• Journal of Food Hygiene and Safety
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• v.31 no.3
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• pp.222-225
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• 2016

In this study, the performance of five selective media for coliform bacteria was evaluated. In total, 83 coliform isolates from ready-to-eat food and 21 reference strains were inoculated in five agar media : Chromocult coliform agar (Merck Millipore), HiCrome coliform agar (Sigma), CHROMagar ECC chromogenic media, Brilliance E. coli/coliform selective agar (OXOID), and endo agar (Merck Millipore). Coliform isolates and reference strains were inoculated on the selective media to test media sensitivity and specificity. The tested media showed the following sensitivities for the isolated strains: Chromocult coliforms agar and HiCrome coliform agar, 94%; Brilliance E. coli/coliform selective agar, 93%; CHROMagar ECC chromogenic media, 92%; and endo agar, 74%. In addition, all media showed 100% specificity, except for endo agar (71%). Moreover Chromocult coliform agar and HICrome coliform agar showed high levels recovery. Taken together, these results identified Chromocult coliform agar and HICrome coliform agar as an effective selective medium for coliforms with higher sensitivity and specificity compared to other media tested in this study.

• Journal of Korean Society on Water Environment
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• v.22 no.1
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• pp.166-171
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• 2006

The objectives of the present paper were to investigate the concentration of total coliform in wastewater effluents and the effect of water chemical and physical characters in it. The most correlated parameter with total coliform was COD. It means that the wastewater treatment efficient effects on total coliform concentration. And we developed predictive model for the total coliform concentration. The estimated parameters for model were COD, temperature, nitrite, chloride, Mn and regression model equation was determined; log (Total Coli.) = 1.861+0.065[COD]+0.038[temperature]-0.0004[$Cl^-$]+3.697[Mn]-0.32 [$NO_2-N$] The developed model provided very strong correlation ($R^2:0.82$) between total coliform and regression equation. The parameters having high sensitivity were COD and temperature. So the study indicated that if the temperature and COD of wastewater effluent were known, we would estimate the concentration of total coliform and decide the most effective usage of chlorine.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.2
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• pp.41-51
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• 1974

A study of the sanitary quality of Pacific oysters (Crassostrea gigas) and growing waters associated with raft culture in Burley Lagoon, Washington was conducted. The study was sponsored by the Agency for International Development of the U.S. Department of State. The results obtained in this study are as follows: The average values of temperature, salinity and turbidity in the water showed that the values of bottom sample were slightly higher than top samples. The difference was about $0.3^{\circ}C$ for temperature, $0.5\%_{\circ}$ for salinity and 0.1 JTU for turbidity. The changes of temperature and salinity by tide generally followed the tide cycle pattern. Sanitary indicator microorganism concentrations in top water were generally higher than those in bottom water. In general, the levels of mean coliform and fecal coliform MPN's varied inversely with tide level indicating that the sources of these groups of microorganisms are the fresh water streams flowing into the estuary. The $35^{\circ}C$ plate counts were more stable at different tide levels. Mean values of coliform and fecal coliform MPN's in oysters demonstrated that levels in top oysters were generally higher than those in bottom oysters. However, mean values of $35^{\circ}C$ Plate count in oysters did not show this pattern. The mean levels of both coliform and fecal coliform MPN's in oysters also correlated inversely with tidal level. The accumulation ratios of oysters obtained during the study period ranged from 8.6 to 19.7 for mean coliform MPN and 16.9 to 44.3 for fecal coliform MPN. According to the results obtained from present study, one suggestion could be of considerable importance for the sanitary operation in hanging culture of pacific oysters. The results indicate that harvest of the oysters at high tide would result in lower levels of indicator organisms in the shellfish.

