• Title/Summary/Keyword: Coding Sequence

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Isolation and Characterization of the Ribosomal Protein 46 Gene in Drosophila melanogaster

    • Animal cells and systems
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    • v.2 no.1
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    • pp.113-116
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    • 1998
  • A cDNA clone coding for ribosomal protein 46 (rp46) which is a component of 60S ribosomal large subunit has been identified from Drosophila melanogaster. A cDNA clone encoding S. cerevisiae rp46 was used as a probe to screen a Drosophila larvae cDNA library. The DNA sequence analysis revealed that the cDNA coding for Drosophils rp46 contains a complete reading frame of 153 nucleotides coding for 51 amino acids. The deduced amino acid sequence showed 71-75% homology with those of other eukaryotic organisms. Northern blot analysis showed that about 1-kb rp46 transcripts are abundant throughout fly development. Whole mount embryonic mRNA in situ hybridization also showed no preferential distribution of the transcripts to any specific region. The chromosomal in situ hybridization revealed that the identified gene is localized at position 60C on the right arm of the second polytene chromosome with a possibility of single copy.

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10 GHz Multiuser Optical CDMA Based on Spectral Phase Coding of Short Pulses

  • Ruan, Wan-Yong;Won, In-Jae;Park, Jae-Hyun;Seo, Dong-Sun
    • Journal of IKEEE
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    • v.13 no.1
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    • pp.65-70
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    • 2009
  • We propose an ultrashort pulse optical code-division multiple-access (O-CDMA) scheme based on a pseudorandom binary M-sequence spectral phase encoding and decoding of coherent mode-locked laser pulses and perform a numerical simulation to analyze its feasibility. We demonstrate the ability to properly decode any of the multiple (eight) 10 Gbit/s users by the matched code selection of the spectral phase decoder. The peak power signal to noise ratio of properly and improperly decoded $8{\times}10 Gb/s$ signals could be greater than 15 for 127 M-sequence coding.

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ORF Miner: a Web-based ORF Search Tool

  • Park, Sin-Gi;Kim, Ki-Bong
    • Genomics & Informatics
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    • v.7 no.4
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    • pp.217-219
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    • 2009
  • The primary clue for locating protein-coding regions is the open reading frame and the determination of ORFs (Open Reading Frames) is the first step toward the gene prediction, especially for prokaryotes. In this respect, we have developed a web-based ORF search tool called ORF Miner. The ORF Miner is a graphical analysis utility which determines all possible open reading frames of a selectable minimum size in an input sequence. This tool identifies all open reading frames using alternative genetic codes as well as the standard one and reports a list of ORFs with corresponding deduced amino acid sequences. The ORF Miner can be employed for sequence annotation and give a crucial clue to determination of actual protein-coding regions.

A fractal coding technique for color image sequence employing non-contractive interframe mapping (비축소 프레임간 변환을 이용한 컬러 동영상 프랙탈 부호화 기법)

  • 김창수;김인철;이상욱
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.22 no.8
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    • pp.1707-1714
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    • 1997
  • This paper proposes a novel algorithm for fractal coding of image sequence, based on the CPM (Circular Prediction Mapping) and the NCIM (Non Contractive Interframe Mapping). In the CPM and the NCIM, each range block is approximated by a domain block in the adjacent frame, which is of the same size as the range block. Also, in this paepr, we propose a coding scheme of color components and an algorithm for controlling the bit rate, resepectively, for practical implementation of the fractal coder. The computer simulation results on real image sequences demonstrate that the proposed algorithm provides very promising performance at low bit-rate, below 256 Kbps.

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An Efficient Motion Vector Coding Algorithm for the Video Sequence with Slow Motion (저속 동영상에 효과적인 움직임 벡터 부호화 알고리듬)

  • Moon Yong ho;Kim Young kuk;Chang Jung hwan;Kim Jae ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.4C
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    • pp.269-275
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    • 2005
  • In this paper, we propose a new efficient motion vector coding algorithm for the video sequence with slow motion. In the proposed algorithm, the amount of motion for a given video sequence is determined by a Skip_rate parameter. The motion difference for slow motion is encoded with a combined codeword which is generated from the conventional codewords. The simulation results show that the proposed algorithm achieves approximately $15\%$ bits gain compared to the conventional methods. Moreover, additional memory and calculations for statistical observation are not required in the proposed algorithm.

A Robust DNA Watermarking in Lifting Based 1D DWT Domain (Lifting 기반 1D DWT 영역 상의 강인한 DNA 워터마킹)

  • Lee, Suk-Hwan;Kwon, Ki-Ryong;Kwon, Seong-Geun
    • Journal of the Institute of Electronics and Information Engineers
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    • v.49 no.10
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    • pp.91-101
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    • 2012
  • DNA watermarking have been interested for both the security of private genetic information or huge DNA storage information and the copyright protection of GMO. Multimedia watermarking has been mainly designed on the basis of frequency domain, such as DCT, DWT, FMT, and so on, for the robustness and invisibility. But a frequency domain watermarking for coding DNA sequence has a considerable constraint for embedding the watermark because transform and inverse transform must be performed without completely changing the amino acid sequence. This paper presents a coding sequence watermarking on lifting based DWT domain and brings up the availability of frequency domain watermarking for DNA sequence. From experimental results, we verified that the proposed scheme has the robustness to until a combination of 10% point mutations, 5% insertion and deletion mutations and also the amino preservation and the security.

