• Title/Summary/Keyword: Co-laboratory

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Synthesis and application of zirconium phosphate mesoporous coordination polymer for effective removal of Co(II) from aqueous solutions

  • Yang Zeng;Guoyuan Yuan;Tu Lan;Feize Li;Jijun Yang;Jiali Liao;Yuanyou Yang;Ning Liu
    • Nuclear Engineering and Technology
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    • v.54 no.11
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    • pp.4013-4021
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    • 2022
  • A kind of zirconium phosphate mesoporous coordination polymer Zr-EDTMPA was successfully synthesized and characterized using XRD, FTIR, TGA, EA, SEM-EDS, and N2 sorption-desorption measurements. The prepared Zr-EDTMPA was first employed for the removal of Co(II) from an aqueous solution, and the effects of pH, contact time, temperature, initial Co(II) concentration, reusability, and sorption mechanism were systematically investigated. The results showed that the Zr-EDTMPA is a zirconium phosphate complex formed by the coordination of EDTMPA to Zr in a molar ratio of 1:1. The sorption of Co(II) by Zr-EDTMPA was a pH-dependent, spontaneous and endothermic process, which was better fitted to the pseudo-second-order kinetic model and Langmuir isotherm model. The Zr-EDTMPA was demonstrated to have excellent reusability and presented a high sorption capacity of 73.0 mg·g-1 for Co(II) at pH 8.0. The sorption mechanism was mainly attributed to the strong coordination between cobalt and the untapped hydroxyl functional groups on Zr-EDTMPA, which was confirmed by XPS spectra. Therefore, as a candidate sorbent with high sorption capacity and excellent reusability, Zr-EDTMPA has a great potential for the removal of Co(II) from aqueous solutions.

A Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042

  • Sun, Yunlong;Niu, Yali;He, Bin;Ma, Long;Li, Ganghua;Tran, Van-Tuan;Zeng, Bin;Hu, Zhihong
    • Journal of Microbiology and Biotechnology
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    • v.29 no.2
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    • pp.230-234
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    • 2019
  • Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

Effects of Protease-resistant Antimicrobial Substances Produced by Lactic Acid Bacteria on Rumen Methanogenesis

  • Reina, Asa;Tanaka, A.;Uehara, A.;Shinzato, I.;Toride, Y.;Usui, N.;Hirakawa, K.;Takahashi, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.6
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    • pp.700-707
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    • 2010
  • Effects of protease-resistant antimicrobial substances (PRA) produced by Lactobacillus plantarum and Leuconostoc citreum on rumen methanogenesis were examined using the in vitro continuous methane quantification system. Four different strains of lactic acid bacteria, i) Lactococcus lactis ATCC19435 (Control, non-antibacterial substances), ii) Lactococcus lactis NCIMB702054 (Nisin-Z), iii) Lactobacillus plantarum TUA1490L (PRA-1), and iv) Leuconostoc citreum JCM9698 (PRA-2) were individually cultured in GYEKP medium. An 80 ml aliquot of each supernatant was inoculated into phosphate-buffered rumen fluid. PRA-1 remarkably decreased cumulative methane production, though propionate, butyrate and ammonia N decreased. For PRA-2, there were no effects on $CH_4$ and $CO_2$ production and fermentation characteristics in mixed rumen cultures. The results suggested that PRA-1 reduced the number of methanogens or inhibited utilization of hydrogen in rumen fermentation.

