• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.11C
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• pp.1058-1065
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• 2006

In this paper, we propose a new motion vector coding algorithm suitable for MPEG-4 Part 10 video coding standard. In the proposed algorithm, the amount of the motion for a given video sequence is Predicted by using a characteristic of the motion vector distribution for the neighboring blocks as well as the MB_type, which is the syntax element in the standard. And one of the independent coding and the combined coding methods is adaptively employed to compress the motion vector difference. Simulation results show that the proposed algorithm outperforms the conventional methods without additional memory and calculations.

• The Transactions of the Korea Information Processing Society
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• v.7 no.2S
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• pp.701-710
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• 2000

This paper presents a new method for an efficient coding of very low bit-rate color video based on adaptive wavelet transform. Our approach reveals that the coding process works more efficiently if the quantized wavelet coefficients are preprocessed by a mechanism exploiting the redundancies in the wavelet subband structure. Thus, we focuses optimized activity of coding part, and exhaustive overlapped block motion compensation is utilized to ensure coherency in motion compensated error frames, and raised cosine window is applied. The horizontal and vertical components of motion vectors are encoded separately using adaptive arithmetic coding while significant wavelet coefficients are encoded in bit-plane order using adaptive arithmetic coding. On average the proposed codec exceeds H.263 and ZTE in peak signal-to-noise ratio by as much as 2.07 and 1.38dB at 28 kbits, respectively. Fore entire sequence coding, 3DWCVC method is superior to H.263 and ZTE by 0.35 and 0.71dB on average, respectively.

• Journal of Broadcast Engineering
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• v.7 no.3
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• pp.238-247
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• 2002

In this paper, we propose an object-based stereo sequence compression technique using disparity-motion vector relationship. The proposed method uses the coherence of motion vectors and disparity vectors in the left and right Image sequences. After two motion vectors and one disparity vector ate computed using FBMA(Fixed Block Matching Algorithm), the disparity vector of the current stereoscopic pall is computed by disparity-motion vector relationship with vectors which are previously estimated. Moreover, a vector regularization technique is applied in order to obtain reliable vectors. For an object-based coding. the object is defined and coded in terms of layers of VOP such as in MPEG-4. we present a method using disparity and motion vector relationship for extending two-frame compensation into three-frame compensation method for prediction coding of B-VOP. The proposed algorithm shows a high performance when comparing with a conventional method.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.27-36
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• 2013

We isolated and functionally characterized a Dendrobium Actin1 (DmACT1) promoter that drives strong gene expression in the orchid genus Dendrobium. A genomic fragment containing the region 3227 bp upstream of the coding region of DmACT1 was obtained by inverse PCR. Detailed comparison of the full-length cDNA and genomic sequences revealed that DmACT1 has a 1374 bp first intron in the 5' UTR. However, the 5' flanking sequences upstream of the coding region showed no obvious sequence similarities compared to those of known promoters, including plant actin promoters. Serial deletion constructs of the 5' flanking region from the translation initiation codon were fused to the coding sequence of a GUS/luciferase fusion reporter to identify the regulatory elements necessary for promoter activity. Transient assays in the flowers of Dendrobium revealed that the 5' UTR-intron greatly enhanced promoter activity. Moreover, the DmACT1 promoter with its 5' UTR-intron yielded approximately 10-fold higher reporter activity than the rice Act1 promoter-intron. Our data suggest that the DmACT1 promoter with its 5' UTR-intron is a useful tool for strong expression of transgenes in Dendrobium orchids.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.283-285
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• 2019

The draft genome sequencing for Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261), isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.85 Mbp with G + C content of 54.3%, and included a total of 4,566 protein-coding genes, 3 rRNA genes, 48 tRNA genes, 3 non-coding RNA genes, and 67 pseudo genes. In the draft genome, the strain DSW4-44 contained genes involved in the nitrogen metabolism of dissimilatory nitrate reduction to ammonium (DNRA) and denitrification, which were not found other strains in the genus Pelagicola.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.480-482
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• 2018

The draft genome sequencing for Zhongshania marina $DSW25-10^T$, isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.08 Mbp with G + C content of 49.0%, and included a total of 3,702 protein-coding genes, 3 rRNA genes, 39 tRNA genes, 4 non-coding RNA genes, and 36 pseudogenes. In addition, the metabolic pathways of aliphatic and aromatic compounds were identified. In light of these metabolic pathways, Zhongshania marina $DSW25-10^T$ is expected to be a useful bioremediation resource.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.857-864
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• 2018

