• The Korean Journal of Physiology and Pharmacology
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• v.25 no.5
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• pp.489-494
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• 2021

Oxaliplatin, a third-generation platinum derivative, is the mainstay of current antineoplastic medications for advanced colorectal cancer therapy. However, peripheral neuropathic complications, especially cold allodynia, undermine the life-prolonging outcome of this anti-cancer agent. Rosavin, a phenylpropanoid derived originally from Rhodiola rosea, exhibits a wide range of therapeutic properties. The present study explored whether and how rosavin alleviates oxaliplatin-induced cold hypersensitivity in mice. In the acetone drop test, cold allodynia behavior was observed from days 3 to 5 after a single injection of oxaliplatin (6 mg/kg, i.p.). Cold allodynia was significantly attenuated following rosavin treatment (10 mg/kg, i.p.). Specific endogenous 5-HT depletion by three consecutive pretreatments with parachlorophenylalanine (150 mg/kg/day, i.p.) abolished the analgesic action of rosavin; this effect was not observed following pretreatment with naloxone (opioid receptor antagonist, 10 mg/kg, i.p.). Furthermore, 5-HT_1A receptor antagonist WAY-100635 (0.16 mg/kg, i.p.), but not 5-HT₃ receptor antagonist MDL-72222 (1 mg/kg, i.p.), blocked rosavin-induced analgesia. These results suggest that rosavin may provide a novel approach to alleviate oxaliplatin-induced cold allodynia by recruiting the activity of 5-HT_1A receptors.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.24-25
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• 1988

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.22-23
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• 1988

• Applied Biological Chemistry
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• v.41 no.3
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• pp.228-233
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• 1998

The storage life of satsuma mandarin(Citrus unshiu Marc. var. miyagawa) by the various pretreatment of temperatures; non-treated, room temperature, $10,\;20^{\circ}C$ and $35^{\circ}C$ were investigated. The pretreated citrus fruits were stored at $4^{\circ}C$, 85% relative humidity. Weight loss of citrus fruits by the pretreatment at $35^{\circ}C$ for 24 hrs was the lowest among that of others. Decay ratio of $35^{\circ}C$ pretreated fruits was increased at initial stages of storage, but was maintained low level after that, compared to other treatments. After 115 days storage, firmness of fruits was lowered by the softening, and decayed fruits were occurred increasingly. Ethylene evolution was increased between $55{\sim}65$ days after storage, and the amount was increasing rapidly after 115 days. It seemed to be derived from decayed fruits and physiological activities. $CO_2$ content in fruit was decreased at initial stages of storage, but was increased between $55{\sim}100$ days during storage periods. Acid content, soluble solids, total sugar and vitamin C were reduced gradually during cold storage, but the difference among treatments was not so great. Pretreated fruits at $35^{\circ}C$ for 24 hrs before cold storage was effective on preventing from weight loss and respiration ratio. Optimum storage period of early variety of Satuma mandarin was regarded for 100 days on the basis of appeareance and taste.

• Food Science and Preservation
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• v.20 no.3
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• pp.317-322
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• 2013

This study was conducted to investigate the effects of pretreatment and different packaging methods on the physicochemical properties of cold vacuum-dried peaches. All the dried peach samples were stored such as $N_2$ gas substitution, vacuum and passive packaged with polyethylene (PE) film and oriented polypropylene (OPP)/aluminum (Al)/PE film at $40^{\circ}C$ for 50 days. The weight change, pH, soluble solids content, titratable acidity, soluble solid-acid ratio (SS/TA), delta E, browning degree and phenolic compounds were analyzed. The weight change and pH were lower in the 0.1% vitamin C-treated group and were significantly lowest in the vacuum-treated OPP/AL/PE. The soluble solids content and the SS/TA were higher in the non-treated groups than in the vitamin C-treated groups. According to the packaging methods, the $L^*$ values were higher in the vacuum, $N_2$ gas and passive package, in that order. In addition, the browning degree and the delta E value were lower in the pretreated groups and significantly lowest in the vacuum-treated OPP/AL/PE with 0.1% vitamin C group. The phenolic compounds were high for the pretreated groups, according to the packaging methods (vacuum > $N_2$ gas> passive), and the OPP/AL/PE was significantly higher than the PE. These results suggest that different packaging materials and pretreatment methods affected the quality of the dried peaches, and the vacuum-OPP/AL/PE film packaging group showed a high quality.

• Journal of the Korean Wood Science and Technology
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• v.30 no.3
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• pp.109-115
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• 2002

Mycelial growth of L. edodes by pretreatments of softwood was studied on a sawdust medium. The sawdust used was from the following softwood species : Larix leptolepis, Pinus densiflora and Pinus koraiensis. The pretreatment consisted of cold-water (48 h), hot-water (3 h) and steam extractions (3 h) at a ratio of 500 g : 3,000 mL (sawdust : distilled water). The sawdust medium was a mixture of 76% sawdust, 20% rice bran, 3% glucose, 0.4% potassium nitrate and 0.6% calcium carbonate. Following sawdust pretreatments proved most suitable : L. leptolepis (steam extraction), P. densiflora (hot-water extraction) and P. koraiensis (hot-water extraction). Mycelial growth on P. koraiensis sawdust increased in proportion to an increase in hot-water extraction time. Mycelial growth was optimum on the sawdust extracted for 12 hours, hot-water extraction beyond this period proved unsuitable. With the exception of P. densiflora at 100 ㎍/mL, antifungal activity occurred in every sample. Maximum inhibition of mycelial growth was obtained from following concentration of hot-water extractives : P. densiflora (10⁴ ㎍/mL) and P. koraiensis (10⁴ ㎍/mL). This study has provided useful preliminary information for the cultivation of L. edodes.

