• Mycobiology
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• v.45 no.1
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• pp.1-8
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• 2017

Despite the huge worldwide diversity of Trichoderma (Hypocreaceae, Ascomycota), only about 22 species have been reported in Korea. Thus, between 2013 and 2015, soil-derived Trichoderma spp. were isolated to reveal the diversity of Korean Trichoderma. Phylogenetic analysis of translation elongation factor 1 alpha gene was used for identification. Among the soil-derived Trichoderma, Trichoderma albolutescens, T. asperelloides, T. orientale, T. spirale, and T. tomentosum have not been previously reported in Korea. Thus, we report the five Trichoderma species as new in Korea with morphological descriptions and images.

• Journal of Veterinary Science
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• v.23 no.1
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• pp.3.1-3.12
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• 2022

Background: Domestic yaks are the most important livestock species on the Qinghai-Tibetan Plateau. Adult female yaks normally breed in the warm season (July to September) and enter anestrous in the cold season (November to April). Nevertheless, it is unclear how ovarian activity is regulated at the molecular level. Objectives: The peculiarities of yak reproduction were assessed to explore the molecular mechanism of postpartum anestrus ovaries in yaks after pregnancy and parturition. Methods: Sixty female yaks with calves were observed under natural grazing in Haiyan County, Qinghai Province. Three yak ovaries in pregnancy and postpartum anestrus were collected. RNA sequencing and quantitative proteomics were employed to analyze the pregnancy and postpartum ovaries after hypothermia to identify the genes and proteins related to the postpartum ovarian cycle. Results: The results revealed 841 differentially expressed genes during the postpartum hypoestrus cycle; 347 were up-regulated and 494 genes were down-regulated. Fifty-seven differential proteins were screened: 38 were up-regulated and 19 were down-regulated. The differential genes and proteins were related to the yak reproduction process, rhythm process, progesterone-mediated oocyte maturation, PI3K/AKT signaling pathway, and MAPK signaling pathway categories. Conclusions: Transcriptome and proteomic sequencing approaches were used to investigate postpartum anestrus and pregnancy ovaries in yaks. The results confirmed that BHLHE40, SF1IX1, FBPX1, HSPCA, LHCGR, BMP15, and ET-1R could affect postpartum hypoestrus and control the state of estrus.

• BMB Reports
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• v.40 no.4
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• pp.595-603
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• 2007

To investigate whether phospholipase D (PLD, EC 3.1.4.4) plays a role in adaptive response of post-harvest fruit to environment, a PLD gene was firstly cloned from grape berry (Vitis Vinifera L. cv. Chardonnay) using RT-PCR and 3'- and 5'-RACE. The deduced amino acid sequence (809 residues) showed 84.7% identity with that of PLD from Ricinus communis. The secondary structures of this protein showed the characteristic C2 domain and two active sites of a phospholipid-metabolizing enzyme. The PLD activity and its expression in response to heat acclimation were then assayed. The results indicated PLD was significantly activated at enzyme activity, as well as accumulation of PLD mRNA and synthesis of new PLD protein during the early of heat acclimation, primary suggesting that the grape berry PLD may be involved in the heat response in post-harvest grape berry. This work offers an important basis for further investigating the mechanism of post-harvest fruit adaptation to environmental stresses.

• Biomedical Science Letters
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• v.22 no.4
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• pp.127-139
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• 2016

A species of the fungal genus Cordyceps has been used as an ingredient of traditional Chinese medicine. In this study, we prepared cordycepin-enriched WIB-801CE, an ethanol extract from culture solution of Cordyceps militaris-hypha, and evaluated its antiplatelet effects on human platelet aggregation. WIB-801CE dose-dependently inhibited ADP-, collagen-, and thrombin-induced platelet aggregation. These antiplatelet effects by WIB-801CE were associated with the attenuation of thromboxane $A_2$ ($TXA_2$) production and serotonin release by ADP, collagen, and thrombin. The inhibition of $TXA_2$ production by WIB-801CE was due to the inhibition of cyclooxygenase-1, $TXA_2$ synthase, and cytosolic phospholipase $A_2$ activity. Therefore, these data suggest that WIB-801CE may be a beneficial component against protection from platelet aggregation-mediated thrombotic disease.

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.52.1-52.1
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• 2008

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.1
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• pp.159-164
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• 2015

Color is distinguished due to the light in which natural light is reflected by object and made with combination of RGB(red, green, blue; three colors). This study proposes color analysis system with optical method to be used conveniently. Color information of sample is determined with the optical sensor. By using the CIE diagram in particular, it detects purity value and wavelength. The method to distinguish color is very economical, simple, and convenient. The result can be used to confirm accurate information of color for various applications.

• Fisheries and Aquatic Sciences
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• v.25 no.2
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• pp.64-75
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• 2022

This study aimed to investigate the effect of different coating materials on the quality of shrimp (Litopenaeus vannamei) during chilled storage for 10 days. Fresh shrimp were randomly divided into five groups: the control group, the hypotaurine treatment group (2%), the chitosan group (1%), the hypotaurine + chitosan group (2% hypotaurine solution with 1% of chitosan), and the sodium metabisulfite treatment group (1.25%). Compared with other treatments, the lower accumulation of total visible counts (TVC, 5.25 Log₁₀ CFU/g), total volatile basic nitrogen (TVB-N, 22.5 mg/100 g) and thiobarbituric acid values (TBA, 0.58 mg MDA/kg) suggested that coating of chitosan-hypotaurine could retard the microbial activity, protein degradation and lipid oxidation of shrimp. Meanwhile, results demonstrated that the chitosan coating combined with hypotaurine showed an excellent performance in inhibiting quality deterioration (pH 7.5, ∆E 7.0, hardness 393 g, and elasticity 0.69). Furthermore, the melanosis degree of shrimp was alleviated, and the sensory parameters, including appearance, odor and texture, were maintained to the acceptable level by chitosan based hypotaurine treatment during the chilled storage.

• Proceedings of the Korean Information Science Society Conference
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• 2010.06b
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• pp.107-111
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• 2010

급변화하는 비즈니스 환경에 가장 빠르게 대응할 수 있고, IT 자원의 상호운용성 및 재활용성을 증대할 수 있는 SOA 기반 소프트웨어 시장이 급격히 증가함에 따라 SOA 기반 시스템 품질 평가 방법들이 연구되고 있다. 그러나, SOA에 융합된 BI 관점의 평가 방법은 연구가 부족하다. 본 연구에서는 ISO/IEC 9126국제 표준을 참조하여 SOA와 융합된 BI의 품질 평가 특성을 제시하고, 사례 연구를 통해 품질 평가 방법을 제안 및 검증한다. 본 연구를 통해, BI를 SOA와 융합하는 과정에 품질 특성을 고려하여 구현 및 융합함으로써 소프트웨어의 품질 향상을 유도하고 완성된 소프트웨어의 품질 평가에 활용될 것으로 기대한다.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.63 no.8
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• pp.1121-1127
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• 2014

Many studies have been done to measure and analyze color for various purposes. Visual assessment has lack of objectivity and the equipment for color measurement is very expensive. In this study, we developed a device for quantitative analysis of the color using optical elements. With the color sensor, the ratio of RGB was calculated by measuring the light intensity that is reflected from an object. Inverse transformation of optical signal was performed to detect the color density. The suggested color analyzer can detect color information as well as sample concentration. Results of this study are expected to be used in various medical fields such as pH indicator and urine analysis.

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.259.2-259.2
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• 2008