• Title/Summary/Keyword: Collection method

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Comparison of Three Different Methods for Campylobacter Isolation from Porcine Intestines

  • Shin, Eun-Ju;Lee, Yeon-Hee
    • Journal of Microbiology and Biotechnology
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    • v.19 no.7
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    • pp.647-650
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    • 2009
  • Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter isolates were obtained from 178 samples (89%) by direct streaking of colon mucosa, 142 samples (71%) by direct streaking of a swab, and 94 samples (47%) by pre-enrichment of intestinal contents in Preston broth. Direct streaking of colon mucosa was superior to the other two isolation methods, in terms of rapidity and higher efficiency. When isolates were identified with various biochemical tests and PCRs specific to 16s rRNA, mapA, and ceuE, C. coli was the predominant species (87%) in porcine, whereas the rest of the isolates were identified as C. lanienae.

Simulation of particle filtration by Brownian dynamics (Brownian dynamics 를 이용한 입자 포집 모사)

  • Bang, Jong-Geun;Yoon, Yoong-Sup
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1922-1927
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    • 2008
  • In the present study, deposition of discrete and small particles, which diameter is less than $1{\mu}m$, on a filter element was simulated by stochastic method. Trajectory of each particle was numerically solved by Langevin equation and Brownian random motion was treated by Brownian dynamics. Lattice Boltzmann method (LBM) was used to solve flow field around the filter collector and deposit layer. Interaction between flow field and deposit layer was obtained from a converged solution from an inner-loop calculation. Simulation method is properly validated and collection efficiency due to different filtration parameters are examined and discussed. Morphology of deposit layer and its evolution was visualized in terms of the particle size. The particle loaded effect on collection efficiency was also discussed.

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Numerical Study of Particle Collection and Entrainment in Electrostatic Precipitator (집진기내 입자 포집과 비산 문제에 대한 수치적 연구)

  • Kim, Ju-Hyeon;Kweon, Soon-Cheol;Kwon, Ki-Hwan;Lee, Sang-Hwan;Lee, Ju-Hee
    • The KSFM Journal of Fluid Machinery
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    • v.15 no.1
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    • pp.27-35
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    • 2012
  • A numerical simulation for particle collection efficiency in a wire-plate electrostatic precipitator (ESP) has been performed. Method of characteristics and finite differencing method (MOC-FDM) were employed to obtain electric field and space charge density, and lattice boltzmann method (LBM) was used to predict the Electrohydrodynamic (EHD) flow according to the ion convection. Large eddy simulation (LES) was considered for turbulent flow and particle simulation was performed by discrete element method (DEM) which considered field charging, electric force, drag force and wall-collision. One way coupling from FDM to LBM was used with small and low density particle assumption. When the charged particle collided with the collecting plate, particle-wall collision was calculated for re-entertainment effect and the effect of gravity force was considered.

The Current Control Methods Comparison of Boost Converter for Unity Power Factor (단위 역률 구현을 위한 부스트 컨버터의 전류제어방식 비교)

  • 최재동;성세진
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.12 no.3
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    • pp.67-73
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    • 1998
  • In this paper, the BPFC(Boost Power Factor Collection) method is proposed to improve power factor and harmonic distortion. This method not only reduces the current and voltage distortion but also the physical size and brings expected high efficiency through current control methods. And the proposed BPFC has a distinct difference by control methods. The BPFC method proposed in this study has the characteristic of the high power factor and low harmonic distortion, and the control method using the peak current control and PWM average current control is comparative.

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Data De-duplication and Recycling Technique in SSD-based Storage System for Increasing De-duplication Rate and I/O Performance (SSD 기반 스토리지 시스템에서 중복률과 입출력 성능 향상을 위한 데이터 중복제거 및 재활용 기법)

  • Kim, Ju-Kyeong;Lee, Seung-Kyu;Kim, Deok-Hwan
    • Journal of the Institute of Electronics and Information Engineers
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    • v.49 no.12
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    • pp.149-155
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    • 2012
  • SSD is a storage device of having high-performance controller and cache buffer and consists of many NAND flash memories. Because NAND flash memory does not support in-place update, valid pages are invalidated when update and erase operations are issued in file system and then invalid pages are completely deleted via garbage collection. However, garbage collection performs many erase operations of long latency and then it reduces I/O performance and increases wear leveling in SSD. In this paper, we propose a new method of de-duplicating valid data and recycling invalid data. The method de-duplicates valid data and then recycles invalid data so that it improves de-duplication ratio. Due to reducing number of writes and garbage collection, the method could increase I/O performance and decrease wear leveling in SSD. Experimental result shows that it can reduce maximum 20% number of garbage collections and 9% I/O latency than those of general case.

