• Natural Product Sciences
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• v.28 no.2
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• pp.68-74
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• 2022

HemoHIM was used as a Korean traditional medicine for anti-inflammatory and antioxidant effects. However, there is no study on the effect of HemoHIM on fatigue. We examined the potential use of HemoHIM to determine whether it can induce anti-fatigue effects. Mice were administered with HemoHIM and VEH for 14 days. On the last day of treatment, mice were subjected to behavioral tests. Subsequently, their plasma and muscle were collected after the treadmill test to measure lactate, lactate dehydrogenase (LDH), ammonia, corticosterone, glycogen, and creatine kinase (CK). We found that HemoHIM moderately increased the running time (s) in the treadmill and mobility duration in the cold swimming tests. In addition, the VEH group showed a significant increase in lactate, LDH, and corticosterone levels in the plasma compared to the group that did not perform the test. However, this was moderately reduced in HemoHIM treatment. Moreover, the HemoHIM-treated group showed significant differences in LDH and glycogen levels, and showed significantly different CK levels in the muscle. HemoHIM is considered to be effective in improving fatigue, given the duration of cold swimming or running time on a treadmill. Also, HemoHIM treatment resulted in reduced concentrations of blood and muscle parameter analysis.

• YAKHAK HOEJI
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• v.58 no.5
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• pp.300-306
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• 2014

Ethanol consumption causes psychomotor alterations. Hovenia Semen seu Fructus (HS), widely distributed in Korea, China, and Japan, has been reported to have beneficial effects on acute alcohol-induced liver injury. The present study sought to assess the effects of HS extract on ethanol-induced psychomotor alterations in rats. Sprague-Dawley rats were orally (p.o.) given ethanol (4 g/kg) (ethanol group) to induce psychomotor alterations. A separate group (HS-treated groups), were treated with different dosages of HS (50, 100, and 200 mg/kg, p.o.), 30 minutes before ethanol treatment. The control group received only the vehicle (saline). Ethanol-induced psychomotor alterations were evaluated in the open-field, rota-rod, hanging wire, and cold swimming test. In addition, blood ethanol and acetaldehyde concentrations were also measured. Behavioral evaluations and blood analysis were carried out 0.5, 1, 2, 4, and 8 hours after ethanol administration. Pre-treatment of HS ameliorated ethanol-induced alterations in the open-field, rota-rod, and cold swimming test, significantly evident in 2 and 4 hours after ethanol treatment. These improvements coincided with decrease in blood ethanol and acetaldehyde concentration. Based on these results, the present study suggests that HS may have ameliorating effects against ethanol-induced psychomotor alterations.

• The Journal of Internal Korean Medicine
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• v.36 no.3
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• pp.297-307
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• 2015

Objectives: We aimed to evaluate the effect of Cervi Cornu and coenzymeQ10 on exercise and endurance capacity in rats and mice. Methods: The extract of Cervi Cornu was manufactured by the pharmacy department of Kyung Hee Oriental Medical Hospital, and CoQ10 soft cap (Ildong Pharmaceutical) was used. In total, 24 rats and 30 mice were divided into 3 groups: Control (rat=8, mouse=10), CoQ10 alone (rat=8, mouse=10), Cervi Cornu extract, and CoQ10 (rat=8, mouse=10). Ergogenic effect was evaluated by administering the Cervi Cornu extract and coenzymeQ10 to rats and measuring the time to exhaustion during treadmill running; endurance capacity was assessed by measuring cold water swimming time, serum lactate level, and serum corticosterone level in each group. At 1 week from the end of treatment, we recalculated time to exhaustion during treadmill running in rats to investigate the long-term effect of the Cervi Cornu extract and coenzymeQ10. Results: Cervi Cornu extract has long-term benefits in that it preserves the ergogenic effect caused by exercise. Cervi Cornu and coenzymeQ10 have no effect on increasing cold water swimming time in ICR mice. CoenzymeQ10 decreases the serum corticosterone level in ICR mice performing cold water swimming test. Conclusions: Cervi Cornu seems to preserve the ergogenic effect caused by exercise, but a larger study is needed to investigate effect of Cervi Cornu and coenzymeQ10 on improving endurance capacity. CoenzymeQ10 decreases serum corticosterone level and it is related with the anti-psychological fatigue effect.