Previous studies have not detected transcripts of the gene encoding anthocyanidin synthase (ANS) in white pomegranates (Punica granatum L.) and suggest that a large-sized insertion in the coding region of the ANS gene might be the causal mutation. To elucidate the identity of the putative insertion, 3887-bp 5' and 3392-bp 3' partial sequences of the insertion site were obtained by genome walking and a gene coding for an expansin-like protein was identified in these genome-walked sequences. An identical protein (GenBank accession OWM71963) isolated from pomegranate was identified from BLAST search. Based on information of OWM71963, a 5.8-Mb scaffold sequence with genes coding for the expansin-like protein and ANS were identified. The scaffold sequence assembled from a red pomegranate cultivar also contained all genome-walked sequences. Analysis of positions and orientations of these genes and genome-walked sequences revealed that the 27,786-bp region, including the 88-bp 5' partial sequences of the ANS gene, might be translocated into an approximately 22-kb upstream region in an inverted orientation. Borders of the translocated region were confirmed by PCR amplification and sequencing. Based on the translocation mutation, a simple PCR codominant marker was developed for efficient genotyping of the ANS gene. This molecular marker could serve as a useful tool for selecting desirable plants at young seedling stages in pomegranate breeding programs.

• Journal of Broadcast Engineering
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• v.9 no.3
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• pp.236-245
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• 2004

A multiview sequence CODEC with view scaiability is proposed in this paper. We define a GGOP (Group of GOP) structure as a basic coding unit to efficiently code multiview sequences. 7he proposed CODEC provides flexible GGOP structures based on the number of views and baseline distances among cameras. Multiview sequences encode consists of disparity estimation/compensation, motion estimation/compensation, residual coding and rate control and generates multiview sequence bitstream. The main bitstream is the same as an MPEG-2 mono-sequence bitstream for MPEG-2 compatibility. The auxiliary bitstream contains information concerning the remaining multiview sequences except for the reference sequences. The proposed CODEC with view scalability provides that a number of view flints are selectively determined at the receiver according to the type of display modes. The proposed multiview sequence CODEC is tested with several multiview sequences to determine its flexibility. compatibility with MPEG-2 and view scaiability. In addition, we subjectively confirm that the decoded bitstreams with view scaiability can be Properly displayed by several types of display modes. including 3D monitors.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.850-855
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• 2007

Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of leptin and its function in poultry is very poor. In this study, the duck leptin gene was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) from duck liver RNA. The cDNA fragment was inserted into the pET-28a expression vector, and the resulting plasmid was expressed in Escherichia coli BL21 (DE3). Experimental mice were given an intraperitoneal injection of 10 mg/kg leptin dissolved in phosphate buffered saline (PBS), while the control mice were injected with PBS. The effect of leptin on food intake, body weight and fatty deposition in mice was detected. Sequence analysis revealed that duck leptin had a length of 438 nucleotides which encoded a peptide with 146 amino acid residues. The sequence shares highly homology to other animals. The coding sequence of duck leptin was 84 and 86% identical to human and pig leptin nucleotides sequence. Highest identity was with the rat coding sequence (95%). The identity of the amino acid sequence was 84, 82 and 96% respectively compared to that of the human, pig and rat. Results of SDS-PAGE analysis indicated that a fusion protein was specifically expressed in E. coli BL21 (DE3). The purified product was found to be biologically active during tests. Continuous administration of recombinant duck leptin inhibited food intake. Despite the decrease of food intake, leptin significantly induced body weight and fatty deposition. These changes were accompanied by a significant down-secretion of plasma glucose, cholesterol, triglyceride and insulin levels in mice. The observations provide evidence for an inhibitory effect of leptin in the regulation of food intake and for a potential role of duck leptin in the regulation of lipogenesis.

• Animal Systematics, Evolution and Diversity
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• v.26 no.3
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• pp.197-201
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• 2010

We sequenced the whole mitochondrial genome of Dendronephthya gigantea (Anthozoa: Octocorallia: Nephteidae), the first mitochondrial genome sequence report in the Family Nephtheidae. The mitochondrial genome of D. gigantea was 18,842 bp in length, and contained 14 protein coding genes (atp6 and 8, cox1-3, cytb, nd1-6 and 4L, and msh1), two ribosomal RNAs, and only one transfer RNA. The gene content and gene order is identical to other octocorals sequenced to date. The portion of the noncoding regions is slightly larger than the other octocorals (5.08% compared to average 3.98%). We expect that the information of gene content, gene order, codon usage, noncoding region and protein coding gene sequence could be used in the further analysis of anthozoan phylogeny.