• Journal of Society of Preventive Korean Medicine
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• v.18 no.3
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• pp.81-90
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• 2014

Objective : We performed a clinical study to investigate pattern characteristics in persistent allergic rhinitis depending on Korean Medicine pattern questionnaire items as a pattern identification diagnostic tool. Method : 32 patients with persistent allergic rhinitis were asked to interview with doctor of Korean Medicine and perform the 4 pattern questionnaires(Cold-Heat Pattern, Phlegm Pattern, Yin Deficiency pattern, bloodstasis pattern). Then, we analyzed the response rate of each pattern questionnaires. Results : After diagnosis of Korean Medicine Doctor's pattern identification, 17 individual items have higher response rate, 7 of 17 items have a common tendency in allergic rhinitis. The other 8 of 10 items belong to Lung qi deficiency cold and Lung-spleen qi deficiency group, these have higher tendency of deficiency. In bloodstasis pattern questionnaires, we don't decide the tendency of patients with allergic rhinitis. Conclusion : The result may provide that we don't use Korean Medicine pattern questionnaires as a major tool in the pattern identification of allergic rhinitis. Continuous studies are needed to develop the standardized pattern identification diagnostic tool.

• Korean Journal of Microbiology
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• v.38 no.1
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• pp.45-49
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• 2002

Bradyrhizobium japonicum is a soil bacterium with a unique ability to infect the roots of leguminous plants and establish a nitrogen-fixing symbiosis, which has been used as a microbial manure. In this study, we examined the stress response after pretreatment of cells with cold temperature. When pre-treated with cold temperature ($4^{\circ}C$) for 16 hr, B. japonicum increased the viability in subsequent stress-conditions such as alcohol, $H_2O_2$, heat, and dehydration. For cold adpatation, cultured B. japonicum was exposed to $4^{\circ}C$. Upon subsequent exposure to various conditions, the number of adapted cells pretreated by cold adaptation was 10-1000 fold higher than that of non-adaptated ones. It appeared de novo protein synthesis occurred during adaptation, because a protein synthesis inhibitor, chloramphenicol abolished the increased stress tolerance. By using a degenerate PCR primer set, a csp homolog was amplified from B. japonicum genome and sequenced. The deduced partial amino acid sequence of the putative Csp (Cold shock protein) shares a significant similarity with known Csp proteins of other bacteria.

• Journal of Physiology & Pathology in Korean Medicine
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• v.35 no.6
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• pp.274-279
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• 2021

This study was conducted to find objective diagnostic indicators for the Eight Principle Pattern Identification using a pulse wave analyzer. Typology Complexion Pulse and Symptom data from 500 women over the age of 18 were used. Five experts made a diagnosis of Eight Principle Pattern Identification and divided the subjects into 'Yin-Yang', 'Exterior-Interior', 'Cold-Heat', 'Deficiency-Excess' groups. Their pulse waves were measured in the left and right radial arteries, and it was investigated whether there was a significant difference between groups in the pulse wave parameter values. 'Yin' group showed a significantly lower value in the left radial artery for Ener, Emin, EIX, T4T, T4TT parameters and in the Right for T2, T2T, T5. The Vmag, As and Ad parameters were significantly different between the 'Exterior' and 'Interior' groups. 'Heat' group showed a significantly higher value in the right radial artery for RAI/t parameter. 'Deficiency' group showed a significantly higher value in the right radial artery for W, Angl parameters. Through this study, significant pulse wave parameters were found, and they can be used as objective diagnostic indicators for Eight Principle Pattern Identification.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.602-605
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• 2001

Low-temperature adaptation and protection for environmental stresses were studied in the gram-negative soil bacterium Bradyrhizobium japonicum 61A101c. B. japonicum was more resistant to alcohol, $H_2O_2$, heat and freezing following a pretreatment at $4^{\circ}C$, resulting in approximately 10 to 1,000 folds increased survival compared to mid-exponential-phase cells grown at an optimal temperature at $28^{\circ}C$. This phenomena relate to the cold shock protein expressed when cells are exposed to a downshift in temperature. To confirm the presence of cold shock protein genes in B. japonicum, a PCR strategy was employed using a degenerate primer set, which successfully amplified a putative csp gene fragment. Sequence analysis of the PCR product(200bp) revealed csp-like sequences that were up to 96% identical to csp gene of S. typhimurium.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.958-963
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• 2009

