• Journal of Applied Biological Chemistry
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• v.58 no.4
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• pp.325-332
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• 2015

Rapeseed (Brassica napus) is now the second largest oilseed crop after soybean. Cold temperature tolerance is an important agronomic trait in winter rapeseed that determines the plant's ability to control below freezing temperatures. To improve cold tolerance of rapeseed plants, an expression vector containing an Barley Ice recrystallization inhibition protein (HvIRIP) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into rapeseed plants. Transgenic expression of HvIRIP was proved by southern- and northern-blot analyses. The level of freezing tolerance of transgenic $T_3$ plants was found to be significantly greater than that of wild-type rapeseed plants by freezing assay. Proline accumulation during cold stress was also highly induced in the transgenic rapeseed plants. The transgenic plants exhibited considerable tolerance against oxidative damage induced by cold stress. Our results indicated that heterologous HvIRIP expression in transgenic rapeseed plants may induce several oxidative-stress responsive genes to protect from cold stress.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.484-492
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• 2019

The aim of the present study was to compare the cold tolerance of seven different types of trees growing in southern Korea to select evergreen broad-leaved trees that can be used as street trees in large land areas experiencing climate change. The trees compared were the thorn tree, Cinnamomum camphora, Camellia japonica, Machilus thunbergii, Dendropanax morbifera, Daphniphyllum macropodum Miq., Quercus glauca Thunb., and Raphiolepis indica. When the trees were subjected to low temperature treatment, their electrolyte elution volume values appeared to increase with the decreases in the treatment temperature. The analysis of the cold tolerance of each type of tree was based on the estimated temperatures in the following order: C. japonica (-11.586℃) > R. indica (-9.348℃) > Q. glauca (-8.719℃) > M. thunbergii (-8.090℃) > D. macropodum (-7.409℃) > D. morbifera (-7.085℃) > C. camphora (-6.995℃). The relative cold tolerance difference found in the seven tree species was more than 5℃, as evaluated previously. In the Lauraceae family, the difference in cold tolerance was more than 2℃, even in the same species. The analysis showed that trees with excellent cold tolerance included Q. glauca Thunb., C. japonica, R. indica, and the thorn tree. This knowledge is required for the evaluation of the possibility of the survival of trees under cold temperature conditions in cities.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2001.10a
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• pp.170-173
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• 2001

The design for cold extrusion dies is very important because the die insert is subjected to very high radial and hoop stresses. The design of cold extrusion dies has many constrained conditions. In this paper, two assumptions were proposed. First assumption was selected by yielding strength dependent on the to hoop stress of each ring in dies. Second assumption is that the maximum inner pressure is determined when yielding occurs in one ring of dies. To obtain the maximum inner pressure the flexible tolerance method was applied. A comparison of design values between the proposed method and the conventional method has been discussed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.1
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• pp.26-31
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• 2000

This study was carried out to obtain the basic information for breeding cold-tolerant rice varieties with high yield-productivity through wide crosses between indica and japonica rice. Genetic analysis was conducted using 55 F$_1$s obtained from half-diallel crosses among eleven cultivars of various origin including indica and japonica rice. Screening for cold tolerance was done with cold-water irrigation after transplanting until ripening stage. Both general combining ability (GCA) and specific combining ability (SCA) effects were highly significant in all characters associated with dry matter accumulation at 30 and 50days after cold-water irrigation (DAC). The variance of GCA was much larger than that of SCA in plant height, shoot dry weight per plant (DWP), crop growth rate (CGR) and cold-water response index (CRI) of these characters except CRI of shoot dry weight per plant. The DWP, CGR and CRI of these characters of Gaochan 102, Tong88-7 and TR22183 were markedly higher than those of the others. GCA effects of these varieties on DWP, CGR and their CRI were also higher than those of the others, indicating that they are useful as promising parents for breeding cold-tolerant varieties. Analysis of genetic parameters for 11$\times$11 half-diallel F$_1$s revealed that inter-locus gene interaction were concerned in the expression of plant height at 50 DAC, CRI of DWP at 50 DAC, and CRI of CGR, and that intra-locus gene interaction for plant height and the other characters were partial dominance and over-dominance, respectively. Narrow-sense heritability (h$^2$$_{N}$) was the highest in plant height as 0.729, and the lowest in CRI of DWP at 30 DAC as 0.048, suggesting that selection for cold tolerance will be quite effective in case that the selection criterion is the performance itself.f.

• Journal of Forest and Environmental Science
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• v.35 no.4
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• pp.232-239
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• 2019

This study was carried out to establish an efficient selection condition for cold-tolerant individuals among evergreen Quercus spp. To select higher cold-tolerant individuals among four species of evergreen Quercus spp. (Q. acuta, Q. glauca, Q. myrsinaefoila, and Q. salicina), an-year-old seedlings of each species were exposed to low temperature in serial, and then examined for the death of cell tissue and the surface temperature was monitored. It was shown that the higher numbers of seedlings of Q. myrsinaefolia were survived than the others when those seedlings are exposed to cold stress. Thus, selection of the cold tolerant individuals was conducted on Q. myrsinaefolia seedlings. The limit low temperature condition for selecting cold-tolerant individuals was -6℃ for 24 hrs because no seedling of Q. myrsinaefolia was survived after exposed to -7℃ for 24 hours. It was shown that the leaf surface temperature of the selected individuals was higher than those of the non-selected individuals when they were exposed to cold-stress while monitoring them using thermal graphic camera. The results in this study can be used for expanding afforestation area of tree species of the warm temperate zone for preparation of climate change.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2000.10a
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• pp.86-89
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• 2000

In cold forging by multi stage former, crowing process is important process for the high production rate and automation of forging process. But various cropping defects occur in cropping process such as orthogonality, ovality and unevenness, etc. These defects have harmful effects on the dimensional tolerance of products and tool life. So in this study, the cropping experiment is performed to examine influence of cropping process variables(clearance, cutting velocity, H/D) on occurrence of crowing defects and optimum process variables are selected, and then FE analysis is performed to verify influence of these defects on dimensional tolerance and tool life.

