• Biomolecules & Therapeutics
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• v.29 no.2
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• pp.234-247
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• 2021

We used a heterozygous gene deletion library of fission yeasts comprising all essential and non-essential genes for a microarray screening of target genes of the antifungal terbinafine, which inhibits ergosterol synthesis via the Erg1 enzyme. We identified 14 heterozygous strains corresponding to 10 non-essential [7 ribosomal-protein (RP) coding genes, spt7, spt20, and elp2] and 4 essential genes (tif302, rpl2501, rpl31, and erg1). Expectedly, their erg1 mRNA and protein levels had decreased compared to the control strain SP286. When we studied the action mechanism of the non-essential target genes using cognate haploid deletion strains, knockout of SAGA-subunit genes caused a down-regulation in erg1 transcription compared to the control strain ED668. However, knockout of RP genes conferred no susceptibility to ergosterol-targeting antifungals. Surprisingly, the RP genes participated in the erg1 transcription as components of repressor complexes as observed in a comparison analysis of the experimental ratio of erg1 mRNA. To understand the action mechanism of the interaction between the drug and the novel essential target genes, we performed isobologram assays with terbinafine and econazole (or cycloheximide). Terbinafine susceptibility of the tif302 heterozygous strain was attributed to both decreased erg1 mRNA levels and inhibition of translation. Moreover, Tif302 was required for efficacy of both terbinafine and cycloheximide. Based on a molecular modeling analysis, terbinafine could directly bind to Tif302 in yeasts, suggesting Tif302 as a potential off-target of terbinafine. In conclusion, this genome-wide screening system can be harnessed for the identification and characterization of target genes under any condition of interest.

• Journal of Food Hygiene and Safety
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• v.35 no.6
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• pp.607-617
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• 2020

Human bio-monitoring (HBM) data is a very important resource for tracking total exposure and concentrations of a parent chemical or its metabolites in human biomarkers. However, until now, it was difficult to execute the integration of different types of HBM data due to incompatibility problems caused by gaps in study design, chemical description and coding system between different sources in Korea. In this study, we presented a standardized code system and HBM knowledge model (KM) based on relational database modeling methodology. For this purpose, we used 11 raw datasets collected from the Ministry of Food and Drug Safety (MFDS) between 2006 and 2018. We then constructed the HBM database (DB) using a total of 205,491 concentration-related data points for 18,870 participants and 86 chemicals. In addition, we developed a summary report-type statistical analysis program to verify the inputted HBM datasets. This study will contribute to promoting the sustainable creation and versatile utilization of big-data for HBM results at the MFDS.

• Journal of the Korea Convergence Society
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• v.12 no.8
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• pp.215-228
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• 2021

The purpose of this qualitative study was to identify cultural factors among older Americans that could influence them to accept new welfare technologies. This study also explored how social and cultural-based plans could increase the acceptability of welfare technologies for improving the quality of life of older adults in the future. In-depth interviews were conducted with ten professionals who work with older adults. The collected interview data were subsequently analyzed using a two-cycle open coding process. The data analysis generated 29 codes that were organized into 7 primary codes, or categories, and 22 secondary codes nested within the primary codes. Several themes were identified: individualism, family-oriented culture, pragmatism, low-context culture, privacy, fun-seeking culture, and a less hierarchical culture. These findings will inform the development of a future survey to examine the relationship between older adults' intentions when using technology and socio-cultural factors in community settings. In order to explore the different impact levels of the cultural factors found in this study, the future study will need to include measures for identifying socio-cultural variations among individuals in one country or across countries.

• The Journal of the Korea Contents Association
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• v.21 no.5
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• pp.488-498
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• 2021

This study applied Flipped Learning teaching and learning method to Basics Cooking Practice Subject using YouTube. The purpose of this study is to investigate whether the curriculum is properly progressing by grasping the effects of before and after learning and analyzing learners' subjectivity through the learning process. The investigation period was conducted from August 01, 2020 to September 10, 2020. According to the research design of Q Methodology, it was divided into five stages: Q sample selection, P sample selection, Q sorting, coding and recruiting, conclusion and discussion. As a result of the analysis, the first type (N=5): Prior Learning effect, the second type (N=7): Simulation practice effect, and the third type (N=3): self-efficacy effect. As a result, by applying the flipped learning teaching method of the Basics Cooking Practice Subject using YouTube, positive effects such as inducing interest in the class and increasing confidence were found in active learners, but some learners lacked understanding of the system of the class operation method. However, the lack of number of training sessions compared to other subjects is considered to be a solution to be solved later.

• Journal of Broadcast Engineering
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• v.27 no.2
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• pp.216-227
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• 2022

The MPEG-I (Immersive) group is working on a standardization project for immersive video that provides 6 degrees of freedom (6DoF). The MPEG Immersion Video (MIV) standard technology is intended to provide limited 6DoF based on depth map-based image rendering (DIBR) technique. Many efficient coding methods have been suggested for MIV, but efficient transmission strategies have received little attention in MPEG-I. This paper proposes group-based adaptive rendering method for immersive video streaming. Each group can be transmitted independently using group-based encoding, enabling adaptive transmission depending on the user's viewport. In the rendering process, the proposed method derives weights of group for view synthesis and allocate high quality bitstream according to a given viewport. The proposed method is implemented through the Test Model for Immersive Video (TMIV) test model. The proposed method demonstrates 17.0% Bjontegaard-delta rate (BD-rate) savings on the peak signalto-noise ratio (PSNR) and 14.6% on the Immersive Video PSNR(IV-PSNR) in terms of various end-to-end evaluation metrics in the experiment.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.33 no.6
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• pp.257-264
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• 2021

