• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.15-22
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• 2008

Four series (S, M, R, and W) of Alternaria longipes isolates were obtained based on consecutive selection with Dimethachlon (Dim) and ultraviolet irradiation. These isolates were then characterized according to their tolerance to Dim, sensitivity to osmotic stress, and phenotypic properties. All the selected Dim-resistant isolates showed a higher osmosensitivity than the parental strains, and the last generation was more resistant than the first generation in the M, R, and W series. In addition, the changes in the Dim resistance and osmotic sensitivity were not found to be directly correlated, and no distinct morphologic characteristics were found among the resistant and sensitive isolates, with the exception of the resistant isolate K-11. Thus, to investigate the molecular basis of the fungicide resistance, a group III two-component histidine kinase (HK) gene, AlHK1, was cloned from nineteen A. longipes isolates. AlHK1p was found to be comprised of a six 92-amino-acid repeat domain (AARD), HK domain, and response regulator domain, similar to the Os-1p from Neurospora crassa. A comparison of the nucleotide sequences of the AlHK1 gene from the Dim-sensitive and -resistant isolates revealed that all the resistant isolates contained a single-point mutation in the AARD of AlHK1p, with the exception of isolate K-11, where the AlHK1p contained a deletion of 107 amino acids. Moreover, the AlHK1p mutations in the isolates of each respective series involved the same amino acid substitution at the same site, although the resistance levels differed significantly in each series. Therefore, these findings suggested that a mutation in the AARD of AlHK1p was not the sole factor responsible for A. longipes resistance to dicarboximide fungicides.

• 동의생리병리학회지
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• 제28권6호
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• pp.674-682
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• 2014

The purpose of this study was to identify the effect of applying oriental music therapy and reliability of electroencephalogram(EEG) equipment. The study was approved by the critical trial judge committee from ${\bigcirc}{\bigcirc}$(IRB No. 2013-07) university. In order to measure test-retest reliability for 15 subjects, EEG for same participants were measured using same method mentioned above after 2 hours from the first measurement. Same provider implemented to each person at same time. Firstly, EEG was measured for 5 minutes after the subject with attached electrodes sat on chair comfortably for 10 minutes. Then, the subject was given mental stress using the four fundamental arithmetic operations for 5 minutes, and measured EEG for another 5 minutes. After that the subject sat on the chair comfortably listening oriental medicine music therapy for 5 minutes, and EEG was measured for 5 minutes again. There was no side effect regarding music therapy reported. Raw data, which was measured in each step, were converted through FFT(fast fourier transform) and analyzed after divided into certain frequency including ${\alpha}$ wave, ${\beta}$ wave, ${\theta}$ wave, immersion wave, stabilization wave, sef100 wave, and sef95 wave. Data were analyzed using wilcoxon signed rank test, Intraclass correlation coefficient(ICC), repeated measures ANOVA with the SPSS program. In test-retes method, there were significantly differences in ${\alpha}$ wave, ${\beta}$ wave, immersion wave, stability wave, ${\theta}$ wave, sef100 wave, sef95 wave. ICC has shown a high degree of reliability that it was ${\alpha}$ wave .877, ${\beta}$ wave .855, ${\gamma}$ wave .895, immersion wave .897, stability wave .816, ${\theta}$ wave .904, sef100 wave .910, sef95 wave .776. Also, there was a statistically significant difference in ${\alpha}$ wave after applying oriental music therapy. Based on these results, it is considered that average of the channel EEG and application of oriental music therapy would be practiced by increase of sample size using this machine.

• 생명과학회지
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• 제21권3호
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• pp.400-405
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• 2011

Autophagy는 항상성 유지와 스트레스반응을 효율적으로 조정하기 위해 필수적인 세포 내 질적 조절작용이다. 노화가 진행되는 동안 autopahgy에 의한 degradation 효율성 저하와 그로 인한 세포 내 부산물의 축적이 증가하여 결국, 근육의 약화를 초래한다. 그러므로 본 연구의 목적은 골격근에서 운동에 의한 autopahgy 관련 단백질의 변화를 규명하는데 있다. 이를 위해 24마리의 Young 그룹과 Old 그룹을 나누어 각각 대조군(n=6)과 운동군(n=6)으로 배정하였다. 운동은 8주간 주 5회 실시하였고, 트레드밀 속도 16.4 m/min와 경사도 4%로 설정하여 40분간 지속적인 운동을 실시하였다. autopahgy 관련 단백질에 대한 검증 결과 Young 그룹과 비교하여 Old 그룹에서 LC3-1, Beclin-1, Atg7은 모두 유의하게 감소하였다. 그러나 8주간의 규칙적인 운동에 의하여 autophagy 관련 단백질은 증가하는 것으로 나타났다. 따라서 노화에 의해 약화된 autopahgy 기능은 규칙적인 운동에 의해 개선될 수 있을 것으로 사료된다.