• Horticultural Science & Technology
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• v.17 no.2
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• pp.138-140
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• 1999

These studies were carried out to investigate the effects of cold pretreatment, dark condition, and Ficoll on the callus formation and organ differentiation in anther culture of carnation for the purpose of settling the techniques of anther culture of 'Rony' carnation. Diameter of flower buds containing anthers with microspores before or after uniuncleated stage was between 4 and 6 mm. A high percentage of callus formation and responding anthers were achieved by low temperature pretreatment at $4^{\circ}C$ for 7 days. Callus formation was promoted under dark condition. Roots were differentiated from calli formed under darkness. Ficoll didn't promote callus formation but promoted differentiation of shoots and roots.

• Korean Journal of Microbiology
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• v.38 no.1
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• pp.45-49
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• 2002

Bradyrhizobium japonicum is a soil bacterium with a unique ability to infect the roots of leguminous plants and establish a nitrogen-fixing symbiosis, which has been used as a microbial manure. In this study, we examined the stress response after pretreatment of cells with cold temperature. When pre-treated with cold temperature ($4^{\circ}C$) for 16 hr, B. japonicum increased the viability in subsequent stress-conditions such as alcohol, $H_2O_2$, heat, and dehydration. For cold adpatation, cultured B. japonicum was exposed to $4^{\circ}C$. Upon subsequent exposure to various conditions, the number of adapted cells pretreated by cold adaptation was 10-1000 fold higher than that of non-adaptated ones. It appeared de novo protein synthesis occurred during adaptation, because a protein synthesis inhibitor, chloramphenicol abolished the increased stress tolerance. By using a degenerate PCR primer set, a csp homolog was amplified from B. japonicum genome and sequenced. The deduced partial amino acid sequence of the putative Csp (Cold shock protein) shares a significant similarity with known Csp proteins of other bacteria.

• Archives of Pharmacal Research
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• v.23 no.6
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• pp.620-625
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• 2000

The mechanisms of liver injury from cold storage and reperfusion are not completely under-stood. The aim of the present study was to investigate whether the inactivation of Kupffer cells (KCs) by gadolinium chloride ($GdCl_3$) modulates ischemia-reperfusion injury in the rat liver. Hepatic function was assessed using an isolated perfused rat liver model. In livers subjected to cold storage at $4^{\circ}C$ in University of Wisconsin solution for 24 hrs and to 20 min rewarm-ing ischemia, oxygen uptake was markedly decreased, Kupffer cell phagocytosis was stimulated, releases of purine nucleoside phosphorylase and lactate dehydrogenase were increased as compared with control livers. Pretreatment of rats with $GdCl_3$) , a selective KC toxicant, suppressed kupffer cell activity, and reduced the grade of hepatic injury induced by ischemia-reperfusion. While the initial mixed function oxidation of 7-ethoxycoumarin was not different from that found in the control livers, the subsequent conjugation of its meta-bolite to sulfate and glucuronide esters was suppressed by ischemia-reperfusion, CdCl$_3$restored sulfation and glucuronidation capacities to the level of the control liver. Our findings suggest that Kupffer cells could play an important role in cold/warm ischemia-reperfusion hepatic injury.

• Environmental Engineering Research
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• v.23 no.2
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• pp.159-163
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• 2018

The feasibility of applying coagulation-integrated microfiltration (MF) as a pretreatment for an ultrafiltration (UF) feed in oily wastewater treatment was investigated. The effects of different coagulants on oil removal rates from wastewater were studied. The maximum oil removal rate of 82% was obtained after coagulation with 130 mg/L of polyaluminium chloride (PAC). UF flux reached $95L/(m^2{\cdot}h)$ with coagulation-integrated MF as pretreatment. This value was 2.5 times higher than that flux obtained without pretreatment. The value of UF flux increased as the transmembrane pressure (TMP) and cross-flow velocity (CFV) of the UF module increased. UF flux gradually increased when TMP and CFV exceeded 0.4 MPa and 3 m/s, respectively, because of concentration polarization and membrane fouling stabilization. Chemical oxygen demand reduction and oil removal rate reached 95.2% and 98.5%, respectively, during integrated membrane processing with a PAC concentration of 130 mg/L, TMP of 0.4 MPa, and CFV of 3 m/s for UF. In addition, sequentially cleaning the fouling membrane with NaOH and $HNO_3$ aqueous solutions caused UF flux to recover to 90%. These encouraging results suggested that the hybrid integrated membrane process-based coagulation and MF + UF are effective approaches for oily wastewater treatment.