• Journal of the Korean Society of Marine Environment & Safety
• /
• v.18 no.5
• /
• pp.398-405
• /
• 2012

PAHs(Polycyclic Aromatic Hydrocarbons) in coastal area and estuary adjacent to urban area and industrial activities region are mainly introduced into marine environment via atmosphere and river. This study which is evaluated the distribution characteristics of PAHs discharge from rivers and sewage disposal water which flowing into Ulsan bay, and were carried out in Jun of dry season and in Auguest of wet season, 2008. The water samples from eight main rivers and Youngyeon WWTP(Wastewater Treatment Plant) flowed into Ulsan bay were taken for analysis of dissolved PAHs. The range and mean values of dissolved PAHs concentrations showed 10.30 to 87.88 ng/L, its mean 36.94 ng/L in dry season and 10.30 to 69.57 ng/L, its mean 24.37 ng/L in wet season. The distribution of dissolved PAHs showd the high concentrations in the Gungcheoncheon which is flowed from urban and industrial area. The ranges and means values of the loading fluxes were estimated with 0.04 to 8.27 g/day, its mean 2.05 g/day in dry season, and 0.03 to 4.77 g/day, its mean 1.61 g/day, in wet season. The loading flux showed the highest value in Taewha river due to the high flow rate and the urban activity. The composition patterns of dissolved PAHs compound showed most of the trend occupying low molecuar weight PAHs than high molecular weight PAHs. These results were due to physical and chemical characteristics of PAHs compound, and were similar to those of other studies. The concentrations of dissolved PAHs in this study are lower than those of other studies, and the level of PAHs pollution appeared to be not serious in reverine waters of Ulsan coast.

• Korean Journal of Plant Resources
• /
• v.22 no.2
• /
• pp.116-122
• /
• 2009

Heteropappus arenarius Kitam., an autumn-flowering biennial belonging to wild chrysanthemums, is found to be native in southeastern coastal area and Jeju island of Korea. It could play a good role for ground cover plants on a large-scale landscape area, especially, barren soil or sloping hillside. This study was initiated to screen optimum germination temperature influenced by local strain and harvesting stage of H. arenarius. The following was the response of seed germination between local strain and temperature. The average of final germination percentage (FG) was the highest in 'Guryongpo' (89.7%) among four local strains, followed by 'Gujwa' (87.3%), 'Gampo' (87.3%), and 'HKNU-I' (71.5%). The average of $T_{50}$ was shorter in 'Gujwa' (3.6 d) and 'Guryongpo' (4.0 d) than the others. The average of FG and $T_{50}$ was the highest as 76.2% and shortest as 3.6 d in $20^{\circ}C$, respectively, followed by $30^{\circ}C$, $25^{\circ}C$, and $15^{\circ}C$. In case of 'Gujwa', however, FG and T50 was higher in $20^{\circ}C$ and shorter in $15^{\circ}C$ than others. In the relationship between harvesting stage and temperature, the average of FG was greatly higher in Stage III (90.7%) and Stage IV (88.6%) than the others including Stage II (35.7%) and Stage I (26.0%). The average of $T_{50}$ was shorter in Stage IV (3.7 d) and Stage III (4.3 d) than the others, which showed less than 50% of FG. Nevertheless, the available range of seed harvesting stage was from Stage I to Stage IV because H. arenarius seeds could germinate at all stages. In conclusion, it was recommended that the optimum temperature and harvesting stage was $20^{\circ}C$ and Stage $III{\sim}IV$, respectively, for seed germination of H. arenarius.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.3
• /
• pp.341-348
• /
• 2010

