• KSBB Journal
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• v.26 no.5
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• pp.427-432
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• 2011

In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3-glucanase was successfully expressed and secreted into the medium and the β--1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3-glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.

• The KIPS Transactions:PartD
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• v.15D no.1
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• pp.99-106
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• 2008

Recently developed compilers perform some optimizations in order to speed up the execution time of source program. These optimizations require the reordering of the sequence of program statements. This reordering does not give any problems in a single-threaded program. However, the reordering gives some significant errors in a multi-threaded program. State-of-the-art model checkers such as JavaPathfinder do not consider the reordering resulted in the optimization step in a compiler since they just consider a single memory model. In this paper, we develop a new verification tool to verify Java source program based on Java Memory Model. And our tool is capable of handling the reordering in verifying Java programs. As a result, our tool finds an error in the test program which is not revealed with the traditional model checker JavaPathFinder.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.05a
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• pp.21-25
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• 2002

Activated sludge processes are widely used in biological wastewater treatment processes. The main motivation of this research is to develop an intelligent control strategy for activated sludge process (ASP). ASP is a complex and nonlinear dynamic system because of the characteristic of wastewater, the change in influent rate, weather conditions, and so on. The mathematical model of ASP also includes uncertainties which are ignored or not considered by process engineer or controller designer. The ASP model based on Matlab/Simulink is designed in this paper. The performance of the model is tested by IWA (International Water Association) and COST (European Cooperation in the filed of Scientific and Technical Research) data that include steady-state results during 14 days. In this paper, fuzzy logic control approach is applied to control the DO (dissolved oxygen) concentration. The fuzzy logic controller that includes two inputs and one output can adjust air flowrate. Also, this paper introduces the remote monitoring and control system that is applied for the CSTR (Continuously Stirred Tank Reactor) wastewater treatment system. The CSTR plant has a local control and the remote monitoring system which is contained communication parts which consist of LAN (Local Area Network) network and CDMA (Code Division Multiple Access) wireless module. Remote control and monitoring systems are constructed in the laboratory.

• Nuclear Engineering and Technology
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• v.50 no.2
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• pp.229-236
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• 2018

Accident-tolerant fuels (ATFs) are currently of high interest to researchers in the nuclear industry and in governmental and international organizations. One widely studied accident-tolerant fuel concept is multilayer cladding (also known as coated cladding). This concept is based on a traditional Zr-based alloy (Zircaloy-4, M5, E110, ZIRLO etc.) serving as a substrate. Different protective materials are applied to the substrate surface by various techniques, thus enhancing the accident tolerance of the fuel. This study focuses on the results of testing of Zircaloy-4 coated with pure chromium metal using the cold spray (CS) technique. In comparison with other deposition methods, e.g., Physical vapor deposition (PVD), laser coating, or Chemical vapor deposition techniques (CVD), the CS technique is more cost efficient due to lower energy consumption and high deposition rates, making it more suitable for industry-scale production. The Cr-coated samples were tested at different conditions ($500^{\circ}C$ steam, $1200^{\circ}C$ steam, and Pressurized water reactor (PWR) pressurization test) and were precharacterized and postcharacterized by various techniques, such as scanning electron microscopy, Energy-dispersive X-ray spectroscopy (EDX), or nanoindentation; results are discussed. Results of the steady-state fuel performance simulations using the Bison code predicted the concept's feasibility. It is concluded that CS Cr coating has high potential benefits but requires further optimization and out-of-pile and in-pile testing.

• Tunnel and Underground Space
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• v.22 no.4
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• pp.257-265
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• 2012

In this study, a series of CFD (Computational Fluid Dynamics) simulations carried out to evaluate the optimum design model of the internal flow path of drill bit. The Star-CCM+ code was adopted to simulate the multi-phase discharge flow of rock particles and flushing air during a drilling process. The input parameters for the flow simulation of rock particles and air were obtained from the in-situ drilling test results. After the three design factors were determined, the experimental design method (Taguchi method) was utilized to evaluate the optimum value of each factor.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.637-640
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• 2005

The mechanical properties of bellows, such as the extensibility and the strength can be changed depending on the shape. For the shipbuilding material, it is favorable that the fatigue lift is long due to the elastic property and the reduction of thermal stress in piping system. Nowadays, the domestic production and design of bellows are based on the E.J.M.A Code. Therefore, the design standard is in need because of much errors and lack of detailed analysis. In this study, it is attempted to find out the optimal shape of U-type ship's bellows that is applied to design of experiment using the finite element method. The effective factors, mountain height, length, thickness, and number of mountains and the length of joint are considered and the proper values are chosen for the simulation. The number of mountains are increased, the volume increases above the standard volume and the stress obviously increases. In addition, the effect of the thickness of bellows on the stress is very large. Both of the volume and stress are decreasing at a certain lower value region.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.9
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• pp.2369-2378
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• 2009

