• 한국응용곤충학회지
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• 제53권2호
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• pp.157-169
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• 2014

단감 병해충의 관리와 원활한 수출 정보를 위하여 2010년부터 2012년까지 3년 동안 전남 순천, 경남 진주, 창원(동읍과 북면), 김해, 그리고 울산 울주지역의 수출과원과 비수출과원과 수확과에서 병해충을 조사하였다. 수출 단감과원에서 확인된 병은 모무늬낙엽병, 탄저병, 둥근무늬낙엽병, 흰가루병, 잿빛곰팡이병이었고, 그 중 둥근무늬낙엽병의 피해가 높은 편이었다. 과일에서는 Colletotrichum gloeosporioides와 Colletotrichum acutatum 탄저병의 피해가 문제였다. 발견된 해충은 5목 20과 32속 33종이었고 점박이응애도 발생하였다. 그 중 애무늬고리장님 노린재와 식나무깍지벌레, 애모무늬잎말이나방이 비교적 넓은 지역에서 발생하고 있었고, 담배거세미나방, 차잎말이나방 등도 발생하고 있었다. 해충에 의한 피해는 매우 낮았다. 검역대상 해충인 복숭아명나방과 감꼭지나방은 거의 발생하지 않았거나 문제가 되지 않았고, 감나무애응애, 온실가루깍지벌레는 전혀 발견되지 않았다. 선과장과 수출과에서는 이들 검역대상 해충인 복숭아명나방, 감꼭지나방, 감나무애응애, 온실가루깍지벌레는 전혀 발견되지 않았고, 깍지벌레와 점박이응애가 낮은 비율로 발견되었다. 병으로는 진주와 김해의 저장고 수확과에서 C. acutatum탄저병 이병과가 1개 과에서만 발견되었다.

• The Plant Pathology Journal
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• 제31권3호
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• pp.316-321
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• 2015

A small cationic peptide (JH8944) was tested for activity against a number of pathogens of agricultural crops. JH8944 inhibited conidium growth in most of the tested plant pathogens with a dose of $50{\mu}g/ml$, although one isolate of Fusarium oxysporum was inhibited at $5{\mu}g/ml$ of JH8944. Most conidia of Fusarium graminearum were killed within 6 hours of treatment with $50{\mu}g/ml$ of JH8944. Germinating F. graminearum conidia required $238{\mu}g/ml$ of JH8944 for 90% growth inhibition. The peptide did not cause any damage to tissues surrounding maize leaf punctures when tested at a higher concentration of $250{\mu}g/ml$ even after 3 days. Liposomes consisting of phosphatidylglycerol were susceptible to leakage after treatment with 25 and $50{\mu}g/ml$ of JH8944. These experiments suggest this peptide destroys fungal membrane integrity and could be utilized for control of crop fungal pathogens.

• The Plant Pathology Journal
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• 제16권4호
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• pp.189-199
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• 2000

To search for the antifungal substances, various actino-mycete isolates were obtained from various soils of Korea using plate dilution method on the humic acid vitamin agar plates. In the screening procedures using a dual culture method, 32 actionomycete isolates were selected, which showed the inhibitory activity against mycelial growth of plant pathogenic fungi Altirnaria mali, Colletotrichum gloeosporides, Fusarium oxysporum f.sp. cucumerinum, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. Bioassay of the crude extracts from culture filtrates and mycelial mets revealed that 12 antagonistic actionomycetes produced highly active antifungal substances. Actinomycete strain S5-55 which showed the substantial antifungal activity against the tested fungi was selected for production of the antifungal substances. Based on the cytochemical and morphological characteristics, strain S5-55 was identified as a Streptomyces species. The results of the numerical identification using the TAXON program confirmed that Streptomyces strain S5-55 was identical with Streptomyces humidus including in TAXON major cluster 19. The production of antifungal substance was most favorable when S. humidus strain S5-55 was cultivated for 10 dats on soluble starch broth supplemented with $K_2$HPO$_4$. The antifungal substances active against the plant pathogenic fungi P. capsici and M. grisea were partially purified using $\textrm{C}_{18}$ reversed-phase column chromatography.

• The Plant Pathology Journal
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• 제26권3호
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• pp.231-237
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• 2010

The heat-inactivated (at $121^{\circ}C$ for 20 min) conidial suspension of Bipolaris cactivora (HICS) was evaluated for the control of Bipolaris stem rot of cactus caused by B. cactivora. Severe rot symptoms were developed on the cactus stem discs inoculated with B. cactivora from 5 days after inoculation. However, only small brownish spots developed on the stem discs treated with HICS 2 days prior to the pathogen inoculation. HICS also reduced symptom development on cactus stem discs inoculated with other fungal pathogens such as Alternaria alternata, Colletotrichum gloeosporioides, and Fusarium oxysporum, suggesting its disease-inhibitory efficacy may not be pathogen-specific. HICS significantly reduced severities of the stem rot disease on several cactus species including Hylocereus trigonus, Cereus peruvianus, Chamaecereus silvestrii and Gymnocalycium mianovichii, but not on Cereus tetragonus. Extensive wound periderms were formed in the stem tissues of inoculation and/or wounding sites on C. peruvianus treated with HICS alone or prior to the pathogen inoculation, but not on C. tetragonus, indicating the structural modifications may be related to the mechanism of disease suppression by HICS. HICS also reduced the disease development on the grafted cactus (H. trigonus stock and G. mianovichii scion) with the control efficacy nearly equivalent to the application of a commercial fungicide. All of these results suggest HICS can be used as an environmental-friendly agent for the control of the cactus stem disease.