An Efficient Receiver Structure Based on PN Performance in Underwater Acoustic Communications (수중음향통신에서 PN 성능 기반의 효율적인 수신 구조)

  • Baek, Chang-Uk;Jung, Ji-Won
    • Journal of Navigation and Port Research
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    • v.41 no.4
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    • pp.173-180
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    • 2017
  • Underwater communications are degraded as a result of inter symbol interference in multipath channels. Therefore, a channel coding scheme is essential for underwater communications. Packets consist of a PN sequence and a data field, and the uncoded PN sequence is used to estimate the frequency and phase offset using a Doppler and phase estimation algorithm. The estimated frequency and phase offset are fed to a coded data field to compensate for the Doppler and phase offset. The PN sequence is generally utilized to acquire the synchronization information, and the bit error rate of an uncoded PN sequence predicts the performance of the coded data field. To ensure few errors, we resort to powerful BCJR decoding algorithms of convolutional codes with rates of 1/2, 2/3, and 3/4. We use this powerful channel coding algorithm to present an efficient receiver structure based on the relation between the bit error of the uncoded PN sequence and coded data field in computer simulations and lake experiments.

Nucleotide Sequence on Upstream of the cdd Locus in Bacillus subtilis

  • JONG-GUK KIM;KIM, KYE-WON;SEON-KAP HWANG;JOO-WON SUH;BANG-HO SONG;SOON-DUCK HONG
    • Journal of Microbiology and Biotechnology
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    • v.5 no.3
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    • pp.125-131
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    • 1995
  • A 3, 346 bp of the cdd upstream region in Bacillus subtilis was sequenced from the pSO1 (Song BH and J Neuhard. 1989. Mol. Gen. Genet 216: 462-468) and sequence homology was searched to the known genes in Genbank and European Molecular Biology Laboratory databanks. Five complete and one truncated putative coding sequences deduced from the nucleotide sequence were found through the ORF searching by Genetyx and Macvector software, and one of them was identified as the dgk (diacylglycerol kinase) gene and another, a truncated one, as the phoH (phosphate starvation-inducible gene) gene. The B. subtilis dgk gene, having a role for response to several environmental stress signals, revealed an open reading frame of 134 amino acids with 43.1% of sequence identity to the Streptococcus mutans dgk gene. The carboxy terminal 59 residues of the truncated phoH gene showed 52.7% and 34.5% of sequence identity in amino acids with the corresponding genes of Mycobacterium leprae and Escherichia coli. The four remaining coding sequences consisting of 115, 421, 91, and 91 residues were thought to be unknown ORFs because they have no significant similarity to known genes.

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Identification of Viral Taxon-Specific Genes (VTSG): Application to Caliciviridae

  • Kang, Shinduck;Kim, Young-Chang
    • Genomics & Informatics
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    • v.16 no.4
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    • pp.23.1-23.5
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    • 2018
  • Virus taxonomy was initially determined by clinical experiments based on phenotype. However, with the development of sequence analysis methods, genotype-based classification was also applied. With the development of genome sequence analysis technology, there is an increasing demand for virus taxonomy to be extended from in vivo and in vitro to in silico. In this study, we verified the consistency of the current International Committee on Taxonomy of Viruses taxonomy using an in silico approach, aiming to identify the specific sequence for each virus. We applied this approach to norovirus in Caliciviridae, which causes 90% of gastroenteritis cases worldwide. First, based on the dogma "protein structure determines its function," we hypothesized that the specific sequence can be identified by the specific structure. Firstly, we extracted the coding region (CDS). Secondly, the CDS protein sequences of each genus were annotated by the conserved domain database (CDD) search. Finally, the conserved domains of each genus in Caliciviridae are classified by RPS-BLAST with CDD. The analysis result is that Caliciviridae has sequences including RNA helicase in common. In case of Norovirus, Calicivirus coat protein C terminal and viral polyprotein N-terminal appears as a specific domain in Caliciviridae. It does not include in the other genera in Caliciviridae. If this method is utilized to detect specific conserved domains, it can be used as classification keywords based on protein functional structure. After determining the specific protein domains, the specific protein domain sequences would be converted to gene sequences. This sequences would be re-used one of viral bio-marks.

3D video coding for e-AG using spatio-temporal scalability (e-AG를 위한 시공간적 계위를 이용한 3차원 비디오 압축)

  • 오세찬;이영호;우운택
    • Proceedings of the IEEK Conference
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    • 2003.11a
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    • pp.199-202
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    • 2003
  • In this paper, we propose a new 3D coding method for heterogeneous systems over enhanced Access Grid (e-AG) with 3D display using spatio-temporal scalability. The proposed encoder produces four bit-streams: one base layer and enhancement layer l, 2 and 3. The base layer represents a video sequence for left eye with lower spatial resolution. An enhancement layer l provides additional bit-stream needed for reproduction of frames produced in base layer with full resolution. Similarly, the enhancement layer 2 represents a video sequence for right eye with lower spatial resolution and an enhancement layer 3 provides additional bit-stream needed for reproduction of its reference pictures with full resolution. In this system, temporal resolution reduction is obtained by dropping B-frames in the receiver according to network condition. The receiver system can select the spatial and temporal resolution of video sequence with its display condition by properly combining bit-streams.

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