2D Coordination Polymer Derived Co3O4 Nanocrystals as High Performance Anode Material of Lithium-Ion Batteries

  • Wen, Hao;Shi, Changdong;Gao, Yuanrui;Rong, Hongren;Sha, Yanyong;Liu, Hongjiang;Liu, Qi
    • Nano
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    • v.13 no.12
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    • pp.1850139.1-1850139.10
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    • 2018
  • $Co_3O_4$ nanocrystals have been synthesized via an ordinary one-step calcination of a cobalt-based 2D coordination polymer [Co(tfbdc)(4,4'-bpy)$(H_2O)_2$]. As an anode material for lithium-ion batteries, the obtained $Co_3O_4$ nanocrystals exhibit high reversible capacity, excellent cyclic stability and better rate capability. The reversible capacity of the $Co_3O_4$ nanocrystals maintains $713mA\;h\;g^{-1}$ after 50 cycles at a current density of $50mA\;g^{-1}$. Our results confirm that searching for metal oxides nanomaterials used as anode materials of lithium ion batteries via the calcinations of 2D coordination polymer is a new route.

Lossless snubber for high power density modular power converter (고 전력밀도 모듈형 전력컨버터를 위한 무손실 스너버)

  • Shin, Jung-Min;Kim, Su-San;Park, Chul-Wan;Kweon, Gie-Hyoun;Heo, Tae-Won;Ryu, Dong-Kyun;Choi, Heung-Gyoon;Kim, Hugh;Han, Sang-Kyoo
    • Proceedings of the KIPE Conference
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    • 2015.07a
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    • pp.271-272
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    • 2015
  • 본 논문은 무손실 스너버 회로를 적용함으로써 스위치의 온/오프 시 발생하는 스위칭 손실을 저감시킬 뿐만 아니라 출력 다이오드의 역 회복 특성을 개선하여 보다 향상된 효율을 갖는 강압형 컨버터를 제안한다. 또한 스위칭 손실을 줄여 고 주파수 구동을 가능하게 하고, 이를 통해 전원회로의 고 전력밀도 구현을 목적으로 하고 있다. 제안된 무손실 스너버의 타당성 검증을 위해 이론적 분석과 실험결과를 제시한다.

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In Situ-DRIFTS Study of Rh Promoted CuCo/Al2O3 for Ethanol Synthesis via CO Hydrogenation

  • Li, Fang;Ma, Hongfang;Zhang, Haitao;Ying, Weiyong;Fang, Dingye
    • Bulletin of the Korean Chemical Society
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    • v.35 no.9
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    • pp.2726-2732
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    • 2014
  • The promoting effect of rhodium on the structure and activity of the supported Cu-Co based catalysts for CO hydrogenation was investigated in detail. The samples were characterized by DRIFTS, $N_2$-adsorption, XRD, $H_2$-TPR, $H_2$-TPD and XPS. The results indicated that the introduction of rhodium to Cu-Co catalysts resulted in modification of metal dispersion, reducibility and crystal structure. DRIFTS results of CO hydrogenation at reaction condition (P=2 MPa, $T=260^{\circ}C$) indicated the addition of 1 wt % rhodium improved hydrogenation ability of Cu-Co catalysts. The ethanol selectivity and CO conversion were both improved by 1 wt % Rh promoted Cu-Co based catalysts. The alcohol distribution over un-promoted and rhodium promoted Cu-Co based catalysts obeys A-S-F rule and higher chain growth probability was got on rhodium promoted catalyst.

Blood test results from simultaneous infection of other respiratory viruses in COVID-19 patients

  • In Soo, Rheem;Jung Min, Park;Seung Keun, Ham;Jae Kyung, Kim
    • International Journal of Advanced Culture Technology
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    • v.10 no.4
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    • pp.316-321
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    • 2022
  • Since 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly, infecting millions of people worldwide. On March 11, 2020, the World Health Organization declared coronavirus disease (COVID-19) a pandemic owing to the worldwide spread of SARS-CoV-2, which created an unprecedented burden on the global healthcare system. In this context, there are increasing concerns regarding co-infections with other respiratory viruses, such as the influenza virus. In this study, clinical data of patients infected with SARS-CoV-2 and other respiratory viruses were compared with patients infected with SARS-CoV-2 alone. The hematology and blood biochemistry results of 178 patients infected with SARS-CoV-2 , who were tested on admission, were retrospectively reviewed. In patients with SARS-CoV-2 and adenovirus co-infection, C-reactive protein levels were elevated on admission, whereas lactate dehydrogenase (LDH), prothrombin time, international normalized ratio, activated partial thromboplastin clotting time, and bilirubin values were all within the normal range. Moreover, patients with SARS-CoV-2 and human bocavirus co-infection had low LDH and high bilirubin levels on admission. These findings reveal the clinical features of respiratory virus and SARS-CoV-2 co-infections and support the development of appropriate approaches for treating patients with SARS-CoV-2 and other respiratory virus co-infections.