• 농업과학연구
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• 제9권1호
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• pp.275-283
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• 1982

우리나라 주요작물(主要作物)의 종자전염균(種子傳染菌)에 대(對)한 기초자료(基礎資料)를 얻기 위하여 벼, 콩, 보리, 참깨와 시판중(市販中)인 12종(種)의 주요채소류(主要菜蔬類)에서 모두 144개(個)의 종자시료(種子試料)를 수집(收集)하여 종자건전검사(種子健全檢査)를 하였던 바 다음과 같은 결과(結果)를 얻었다. (1) 벼, 콩, 보리, 종자(種子)에서는 각각(各各) 18종(種), 15(種), 7(種)의 진균(眞菌)이 검출(檢出)되었다. 벼의 변색종자(變色種子)에서는 Epicoccum purpurascens, Pyricularia oryzae, Trichoconiella padwickii가 많이 검출(檢出)되었고, 콩종자(種子)에서는 탄저병균(炭疽病菌)인 Macrophomina phaseolina가 우리나라의 콩에서는 처음으로 검출(檢出)되었다. (2) 참깨종자(種子)에서는 12종(種)의 진균(眞菌)이 검출(檢出)되었으며 그 중(中) Alternaria속(屬)과 Corynespora cassiicola가 많이 검출(檢出)되었다. (3) 12종(種)의 시판(市販) 채소종자(菜蔬種子)에서는 모두 21종(種)의 진균(眞菌)이 검출(檢出)되었는데 특(特)히 Alternaria속(屬), Colletotrichum속(屬)과 Fusarium속(屬)이 많이 검출(檢出)되었다. 공시(供試)한 시판(市販) 채소종자(菜蔬種子)의 시료중(試料中)에는 각종(各種) 병원균(病原菌)에 심(甚)하게 오염(汚染)되어 낮은 발아율(發芽率)과 유묘감염(幼苗感染)을 일으키는 것이 있었다.

• 농업과학연구
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• 제47권4호
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• pp.1011-1020
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• 2020

Efficient management systems of facilities make it possible to manage environmental conditions properly, such as the temperature, humidity and light source required for the best growth of the crops, as well as for the mass production of fruit and vegetables with high quality every year through an advanced and protected cultivation system. Powdery mildew is a type of chronic disease that is difficult to control during the production of Korean melons under a protected cultivation system, the use of which is increasing in production areas in Korea. Two Bacillus strains isolated from soil samples showed antagonistic activities against several pathogens, specifically Botrytis cinerea, Colletotrichum gloeosporioides, and Fusarium oxysporum f.sp. melonis; they were identified as Bacillus velezensis M2 and B. amyloliquefaciens M3 in a molecular biological test of the nucleotide sequences of gyrase subunit A (gyrA). The treatment was given three times at intervals of five days with 400-fold diluted cultures of B. velezensis M2 and B. amyloliquefaciens M3. This led to the inhibition of the incidence of powdery mildew disease in Korean melon leaves, which resulted in effective control efficiency against the incidence of powdery mildew disease with control values of 87% and 65%, respectively. Cultures of antagonistic microbes tested in this study can be used to increase the efficiency as part of an environmentally friendly management scheme to prevent powdery mildew disease during the protected cultivation of crops, including Korean melons.

• 한국유기농업학회지
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• 제25권2호
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• pp.295-310
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• 2017

본 연구는 자생식물 참나무과(Fagaceae)인 붉가시나무, 참가시나무, 종가시나무, 개가시나무, 구실잣밤나무 5종을 이용하여 친환경 농자재로 개발을 위한 기초자료를 제공하고자 수용성 추출액 농도에 따른 Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici 등 5종의 작물 병원균에 하여 생장을 조사하였다. 공여체식물별에 따른 수용성 추출액 농도가 증가됨에 따라 작물 병원균 균사의 생장이 감소하는 경향을 보이나 공여체식물과 병원균의 종류에 따라 억제의 정도차이를 보였다. 구실잣밤나무의 수용성 추출액인 경우 Diaporthe citri, Phytophthora capsici, Pythium graminicola에 대하여 25% 처리구에서 약 84%의 균사 생장을 억제하였고 50% 이상 처리구에서는 87%의 균사 생장을 억제하는 것으로 분석되었다. Diaporthe citri의 경우 붉가시나무 수용성 추출액에서는 균사의 생장을 억제하지 못하는 것으로 분석되었다. 참가시나무, 종가시나무, 개가시나무는 15% 내외로 미비하게 균사생장을 억제 되었다. 항균활성을 보이는 수용체식물의 총 페놀 함량은 구실잣밤나무 22.32 mg/g, 참가시나무 8.32 mg/g, 종가시나무 6.83 mg/g, 개가시나무 5.95 mg/g, 붉가시나무 5.24 mg/g 순으로 분석되었으며, 참나무과 5종 중 구실잣밤나무의 수용성 추출액이 가장 항균력이 우수한 것으로 판단된다.