• Journal of Ginseng Research
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• v.35 no.3
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• pp.331-338
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• 2011

Red ginseng (RG, the extract of Panax ginseng Meyer) has various biological and psychological activities and may also alleviate fatigue-related disorders. The present study was undertaken to evaluate what kind of fatigue red ginseng alleviate. Animals were orally administered with 50, 100, 200, 400 mg/kg of RG for 7 days. Before experiments were performed. Physiological stress (swimming, rotarod, and wire test) are behavioral parameters used to represent physical fatigue. Restraint stress and electric field test to a certain degree, induce psychological fatigue in animals. Plasma concentration of lactate and corticosterone (CORT) were also measured after these behavioral assays. RG supplementation (100 mg/kg) increased movement duration and rearing frequency of restrainted mice in comparison with control. 100 and 200 mg/kg of RG increased swimming time in cold water ($8{\pm}4^{\circ}C$) while at 100 mg/kg, RG increased electric field crossing over frequencies. 50, 100 and 200 mg/kg RG prolonged running time on the rotarod and at 100 mg/kg, it increased balancing time on the wire. RG at those doses also reduced falling frequencies. RG supplementation decreased plasma CORT levels, which was increased by stress. Lactate levels were not significantly altered. These results suggest that RG supplementation can alleviate more the damages induced by psychological than physical fatigue.

• Korean Journal of Ichthyology
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• v.21 no.3
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• pp.158-166
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• 2009

To obtain the fundamental data for the mass seedling production of grunt, Hapalogenys nitens, we investigated the influence of salinity and cold water temperature on hatching and survival of eggs and growth and survival of larvae and fry. In regards to salinity, we surveyed the hatching rate and floating rate of fertilized eggs, the floating rate and survival rate of hatching larvae, and the survival and growth of fry. In respect to cold temperature, we investigated the influence of degree of daily temperature decrease, acute temperature shock, and slow temperature decreases on the survival, feeding and swimming activities of fry. In the salinity experiment, the hatching and floating rates of fertilized eggs, and the floating and survival rates of hatching larvae, were shown to be higher in seawater than in brackish water. Growth and survival of larvae and fry were not different between seawater (25~32 psu) and brackish water (5~20 psu), but were significantly lower in freshwater. In the cold-temperature test, three tests showed that rearing of fry in cold water and acutely decreasing water temperature to less than $10^{\circ}C$ reduced the survival, feeding and swimming activities of the fry. Therefore, we concluded that low salinity (less than 32 psu) could reduce the hatching rate and survival of eggs, but the growth and survival of fry were not influenced by salinity, and cold water (less than $10^{\circ}C$) decreased metabolism of grunt. During winter, we found a low-temperature limit at $8^{\circ}C$.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.575-584
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• 2021

The critical low temperature and response of the behavioral tolerance of red sea bream Pagrus major fingerlings were determined using the continuous behavior monitoring system (CBMS). The behavior of the experimental organisms was observed by decreasing the water temperature by 2.0℃ and 4.0℃ every 12 hours and 24 hours in the range of 8.0-20.0℃. An unstable behavior pattern was observed in red seabream fingerlings exposed to water temperatures below 12.0℃, in which the swimming activity decreased and repeatedly stopped, regardless of the exposure time and water temperature fluctuation. The swimming ability of the organisms exposed to 8.0-10.0℃ decreased sharply, and the behavior of staying at the bottom of the test tank was observed. Only 50 % of the organisms survived due to the low-temperature stress, and all individuals died within six hours after the cold shock. In addition, the behavior index (BI) decreased rapidly, and the amplitude change of the coefficient of variation (CV) was found to have a greater variation than the other water temperatures (p<0.05). Low-temperature stress of red sea bream is promoted at 12.0℃, and it is interpreted as the tolerance limit, which can induce a sublethal response of the organisms exposed to cold shock of 8.0-10.0℃.

