• Interdisciplinary Bio Central
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• v.2 no.4
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• pp.12.1-12.7
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• 2010

The National Bio Resource Project-Rat (NBRP-Rat) comprises the largest bank of laboratory rat (Rattus norvegicus) strains in the world. Its main focus is to develop infrastructure that will facilitate the systematic collection, preservation, and provision of rat strains. To breed effectively more than 180 rat strains in living stock, we establish the genetic control system in which a systematic set of genetic diagnoses and genetic monitoring are included. Genetic monitoring is performed by using 20 polymorphic markers. Monitoring is carried out when a living animal stock is re-established by using cryopreserved embryos or sperm or when a rat strain is first introduced to the NBRP-Rat by a depositor. Additional monitoring is then carried out on each strain every two years. Genetic diagnosis is performed largely by employing the Amp-FTA method. Protocols which detail how to perform a genetic diagnosis of 11 transgenes and 24 mutations have been made. Among the mutations, nine can be detected by simple gel electrophoresis of the PCR products, 11 by restriction enzyme treatment of the PCR products, and four by direct PCR product sequencing. Using this genetic control system, the NBRP-Rat can guarantee the genetic quality of its rat strains.

• Conservation Science in Museum
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• v.20
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• pp.61-76
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• 2018

The National Museum of Korea conducted conservation treatmenton the armor in its collection for the purposes of public display and appropriate preservation. This was preceded by a literature study on the types and features of the armor in order to collect basic data for secure and accurate conservation treatment. The literature study found that during the Joseon dynasty, armor was named in reflection of precise details including the color, material, status of the wearer, and even the certain parts of a suit of armor. In general, the name of armor includes the details in the order of color, underlying textile, and scale material (e.g., iron, leather). The former part of the name presents the features of the garment and the latter part refers to the material of the scales or the status of the wearer. The study also found that main materials used in armor include textiles, leather, and metal, and armor can be classified by the materials of the scales-e.g., metal armor (鐵甲), leather armor (皮甲), paper armor (紙甲), paper-and-fabric armor (淹心甲), silk armor (緞甲). Joseon-period armor can also be classified into four types according to its structure and the method of wearing, and overcoat(袍)-style armor was the most widely used in the period following the Japanese Invasion of Joseon (1592-1598) through the late nineteenth~early twentieth century. Overcoat-style armor was commonly worn by infantry, and the four examples of armor with leather scales at the National Museum of Korea belong to this category.

• Reproductive and Developmental Biology
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• v.35 no.4
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• pp.479-483
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• 2011

The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, $Seminark^{Pro}$ (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at $17^{\circ}C$ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/$Dead^{(R)}$ stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at $17^{\circ}C$) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90~6.86) and T4 (6.86~6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0~7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 ($20{\times}10^6$ sperm cells/ml : $5{\times}10^5$ cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.

• Journal of Embryo Transfer
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• v.25 no.4
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• pp.263-266
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• 2010

Prediction of semen's fertilizing ability used in artificial insemination (AI) is one of very important factors on pig reproductive performance. In vitro fertilization (IVF) has been used for indirect evaluation of sperm's fertilizing ability and it has been showed as highly correlated index. In swine industry, increasing interest in preservation of boar semen raises questions on the sperm motility from semen used in commercial AI centers. Mitochondria in sperm mid-piece generate the energy to support motility and could be an explanation of impaired fertility. Objective of this study was to suggest usable sperm motility to farms in measuring the effect of sperm motility and sperm abnormality on in vitro production of embryo in which sperm's fertilizing ability can be determined indirectly. Semen samples were provided from local AI center and used within 3 days after collection. Semen samples were divided by 4 different motile groups (>70%; 61~70%; 51~60%; <50%) using CASA (computer-assisted sperm analysis) on the days of IVF. Developmental rate to the blastocyst stage from over 61% motile sperm group showed significantly higher rate than below 60% motile sperm group ($16.5{\pm}0.7{\sim}18.4{\pm}0.8%$ vs $6.3{\pm}0.8{\sim}11.5{\pm}0.7%$, p<0.05). In experiment to determine the relationship between sperm motility and viability and abnormality, over 61% motile sperm groups showed significantly higher viability rate compared to below 60% motile sperm groups ($84.8{\pm}4.0{\sim}88.1{\pm}4.0%$ vs $69.1{\pm}4.0{\sim}74.2{\pm}4.0%$, p<0.05). On the other hand, morphological sperm abnormality showed significantly higher in over 70% motile sperm group ($10.2{\pm}2.2$ vs $16.0{\pm}2.2{\sim}21.0{\pm}2.2%$, p<0.05). In experiment to find the correlation between sperm motility of 4 different motile groups and amount of mitochondria, lower motility group also showed lower level of mitochondria (p<0.05). The mitochondria parameter used in this study showed another possibility to differentiate the sperm motility. Taken together, because below 60% motile semen used in AI reduce the fertility, AI centers should provide the over 60% motile sperm to the farms at the time of AI.

