• Journal of Mushroom
• /
• v.18 no.1
• /
• pp.20-28
• /
• 2020

This study was conducted to determine the antioxidant, nitrite scavenging, melanin tyrosinase inhibitory, and melanogenesis inhibitory activities of fermented Coix lacryma-jobi L. var. mayuen Stapf. by Hericium erinaceum (Bull.: Fr.) mycelial hot water extract (FCLHE). Additionally, we analyzed β-glucan and ergosterol contents in FCLHE and C. lacryma-jobi hot water extract (CLHE). The ergosterol and β-glucan contents in FCLHE were 740.2 mg% and 245.3 mg%, respectively, whereas these components were not detected in CLHE. FCLHE showed higher cell viability than CLHE. When B16F10 cells were treated with 500 ㎍/mL each of CLHE and FCLHE, the FCLHE treated cells produced 8.9 uM nitric oxide (NO), which was lower than that produced by CLHE treated cells (10.6 uM). The FCLHE treated cells showed significantly greater tyrosinase inhibition and melanin production at all tested concentrations than when compared to the CLHE treated group. Antioxidant parameters such as DPPH and ABTS radical scavenging activities were higher in FCLHE than in CLHE. These results suggest that FCLHE can be used as a raw material for functional foods, for food additives, and in the cosmetic industry.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.64 no.4
• /
• pp.452-458
• /
• 2019

Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 ℃ under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC₅₀], 68.9 ± 4.1 g/mL; and ABTS, IC₅₀, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.

• Korean Journal of Soil Science and Fertilizer
• /
• v.9 no.4
• /
• pp.245-250
• /
• 1976

A field study was conducted to find out the optimum level of NPK fertilizers for pearl barley (Coix Lacryma-Jobi L.). The results are sumarized as follows. 1. When all of NPK fertilizers were increased together as 0-0-0, 6-3-3, 12-6-6-6 and 18-9-9 kg/10a of NPK, the grain yield also increased lineally. 2. When the level of two other elements were fixed at 12kg/10a for N and 6kg/10a for P and K, maximum yields were obtained at 12kg/10a of N and 6kg/10a of P and K. 3. Accordingly, with present results, it is difficult to point out the optimum levels of NPK. It seemed necessary to conduct another trial with expanded NPK levels. However it can be pc pointed out that the yield of pearl barley can be increased with higher levels of NPK fertilizers than 18-9-9 kg/10a at the similar soil used for present study. 4. The antagonism phenomena were observed between N and K in plant's uptake.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.5
• /
• pp.413-418
• /
• 1992

The higher fertilizer application level and frequency of additional fertilizer increase the higher the growing characters of plant height, No. of leaves, No. of stem per plant, and No. of ears per main stem, etc., appeared. The yield characters of No. of grains per plant, percentage of threshing, weight of 1 $\ell$, weight of 100 grains and seed yield per 10a appeared high both in used N 14kg, 40% as the basic dressing, 30% as the 1st top dressing, 20% as the 2nd top dressing and 10% as the 3rd top dressing, and in used N 18kg as the total amount of basic dressing. Dispersion analysis showed the difference of significant level according to the interactions, among fertilizer application levels, among fertilizational methods, and between fertilizer application and fertilizational method.

• Korean Journal of Organic Agriculture
• /
• v.22 no.2
• /
• pp.321-334
• /
• 2014

This study was conducted to identify plant growth promoting effects of native plant resources and medicinal plants. 65 kinds of plant extracts from 64 species were evaluated based on two rice(Oryza sativa) seedling bioassays for gibberellins and gibberellin-like substances. 12 extracts of Artemisia princeps stem and leaf, Cirsium japonicum var. maackii whole plant, Rhododendron mucronulatum branch and leaf, Phragmites communis stem, Coix lacryma-jobi var. mayuen whole plant, Lespedeza bicolor branch and leaf, Hydrangea serrata f. acuminata whole plant, Phlomis umbrosa whole plant, Glycyrrhiza uralensis Rhizome, G. uralensis stem and leaf, Angelica gigas root, and Cnidium officinale rhizome showed growth promotion of rice seedlings. Our results suggested that the parts of these plants could be the potential sources as farm-made liquid fertilizers for plant growth promotion.

• Korean Journal of Medicinal Crop Science
• /
• v.8 no.1
• /
• pp.79-82
• /
• 2000

This study was carried out to investigate the effect of seed disinfectant on leaf blight and smut in adlay. Leaf blight and smut disease in adlay are known to be seed borne. Bjpolan's cojds, pathogen causing leaf blight in adlay, was detected from 32. 7 to 45. 7 % on seeds collected from adlay cultivated farms in Yonchon province. Periods of seed infestation are $0{\sim}7$ days on the variety 'yulmu 1' and $0{\sim}20$ days on 'Yonchon jaerae'. Distribution ratio of seed disinfestation periods was that no disinfestation, $1{\sim}5$days, $6{\sim}10$days and more 11days were 20%, 20%, 45% and 15%, respectively. Frudioxonil W. S. treatment was effective on inhibition of leaf blight and smut disease when treated with infested seeds ; that is, disease control values of the fungicide are 97 and 93.4 percentage, repectively.