• KIEE International Transaction on Systems and Control
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• 제2D권2호
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• pp.78-91
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• 2002

In the thermal power plant, there are six manipulated variables: main steam flow, feedwater flow, fuel flow, air flow, spray flow, and gas recirculation flow. There are five controlled variables: generator output, main steam pressure, main steam temperature, exhaust gas density, and reheater steam temperature. Therefore, the thermal power plant control system is a multinput and output system. In the control system, the main steam temperature is typically regulated by the fuel flow rate and the spray flow rate, and the reheater steam temperature is regulated by the gas recirculation flow rate. However, strict control of the steam temperature must be maintained to avoid thermal stress. Maintaining the steam temperature can be difficult due to heating value variation to the fuel source, time delay changes in the main steam temperature versus changes in fuel flow rate, difficulty of control of the main steam temperature control and the reheater steam temperature control system owing to the dynamic response characteristics of changes in steam temperature and the reheater steam temperature, and the fluctuation of inner fluid water and steam flow rates during the load-following operation. Up to the present time, the Proportional-Integral-Derivative Controller has been used to operate this system. However, it is very difficult to achieve an optimal PID gain with no experience, since the gain of the PID controller has to be manually tuned by trial and error. This paper focuses on the characteristic comparison of the PID controller and the modified 2-DOF PID Controller (Two-Degrees-Freedom Proportional-Integral-Derivative) on the DCS (Distributed Control System). The method is to design an optimal controller that can be operated on the thermal generating plant in Seoul, Korea. The modified 2-DOF PID controller is designed to enable parameters to fit into the thermal plant during disturbances. To attain an optimal control method, transfer function and operating data from start-up, running, and stop procedures of the thermal plant have been acquired. Through this research, the stable range of a 2-DOF parameter for only this system could be found for the start-up procedure and this parameter could be used for the tuning problem. Also, this paper addressed whether an intelligent tuning method based on immune network algorithms can be used effectively in tuning these controllers.

• 치위생과학회지
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• 제11권6호
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• pp.563-571
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• 2011

본 연구는 울산지역에 위치하는 2개 대학의 학생들 330명을 대상으로 건강증진행위를 하는데 있어 중요한 변수로 인식된 기존의 변수들을 모아 구강건강증진 행위에 대한 경로를 분석한 결과 다음과 같은 결론을 얻었다. 1. 최종모형의 적합도는 ${\chi}^2$ 값은 39.06, 유의확률은 0.152, GFI= .982, AGFI= .948, NFI=.967, NNFI= .982, RMSR= .028로 나타나 자료와 잘 부합되는 것으로 나타났다. 2. 가설모형에서 선정된 39개의 경로중 27개의 경로가 통계적으로 직접효과와 총효과가 유의하였으며, 2개 경로는 간접효과와 총효과가 유의하여 총 27개의 경로가 유의한 것으로 나타났다. 3. 현재의 구강건강증진행위에 영향을 미치는 변수로는 과거구강건강경험(총효과=.805), 주관적 구강건강(총효과=.717), 지각된 장애성(총효과=-.226), 자기효능감(총효과=.179), 사회적지지(총효과=.192), 구강건강통제위(총효과=.120), 의도(총효과=.167)로 나타났다(P<.05). 4. 현재의 구강건강증진행위에 긍정적으로 영향을 미치는 변수로는 과거구강건강경험, 주관적 구강건강, 사회적지지, 자기효능감, 의도, 구강건강통제위의 순으로 긍정적인 영향을 미치는 것으로 나타났고, 지각된 장애성은 부정적인 의미로 영향을 미치는 것 으로 나타났다.

