• Journal of Aquaculture
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• v.15 no.2
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• pp.111-117
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• 2002

Effect of substrate composition, stocking density and food organisms on survival and growth of the surf clam, Spisula sachalinensis was studied. Spat collection from the substratum was the highest, when its sand size was less than 250 $\mu m4; at such substratum, survival and growth of the surf clam were also the highest. The spat (1.4 mm) grew to 4.6 mm and 8.2 mm in shell length on day 30 and 50, respectively. The relationship between shell length (SL) and shell height (SH), shell breadth (SB), total weight (TW) and shell weight (SW) of the spat are formulated by the following equations; SH : 0.7351SL+0.2272 ( $r^2$ =0.9600)i SB = 0.4037SL+0.2112 ($r^2$ = 0.9452); TW : 0.2824SL^{2.7020} ($r^2$ = 0.9820)i SW = $0.4021SL^{2.0217} ($r^2$ : 0.6169). In the substratum containing fine sand, growth and survival of the clam roared at the density of 1~2 ind./$cm^2$ were optimal. The quality of feed decreases in the following order; C. calcitrans, mixture group, I. galbana, P. lutheri, T. suecica.

• Korean Journal of Food Science and Technology
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• v.34 no.6
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• pp.1130-1133
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• 2002

Domoic acid, and amnesic shellfish poison, is a neurotoxin frequently found in shellfishes. Guidance level for the consumable shellfish has been established as $20\;{\mu}g$ domoic acid/g by Health and Welfare Canada and U.S. FDA. Domoic acid is produced by pennate diatom, a Nitzschia pungens f. multiseries ingested by the shellfish. Content of domoic acid in shellfish samples collected along the Korean shoreline from May to December of 1999 was analyzed. The collection included 1 Gastropoda (Murex shell) and 11 Bivalvias (oyster, little neck clam, orient hard clam, venus clam, surf clam, ark shell, hard-shelled mussel, pen shell, jack-knife clam, pink butterfly shell, and granulated ark shell). Samples were homogenized, extracted with 50% methanol, filtered, and analyzed by reversed-phase liquid chromatography at 242 nm with mobile phase consisting of 10% acetonitrile and 0.1% trifluoroacetic acid. Recovery of the HPLC analysis was 95.80% (${\pm}1.09$). All tested samples showed no domoic acid at the detection limit of 50 ng/g.