• Korean Journal of Medicinal Crop Science
• /
• v.8 no.1
• /
• pp.74-78
• /
• 2000

This study was carried out to select the effective insecticides for control of Ostrinia furnacalis(Guenee) in Yonchon, northern part of Kyunggi province, where is main producing region of adlay in Korea. The Asian corn borer in adlay field had three generations a year. The first stage of adult activity was higher from late May to middle June, the second from late July to early August and the third late September. Based on difference in control value, the relative ranks of some insecticides were lambda cyhalothrin EC > cartap hydrochloride GR > carbaryl EC. In control value by different application timing of Lambda cyhalothrin EC, yields were significantly different, that is, twice applications on second generation showed the control value of $90.6%{\sim}93.5%$. Unhulled grain yield on Lambda cyhalothrin EC treated plot on second generation was higher than that in untreatred plot by 82%.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.2
• /
• pp.175-179
• /
• 1994

This experiment was carried out to investigate the effect of improved NPK fertilizer and ameliorator application methods on job's tears yield and soil properties in the newly-reclaimed land from 1985 through 1988. Job's tears height of integrated improvement plot was higher than the other plots, and increased from the 1st and the 2nd year, but decreased from the 3rd year when soil conditioner was not applied. Average yield of the crop in the integrated improvement plot was 2.16 t /ha being increased by 49% more than the control plot, and the yield was the greatest in order of integrated improvement>subsoiling>phosphate>lime>compost>control plot. Soil bulk density and hardness of the topsoil decreased to the 3rd year, but increased in the 4th year. Soil pH of the topsoil was not changed significantly by different soil depths and cultivated years.

• Advances in Traditional Medicine
• /
• v.9 no.1
• /
• pp.20-38
• /
• 2009

The objectives of this study were to determine the effect of herb formula HDDM, a modification of Huangdan decoction that has been shown to be effective in the treatment of glomerulonephritis and chronic renal failure, on the blood glucose levels in multiple low doses (MLD; 50 mg/kg for five consecutive days) of streptozotocin (STZ)-treated female B6C3F1 mice. Initial studies were performed to compare diabetes induction in five strains (e.g., B6C3F1, NOD, CD-1, C3H/HeN and C57BL/6) of mice by MLD-STZ, and immune changes following the treatment. The results suggested that the order of susceptibility to diabetes induction was NOD $\approx$ CD-1 > B6C3F1 $\approx$ C3H > C57BL/6. Furthermore, STZ modulation of T cell development, differentiation and activation might play a role in diabetes induction by MLD-STZ treatment. MLD-STZ-induced diabetes in female B6C3F1 mice was moderate, which allowed the evaluation of drug-induced protection or exacerbation of diabetes to be performed. As such, modulation of blood glucose by HDDM, which consisted of Da Huang (Radix Et Rhizoma Rhei), Huang Qi (Radix Astragali Seu Hedysari), Dan Shen (Radix Salviae Miltiorrhizae), Yin Yang Huo (Herba Epimedii), Yi Yi Ren (Semen Coicis or Coix lacryma-jobi), Mai Dong (Radix Ophiopogonis) and Shan Zhu Yu (Fructus Corni), was evaluated in MLD-STZ-treated female B6C3F1 mice. The results suggested that HDDM could lower the blood glucose levels, but it had no immunomodulatory activities. Additionally, HDDM-treated mice exhibited improved glucose tolerance. In conclusion, these studies have suggested that MLD-STZ-induced diabetes in female B6C3F1 mice is a useful model to evaluate drug modulation of diabetes, and that the herb formula HDDM possesses anti-diabetic effects.

• Korean Journal of Food Science and Technology
• /
• v.47 no.2
• /
• pp.246-253
• /
• 2015

In this study, we evaluated the lipoprotein lipase (LPL) inhibitory activity of 11 water extracts derived from Cinnamomum cassia Blume, Sarcodon aspratus, Cordyceps militaris, Crataegus pinnatifida Bunge, Corni fructus, Allium cepa, Coix lacryma-jobi, Plantago asiatica L., Lentinus edodes, Rosa rugosa, and Foeniculum fructus. The results of the LPL secretion and activity assay showed Sarcodon aspratus (NE) extract have an LPL secretion inhibitory acitivity. The cause of reduction in LPL secretion after NE treatment was investigated using molecular biology methods. NE treatment affected the LPL content in cells, but did not affect LPL mRNA expression. It also increased the mRNA expression level of sortilin-related receptor LDLR class A (SorLA), a receptor that induces endocytosis and intracellular trafficking of LPL. Finally, cell fractionation revealed that NE treatment induced the expression of CCAAT-enhancer-binding protein beta ($C/EBP{\beta}$), a SorLA transcription factor, in the nuclei of 3T3-L1 adipocytes. These results show that NE's anti-obesity effect involves inhibition of LPL secretion through $C/EBP{\beta}$-mediated induction of SorLA expression.