• 대한토목학회논문집
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• 제33권2호
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• pp.821-831
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• 2013

최근 연구결과 레일연마 및 장대레일화를 통해 누적통과톤수에 의한 레일교체기준의 연장이 가능한 것으로 보고되었다. 본 연구에서는 장기 사용레일에 대한 실내피로시험을 수행하였고, 파괴확률 50%에서의 장기 사용레일의 잔존수명을 표현한 S-N 선도는 적은 실험데이터에 대한 가중치 확률 해석기법을 사용하여 도출하였다. 여기서 피로시험에 사용된 레일들이 누적통과톤수가 서로 다르기 때문에 누적통과톤수를 평균하여 반복횟수를 수정하였다. 또한, 레일표면요철 및 열차속도를 고려한 레일 저부 휨응력은 기존 연구결과 도출된 레일휨응력 예측식을 사용하여 장기 사용레일의 잔존수명을 평가하였다. 따라서 본 연구를 통해 도시철도에서 레일연마를 통한 레일관리가 이루어진다면 누적통과톤수에 의한 레일교체기준(약 8억톤)보다 약 2억톤이상 연장 사용이 가능하며, 피로한도 이하의 응력범위에 대한 피로시험결과를 바탕으로 레일의 피로수명을 평가하기 위해서는 수정마이너법칙보다는 피로한도 이상에서의 S-N선도 기울기의 1/2인 하이바크법칙을 적용하는 것이 합리적이라 판단되었다.

• 한국직업건강간호학회지
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• 제13권2호
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• pp.104-117
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• 2004

Objectives: To propose the basic data for the development of the optimum health promotion program (HPP) for the workers in a petrochemical industry complex. Methods: The subjects were 553 workers who worked at the six plants in a petrochemical industry complex. From February to March 2003, questionnaire survey was conducted by the nurses with occupational health specialty about the life styles, interests, self-cognitions, practices of health behavior, subjective symptoms thought to be related the working environments, burden to the own health, needs and intentions to participation of the HPP. Data analysis was done using SPSS program (ver 10.0) with ${\alpha}=0.05$ of statistical significance. Results: The mean age and tenure of the subjects were 39.7 years and 14.2 years, respectively, and proportion of smokers was around 50%. Two of third (66%) satisfied their own job, and 58% recognized themselves healthy. The most frequent health behavior were exercise (60%) and regular health check (87%) in individual and job based, respectively. About half of them (58%) had at least one of the symptoms thought to be related the working environments, and the most frequent one was odor (21%). These symptoms were more frequent in case of the shorter career. The needs of HPP was relatively higher in exercise (66%), cancer prevention (51%), stress control (47%), and intention to participation was also the highest in exercise (64%). Job satisfaction was higher in case of the higher interests and cognitions to their own health, and thought that the ownership's interest to workers' health be high (p<0.01), but no associations with the subjective symptoms. The mean level of burden to the own health were $14.1{\pm}13.9$ of physical, $11.7{\pm}13.6$ of emotional, $9.1{\pm}10.7$ of behavioral as out of one hundred. They felt the more burden in case of office workers, the lower interests and cognitions to their own health, the lower satisfaction to their job, the frequent drinking group, and complained the subjective symptoms (p<0.05). Abnormal results of the regular health check was higher in the frequent drinking group (p=0.083). Conclusions: For the optimum HPP for the workers in the petrochemical complex, the ownership's interest to workers' health would be enhanced, and the priority would be focused on exercise and drinking habits control.

• 지역사회간호학회지
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• 제11권2호
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• pp.470-485
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• 2000