• The Journal of Korean Medical History
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• v.17 no.1
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• pp.31-55
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• 2004

For the sake of the Restoration(to the original state) of "Sanbeon-bang(刪繁方)", in this study observed carefully the bibliographical results of "Oedaebiyo-bang", "Cheongeumyo-bang", "Cheongeumik-bang" and "Uisim-bang", which directly quoted "Sanbeon-bang". "Sanbeon-bang" is mostly remained in "Oedaebiyo-bang", and the other provisions of "Sanbeon-bang" mostly lie scattered in "Cheongeumyo-bang" and "Cheongeumik-bang", established 100years before "Oedaebiyo-bang" and "Uisim-bang", established 200years after "Oedaebiyo-bang". And there are few other documents which quoted the "Sanbeon-bang". "Cheongeumyo-bang", a synthetic medical work was compiled in 652, and which refered large quantity of medical documents before Dang-dynasty(唐代) by Son Sa-mak(孫思邈). And he linked the technical opinion of himself. The comments of medicine, as well as the prescriptions are refleting the outcome before Dang-dynasty systematically. For the most part of the medical arguments in "Sanbeon-bang" lie scattered in "Cheongeumyo-bang". "Cheongeumik-bang", also a synthetic medical work which is for the sake of supplement of "Cheongeumyo-bang" was complied in 682 by Son Sa-mak. As well as "CheongeumYo-bang" it doesn't make clear the sources of quotation from "Sanbeon-bang". So it used as the data for confirmation. "Oedaebiyo-bang" was compiled in 752 of Dang-Dynasty by Wang Do(王燾) the a governor-general of Eop-gun. Wang Do was descended from a noble family, and usually visited the palace and dealed with large number of books in Hongmunguan(弘文館), the national library. He programmed the book on the base of wide scopic collection of medical documents. And he took charge of gverment post at the same time as Wang Bing(王氷) who compiled "Chaju-Hwangje-Naegyeong-Somun(次注黃帝內經素問)" in 762. So we can guess they might have seen the same documents and holded the medical knowledge in common. The 40 volume "Oedaebiyo-bang" was a great medical complete book in those days. In particular, it became the model of medical complete books of after ages. The description of the book is mostly the qutation of medical documents of before ages. The character is recording of quotation documents and the order of volume. On the base of the recording, we can reconstruct the table of contents of Sanbeon-bang. By way of the contens, we can understand the purpose of "Sanbeon-bang". Besides, I can see a lot of qouotations of "Sanbeon-bang" in "Cheongeum-bang" and "Uisim-bang" are repeatedly quotated in "Oedaebiyo-bang". For that reason, I observed the bibliographical results of "Oedaebiyo-bang" in detail. "Uisim-bang" was compiled in 982 by the Japanese Niwayasyori(丹波綱賴). It followed the example of the structure of "Oedaebiyo-bang", refered to the contents of "Cheongeumyo-bang", collected chinese medical classic books spreaded in Japan. It contains medical documents of before Su-Dang(隋唐)-era, indicated the source of quotation. The importance of the book is summarization and preservation of large quantity of chinese medicine before 10th century. In this study, a lot of the quotation in "Uisim-bang" from "Sanbeon-bang" were not in "Oedaebiyo-bang".

