Browse > Article
http://dx.doi.org/10.13103/JFHS.2016.31.3.222

Comparative Evaluation of Selective Chromogenic Media for Coliforms Bacteria Isolated from Food  

Lee, Da-Yeon (Food Analysis Center, Korea Food Research Institute)
Kim, Hee-Eon (Food Analysis Center, Korea Food Research Institute)
Lee, Jin-Sung (Dept. of Biological Sciences, Kyonggi University)
Kim, Keun-Sung (Dept. of Food Science and Technology, Chung-Ang University)
Cho, Yong-Sun (Food Analysis Center, Korea Food Research Institute)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.3, 2016 , pp. 222-225 More about this Journal
Abstract
In this study, the performance of five selective media for coliform bacteria was evaluated. In total, 83 coliform isolates from ready-to-eat food and 21 reference strains were inoculated in five agar media : Chromocult coliform agar (Merck Millipore), HiCrome coliform agar (Sigma), CHROMagar ECC chromogenic media, Brilliance E. coli/coliform selective agar (OXOID), and endo agar (Merck Millipore). Coliform isolates and reference strains were inoculated on the selective media to test media sensitivity and specificity. The tested media showed the following sensitivities for the isolated strains: Chromocult coliforms agar and HiCrome coliform agar, 94%; Brilliance E. coli/coliform selective agar, 93%; CHROMagar ECC chromogenic media, 92%; and endo agar, 74%. In addition, all media showed 100% specificity, except for endo agar (71%). Moreover Chromocult coliform agar and HICrome coliform agar showed high levels recovery. Taken together, these results identified Chromocult coliform agar and HICrome coliform agar as an effective selective medium for coliforms with higher sensitivity and specificity compared to other media tested in this study.
Keywords
Selective media; Coliforms; Chromogenic media; Evaluation;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Brenner, K.P., Rankin, C.C., Roybal, Y.R., Stelma, G.N.J., Scarpino, P.V., Dufour, A.P.: New Medium for the Simultaneous Detection of Total Coliforms and Escherichia Coli in Water. Appl. Environ. Microbiol., 59, 3534-3544 (1993).
2 MFDS (Ministry of Food and Drug Safety). Korea Food code (2015).
3 Kim, H.J., Oh, S.W.: Performance Comparison of 5 Selective Media used to Detect Staphylococcus aureus in Foods. Food Sci. Biotechnol., 19, 1097-1101 (2010).   DOI
4 Gonzalez, R.D., Tamagnini, L.M., Olmos, P.D., De Sousa, G.B.: Evaluation of a Chromogenic Medium for Total Coliforms and Escherichia Coli Determination in Ready-to-Eat Foods. Food Microbiol., 20, 601-604 (2003).   DOI
5 Kim, S.R., Lee, S.H., Seo, M.K., Kim, W.I., Park, K.H., Yun, H.J., Yoon, Y.H., Yoo, S.Y., Ryu, K.Y., Yun, J.C.: Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products. J. Fd. Hyg Safety, 27, 169-175 (2012).   DOI
6 Samra, Z., Heifetz, M., Talmor, J., Bain, E., Bahar, J.: Evaluation of use of a New Chromogenic Agar in Detection of Urinary Tract Pathogens. J. Clin. Microbiol., 36, 990-994 (1998).
7 Fricker, E.J., Fricker, C.R.: Use of Two presence/absence Systems for the Detection of E. Coli and Coliforms from Water. Water Res., 30, 2226-2228 (1996).   DOI
8 Manafi, M.: New Developments in Chromogenic and Fluorogenic Culture Media. Int. J. Food Microbiol., 60, 205-218 (2000).   DOI
9 Filius, P.M.G., Van, Netten D., Roovers, P.J.E., Vulto, A.G., Gyssens, I.C., Verbrugh, H.A., Endtz, H.P.: Comparative Evaluation of Three Chromogenic Agars for Detection and Rapid Identification of Aerobic Gram-negative Bacteria in the Normal Intestinal Microflora. Clinical microbiology and infection, 9, 912-918 (2003).   DOI
10 Manafi, M.: Fluorogenic and Chromogenic Enzyme Substrates in Culture Media and Identification Tests. Int. J. Food Microbiol., 31, 45-58 (1996).   DOI
11 Kim, H.J., Koo, M.S., Oh, S.W.: Performance Comparison of 3 Different Isolation Media of Cronobacter Sakazakii. J Korean Soc Food Sci Nutri., 39, 764-768 (2010).   DOI
12 Sartory, D.P., Howard, L.: A Medium Detecting ${\beta}$-glucuronidase for the Simultaneous Membrane Filtration Enumeration of Escherichia Coli and Coliforms from Drinking Water. Lett. Appl. Microbiol., 15, 273-276 (1992).   DOI
13 Uyttendaele, M., Bagamboula, C.F., De Smet, E., Van Wilder, S., Debevere, J.: Evaluation of Culture Media for Enrichment and Isolation of Shigella Sonnei and S. Flexneri. Int. J. Food Microbiol., 70, 255-265 (2001).   DOI