The purpose of this study was to investigate health promotion lifestyle profiles of the Korean elderly in Japan. The subjects of this study were 202 elders in Aichi prefecture. The method of this study questionnaires interviews, and a self-administered questionnaire from the 1st to the 20th of April in 2000. This study tool was based on Walker et al. (1987)'s Health Promotion lifestyle Profiles (HPLP). Analysis of the data used an SPSS/pc+ WIN 9.0 program. Data analyses were conducted by using frequency, percentage, t-test, ANOVA. Pearson correlation, Kendal tau and Stepwise Multiple Regression. The results were as follows: 1. The average score of Health Promotion Lifestyle Profiles of Korean elderly in Japan was 2.72(SD=0.40). On the sub-scale of Health Promotion Lifestyle Profiles: nutrition(M = 3. 06), self-actualization(M =2.99), interpersonal support (M=2.81), stress management (M=2.68) revealed higher scores, whereas the scores of health responsibility(M=2.52), and exercise(M=1.92) were lower. 2. Health Promotion Lifestyle Profiles were significantly different by age (F = 11. 02, p = .000), religion(F=2.96, p=.033), occupation(F=6.91, p=.000), living family status(F=13.07, p=.000), living family number(F=11.74, p=.000), monthly pocket money(F=18.79, p=.000), the source of pocket money (F=7.18, p=.000), and the length of residence(F=9.79, p=.000). 3. Health Promotion Lifestyle Profiles was significantly predicted by monthly pocket money($r^2$ change =0. 188, p=.000), sex($r^2$ change= 0.066, p= .000), the length of residence($r^2$ change = 0.059, p = .000), the source of pocket money($r^2$ change=0.036, p= .000), age ($r^2$ change=0.018, p=.000). These variables showed a positive correlation with health promotion lifestyle profiles of the Korean elderly in Japan. These variables explained 36.7% of the variance of Health Promotion Lifestyle Profiles. On the basis of the above findings, Health Promotion Lifestyle Profiles programs for Korean elderly in Japan needs be to developed with Japanese social-culture perspectives and needs to develop an intervention method to improve Health Promotion Lifestyle Profiles. It is also suggested that further research is required.

• 해양환경안전학회지
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• 제25권7호
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• pp.936-944
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• 2019

보이지 않는 힘으로도 불리는 잠수함은 수중에서 활동하는 은밀성을 장점으로 대함전, 대잠전 및 핵심표적 타격 등의 임무를 수행하는 전략 수중 무기 체계로 심해에서 높은 수압을 견디며 작전을 수행할 수 있어야 한다. 이러한 관점에서 잠수함 압력 선체는 잠항 깊이에 상응하는 외부 수압에 저항하는 가장 중요한 체계로서 누수, 화재, 충격 및 폭발과 같은 위험으로부터 안전성을 확보함으로써 생존성을 높임과 동시에, 작전 수행 능력을 유지할 수 있게 해주는 강도를 확보하고 있어야 한다. 이를 위해서는 잠수함 압력 선체의 구조형상 설계가 초기에 수행되는 것이 합리적이다. 특히, 함미 원추부 구조물과 압력선체 평형부 및 함미 비압력선체를 연결하는 함미 트랜지션 링의 경우, 설계된 잠수함에 따라 다양한 형상을 띄고 있다. 본 구조물 설계를 위해서는 응력 흐름과 연결성을 고려한 설계뿐만 아니라 복잡한 형상이 기인한 구조물 제작 투입 시수 증가로 인한 원가 상승 또한 검토해야 한다. 따라서, 본 연구에서는 4가지 서로 다른 형상을 갖는 함미 트랜지션 링에 대해서 비선형 유한요소해석을 통한 구조 강도 검토와 더불어 함미 트랜지션 링 형상 복잡도에 따른 작업 일수 및 자재비 검토를 통해 경제성 측면에서의 적정성 검토를 수행하였으며, 검토된 4가지 형상 중 가장 합리적인 잠수함 함미 트랜지션 링 형상을 제안하였다.

• 한국재난정보학회 논문집
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• 제17권4호
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• pp.747-754
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• 2021

연구목적: 곡선 교량은 기하하적 특성으로 직선교량에 비해 복잡한 거동을 보이기 때문에 지진 안전성 평가가 반드시 이루어져야 한다. 본 연구에서는 곡선 거더를 갖는 교량의 강재 재료 특성의 불확실성을 고려한 지진 취약도 평가를 수행하였다. 연구방법: I형 곡선 거더를 갖는 교량의 유한요소 모델을 구축하였으며 선행연구에서 제시된 강재 특성의 통계적 매개변수를 이용하였다. 라틴 하이퍼큐브 기법을 이용하여 100개의 강재 재료 모델을 샘플링하였다. 경주지진의 지반가속도를 0.2g, 0.5g, 0.8g, 1.2g, 1.5g로 scale을 변화시켜 지진 취약도 평가를 수행하였다. 연구결과: 곡선거더의 지진 취약도 평가결과 한계상태가 190MPa일 때 0.03g 파괴가 시작되었으며 한계상태가 315MPa일 때 0.11g를 초과하면서 파괴가 시작되는 것으로 나타났다. 결론: 본 연구에서는 재료 불확실성을 고려한 지진 취약도 평가를 수행하였으며 추후 연구에서는 지진파의 불확실성과 재료의 불확실성을 동시에 고려한 지진 취약도 분석이 필요할 것으로 판단된다.