• 한국자원식물학회지
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• 제30권4호
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• pp.343-351
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• 2017

401종의 식물에서 얻은 메탄올 추출물 662개를 식물 병원성 곰팡이[딸기잿빛곰팡이병균(Botrytis sp.), 고추탄저병균(Colletotrichum sp.), 작약점무늬병균(Alternaria sp.) 및 작약검은뿌리썩음병균(Cylindrocarpon sp.)]에 대한 분생포자의 발아억제 활성을 microdilution assay로 측정하였다. 검정에 사용한 추출물 중에서 36종의 추출물이 1종 이상의 곰팡이에 대하여 억제활성을 보였다. 짚신나물, 고본, 족도리풀, 일천궁, 백선 및 목향의 뿌리, 능소화의 잎가지, 감초의 지상부, 뽕나무의 가지 추출물은 식물의 지상부 조직에서 발병하는 균(Botrytis sp., Colletotrichum sp. 그리고 Alternaria sp.)과 뿌리에 발병하는 균(Cylindrocarpon sp.)의 포자발아를 모두 억제하는 항균활성을 보여 주어 이들 추출물의 항균범위가 넓은 것으로 나타났다. 각각의 곰팡이 대한 추출물의 억제활성을 보면, 뽕나무의 가지와 고삼의 뿌리 추출물은 1,250 mg/L에서 딸기잿빛곰팡이병균의 포자발아를 완전히 억제하였다. 고추탄저병균의 포자발아억제에 가장 강한 추출물은 홀아비꽃대의 뿌리였으며, $1,250{\mu}g/ml$에서 완전히 억제하였다. 작약점무늬병균에 가장 강한 억제활성을 나타낸 추출물은 감초의 지상부였으며, $625{\mu}g/ml$에서 포자의 발아를 100% 억제하였고, 새모래덩굴의 뿌리와 뽕나무 가지 추출물은 $1,250{\mu}g/ml$에서 억제하였다. 작약검은뿌리썩음병균에 가장 강한 억제활성을 나타낸 추출물은 감초의 지상부와 목향의 뿌리였으며, $1,250{\mu}g/ml$에서 포자발아를 억제하였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.95.2-96
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• 2003

In order to develop a new microbial fungicide for the control of vegetable diseases using endophytic bacteria, a total of 260 bacterial strains were isolated from fresh tissues of 5 plant species. After they were cultured in broth media, their antifungal activities were screened by in vivo bioassays against Botrytis cinerea(tomato gray mold), Pythium ultimum(cucumber damping-off), Phytopkhora infestans(tomato late blight), Colletotrichum orbiculare(cucumber anthracnose), and Blumeria graminis f. sp. hordei(barley powdery mildew). As the results of screening, 38 bacterial strains showed potent antifungal activities against at least one of 5 plant pathogens. A bacterial strain EB072 displayed potent disease control activities against 3 plant diseases. Among the bacterial strains with a potent antifungal activity against cucunlber anthracnose, three bacterial strains, EB054, EB151 and EB215, also displayed a potent in vitro antifungal activity against C. acutatum, a fungal agent causing pepper anthracnose. A bacterial strain EB215 obtained from roots of cucumber was identified as Burkholderia cepacia based on its physiological and biochemical characteristics and 165 rRNA gene sequence. An antifungal substance was isolated from the liquid cultures of B. cepacia EB215 strain by ethyl acetate partitioning, repeated silica gel column chromatography, and invitro bioassay, Its structural determination is in progress by various instrumental analyses.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.78.1-78
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• 2003

An osmotin-like protein (CAOSMl) gene was isolated from pepper leaves infected with the avirulent strain Bv5-4a of Xmthomonas campestris pv. vesicatoria. The cDNA encodes a polypeptide of 250 amino acids with a molecular mass of 27, 361 Da. Its amino acid sequence is highly homologous to various osmotin-like proteins from other plant species. The CAOSMl gene expression was organ- and tissue-specifically regulated In pepper plants. The CAOSMl mRNA was intensely localized in the endodermis area of root tissue and in the phloem cells of vascular bundles of red fruit tissue, but not in leaf, stem, and green fruit tissues of healthy pepper plants. Infection by X. c. pv vesintoria, Colletotrichum coccodes, or Phytopkhora capsici iinduced CAOSMl transcription in the leaf or stem tissues. Expression of the CAOSMl gene was somewhat higher in the incompatible than the compatible interactions of pathogens with pepper. The CAOSMl mRNA was prevalently localized in the phloem cells of the vascular bundle of leaf tissues infected by C. coccodes. The CAOSMl gene was activated in leaf tissues by treatment with ethylene, methyl jasmonate, high salinity, cold acclimation and mechanical wounding, but not by abscisic acid (ABA) and drought. These results indicate that the pepper CAOSMl protein functions in response to Pathogens and some abiotic stresses in pepper plants