A Three-dimensional Magnetic Field Mapping System for Deflection Yoke of Cathode-Ray Tube

  • Park, K.H.;Yoon, M.;Kim, D.E.;Lee, S.M.;Joo, H.D.;Lee, S.D.;Yang, W.Y.
    • Journal of Information Display
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    • v.3 no.4
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    • pp.19-22
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    • 2002
  • In this paper, we introduce an efficient three-dimensional magnetic field mapping system for a Deflection Yoke (DY) in Cathode-Ray Tube (CRT). A three-axis Hall probe mounted in a small cylindrical bar and three-stepping motors placed in a non-magnetic frame were utilized for the mapping. Prior to the mapping starts, the inner contour of DY was measured by a laser sensor to make a look-up table for inner shape of DY. Three-axis magnetic fields are then digitized by a three-dimensional Hall probe. The results of the mapping can be transformed into various output formats such as multi pole harmonics of magnetic fields. Field shape in one, two and three- dimensional spaces can also be displayed. In this paper, we present the features of this mapping device and some analysis results.

The Progression of SARS Coronavirus 2 (SARS-CoV2): Mutation in the Receptor Binding Domain of Spike Gene

  • Sinae Kim;Jong Ho Lee;Siyoung Lee;Saerok Shim;Tam T. Nguyen;Jihyeong Hwang;Heijun Kim;Yeo-Ok Choi;Jaewoo Hong;Suyoung Bae;Hyunjhung Jhun;Hokee Yum;Youngmin Lee;Edward D. Chan;Liping Yu;Tania Azam;Yong-Dae Kim;Su Cheong Yeom;Kwang Ha Yoo;Lin-Woo Kang;Kyeong-Cheol Shin;Soohyun Kim
    • IMMUNE NETWORK
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    • v.20 no.5
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    • pp.41.1-41.11
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    • 2020
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) is a positive-sense single-stranded RNA (+ssRNA) that causes coronavirus disease 2019 (COVID-19). The viral genome encodes twelve genes for viral replication and infection. The third open reading frame is the spike (S) gene that encodes for the spike glycoprotein interacting with specific cell surface receptor - angiotensin converting enzyme 2 (ACE2) - on the host cell membrane. Most recent studies identified a single point mutation in S gene. A single point mutation in S gene leading to an amino acid substitution at codon 614 from an aspartic acid 614 into glycine (D614G) resulted in greater infectivity compared to the wild type SARS-CoV2. We were interested in investigating the mutation region of S gene of SARS-CoV2 from Korean COVID-19 patients. New mutation sites were found in the critical receptor binding domain (RBD) of S gene, which is adjacent to the aforementioned D614G mutation residue. This specific sequence data demonstrated the active progression of SARS-CoV2 by mutations in the RBD of S gene. The sequence information of new mutations is critical to the development of recombinant SARS-CoV2 spike antigens, which may be required to improve and advance the strategy against a wide range of possible SARS-CoV2 mutations.

RPK118, a PX Domain-containing Protein, Interacts with Peroxiredoxin-3 through Pseudo-Kinase Domains

  • Liu, Lungling;Yang, Chenyi;Yuan, Jian;Chen, Xiujuan;Xu, Jianing;Wei, Youheng;Yang, Jingchun;Lin, Gang;Yu, Long
    • Molecules and Cells
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    • v.19 no.1
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    • pp.39-45
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    • 2005
  • RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking.