• Maxillofacial Plastic and Reconstructive Surgery
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• 제40권
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• pp.10.1-10.6
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• 2018

Background: The objective of this study was to evaluate the changes in gene expression after incubation of cells with proteins released from different silk mat layers. Methods: A silk cocoon from Bombyx mori was separated into four layers of equal thickness. The layers were numbered from 1 to 4 (from the inner to the outer layer). The proteins were released by sonication of a silk mat layer in normal saline. The concentration of proteins was determined by spectrophotometry. They were incubated with RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Results: Layer 1 and 4 groups had higher protein concentrations compared to those in layer 2 and 3 groups. The genes associated with inflammation and angiogenesis showed significantly higher expression in layer 1 and 4 groups. The results of qRT-PCR were in agreement with those of the cDNA microarray analysis. Conclusions: The silk mat from the middle portion of the silkworm cocoon yielded a lower protein release and caused an insignificant change in the expression of genes that are associated with inflammation and angiogenesis.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 1997년도 Progress and Future Development of Sericultural Science and Technology 40th Anniversary Commemoration Symposium
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• pp.1-21
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• 1997

Silk is praised "Queen of Fiber", which wrote a glorious page in the history of human adornment culture. Thanks to the development of science and technology, silk production has been increased dramatically during last half century. Nowadays, as "Healthy Fiber" silk lets all the globe to be forming the "Silk Boom" Like in other scientific fields, a great deal of progress has been made in sericultural science and technology, which also accelerated the development of multipurpose utilization of sercultural recourses. The main tendency of the sericulture development is raising the quality of cocoon, and at the same time, increasing the output per unit mulberry area and labor production efficiency. The chief measure of raising the cocoon quality is breeding and selecting the new mulberry and silkworm varieties, preventing and curing the diseases and pests and improving the environment of mounting, and the symble for increasing the labor productivity is carrying out the technological system of saving labor and operation ease, and enlarging the production scale. As a natural protein fiber, silk will be still a favorite with human in the next century, because its structure and properties can not be replaced by other any fiber. The 21st century is the biological time, and measures of developing sericulture will use the achievement on the field of molecular biology and biotechnology to carry out the molecular breeding, development regulating, silkworm rearing industrializing, exploiting the new silk and its new usage, utilizing the sericultural resources, and producing the special protein from silkworm as a host of expressing exogenous genes so that make the sericulture to contribute more what it can do for the human being.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 공동 춘계학술대회
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• pp.69.2-69
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• 2000

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.135.1-135
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• 1996

No Abstract, See Full Text

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.87-90
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• 2000

This paper has reviewed the China Sericulture history in the 20th century. On the basis of the current sericulture production, sericulture area distribution, problems and challenges, it has been forecasted that China sericulture will be developed as following points: 1) On a stable basis, cocoon production increased gradually to a suitable amount; 2) Quality cocoon production bases established to form a regionalized, industrialized and specialized sericulture structure; 3) With increase of householding rearing capacities, rearing facilities improved; 4) Production efficiency raised and silk product quality improved through technical innovation; 5) New and diversified products developed from Sericultural resources with new and high technologies.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권1호
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• pp.13-19
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• 2005

Biotechnological researches in Central Asian Research Institute of Sericulture were developed since 1981 when laboratory of artificial diets for mulberry silk­worm was created. The researches were directed not only to creation of accessible artificial diets (AD) for off-season mass rearing of economical beneficial insect and for industrialization of sericulture but also to obtaining of easy reproducible source of animal proteins, carbohydrates, lipids, enzymes, enzymes inhibitors and other metabolites - bioresource for manufacture of oriental medicine preparations. Space experiments with silkworms became possible with use of AD. These experiments used mulberry silkworm as high effective board test organism with outstanding developmental, reproductive and transgenic properties having impor­tant significance in future of cosmonautics. Introduction to practice of bioprotective and ecologically pure method of drying and sterilizing of green cocoons and by-products of silk manufacture allows to increase efficiency of cocoons reeling technology as well as to preserve nativity of biological active substances in mulberry silkworm pupae that is unachievable by traditional methods of raw material processing. New spheres of application of two important silk proteins - fibroin and sericin in medicine, cosmetics, for bio- and nanotechnology are opening with use of biotechnological approaches. At present the achievements and experience of R & D in these fields are used in creation of modern pests biocontrol agents. Schemes of efficient processing of cocoon raw material, by-products and wastes of sericulture and cocoon processing are devel- Institute of Physiology and Biophysics, Uzbek Academy of Sciences, I, Niyazov street, 700095, Tashkent, Uzbekistan. oped for obtaining of traditional and new consumer products.