I Alcoholic hepatitis is a serious liver disease that may lead to cirrhosis and carcinoma, and the short-term mortality rate is fairly high in severe patients. This study was conducted to develop the instrument of pattern identification for alcoholic hepatitis. We made the pattern identification questionnaire and symptoms indicator through reviewing traditional oriental medical literatures and got advices from the advisor committee with Delphi technique. The advisor committee on this study was organized by 10 professors of internal medicine of oriental medical colleges nationwide. The questionnaire was composed of questions about 6 pattern identification - dampness, heat, liver, spleen, cold and dryness. We gave importance to each symptoms of 6 pattern identification which had been scored on a 5-point scale. We surveyed two groups: 36 male alcoholic hepatitis patients whose Alcohol Use Disorder Identification Test(AUDIT) scores were over 12 and who drank alcohol over 40 g per day were allocated to the hepatitis group. Forty three men who did not drink alcohol were allocated to the normal group. Alcoholic hepatitis had relativities to dampness, heat among cause of disease and liver, spleen among viscera. There were statistical significances between the hepatitis group and the normal group in dampness, heat, liver questionnaire. As a result of this study we suggest that the questionnaire would be effective instruments of pattern identification for alcoholic hepatitis.

• Korean Journal of Oriental Medicine
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• v.11 no.1
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• pp.109-118
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• 2005

In a field of oriental medicine, it is necessary to collect systemically clinical data and integrate. Input data items was decided, then categorized by the modules through discussion of the institute and hospitals. Items are information of patient, history, aspects of occurrence, pattern identification, prescriptions of herbal medicine, the results of biochemical serum examination, blood cell count, urine analysis, CT, MRI, Chest PA, EKG etc. Factors in oriental medicine are Sasang constitution, Stroke-Pattern-Identification(china), differentiation of cold and heat syndrome. This tool was constructed by using Microsoft-Access 2000 and Microsoft Visual Basic 6.0. Furthermore this web-based system could be applied to multi-center clinical investigation.

• The Journal of Korean Medical History
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• v.34 no.2
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• pp.25-43
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• 2021

Among the ancient ideas of East Asia, there is a myth about the symbol of the Four Gods who commands all directions in the east, south, west, and north, and this is also reflected in the medical field. However, although there is discussion about Qinglong Decoction (青龍湯), Baihu Decoction (白虎湯) and Xuanwu Decoction (玄武湯) in the 『Shanghan Lun (傷寒論)』, there is no content focusing on Zhuniao decoction (朱鳥湯). Considering the consistent perspective of 『Shanghan Lun』, which recognizes the disease-syndrome and seeks prescriptions based on yin and yang thinking, this is very unusual. Therefore, in this study, it was revealed that the concept of 'Sishen Decoction (四神湯)' itself was a concept that emerged when the authors of 『Shanghan Lun』 established the 'eight-principles syndrome differentiation (八綱辨證)' system in their recognition and response to diseases-syndromes. Based on this, I considered the following possibilities: They were able to present Qinglong decoction, Baihu Decoction and Xuanwu decoction, as appropriate prescriptions for 'exterior cold excess syndrome (表寒實證)', 'interior heat deficiency syndrome (裏熱虛證)', and 'interior cold deficiency syndrome (裏熱實證)'. However, it is possible that the name of the prescription 'Zhuniao decoction' was not intentionally used, because it was not possible to provide an appropriate prescription for 'exterior cold excess syndrome (表熱實證)'.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.4
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• pp.201-212
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• 2017

