• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.717-726
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• 2017

The current allowable cross-contamination level of fatty acid methyl esters (FAME) in aviation turbine fuel (AVTUR) is 50 mg/kg, due to that the presence of FAME in AVTUR can significantly impact the fuel supply system and jet engine. It has been difficult to analyze the level of FAME in AVTUR, since it is consisted of a lot of hydrocarbons. In this study, thus, a new method using multi-dimensional GC-MS (MDGC-MS) was proposed in order to determine the FAME level in AVTUR effectively. Applying to MDGC-MS with Deans switching system enabled us to detect and quantify the FAME with low carbon numbers such as those derived from coconut oil and palm kernel oil. The matrix effect of MDGC-MS method, which could shift the FAME peaks to slightly longer retention times, was reduced by 20 times compared with that of 1-dimensional GC-MS reference method. This developed method could be suitable for qualitative and quantitative analyses to determine the contamination level of trace FAME in AVTUR.

• Journal of Applied Biological Chemistry
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• v.53 no.2
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• pp.65-70
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• 2010

Medium-chain fatty acids (MCFA) are composed of 8-12 carbon atoms, and are found in coconut, cuphea, and palm kernel oil. MCFA were introduced into clinical nutrition in the 1950s for dietary treatment of malabsorption syndromes because of their rapid absorption and solubility. Recently, MCFA have been applied to Gastrointestinal Permeation Enhancement Technology (GIPET), which is one of the most important parts in drug delivery system in therapeutics. Therefore, to accumulate the MCFA in seed oil of rapeseed, much effort has been conducted by classical or molecular breeding. Laurate can be successfully accumulated up to 60 mol% in the seed oil of rapeseed by the expression of bay thioesterase (Uc FatB1) alone or crossed with a line over-expressing the coconut lysophosphatidic acid acyltransferase (LPAAT) under the control of a napin seed-storage protein promoter. Also, caprylate and caprate were obtained 7 mol% and 29 mol%, respectively, from plants over-expressing of the medium-chain specific thioesterase (Ch FatB2) alone or together with the chain-length-specific condensing enzyme (Ch KASIV). Despite the success of some research in utilizing parallel classical and molecular breeding to produce MCFA, commercially available seed oils have for the most part, not been realized. Recent research in the field of developing MCFA-enriched transgenic plants has established that there is no single rate-limiting step in the production of the target fatty acids. The purpose of this article is to review some of the recent progress in understanding the mechanism and regulation of MCFA production in seed oil of rapeseed.