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Isolation and Characterization of Terpene Synthase Gene from Panax ginseng

  • Kim, Yu-Jin;Ham, Ah-Rom;Shim, Ju-Sun;Lee, Jung-Hye;Jung, Dae-Young;In, Jun-Gyo;Lee, Bum-Soo;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.32 no.2
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    • pp.114-119
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    • 2008
  • Terpene synthase plays a key role in biosynthesis of triterpene saponins (ginsenosides) and is intermediate in the biosynthesis of a number of secondary metabolites. A terpene synthase (PgTPS) cDNA was isolated and characterized from the root of Panax ginseng c.A. Meyer. The deduced amino acid sequence of PgTPS showed a similarity with A. deliciosa (AAX16121) 61%, V. vinifera (AAS66357) 61%, L. hirsutum (AAG41891) 55%, M. truncatula (AAV36464) 52%. And the segment of a terpene synthase gene was amplified by reverse transcriptase-polymerase chain reaction (RTPCR). We studied expression of terpene synthase under stressful conditions like chilling, salt, UV, and heavy metal stress treatment. Expression of PgTPS was increased gradually after exposure to stresses such as chilling, salt, and UV illumination. But its transcription seems to be reduced by cadmium and copper treatment.

The Effect of Modified Atmosphere Packaging and Addition of Rosemary Extract, Sodium Acetate and Calcium Lactate Mixture on the Quality of Pre-cooked Hamburger Patties during Refrigerated Storage

  • Muhlisin, Muhlisin;Kang, Sun Moon;Choi, Won Hee;Lee, Keun Taik;Cheong, Sung Hee;Lee, Sung Ki
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.1
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    • pp.134-142
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    • 2013
  • The effect of modified atmosphere packaging (MAP; 30% $CO_2$+70% $N_2$ or 100% $N_2$) and an additive mixture (500 ppm rosemary extract, 3,000 ppm sodium acetate and 1,500 ppm calcium lactate) on the quality of pre-cooked hamburger patties during storage at $5^{\circ}C$ for 14 d was evaluated. The addition of the additive mixture reduced aerobic and anaerobic bacteria counts in both 30% $CO_2$-MAP (30% $CO_2$+70% $N_2$) and 100% $N_2$-MAP (p<0.05). The 30% $CO_2$-MAP was more effective to suppress the microbial growth than 100% $N_2$-MAP, moreover the 30% $CO_2$-MAP combined with additive mixture resulted in the lowest bacterial counts. The hamburger patties with additive mixture showed lower CIE $L^*$ and CIE $a^*$, and higher CIE $b^*$ than those with no additive mixture. The 30% $CO_2$-MAP tended to decrease the TBARS during storage regardless of the addition of additives. The use of 30% $CO_2$-MAP in combination with additives mixture was effective for maintaining the quality and extending the shelf-life of pre-cooked hamburger patties.

Stability and Sun Protection Efficacy of Sunscreens Based on the Solubility and a Combination of Organic UV Absorbers (유기 자외선 흡수제의 조합과 용해도에 따른 자외선 차단 효율의 비교 및 안정성에 관한 연구)

  • Yeon, Jae Young;Hong, Seung Deok;Choi, Se Bum;Kim, Ta Gon;Lee, Cheong Hee;Lee, Sang Gil;Pyo, Hyeong Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.189-199
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    • 2015
  • In this study, we have investigated the stability of sunscreens based on the solubility of organic UV absorbers in the oil and sun protection efficacy of the products composed of a combination of organic UV absorbers to develop more stable and efficient sunscreen products. Results showed that the solubility of the organic UV absorber and stability were varied depending on the type, storage conditions and concentration of oil. It was also observed from the products in the emulsion type. Various UV absorbances were determined to the products composed of the combination of organic UV absorbers. In some combinations, a synergistic effect was observed to make an increase in absorbance compared to a single component. In other cases, specific synergistic effect was displayed only when combined with the particular component. In addition, the storage condition also affected the sunscreen efficacy. In conclusion, this study confirmed that there are various factors which could affect the UV-blocking efficiency of sunscreen products.

THE EFFECT OF MOUTH RINSE PRODUCTS CONTAINING DEEP SEA WATER (해양심층수를 이용한 구강청정제의 효과)