• Journal of Environmental Health Sciences
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• v.32 no.3
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• pp.215-221
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• 2006

This study investigated the inactivation of the total coliform, an indicator organism in chlorine dioxide, in order to assess the optimal disinfection procedure for drinking water treatment and distribution systems. This research focus on a number of factors, including the dosage of disinfectant, contact time, pH, temperature and DOC. Water samples were taken from the outlet of a settling basin at a conventional surface water treatment system. As the pH increased in the range of pH 6-9, the bactericidal effects of disinfectants decreased. Changes in levels of pH did not significantly after the disinfection effect of chlorine dioxide for total coliform. With an increase in temperature, there was a subsequent increase in the bactericidal effects of disinfectants. Thus, it is evident that a decrease in temperature will higher the CT values required to inactivate coliform for during the winter. DOC addition can also reduce total coliform inactivation. DOC is the most significant variable in total coliform inactivation with chlorine dioxide.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.1
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• pp.77-81
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• 1998

A bacteriological study of sea water in Deukryang Bay was conducted to evaluate sanitary conditions of the bay and compliance of waters with the recommended bacteriological criteria for the designated area of shellfish cultivation. Sea water samples were collected at the established sampling stations (fig. 1) from May 1995 to November 1996. During the study period, coliform group, fecal coliform, classification of coliform group with IMViC reactions and pathogenic vibrios were analyzed. Coliform group and feral coliform MPN's were ranged from $<\;3.0\~4600/100m{\ell}\;and\;<\;30\~1,100/100m{\ell}$, respectively. The bacteriological criteria of sea water in shellfish growing area should be less than 70 per l00ml of sea water for median value of coliform MPN, and below $10\%$ of the samples which contain over than 230 for coliform MPN or over than 43 for fecal coliform MPN, Most of the waters from 26 sampling stations were complied water coliform criteria recommended for designated shellfish growing area. Then, the ratios of the samples with move than 230/10ml of coliform group MPN and more than 43/100ml of fecal coliform MPN were $7.4\%$ and $8.5\%$, respectively. The bacterial density of the sea water was deeply affected by rainfall amount. For example, coliform bacterial counts of sea watery after 48 hours from 93 mm rainfall were $6\~7$ times higher than those of without rainfall. During the study period, infectious bacteria such as Vibrio cholerae, Salmonella sp. an d Shigella sp. were not detected from the samples, but detection ratios of Vibrio parahaemolyticus and Vibrio vulnificus were $15\~20\%$ in summer months.

• Journal of Environmental Health Sciences
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• v.28 no.2
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• pp.193-202
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• 2002

The polymerase chain reaction(PCR) of target lacZ and uidA genes were used to detect total coliform and Escherichia coli for determining water quality, respectively. Of 109 spring waters, coliform were detected from 38 spring waters by lacZ PCR method but 21 spring waters by culture method accepted by the Ministry of Environment for water quality monitoring. The lacz PCR method gave the results statistically equivalent to those of the culture method(kappa=0.62, McNemar=17.00). The uidA PCR method gave the same results to those of the culture method. The sensitivity and specificity of coliform and E. coli by PCR method were 100% and 80.7%, respectively. Therefore, PCR can be used for the rapid identification of Escherichia coli and coliform in potable water using uidA and lacZ.

• Journal of Environmental Health Sciences
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• v.1 no.1
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• pp.28-31
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• 1974

A study was conducted to find out the biological contamination of wells in Chon Ho Dong areas, Seoul, Korea from May 4, 1971 through May 18 1971. Coliform groups were detected by membrane filter method and general sanitary conditions were checked. The results obtained were summarized as follows: 1) 61 wells (81.3%) out of 75 wells showed the evidence of coliform group. In urban areas it is recommended to use disinfectants for wells pretty frequently. 2) The wells in native villages were less contaminated than those in new villages established by poor people from slum areas of Seoul. 9 out of 15 wells at Song Pa Dong, which is However, at Bang E Dong's new village, 19 out of 20 wells were contaminatd by coliform groups. 3) Coliform groups were positive at 57 out of 61 open wells, while only 4 out of 14 pumping wells showed the evidence of coliform groups. 4) 38 out of 40 poorly drained wells were contaminated, however, 13 out of 25 well drained wells were coliform organisms positive.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.6 no.3_4
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• pp.92-96
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• 1973