• Journal of Ginseng Research
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• v.41 no.2
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• pp.201-208
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• 2017

Background: Panax vietnamensis Ha et Grushv. or Vietnamese ginseng (VG) is a recently discovered ginseng species. Studies on its chemical constituents have shown that VG is remarkably rich in ginseng saponins, particularly ocotillol saponins. However, the psychopharmacological effects of VG have not been characterized. Thus, in the present study we screened the psychopharmacological activities of VG in mice. Methods: VG extract (VGE) was orally administered to mice at various dosages to evaluate its psychomotor (open-field and rota-rod tests), sedative-hypnotic (pentobarbital-induced sleeping test), anti-stress (cold swimming test), anxiolytic (elevated plus-maze test), and cognitive (Y-maze and passive-avoidance tests) effects. Results: VGE treatment increased the spontaneous locomotor activity, enhanced the endurance to stress, reduced the anxiety-like behavior, and ameliorated the scopolamine-induced memory impairments in mice. In addition, VGE treatment did not alter the motor balance and coordination of mice and did not potentiate pentobarbital-induced sleep, indicating that VGE has no sedative-hypnotic effects. The effects of VGE were comparable to those of the Korean Red Ginseng extract. Conclusion: VG, like other ginseng products, has significant and potentially useful psychopharmacological effects. This includes, but is not limited to, psychomotor stimulation, anxiolytic, antistress, and memory enhancing effects.

• Journal of Sasang Constitutional Medicine
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• v.20 no.3
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• pp.151-163
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• 2008

1. Objective This study was done to identify the anti-stress effect of Hyeongbangdojeok-san (HDS), Yanggyeoksanhwa-tang(YST) in Soyangin. 2. Methods Experimental animals were composed of YST, HDS+stress groups which were administered each by 200mg/kg, 400mg/kg and the Saline+stress group. On the 1st day, making the rats forced swim and on the 2nd day, applying Forced swimming test to the rats. After FST, the levels of Corticosterone in the blood were measured. For the study of learning retardation, memory ability and anxiety reaction, experimental animals were composed of YST, HDS+restraint stress groups which were administered each by 400mg/kg, no stress group and the Saline+restraint stress group. Restraint stress were applied 2 hours a day for 3 weeks. On the last day of the 3rd week, Elevated Plus Maze(EPM) was applied to the groups and Morris Water Maze(MWM) was applied to the groups for 7 days. 3. Results 1. As the results of measuring FST which reflects depression, the YST+stress group and the HDS+stress group showed significant effect in comparison with the Saline+stress group. The levels of Corticosterone in the blood were decreased only in the 400mg/kg YST+stress group. 2. As the results of measuring how long EPM which reflects anxiety reaction stayed in the open arm, there was the trend which can suppress anxiety reaction in the HDS+restraint stress group bur no statistical significance. But there was any suppression of anxiety reaction in the YST+restraint stress group. 3. According to the result of MWM, the saline+restraint stress group showed the learning retardation which means increased time arriving at goal compared to the normal group at the second and third day of measurement. On the contrary, a learning retardation was significantly decreased in the YST+restraint stress group at the third day of measurement. 4. Among the Probe trial test a memory loss occurred in the saline+restraint stress group, but memory ability was significantly increased in the YST+restraint stress group. 4. Conclusion: As the results above, Soyangin Yanggyeoksanhwa-tang has significant influence to the antidepression effect, the learning retardation, the anxiety reaction and also in the Hormone level. Hyeongbangdojeok-san has significant influence to the antidepression effect, in the Hormone level, bur not to the learning retardation and anxiety reaction. prefer to drink cold water, and who are suffering from chronic gastritis.