In this paper, we implement measurement functionality for performance measurement of the GSM (Global System for Mobile Communication) terminal by using software. Generally speaking, the receiving algorithms in normal modems cannot be used directly to the measurement system due to the lack of the algorithm accuracy. In this paper, we propose the new receiver algorithm for precise GSM signal measurements. In the receiving algorithm, 2-stage (coarse stage, fine stage) parameters estimation (symbol-timing, frequency offset, carrier phase) scheme is used. To improve the estimation accuracy, we increase the number of the received signal samples by interpolation. The proposed GSM signal measurement algorithm can be used for verifying the hardware measurement system. In addition, the proposed algorithm can be used for the commercial system through code execution speed optimization.

• Journal of the Korean Society of Safety
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• v.33 no.6
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• pp.144-156
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• 2018

Level 3 Probabilistic Safety Assessment (PSA) is performed for the risk assessment that calculates radioactive material dispersion to the environment. This risk assessment is performed with a tool of MELCOR Accident Consequence Code System (MACCS2 or WinMACCS). For the off-site consequence analysis of multi-unit nuclear power plant (NPP) accident, the single location (Center Of Mass, COM) method has been usually adopted with the assumption that all the NPPs in the nuclear site are located at the same COM point. It was well known that this COM calculation can lead to underestimated or overestimated radionuclide concentration. In order to overcome this underestimation or overestimation of radionuclide concentrations in the COM method, Multiple Location (ML) method was developed in this study. The radionuclide concentrations for the individual NPPs are separately calculated, and they are summed at every location in the nuclear site by the post-processing of radionuclide concentrations that is based on two-dimensional Gaussian Plume equations. In order to demonstrate the efficiency of the ML method, radionuclide concentrations were calculated for the six-unit NPP site, radionuclide concentrations of the ML method were compared with those by COM method. This comparison was performed for conditions of constant weather, yearly weather in Korea, and four seasons, and the results were discussed. This new ML method (1) improves accuracy of radionuclide concentrations when multi-unit NPP accident occurs, (2) calculates realistic atmospheric dispersion of radionuclides under various weather conditions, and finally (3) supports off-site emergency plan optimization. It is recommended that this new method be applied to the risk assessment of multi-unit NPP accident. This new method drastically improves the accuracy of radionuclide concentrations at the locations adjacent to or very close to NPPs. This ML method has a great strength over the COM method when people live near nuclear site, since it provides accurate radionuclide concentrations or radiation doses.

• Journal of Korean Tunnelling and Underground Space Association
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• v.23 no.6
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• pp.423-438
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• 2021

The blade of motor grader is used for scattering and leveling the aggregates on the foundation of road construction site. The paper performed a design improvement research of the blade part to enhance the working efficiency of motor graders. The scattering works of aggregates by blade driving were simulated by DEM (discrete element method) of a dynamic code. The four design parameters were selected and a specific leveling scenario for the simulation was determined. The nine blade models were numerically experimented, and the sensitivity of each factors was analyzed. Next, the design factors that influence a blade performance have been selected by ANOVA, and these key design factors were applied to the progressive quadratic response surface method (PQRSM). The optimum set of design factors of the blade was finally proposed.

• Nuclear Engineering and Technology
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• v.53 no.7
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• pp.2184-2194
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• 2021

The Free-Piston Stirling engine (FPSE) is of interest for many research in aerospace due to its advantages of long operating life, higher efficiency, and zero maintenance. In this study, a 1-kW FPSE was proposed by analyzing the requirements of Space Reactor Power Systems (SRPS), of which performance was evaluated by developing a code through the Simple Analysis Method. The results of SAM showed that the critical parameters of FPSE could satisfy the designed requirements. The heater of the FPSE was designed with the copper rectangular fins to enhance heat transfer, and the parametric study of the heater was performed with Computational Fluid Dynamics (CFD) software STAR-CCM⁺. The Performance Evaluation Criteria (PEC) was used to evaluate the heat transfer enhancement of the fins in the heater. The numerical results of the CFD program showed that pressure drop and Nusselt number ratio had a linear growth with the height of fins, and PEC number decreased as the height of fins increased, and the optimum height of the fin was set as 4 mm according to the minimum heat exchange surface area. This paper can provide theoretical supports for the design and numerical analysis of an FPSE for SRPSs.