This examination was carried out to evaluate the sanitary quality of slices of raw fish being served in the restaurants. Twenty five kinds of slices of raw fish collected from various restaurants in Busan Korea were examined during the period from March to May in 1971. As the evaluation factors of sanitary quality, the contents of sanitary indicative bacteria such as coliform group, fecal coliform, feral streptococci and enterococci and plate counts were determined. The results obtained are as in below: 1. The numbers of fecal streptococci and enterococci MPN were much greater than those of coliform group and fecal coliform. 2. The median value of coliform group MPN was 3,300 per 100 grams of the sample examined and those of enterococci was 5,400. The median value of plate counts was $1.8\times10^5$ per gram. 3. Fifty-two percent of the samples examined were exceeded fecal coliform MPN 930 per 100 grams. 4. As a sanitary indicative bacteria fecal coliform MPN was more reasonable than enterococci 5. The grade of restaurants was not correlated with the bacterial quality of the foods served. 6. No correlation between the numbers of sanitary indicative bacteria ana plate counts was observed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.615-625
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• 2023

This study evaluated the bacteriological safety of seawater and abalone produced in Uisin-myeon, Jindo-gun from September 2020 to December 2022 and calculated the effect of inland pollution sources on seawater and abalone. We surveyed 33 stations for seawater, 4 stations for abalone, and 7 stations for discharge water of major inland pollutants. We analyzed the coliform group, fecal coliform and Escherichia coli (E. coli) as bacteria indicators. In 924 seawater samples, the geometric mean of the coliform group and fecal coliform were <1.8-5.6 MPN/100 mL and <1.8-2.7 MPN/100 mL, respectively. The estimated 90^th percentile range for the coliform group and fecal coliform were <1.8-42 MPN/100 mL and <1.8-8.4 MPN/100 mL, respectively. The fecal coliform and E. coli levels for 107 abalone were <18-460 MPN/100 g and <18-78 MPN/100 g respectively. Moreover, the analyzed standard plate count was <30-7,700 CFU/g. The fecal coliform level and diffusion area in 7 inland pollutants were <1.8-3,300,000 MPN/100 mL and 2-2,500 m, respectively. The bacteriological safety of seawater and abalone in Uisin-myeon, Jindo-gun, was demonstrated to be a designated area according to Korean standards, a conditionally approved area per US standards, and Class A according to EU standards.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.1
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• pp.11-16
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• 2012

In coastal areas that are affected by continuous, seasonal or occasional pollution sources, bivalves accumulate biological contaminants such as pathogenic bacteria. We investigated the effect of natural seawater relaying and electrolyzed seawater depuration on the bacteriological quality of artificially contaminated oysters Crassostrea gigas and short-necked clams Ruditapes philippinarum to suggest an alternative method of shellfish sanitation control.When artificially contaminated oysters and short-necked clams (fecal coliform level 1,700 MPN/100 g) were relayed into a sea area of safe bacteriological water quality, the fecal coliform level dropped to below 110 MPN/100 g after 1 day. The bacteriological quality of oysters and short-necked clams that are contaminated at a fecal coliform level of 1,700 MPN/100 g could be improved, and become appropriate for raw consumption by a single day relay under proper environmental conditions. When artificially contaminated oysters (fecal coliform level 330 MPN/100 g) were depurated with electrolyzed seawater, 94% of fecal coliform was eliminated after 12 h and fecal coliform was undetectable after 24 h. After 24 h depuration with electrolyzed seawater, the fecal coliform level of short-necked clams with initial fecal coliform of 2,400 MPN/100 g was below 20 MPN/100 g. However, the fecal coliform level of short-necked clams with initial fecal coliform of 17,000 MPN/100 g was relatively high, at 790 MPN/100 g, even after 24 h of depuration with electrolyzed seawater, because of the repeated cycle of excretion and accumulation of fecal coliform in shellfish tissue under the closed depuration environment. Such natural seawater relaying and electrolyzed seawater depuration can be restrictively applied to improve or secure the bacteriological quality of oysters and short-necked clams in accordance with safety levels for bivalves for raw consumption.