To investigate the effect of continual pre-plant application of liquid pig manure (LPM) on malting barley growth, quality and soil environment in double cropping system of rice and malting barley, the liquid pig manure was applied after harvesting rice and malting barley for 3 years. Field experiment was designed with non-fertilizer, chemical fertilizer (CF) recommended by soil testing, rice (LPM 50%+CF 50%)+malting barley (CF 100%), rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%), rice (LPM 100%)+malting barley (CF 100%) and rice (LPM 100%)+malting barley (LPM 100%). By continuous application of LPM 100%, the contents of available $P_2O_5$ and exchangeable K in the soil were increased. The available $P_2O_5$ increased from 243 to 350 mg $kg^{-1}$ and exchangeable K was changed 0.31 to 0.44 $cmol_{c}\;kg^{-1}$. However, the contents of available $P_2O_5$ and exchangeable K were not significant changes in rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%) plot. Bulk density of soil was not affected by application of LPM. The microbial density was high in order of bacteria > actinomycetes > fungi. The population of aerobic bacteria in rice (LPM 100%)+malting barley (LPM 100%) plot was higher than other plots. The ratio of aerobic bacteria/fungi and biomass C content were the highest in rice (LPM 100%)+malting barley (LPM 100%) plot. The yield of malting barley was increased 22% by increasing culm length, panicle length, No. of panicle and 1,000 grains as 358 kg $10a^{-1}$ in rice (LPM 100%)+malting barley (LPM 100%) plot compared with 294 kg $10a^{-1}$ in rice (CF 100%)+malting barley (CF 100%) plot. The content of ${\beta}$-glucan was low by 4.5 and 4.4% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot, respectively. The content of crude protein was the lowest by 8.2% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot and the quality of malting barley was good as within 11%.

• Korean Journal of Environment and Ecology
• /
• v.30 no.6
• /
• pp.939-961
• /
• 2016

For effective conservation of endangered wild plants, it is necessary to understand their interactions with environmental factors in each habitat together with life traits of target species. First, the characteristics of their distribution were investigated followed by their monitoring for 4 years focusing on the habitats in the lagoon. Also, their life traits were compared including production of hibernacles, fruits, and seeds by the soil fertilization and light intensities. Next, the information on the species was secured by germination experiment using the generated seeds from the cultivation experiment. The habitat of Glaux maritima var. obtusifolia Fernald in Korea was located in the rear edge of the worldwide distribution and its four habitats were isolated and distributed far away each other. Two of them were located in small salt-marsh and fine sand estuaries formed in the rocky area of the seashore, and the other two were inhabited with the sandy soil in the lagoon which was connected by river-mouth to the sea. Glaux maritima var. obtusifolia Fernald tends to be distributed in the sites where the establishment and growth of the competitor were inhibited by salinity, periodic flooding, and lower layer of the soil to extend a roots. It maintained its population by recruitments of hibernacles and seedling. The production of hibernacle was assumed to be affected by the particle consist of the sand together with organic matters in the soil. Seedling recruitment was observed only in the salt-marsh area located in the rear sites of sand ridge where was the shore of the lagoon. Glaux maritima var. obtusifolia Fernald was observed to have different threatening factors by each population. Its population in Pohang seemed the sedimentation of fine sand which affected the recruitment of hibernacles had been eroded due to the construction of the coastal road. The population in Ulsan appeared rapid expansion of competitor and reduction of its distribution area due to the interruption of eluted water supplied to the habitat. On the other hand, the habitat in the lagoon maintained the population relatively stable. Especially, the population in Songji-ho was determined to be the most stable one. To sustain the population of Glaux maritima var. obtusifolia Fernald distributed in the lagoon, it is suggested that the wide ranged scale of conservational activities is necessary to maintain the mechanisms including the entrance of seawater which belongs to the lagoon, and periodic flooding.

• Horticultural Science & Technology
• /
• v.33 no.6
• /
• pp.891-899
• /
• 2015

The lily symptomless virus (LSV) is the most common virus in Korean native lilies and causes various types of damage to overall plant growth. This study was carried out to investigate the elimination rate of the LSV by the in vitro scale culture (scaling) method in Korean native lilies and to test reinfection rates of the LSV under several field culture conditions of bulb production. Four Korean native lilies (Lilium dauricum, L. distichum, L. lancifolium, and L. maximowitzii) were used and their scales were cultured in vitro for micro-scale formation. The micro-scales were subcultured repeatedly using MS culture medium supplemented with 30 or $90g{\cdot}L^{-1}$ sucrose. The culture conditions were $24{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with 16 hour daylength using fluorescent lamps and maintained at $22{\pm}2^{\circ}C$. The virus-free bulblets were grown for one to three years in the greenhouse and transplanted to the field in October or March. Virus infection rates were investigated by direct tissue blotting immunobinding assays and measurement of chlorophyll and protein contents. Virus-free plants could be obtained from the 5th subculture of micro-scales in L. lancifolium and L. maximowitzii or from primary culture in L. dauricum and L. distichum. LSV-free plants were reinfected during bulb production in the field. Reinfection rates were higher at older bulb ages and under higher planting density. The plants planted in October and at inland Gyeongsan had higher infection rates than those planted in March and at coastal area Pohang. The reinfection rate of L. maximowitzii was higher than those of L. dauricum and L. lancifolium. The LSV-infected plants had lower chlorophyll contents and unchanged protein contents compared to virus-free plants.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.15 no.4
• /
• pp.275-282
• /
• 2009