This study established a numerical model capable of calculating the wave overtopping rate of coastal structures by nonlinear irregular waves using the FUNWAVE-TVD model, a fully nonlinear Boussinesq equation model. Here, a numerical model was established by coding the mean value approach equations of EurOtop (2018) and empirical formula by Goda (2009), and adding them as subroutines of the FUNWAVE-TVD model. The verification of the model was performed by numerically calculating the wave overtopping rate of nonlinear irregular waves on vertical wall structures and comparing them with the experimental results presented in EurOtop (2018). As a result of the verification, the numerical calculation result according to the EurOtop equation of this model was very well matched with the experimental result in all relative freeboard (R_c/H_mo) range under non-impulsive wave conditions, and the numerical calculation result of empirical formula was evaluated slightly smaller than the experimental result in R_c/H_mo < 0.8 and slightly larger than the experimental result in R_c/H_mo > 0.8. The results of this model were well represented in both the exponential curve and the power curve under impulsive wave conditions. Therefore, it was confirmed that this numerical model can simulate the wave overtopping rate caused by nonlinear irregular waves in an vertical wall structure.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.15 no.1
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• pp.56-61
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• 2022

In this paper, we classify and present AI algorithms and natural language processing methods used in chatbots. A framework that can be used to implement a chatbot is also described. A chatbot is a system with a structure that interprets the input string by constructing the user interface in a conversational manner and selects an appropriate answer to the input string from the learned data and outputs it. However, training is required to generate an appropriate set of answers to a question and hardware with considerable computational power is required. Therefore, there is a limit to the practice of not only developing companies but also students learning AI development. Currently, chatbots are replacing the existing traditional tasks, and a practice course to understand and implement the system is required. RNN and Char-CNN are used to increase the accuracy of answering questions by learning unstructured data by applying technologies such as deep learning beyond the level of responding only to standardized data. In order to implement a chatbot, it is necessary to understand such a theory. In addition, the students presented examples of implementation of the entire system by utilizing the methods that can be used for coding education and the platform where existing developers and students can implement chatbots.

• Journal of Broadcast Engineering
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• v.27 no.3
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• pp.318-331
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• 2022

With the recent development of hardware computing devices and software based frameworks, machine tasks using deep learning networks are expected to be utilized in various industrial fields and personal IoT devices. However, in order to overcome the limitations of high cost device for utilizing the deep learning network and that the user may not receive the results requested when only the machine task results are transmitted from the server, Collaborative Intelligence (CI) proposed the transmission of feature maps as a solution. In this paper, an efficient compression method for feature maps with vast data sizes to support the CI paradigm was analyzed and presented through experiments. This method increases redundancy by applying feature map reordering to improve compression efficiency in traditional video codecs, and proposes a feature map method that improves compression efficiency and maintains the performance of machine tasks by simultaneously utilizing image compression format and video compression format. As a result of the experiment, the proposed method shows 14.29% gain in BD-rate of BPP and mAP compared to the feature compression anchor of MPEG-VCM.

• Korean Journal of Ichthyology
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• v.33 no.4
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• pp.217-225
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• 2021

The family Platycephalidae is a taxonomic group of economically important demersal flathead fishes that predominantly occupy tropical or temperate estuaries and coastal environments of the Indo-Pacific oceans and the Mediterranean Sea. In this study, we for the first time analyzed the complete mitochondrial genome (mitogenome) of the flathead Platycephalus cultellatus Richardson, 1846 from Vietnam by Next Generation Sequencing method. Its mitogenome was 16,641 bp in total length, comprising 13 protein-coding genes (PCGs), two ribosomal RNA genes, and 22 transfer RNA genes. The gene composition and order of the mitogenome were identical to those of typical vertebrates. The phylogenetic trees were reconstructed based on the concatenated nucleotide sequence matrix of 13 PCGs and the partial sequence of a DNA barcoding marker, cox1 in order to determine its molecular phylogenetic position among the order Scorpaeniformes. The phylogenetic result revealed that P. cultellatus formed a monophyletic group with species belonging to the same family and consistently clustered with one nominal species, P. indicus, and two Platycephalus sp. specimens. Besides, the cox1 tree confirmed the taxonomic validity of our specimen by forming a monophyletic clade with its conspecific specimens. The mitogenome of P. cultellatus analyzed in this study will contribute valuable information for further study on taxonomy and phylogeny of flatheads.

• Journal of The Korean Association For Science Education
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• v.42 no.2
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• pp.289-309
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• 2022

In recent years, astronomy has been snowballing: including Higgs particle discovery, black hole imaging, extraterrestrial exploration, and deep space observation. Students are also largely interested in astronomy. The purpose of this study is to discover what needs to be improved in the current astronomy curriculum in light of recent scientists' researches and discoveries. We collected keywords from all papers published from 2011 to 2020 in four selected journals-ApJ, ApJL, A&A, and MNRAS- by R package to examine research trends. The curriculum contents were extracted by synthesizing the in-service teachers' coding results in the 2015 revised curriculum document of six subjects (Science, Integrated Science, Earth Science I, Earth Science II, Physics II, Convergence Science). The research results are as follows: first, keywords that appear steadily in astronomy are 'galaxies: formation, galaxy: active, star: formation, accretion, method: numerical.' Second, astronomy curriculum includes all areas except the 'High Energy Astrophysical Phenomena' area within the common science curriculum learned by all students. Third, it is necessary to review the placement of content elements by subject and grade and to consider introducing new concepts based on astronomy research keywords. This is an exploratory study to compare curriculum and the field of scientific research that forms the basis of the subject. We expect to provide implications for a future revision of the astronomy curriculum as a primary ground investigation.