The Statement in KYE Collection by Designer Kathleen Kye (디자이너 계한희의 카이(KYE) 컬렉션에 나타난 스테이트먼트)

  • Lee, Dah-Yeh;Park, Juhee
    • Journal of the Korean Society of Costume
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    • v.67 no.4
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    • pp.1-20
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    • 2017
  • Centered on Kathleen Kye, a designer who conveyed messages on social issues through her dresses, and established her own identity, this study aimed to examine what theme, method of expression and inner meaning her 'statement' had, as a way of expressing a critical awareness. After reviewing discussions by Duggan G. G. as well as dictionary definitions, statement fashion design can be defined as a work or fashion shows containing a message of a designer who is free from a particular trend or consumerism in various social issues. Statement fashion designers give opposing messages on social issues through their fashion collections. As a result, research shows that contemporary statement fashion designers are expressing themes of a fashion systems, fetishism, body image, collision, environment, as well as the socially disadvantaged by appropriation, reuse, slogan, metaphorical pattern and performance. Satire, awakening, challenge and support can be derived from the inner meaning of contemporary statement fashion design. In terms of the theme, method of expression and inner meaning, this study showed that KYE collection of the designer Kathleen Kye expresses critical awareness on the modern society. Research findings reveal that KYE collection include the following themes: long-term youth unemployment, conflict collision in war or on the Internet, fetishism by youth in a get-rich-quick fever, environmental issue causing destruction of an ecosystem by decrease in bee population, school violence and the socially disadvantaged related to alienated immigrants. Also, as a method of expression themes, such as metaphorical patterns, were used. The patterns used images including a skeleton, gun, heart, rope, plaster, homeless box, bee, honeycomb, chain and a slot machine. On the other hand, the inner meaning of statement in KYE collection showed satire on social issues, awakening on social issues unrecognized by the masses, and support for the socially disadvantaged.

Development of a High-Resolution Multi-Locus Microsatellite Typing Method for Colletotrichum gloeosporioides

  • Mehta, Nikita;Hagen, Ferry;Aamir, Sadaf;Singh, Sanjay K.;Baghela, Abhishek
    • Mycobiology
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    • v.45 no.4
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    • pp.401-408
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    • 2017
  • Colletotrichum gloeosporioides is an economically important fungal pathogen causing substantial yield losses indifferent host plants. To understand the genetic diversity and molecular epidemiology of this fungus, we have developed a novel, high-resolution multi-locus microsatellite typing (MLMT) method. Bioinformatic analysis of C. gloeosporioides unannotated genome sequence yielded eight potential microsatellite loci, of which five, CG1 $(GT)_n$, CG2 $(GT1)_n$, CG3 $(TC)_n$, CG4 $(CT)_n$, and CG5 $(CT1)_n$ were selected for further study based on their universal amplification potential, reproducibility, and repeat number polymorphism. The selected microsatellites were used to analyze 31 strains of C. gloeosporioides isolated from 20 different host plants from India. All microsatellite loci were found to be polymorphic, and the approximate fragment sizes of microsatellite loci CG1, CG2, CG3, CG4, and CG5 were in ranges of 213-241, 197-227, 231-265, 209-275, and 132-188, respectively. Among the 31 isolates, 55 different genotypes were identified. The Simpson's index of diversity (D) values for the individual locus ranged from 0.79 to 0.92, with the D value of all combined five microsatellite loci being 0.99. Microsatellite data analysis revealed that isolates from Ocimum sanctum, Capsicum annuum (chili pepper), and Mangifera indica (mango) formed distinct clusters, therefore exhibited some level of correlation between certain genotypes and host. The developed MLMT method would be a powerful tool for studying the genetic diversity and any possible genotype-host correlation in C. gloeosporioides.