  • Kim, Seon-Ah;Jang, Hae-Jin;Yoo, Yung-Geun;Chu, Yong-Shik;Park, Yang-Ho;Park, Jun-Woo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.33 no.6
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    • pp.601-608
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    • 2007
  • The aim of this study was to evaluate the effect of mouthrinse products containing deep sea water. We used original deep sea water(DSW) and processed deep sea water desalinated by reverse osmosis at one time(DDW-1), by reverse osmosis at two times(DDW-2) and concentrated by reverse osmosis(CDW). We made 2 kinds of mouthrinse products containing CDW and other agents for smell and taste and one product without deep sea water. The negative control was distilled water. In vivo study, the dental plaque index scores and the gingival index scores were reduced after 4 weeks mouthrinsing three times daily with 4 kinds of deep sea water and 3 kinds of mouthrinse products(p<0.05). The pH of dental plaque in 1 minute after mouthrinsing was not higher than 5.5 in all solutions, but the pH in 20 minutes after mouthrinsing was higher than 5.7 in DSW, CDW and 3 kinds of products which had higher mineral contents. In vitro study, the mouthrinse solutions containing the higher mineral contents were also the more effective in reduction of methyl mercaptan which is one of the causes of halitosis. The 2 kinds of products containing deep sea water killed Streptococcus mutans(ATCC 25175) in culture plates in one minute. These results indicate the usability of deep sea water in mouthrinses for oral hygiene management.

Flavonoids from Epimedium koreanum (음양곽의 Flavonoid 성분에 관한 연구)

  • Kang, Sam-Sik;Shin, Kuk-Hyun;Chung, Sun-Gan;Cho, Eui-Hwan
    • Korean Journal of Pharmacognosy
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    • v.19 no.2
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    • pp.93-96
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    • 1988
  • Three flavonoids were isolated from Epimedium koreanum and identified as quercetin(1), $anhydroicaritin-3-O-{\alpha}-rhamnoside(2)$ and icariin(3) by spectroscopic methods. The former two compounds are the first isolation from this plant.

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The Development of Apparel Worksheet System Using PDM Functions (PDM 기능(機能)을 이용(利用)한 의류(衣類) 작업사양서(作業仕樣書) 시스템의 개발(開發))

  • Park, Joon-Hee;You, Jee-Sun
    • Journal of Fashion Business
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    • v.7 no.5
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    • pp.32-44
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    • 2003
  • In apparel industry, the worksheet or product specification document has the function of products design. Drawing up worksheet exactly is important to quality control of apparel products. Every company needs to manage the product data of design-centric to establish TQM environment. In order to control the quality data of design-centric, an apparel worksheet system was developed by using the function of product data management system.

Quality Analysis of SAR Image

  • Lee, Young-Ran;Kwak, Sung-Hee;Shin, Dong-Seok;Park, Won-Kyu
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.628-630
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    • 2003
  • Synthetic Aperture Radar(SAR) is an active microwave instrument that performs high-resolution observation under almost all weather condition. Research and algorithms have been proposed to process radar signal and to increase the quality of SAR products. In fact, many complicated steps are involved in order to generate a SAR image product. The purpose of this paper is to derive quality assessment procedures and define important test parameters in each procedure inside a SAR processor. Thus those test parameter values indicate the quality of SAR image products and verify the processor's performance. Moreover, required procedures to correct and handle errors which are indicated during the assessment are also presented.

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Marketing Strategies for Promotion Policy of Environmentally Friendly Farm and Organic Products (친환경유기농산물 소비촉진을 위한 마케팅 전략)

  • Yoo, Duck-Ki
    • Korean Journal of Organic Agriculture
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    • v.16 no.4
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    • pp.391-408
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    • 2008
  • The overall focus of this study was to identify marketing and promotion strategies that can maintain and enhance environmentally friendly farm and organic production and processing activities. This is particularly important as the volume of environmentally friendly farm and organic produce that is generally available is increasing, and significant players, such as Organic Marketing Initiatives(OMI) and conventional marketing co-operatives, are becoming involved in the market. The rapid increase in production of environmentally friendly farm products and organic food is creating new and more complex challenges for marketing, from vegetable box schemes large co-operatives supplying the precise quality and volume required by supermarkets. A possible strategy for many farmers is to co-operate in regionally or nationally operating marketing initiatives. The main objective of any public relations(PR) activity should to enhance publicity and to improve an OMIs image. No promotion, no perception of OMI performance.

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HYDROLYZED GINSENG-SAPONIN QUATERNARY; A NOVEL CONDITIONING AGENT FOR HAIR CARE PRODUCTS

  • Kim, Young-Dae;Kim, Chang-Kew;Lee, Chung-Nam;Ha, Byung-Jo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.14 no.1
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    • pp.16-37
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    • 1988
  • A new quaternary ammonium compound, hydrolyzed ginseng-sapoin quaternary (HGSQ), from hydrolyzed Korean ginseng-saponin and 2, 3-epoxypropyltrimethyl ammonium chloride has been developed as a conditioning agent for hair care products. This structure has the hydrophilic group from the introduced cationic and the hydrophobic group from the aglycone of ginseng saponin. Its properties: surface tension, conductivity, critical micelle concentration, eye irritation, sorption onto hair, force reduction (%) for 20% extension and moisture retention effect comparing with the commercial standards. Also half-head tests of HGSQ-containing shampoo were carried out to compare the conditioning effects in shampoos.

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