This study draws pattern differentiations of headache disorders on the ground of modern clinical applications and Korean medical literature. Categorization and symptoms of headache disorders are based on International Classification of Headache Disorders 3rd edition(beta version). And clinical papers are searched in China Academic Journals(CAJ) of China National Knowledge Infrastructure(CNKI). In the aspect of eight principle pattern identification, primary headache occurs due to lots of yang qi and has more inner pattern rather than exterior pattern, heat pattern rather than cold pattern, excess pattern rather than deficiency pattern. And primary headache is related with liver in the aspect of visceral pattern identification and blood stasis, wind and phlegm are relevant mechanisms. Migraine without aura is associated with ascendant hyperactivity of liver yang, phlegm turbidity, sunken spleen qi, wind-heat, blood deficiency or yin deficiency. Migraine with aura is mainly related with wind and it's major mechanisms are ascendant hyperactivity of liver yang, liver fire, yin deficiency of liver and kidney, blood deficiency or liver depression and qi stagnation. High repetition rate of tension-type headache can be identified as heat pattern or excess pattern. And trigeminal autonomic cephalalgias can also be accepted as heat pattern or excess pattern when the occurrence frequency is high and is relevant to combined pattern with excess pattern of external contraction and deficiency pattern of internal damage based on facial symptoms by external contraction and nervous and anxious status by liver deficiency. This study can be expected to be Korean medical basis of clinical practice guidelines on headache by proposing pattern identifications corresponding to the western classifications of headache disorders.

• The Journal of Internal Korean Medicine
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• v.39 no.4
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• pp.495-510
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• 2018

Objective: This study was designed to investigate the possibility of quantification of the diagnosis of abdominal coldness (AC) in patients with functional dyspepsia (FD). Methods: Forty-four patients with FD were enrolled in this study. Three Korean medicine doctors each randomly examined all abdomens. Diagnosis of AC was made by consensus of at least two of the doctors. Body temperature (oral by digital oral thermometer) and skin temperature (by digital infrared thermal imaging [DITI]) were measured, followed by administration of the Cold and Heat questionnaire (CHQ) and the Instrument of Pattern Identification for Functional Dyspepsia (IPIFD). Results: Of the 44 patients with FD, 22 were assigned to the AC group and 22 to the non-AC group. The concordance rate of diagnosis among the three doctors was 63.6% (28/44), with a ${\kappa}$ of 0.504, indicating means moderate agreement). Neither the oral nor the skin temperatures showed statistically significant differences between the AC and non-AC groups. However, the CHQ scores and 'Simultaneous Occurrence of Cold and Heat Syndromes pattern' scores of the IPIFD were higher in AC group and showed statistically significant differences (p=0.010 and 0.009). Conclusions: This is the first study conducting quantitative measurements of abdominal coldness in patients with FD. Although oral and skin temperature showed no statistical significance between AC and non-AC groups, the concordance rate of diagnosis of AC among the three Korean Medicine doctors was moderate. The CHQ scores and 'Simultaneous Occurrence of Cold and Heat Syndromes pattern' scores of the IPIFD also suggest that diagnosis of AC is relevant to cold and heat patterns, and these questionnaires could be utilized as supportive data for the diagnosis of AC. Further studies should be conducted for the purpose of quantifying and standardizing abdominal examinations in Korean Medicine.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.6
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• pp.668-673
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• 2014

Cold and Heat pattern identification(CHPI) in traditional East Asian medicine(TEAM) is one of the major indicator to distinguish characteristics of disease and to determine treatment method. Basic parameters to determine CHPI include the pulse rate, respiration rate, and pulse power. Studies to associate physiological responses of human body by cold stress(CS) with CHPI in TEAM were rarely done so far. This study aims to explore the effects of cold stress on pulse signal via a feasibility study for three subjects and investigate some indices which can reflect autonomic nerve reaction(ANR). We measured radial pulse signals and respiration signal of the investigated subjects before the CS, during the CS which continues for 5 minutes, and immediately after the CS, respectively. Finally, we analyzed the pulse rate (P), respiration rate (R), pulse power, pulse depth, and pulse rate per respiration (P/R ratio). As a result, the P/R ratio showed a consistently decreasing tendency through the CS stimulation process, while other parameters behaved more complex and in subject-specific ways. It implies that, among candidate parameters, the P/R ratio is a simple but the most probable parameter that can be used as the ANR indicator. This result is also consistent with the theory in TEAM scripts, in which the P/R ratio is predicted to be a direct indicator for the CHPI. This pilot study shows that P/R ratio can be more appropriately associated with the ANR than heart rate or respiration rate alone. Extensive studies will be necessary to verify or confirm the P/R ratio as an appropriate and well defined parameter for ANR.