Indoor Path Recognition Based on Wi-Fi Fingerprints

  • Donggyu Lee;Jaehyun Yoo
    • Journal of Positioning, Navigation, and Timing
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    • v.12 no.2
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    • pp.91-100
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    • 2023
  • The existing indoor localization method using Wi-Fi fingerprinting has a high collection cost and relatively low accuracy, thus requiring integrated correction of convergence with other technologies. This paper proposes a new method that significantly reduces collection costs compared to existing methods using Wi-Fi fingerprinting. Furthermore, it does not require labeling of data at collection and can estimate pedestrian travel paths even in large indoor spaces. The proposed pedestrian movement path estimation process is as follows. Data collection is accomplished by setting up a feature area near an indoor space intersection, moving through the set feature areas, and then collecting data without labels. The collected data are processed using Kernel Linear Discriminant Analysis (KLDA) and the valley point of the Euclidean distance value between two data is obtained within the feature space of the data. We build learning data by labeling data corresponding to valley points and some nearby data by feature area numbers, and labeling data between valley points and other valley points as path data between each corresponding feature area. Finally, for testing, data are collected randomly through indoor space, KLDA is applied as previous data to build test data, the K-Nearest Neighbor (K-NN) algorithm is applied, and the path of movement of test data is estimated by applying a correction algorithm to estimate only routes that can be reached from the most recently estimated location. The estimation results verified the accuracy by comparing the true paths in indoor space with those estimated by the proposed method and achieved approximately 90.8% and 81.4% accuracy in two experimental spaces, respectively.

Study on the Filter Collection Methods of Ammonia and Ammonium Salts in the Atomosphere (대기중의 암모니아 및 암모늄염의 필터포집법에 관한 연구)

  • Hui Kang Kim;橋本芳一;Yong Keun Lee
    • Journal of the Korean Chemical Society
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    • v.26 no.1
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    • pp.36-42
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    • 1982
  • Filters were evaluated to use in the collection of ammonia and ammonium salts in the atmosphere. Ammonia from standard gas generator was collected on a glass fiber filter impregnated with a mixture of 3% boric acid and 25% glycerin. The collection efficiency by the impregnated filter was 96.4${\pm}$2.15% in pH control method and 97.4${\pm}$1.06% in the atmosphere for five measurements, respectively. Adsorption and desorption of gaseous ammonia were compared using three commercially available filters; glass fiber, quartz fiber and polycarbonate filters. Both glass and quartz fiber filters indicated some loss of ammonium salts and adsorption of ammonia, respectively. However, polycarbonate filter was found to be satisfactory for the collection of ammonium salts in the atmosphere. The minimum measurable concentration of ammonia was 0.83ppb (ca. 0.63${\mu}g$/$m^3$) by spectrophotometry of the indophenol method for the sample collected by 47mm${\phi}$ filter(20l/min, 60min). The sensitivity of the present method is about 20 folds higher than that of conventional method of bubbler collection followed by spectrophotometry, so that this method makes it possible to measure thevariation of ammoniacal concentrations in the atmosphere for a short time period of about 60 min.

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Complexity Control Method of Chaos Dynamics in Recurrent Neural Networks

  • Sakai, Masao;Homma, Noriyasu;Abe, Kenichi
    • Transactions on Control, Automation and Systems Engineering
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    • v.4 no.2
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    • pp.124-129
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    • 2002
  • This paper demonstrates that the largest Lyapunov exponent λ of recurrent neural networks can be controlled efficiently by a stochastic gradient method. An essential core of the proposed method is a novel stochastic approximate formulation of the Lyapunov exponent λ as a function of the network parameters such as connection weights and thresholds of neural activation functions. By a gradient method, a direct calculation to minimize a square error (λ - λ$\^$obj/)$^2$, where λ$\^$obj/ is a desired exponent value, needs gradients collection through time which are given by a recursive calculation from past to present values. The collection is computationally expensive and causes unstable control of the exponent for networks with chaotic dynamics because of chaotic instability. The stochastic formulation derived in this paper gives us an approximation of the gradients collection in a fashion without the recursive calculation. This approximation can realize not only a faster calculation of the gradient, but also stable control for chaotic dynamics. Due to the non-recursive calculation. without respect to the time evolutions, the running times of this approximation grow only about as N$^2$ compared to as N$\^$5/T that is of the direct calculation method. It is also shown by simulation studies that the approximation is a robust formulation for the network size and that proposed method can control the chaos dynamics in recurrent neural networks efficiently.