• Journal of Bio-Environment Control
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• v.10 no.4
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• pp.244-250
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• 2001

This study was conducted to determine the change in freezing tolerance of Chinese cabbage (Brassica campestris L. ssp. penkinensis). Four-week old seedlings grown in a greenhouse at $25^{\circ}C$$\pm$0.5 were transferred to 5, 10 or 15$^{\circ}C$ condition for acclimation for one day, and then transferred to a lethal temperature of -3$^{\circ}C$ condition. Optimal acclimation temperatures were 5$^{\circ}C$ and 1$0^{\circ}C$. Freezing tolerance of leaf tissues was investigated during a week-long cold stress. Development of freezing tolerance was shown by survival ratio, and amounts of total RNA, soluble protein and soluble sugar. LT$_{50}$ value increased from -3 to -4$^{\circ}C$ after 5 days acclimation at 5 or 1$0^{\circ}C$, and this was considered to be the first indication of enhanced freezing tolerance. Plants, cold acclimated for five days, reached to a survival ratio of 60%. This increase in freezing tolerance was found to be associated with the increased levels of total RNA, soluble sugar and soluble protein. These metabolic changes imply the association of adjustment of growth and cell metabolism with low temperatures at the beginning of cold acclimation in chinese cabbage.e.

• The Plant Pathology Journal
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• v.28 no.1
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• pp.101-106
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• 2012

Trifloxystrobin is a strobilurin fungicide, which possesses broad spectrum control against fungal plant diseases. We demonstrated that pre-treating red pepper plants with trifloxystrobin resulted in increased plant growth and leaf chlorophyll content compared with those in control plants. Relative water content of the leaves and the survival rate of intact plants indicated that plants acquired systemic tolerance to drought stress following trifloxystrobin pre-treatment. The recovery rate by rehydration in the drought treated plant was better in those pre-treated with trifloxystrobin than that in water treated plants. Induced drought tolerance activity by trifloxystrobin was sustained for 25 days after initial application. The trifloxystrobin treated red pepper plants also had induced systemic tolerance to other abiotic stresses, such as frost, cold, and high temperature stresses. These findings suggest that applying the chemical fungicide trifloxystrobin induced systemic tolerance to certain abiotic stresses in red pepper plants.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.91-99
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• 2014

The objective of this study is to identify cold-tolerance genes in Brassica rapa. In order to acheive this goal, we analyzed a KBGP-24K oligo chip data [BrEMD (B. rapa EST and Microarray Database)] using B. rapa ssp. pekinensis inbred line 'Chiifu' under cold stress condition ($4^{\circ}C$). Among 23,929 unigenes of B. rapa, 417 genes (1.7%) were primarily identified as cold responsive genes that were expressed over 5-fold higher than those of wild type control, and then a gene which has unknown function and has full length sequence was selected. It was named BrCSR (B. rapa Cold Stress Resistance). BrCSR was transformed using expression vector pSL101 to confirm whether BrCSR can enhance cold tolerance in tobacco plants. $T_1$ transgenic tobacco plants expressing BrCSR were selected by PCR and Southern hybridization analyses, and the function of BrCSR was characterized by expression level analysis and phenotype observation under cold stress condition. The expression level of BrCSR in transgenic tobacco plants increased up to about two folds in quantitative real-time RT-PCR assay and this was very similar to Northern blot hybridization analysis. Analysis of phenotypic characteristics clearly elucidated that transgenic tobaccos expressing BrCSR were more cold tolerant than wild type control under $4^{\circ}C$ treatment. Based on these results, we conclude that the over-expression of BrCSR might be closely related to the enhancement of cold tolerance.

• Molecules and Cells
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• v.39 no.3
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• pp.250-257
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• 2016

Abiotic stresses such as drought and low temperature critically restrict plant growth, reproduction, and productivity. Higher plants have developed various defense strategies against these unfavorable conditions. CaPUB1 (Capsicum annuum Putative U-box protein 1) is a hot pepper U-box E3 Ub ligase. Transgenic Arabidopsis plants that constitutively expressed CaPUB1 exhibited drought-sensitive phenotypes, suggesting that it functions as a negative regulator of the drought stress response. In this study, CaPUB1 was over-expressed in rice (Oryza sativa L.), and the phenotypic properties of transgenic rice plants were examined in terms of their drought and cold stress tolerance. Ubi:CaPUB1 T3 transgenic rice plants displayed phenotypes hypersensitive to dehydration, suggesting that its role in the negative regulation of drought stress response is conserved in dicot Arabidopsis and monocot rice plants. In contrast, Ubi:CaPUB1 progeny exhibited phenotypes markedly tolerant to prolonged low temperature ($4^{\circ}C$) treatment, compared to those of wild-type plants, as determined by survival rates, electrolyte leakage, and total chlorophyll content. Cold stress-induced marker genes, including DREB1A, DREB1B, DREB1C, and Cytochrome P450, were more up-regulated by cold treatment in Ubi:CaPUB1 plants than in wild-type plants. These results suggest that CaPUB1 serves as both a negative regulator of the drought stress response and a positive regulator of the cold stress response in transgenic rice plants. This raises the possibility that CaPUB1 participates in the cross-talk between drought and low-temperature signaling pathways.