PAHs(Polycyclic Aromatic Hydrocarbons) compounds may enter into the marine environment in a number of ways, and PAHs in coastal area and estuary adjacent to urban area and industrial activities region are mainly introduced into marine environment via atmosphere or river. The field surveys to evaluate the pollution of PAHs in rivers of Jinhae bay were carried out in August, 2008. The water samples collected from seven main rivers and Deokdong WWTP(Waste water Treatment Plant) which lead to Jinhae bay. 16 PAHs which had been selected as priority pollutants by EPA were analyzed. The range and mean of dissolved Total PAHs concentrations in the rivers and Duckdong WWTP that lead to Jinhae bay were shown to be 9.79~128.25(mean 36.94)ng/L, while the range and mean of Total PAHs concentrations in SPM(Suspended Particulate Matter) were shown to be 1,81434~8,893.37(mean 4,657.73)${\mu}g$/kg dry wt. The dissolved Total PAHs and Total PAHs in SPM were shown to be high concentrations in the Semhocheon which leads to Masan bay that is the most polluted area of Jinhae bay, while those were shown to be low concentrations at rivers which lead to the west area of the bay. The ranges and means of the loading fluxes from rivers and Duckdong WWTP were calculated at 0.06~12.05(mean 1.86)g/day for dissolved Total PAHs and at 0.12~16.00(mean 2.41)g/day tor Total PAH in SPM. The loading flux of Total PAHs from Duckdong WWTP was shown to be the greatest, occupying more than 80% of Total loading flux from all rivers and WWTP to Jinhae bay. The composition patterns of PAHs compound by the number of benzene ring between dissolved PAHs and PAHs of SPM were different each other. Low molecuar weight PAHs of dissoloved compounds were in high concentration, while high molecular weight PAHs of SPM were in high concentration. These results were due to physical and chemical characteristics of PAHs and were similar to those of other studies. The total PAHs concentrations of dissolved and SPM in this study are lower than those of other studies. The extent of PAHs pollution appeared to be not serious in reverine waters of Jinhae bay.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.35 no.4
• /
• pp.352-361
• /
• 1990

The reducing effect of wind injury was investigated using several wind-break nets in Youngdeok province where cold-wind damage is often occurred during rice growing season. The white-head damage of rice have been often occurred by typhoon during the period between August 15 to September 10 in the cold wind area of the eastern coastal during the last 11 years (1979-1989). This may suggest that the critical period for heading will be by August 15 in the regions. High evaporation coefficient, more than 250 due to typhoon passage over the regions resulted high injury of white head. Generally, the wind injury have been caused by warm and dry westerlies through Fohn apperance in Taebaeg mountains and by cool-humid wind which blows from coast to inland. The frequency of occurrence of the two types of typhoons were 25, 20%, respectively during rice cultivation. The instalation of wind-break net significantly reduced the wind blowing speed, depending on the net mesher with the higher effect in dence net. The distances between the net and cropping area also affect the wind speed: 23% reduction at 1m distance. 34% at 10m and 28% at 20m, respectively. The reducing effect was also observed even at 10 times height of the wind-break net. The instalation of wind-break net gave several effects on climate factor, showing that temperature increased by 0.8$^{\circ}C$(maximum), 0.7$^{\circ}C$(minimum), 0.6$^{\circ}C$(average) : water temperatures increased by 0.5$^{\circ}C$(maximum), 0.6$^{\circ}C$(minimum), 0.5$^{\circ}C$(average) : soil temperature increased 0.4$^{\circ}C$. The earlier heading and increasing growth rate, use of light, culm length, panicle number per hill, spikelet number per panicle, fertility and 1,000 grain weight were observed in the fields with the wind-break nets resulting in 10-15% increase in rice yield using 0.5${\times}$0.5cm nets. The increasing seedlings per hill gave higher grain yield by 13% in the cold wind damage regions of eastern coastals. and the wind-break was more significant in the field without the wind-break net. Wind injury of rice plant in the cold wind regions of eastern coastals in korea could be reduced by selection of tolerant varieties to wind injury, adjustment of transplanting time, and establishment of wind-break nets.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.5
• /
• pp.631-636
• /
• 2010

One experiment was carried out to investigate the soil chemical properties of bracken growth and the inorganic element contents of plant. To the results of soil analysis in native bracken (Pteridium aquilinum L.) growth, soil pH was 5.2, organic matter was 19 g $kg^{-1}$ and available phosphate was 20 mg $kg^{-1}$, and exchangeable potassium, calcium, magnesium were 0.32, 2.0 and 1.3 $cmol_c\;kg^{-1}$, respectively. In the bracken cultivation soil, pH was 5.7, organic matter was 13 g $kg^{-1}$ and available phosphate 367 mg $kg^{-1}$, and exchangeable potassium, calcium and magnesium were 0.81, 4.0 and 1.4 $cmol_c\;kg^{-1}$, respectively. The soil pH, available phosphate and exchangeable calcium were much lower in bracken native soil than those of cultivation soil, while organic matter was a little higher in native soil than that of cultivation soil. In native bracken plants, three major elements of nitrogen, phosphorus and potassium, were 4.40, 0.55 and 3.40%, calcium and magnesium were 0.22 and 0.32%, and microelements of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn) and boron were 126, 210, 23, 75 and 11 mg $kg^{-1}$, respectively. In cultivation bracken, three major elements of nitrogen, phosphorus and potassium, were 5.50, 0.73 and 3.55%, calcium and magnesium were 0.17 and 0.28%, and microelement contents of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn) and boron (B) were 120, 252, 19, 72 and 20 mg $kg^{-1}$, respectively.

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.4
• /
• pp.289-300
• /
• 2009

Flora distribution was surveyed in the newly reclaimed tidal flats in the west coastal area in Korea to understand changes in flora dynamics after reclamation. The surveyed reclaimed tidal flats were the newly reclaimed tidal flat in Hangdam, the mid Kyonggi Bay in 2002, and three reclaimed lands in Seukmun and Daeho, Chungnam, and Hangdam in Kyonggi Bay, of which reclamation years based on embankment were 7, 9 and 18 years, respectively. In the newly reclaimed tidal flat, the dominant flora was Suaeda japonica and other florae were rare, while various halophytes and glycophytes were distributed in the reclaimed lands. On the newly reclaimed tidal flat, four species of halophytic pioneer florae, Salicornia europaea, Suaeda glauca, Suaeda japonica, and Suaeda maritime occurred, and along with age facultative halophyte and glycophyte occurred sequently. On the reclaimed lands, the florae were more complex with various facultative halophyte and glycophyte, so these were predominated rather than pioneer halophyte, while one of pioneer halophyte that Suaeda japonica was not occurred. Increasing of various facultative halophyte and glycophyte, and decreasing of pioneer halophyte indicated that flora changed toward to increase of facultative halophyte and glycophyte by aged after reclamation. On the newly reclaimed tidal flat the ratio of flora species changed rapidly with the invasion of plant. This implied that the flora had begun to change in the early stage of reclamation. Facultative halophyte and glycophyte started to increase on the early stage of reclamation but relative density and frequency of pioneer halophyte was higher than facultative halophyte and glycophyte. According to the investigation up to 3 years after reclamation, pioneer halophyte predominated on it. Although flora changed, there were common representative halophytes among the reclaimed tidal flats: Salicornia europaea, Suaeda maritima, and Suaeda glauca as pioneer halophyte, Aster tripolium, Sonchus brachyotus, and Phragmites communis as facultative halophytes.

• Horticultural Science & Technology
• /
• v.35 no.3
• /
• pp.344-360
• /
• 2017

Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.