2호
• /
• pp.186-192
• /
• 2018

PCR 기술을 이용하여 고추와 토마토의 탄저병을 일으키는 Colletotrichum coccodes 균을 빠르고 정확하게 검출하는 방법을 개발하였다. Colletotrichum 13종 22개 균주로 부터 translation elongation factor 1 alpha 유전자를 분석하여 C. coccodes에 특이적인 coccoTef-F/cocco Tef-R primer set를 제작하였다. 제작된 primer를 사용한 결과 일반 PCR 방법으로 10 ng, real-time PCR 방법으로는 10 pg 수준에서 C. coccodes가 특이적으로 검출이 가능하였다. 인공적으로 C. coccodes에 감염시킨 고추와 토마토 종자에서도 일반 PCR 방법과 real-time PCR 방법 모두 C. coccodes검출이 가능하였다. 본 연구에서 개발한 PCR 방법은 수출입되는 종자에서 신속하고 정확하게 탄저병균 C. coccodes를 특이적으로 검출하는데 활용될 수 있을 것이다.

• Mycobiology
• /
• 제37권2호
• /
• pp.77-81
• /
• 2009

This study was conducted to identify the Colletotrichum species causing anthracnose disease of strawberry in Balgladesh and to evaluate in vitro activity of commercial fungicides it. Based on morphological and cultural characteristics, all 22 isolates were identified as Colletotrichum gloeosporioides. They developed white or glittery colonies with grey to dark grey reverse colony colors and they produced cylindrical conidia. The efficacy of five commercial fungicides, Bavistin DF, Dithane M-45, Sulcox 50 WP, Corzim 50 WP and Rovral 50 WP, were tested against the fungus. Bavistin inhibited radial growth completely and was followed in efficacy by Dithane M-45. In Bavistin DF treated media, the fungus did not produce conidia. The percent inhibition of radial growth of the fungus was increased with the increasing concentrations of fungicide.

• 한국균학회지
• /
• 제52권2호
• /
• pp.109-114
• /
• 2024

Anthracnose fruit rot (AFR) has been observed in greenhouses during the harvesting period in the Chungnam Province of South Korea. Fruits infected inside the greenhouse show black or brown spots, orange conidial masses and in some areas of the infected parts, white fungal growths are visible. The size of these spots gradually expands, leading to the necrosis of the fruits and flowers. Three isolates were obtained from infected fruits and identified as strains of the Colletotrichum acutatum species complex based on morphological characteristics. Multilocus sequence analysis of actin, chitin synthase, glyceraldehyde-3-phosphate dehydrogenase genes, and internal transcribed spacer rDNA regions revealed that the isolates belong to a monophyletic group with the type strain of C. nymphaeae. This is the first time C. nymphaeae has been confirmed in strawberry fruit in Korea.

• Mycobiology
• /
• 제29권3호
• /
• pp.142-144
• /
• 2001

The tetrahydropahnatine alkaloid was assayed against spore germination of some saprophytic and pathogenic fungi e.g., Alternaria solani, A. brassicicola, A. brassicae, A. alternata, Erysiphe pisi, Curvularia lunata, C. pallescens, C. maculans, Curvularia species, Colletotrichum species, C. musae, Helminthosporium echinoclova, H. pennisetti, H. spiciferum, and Heterosporium sp. It inhibite spore germination of all the fungi tested. Colletotrichum spp. Curvularia lunata, Helminthosporium spiciferum and Heterosporium sp. were most sensitive as complete inhibition of spore germination was observed at very low concentration(200 ppm).

• The Plant Pathology Journal
• /
• 제24권3호
• /
• pp.269-278
• /
• 2008

Ripe rot was frequently observed on fruits, leaves and stems of grape growing in eight locations in Korea from 2004 to 2006. All 30 isolates of Colletotrichum sp. were obtained from lesions of the ripe rot on grape plants. Out of the isolates, 19 isolates were identified as Colletotrichum acutatum and the others as Colletotrichum gloeosporioides based on morphological and cultural characteristics. Inter and intra specific variations of the Colletotrichum spp. isolates were investigated using RAPD and sequences of rDNA ITS and $\beta$-tubulin-2. Isolates of C. acutatum and C. gloeosporioides were distinctly differentiated by molecular analyses. Phylogenetic trees of ITS and$\beta$-tubulin-2 showed that Korean isolates of C. acutatum were clustered into groups A2 and A3 among the eight global groups. A2 included non-chromogenic isolates and A3 chromogenic ones. Both C. acutatum and C. gloeosporioides isolates were tested for pathogenicity to grape leaves. All isolates tested induced lesions on the leaves of grape by artificial inoculation. There was no difference in pathogenicity between C. acutatum and C. gloeosporioides isolates. This is the first report that C. acutatum except C. gloeosporioides causes grape ripe rot in Korea.

• 한국균학회지
• /
• 제50권3호
• /
• pp.235-241
• /
• 2022

Anthracnose crown rot (ACR) has been observed in greenhouses during the nursery and harvest seasons in Gangwon Province, Korea. Infected plants showed black leaf spot, dark sunken pink conidial masses on petioles, wilting, and eventually death. Five isolates were obtained from the lesions of strawberry plants and were identified as a Colletotrichum gloeosporioides species complex based on their cultural and morphological characteristics. Multilocus sequence analysis of actin, calmodulin, chitin synthase, glyceraldehyde-3-phophate dehydrogenase genes, and internal transcribed spacer rDNA regions showed that the isolates formed a monophyletic group with the type strain of C. siamense. Pathogenicity tests were performed on the isolate, and Koch's postulates were performed to verify the relationship between Colletotrichum sp. and the strawberry plant variety Seolhyang. The isolate was pathogenic to strawberry plants, which exhibited typical ACR symptoms. Based on morphological characteristics, pathogenicity, and DNA sequence analyses, the fungus isolated in Korea was identified as C. siamense. This is first time C. siamense has been confirmed in ever-bearing strawberry varieties in Korea.