• Korean Journal of Health Education and Promotion
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• v.19 no.2
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• pp.115-125
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• 2002

This study is a investigation research to examine total 123 children's thought, perception and coping method about measles, with 62 children who had experienced the measles and 61 good health children in the school, which had spread to a primary school in kyoungsang-bukdo province. The data collection was done from December 6 to December 8. The analysis of data was SPSS(10.0) for descriptive statistics. The data were collected from total 123 children, 62 of them who had diagnosed measles on October, 2000, when the measles had ended, were used a purposive sampling, the others who had a good health were used a convenience sampling. From three to six grade students made out directly the questionaire in the way of self-questionaire, and from one to two grade students, made out answering the question which the researcher had read the items. The researchers call back the students to complement the contents about the items inadequate answers, so they raised the reliability. The results were as follows. 1. The results of observe carefully the measles infection degree by a sex, the boys(27.4%) were sick severely compared with girls(9.7%)were sick weakly compared with the boys(27.4%)(p〈0.01). 2. The 62.9 percents of infected children by measles thought that they were infected at school, regarded the doctors as a first judge, and had caught the measles because of their fault. The results of observe carefully, 33.8 percents children of infected by measles had have a secondary vaccination. To the question whether they go to institute or not while suffering from the measles, 35.5% children didn't go there since they had heard the fact they infected the measles. 3. To a question of asking if close mend caught the measles, what will they do, 65.1% children of caught the measles answer ‘I can't meet my friend'(p〈0.001). It was significant that infected children have more interests than didn't infected children. The 22% children of didn't catch the measles take great interested in health compare with the 12.2% children of infected the measles(p〈0.05). To the question if they listen the preservation of health broadcasting, 63.4% children answer ‘I listen well’. It was significant between the infected group and didn't infect group(p〈0.05). To the question if they delivery to the written information at home, it was significant between the infected group and didn't infect group(p〈0.05). 4. The infected group of 69.1% children think the health is most important in their life school than any other they like study.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.1
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• pp.78-88
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• 2018

The purpose of this study is to establish a utilization plan according to the cultural value of Placenta Chamber of King Sejong's Princes. We used SNS to analyze various public perceptions and opinions, collected data and analyzed it. The collection period is from June 01, 2007 to June 30, 2017 (for about 10 years), We gathered data from blogs, cafes, and Knowledge IN that contain keywords related to 'Placenta Chamber', 'Placenta Chamber of Seongju', 'Placenta Chamber of King Sejong's Princes'. and Analyzed using the text mining method of the big date program. Based on the main results of the big data analysis, Placenta Chamber's method of utilization was derived. As a result, major keywords such as King Sejong Great, Prince, Sungju, Feng Shui, culture, preservation, blessing etc were derived. The association of 'world', 'heritage', 'cultural heritage' is high, and the connection of 'Placenta Chamber', 'Gyeongsangbuk-do', 'cultural property' is high, and it was able to confirm the value of Placenta Chamber as a world cultural heritage. and It is necessary to induce visitors to feel stimulation or change of surroundings through facility refurbishment and environmental improvement around Placenta Chamber.