• 한국잠사곤충학회지
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• 제49권1호
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• pp.14-17
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• 2007

다수성 품종으로 육성된 골든실크는 새로운 봄누에 특수용도 품종으로서 육성경위와 주요특성을 하면 다음과 같다. 1. 일본종계 원종 잠311은(견색적$\times$흑아)$\times$잠125 교잡 육성한 무늬 품종으로 황색고치를 짓고, 중국종계 원종 잠312는 잠304$\times$괴축과 교잡 육성한 무늬 없는 누에품종으로 황색고치를 짓는 품종이다. 2. 2006년 춘잠기 지역적응시험 결과는 골든실크의 주요특성은 - 실용 부화비율은 97%, 화용비율 및 상견비율은 대조품종보다 우수하였다. 수량성 등 다른 조사항목에서는 대조보다 좋지 못하였으나, 전령기간은 1일 12시간이 짧아 사육노동력을 줄일 수 있는 이점이 있다. - 견사장은 1,031m였고, 견사섬도는 2.97 데니어 이었다. - DNJ 함량은 대조 춘수잠 3.81 mg보다 14% 높은 4.34mg으로 우수 하였고, 동충하초 생산력 검정에서는 동충하초 생체중이 1개당 대조 1.41g보다 낮은 1.20g이였다. 3. 잠종생산능력에 있어서는 일본종계 원종 잠311의 나방당 산란수는 604개로 대조보다 많았고, 최청에서 발아까지의 일수는 54일 이었다. 중국종계 원종 잠312의 산란수는 493개로 일반장려품종의 원종과 산란성이 비슷하였고, 최청에서 발아까지의 일수는 52일이었다.

• 한국잠사곤충학회지
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• 제15권1호
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• pp.27-30
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• 1973

본 연구는 조사검정의 정도를 높이고 또한 조사검정을 보다 효율적으로 수행하는데 기여하기 위한 기초자료를 얻고자 하였던바 다음과 같은 결과를 얻었다. 1. 검정시료량에 대한 해서율 및 생사량비율의 표준편차와 변이계수는 시료량의 증가에 따라서 점차감소하는 경향을 보였는데 300입이상의 시험구에서는 시료량의 다소에 따른 유의차를 인정할 수 없었다. 2. 해서율에 대한 표준오차는 300 입구와 400 입구간의 유의차를 인정할 수 없었다. 3. 생사량비율에 대한 표준오차는 300 입구와 500 입구 및 600 입구간의 유의차를 인정할 수 없었다. 4. 견사장에 대한 표준편차, 표준오차 및 변이계수는 시료량의 다소에 따론 유의차를 인정할 수 없었다. 5. 이상의 연구결과를 종합하여 볼때 견사검정에 있어서의 적정시료량은 300입으로 추정된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권2호
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• pp.83-88
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• 2017

Silk is appealing materials for many biomedical applications involving tissue engineering and implantable devices, because of its biocompatibility, environmental stability, controlled proteolytic biodegradability and morphologic flexibility. Silk matrix is required for the treatment of a wide wound area, but the present silk matrix is made by the second processing, and thus, the labor and the cost are high. In this work, we investigated the optimal production condition of natural silk 3D matrix using the silkworms and invented Automatic Silk Matrix Making Machine (ASMMM) for natural silk 3D matrix production. As a result, we determined that optimal production condition for making A4 paper size natural silk 3D matrix was used Rough aquarelle paper on surface at $25^{\circ}C$ and 30 silkworm larvae. These results are expected to provide basic data for the efficient production of the natural silk 3D matrix, and it is suggested that the produced natural silk 3D matrix is useful as a medical biomaterials.

• 한국잠사곤충학회지
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• 제53권2호
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• pp.92-96
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• 2015

본 연구는 장려 품종 누에고치로부터 실크 세리신과 실크 피브로인으로 분리하여 이들 단백질을 세포 첨가제로 개발하기 위하여 수행되었다. 장려품종 누에고치를 고온 고압기를 통하여 실크 세리신을 먼저 추출하였으며, 남겨진 실크 피브로인을 이용하여 시간별로 용해하여 품종별 실크 피브로인을 얻었다. 이들 실크 세리신과 실크 피브로인을 이용하여 세포 독성, 세포 증식 및 분열 관련 유전자 발현 분석을 확인하였다. 먼저, 누에고치의 특성을 분석하기 위하여 공극률을 측정하였다. 공극률은 금옥잠 누에고치의 경우 84.71%, 대성잠 81.58%, 백옥잠 73.23%의 공극률 값이 나타났다. 분자량 차이에 있어서 실크 세리신은 품종의 영향이 크지 않았으나 실크 피브로인의 경우, 금옥잠은 5시간의 장시간의 용해에도 불구하고 100 kDa 이상의 큰 분자량을 나타내었다. 장려품종 누에를 사용하여 제조된 실크 세리신과 실크 피브로인을 이용하여 세포 증식 실험을 하였으며 백옥잠으로부터 얻은 실크 세리신의 경우 농도에 따라 유의적으로 세포 증식 효과가 나타났으며, 금옥잠으로부터 얻은 실크 피브로인의 경우 5시간 용해 시 세포 증식에 있어서 가장 우수한 결과를 보였다. 또한, 백옥잠 실크 세리신 처리 결과 세포 증식 관련 유전자의 발현수준이 증가됨을 확인할 수 있었다.