• Hwankyungkyoyuk
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• v.20 no.2
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• pp.93-107
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• 2007

Livestock agriculture plays an essential role Hongsung's economy. Unfortunately, if manure is manage properly it can contaminate water and pollute the air. The purposes of this study were to provide awareness of environmental impact and to design educational programs with sustainable agriculture. I used the specific steps of processes. First, collecting informations through survey on environmental awareness of livestock farmers in Hongsung-gun and recognizing the tendencies. Second, analyzing on educational status of livestock farmers. Third, finding objectives of environmental educational programs. At the end of processes, establishing programs which concerning on environmental education. I used two research methods to gather and analyze the data for developing an environmental education program. The first one is a general survey among 50 livestock farmers from Hongseong-gun, Chungcheongnam-do. The second one is the in-depth interviews with specialists in environmental education and government officials from the central government - the Ministry of Agriculture and Forestry - and the local government - Hongsung-gun who are in charge of livestock farming. I found the following results from this research. First, most livestock farmers replied with 'highly likely' to the five items on the recognition of the necessity of environment preservation, whereas only 38.7% answered with 'likely' to the item on the environmental pollution caused by livestock farming. This shows the tendency that they have less recognition of the reality than necessary. Second, most of the contents among 5 areas(knowledge, recognition, function, attitude, and participation) are the knowledge areas. That results are analyzed training materials for livestock farmers made by Hongsung-gun in 2004 and in 2005 by using goal categories suggested in the Tbilisi Resolution. Third, after analyzing the survey and in-depth interviews, I found that the need for education to recognize the environmental pollution, environmental protection and recycling of the livestock waste. We can summarize conclusions from this research. First, we need on educational program improve the livestock farmers' recognition of importance in environmental protection. Second, it is necessary to develop educational program with the viewpoint that livestock waste is not one of the culprits of the environmental pollution, but a recyclable resource. Third, contents of the environmental education program, should include all the educational elements, namely, knowledge, recognition, attitude, function and participation. The education for livestock farmers requires a research on the environmental education program that can be used with crop farmers who can use the resources recycled from the livestock waste from the viewpoint of recycling resources. This research is focused on the collection and analysis of the basic data for developing an environmental education program and the composition of the program. A further research on the implementation and assessment of this program is required.

• Journal of Korean Society of Archives and Records Management
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• v.21 no.3
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• pp.163-186
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• 2021

The purpose of this study is to analyze research trends on overseas business archives, compare them with domestic and related prior studies, and present the direction of the development of domestic business archives research. For the research analysis, bibliography from 493 papers was collected using four academic databases: Web of Science, Scopus, LISA (ProQuest), LISS (EBSCOhost). Following data collection, data analysis was conducted and focused on the publication status of related papers, major journals, research forms, and researcher information. In addition, unique words were extracted through the data processing of English abstracts, and the frequency of the key words was identified. Through topic modeling, this study extracted seven topics from the entire literature group, analyzed the publication trends of the papers corresponding to each topic, and examined the topics and representative papers of that topic. The names for these topics were assigned as "access and usability of business records'," "information/evidence value of business records," "importance and operational strategy of business archives," "record management as a business heritage," "management of business records in digital environments," "preservation and disposal of business records," and "historical value of business records." Based on the prior research and analysis results, this study proposed implications for the development and expansion of future research on the domestic business archives.

• The Journal of the Korea Contents Association
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• v.21 no.8
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• pp.49-59
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• 2021

Since ancient times, legends and tales have been handed down with a spirituality, shamanistic meaning, and imagination. Among many tales about people and animal footprints that are handed down in various parts of Korea, Changnyeong's 'Munhojang Footprint' is the first case in which the physical evidence(footprints) that the main character has left was identified as a dinosaur footprint. In this study, we performed a scientific analysis based on the basic data collection, distribution pattern of 'Munhojang Footprint', three-dimensional digital recording and visualization, as well as case analysis and humanitic review of footprints in tales and legends. The Munhojang Footprints has long been known as human footprints left in the natural rock due to its shape and preservation status. A new analysis that the Munhojang footprints (composed of 13 footprints) are dinosaur tracks shows social perceptions of the ancient people, characterized by the fear of supernatural beings and the limits of scientific interpretation. Through this scientific and humanistic exploration of Munhojang Footprint that are passed down from generation to generation as legends, pray for peace and well-being of the village through rituals and rituals every year, and have been preserved and managed as practical evidence, it is expected that traditional culture and natural heritage will be linked and mutual value will be enhanced.