• Biomedical Science Letters
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• v.23 no.3
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• pp.223-229
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• 2017

Fungal infections by human pathogenic fungi are increasing globally in elderly, children and immune suppressed or deficient patients. Aspergillus fumigatus is one of the well-known pathogenic fungi and causes aspergilloses in human world widely. However, current identification and classification methods based on its phenotypic characteristics still have limitations. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to analyze genetic variations of A. fumigatus clinical isolates, a total of six housekeeping genes were amplified by PCR using specific primer pairs and multi-locus sequence typing (MLST) assay. Results from phylogenetic tree analysis showed that most A. fumigatus strains (88.9%) from respiratory specimens were classified into cluster A and B, and approximately half of A. fumigatus strains (46%) from non-respiratory specimens were classified into cluster C and D. Although the sample size was limited, genetic characteristics of A. fumigatus clinical isolates according to their origins were very similar and well-correlated with other clinical data.

• Journal of the Korean Society of Clothing and Textiles
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• v.26 no.11
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• pp.1593-1604
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• 2002

The purpose of the study were to find how the trend had an effect on a buying motivation, a selecting standard, a frequency of use, a purchase place of cosmetics, makeup and the distinctive quality of social strata of regarding a clothing shopping orientation. The subjects of investigation were the women in twenties who live in Seoul and Chonnam province. This study had been done from May to July 2001, and 660 questionnaires were used for analysis. The measuring tools used were the items of matters of preceding studies and developed ones by researchers on which questionnaires were prepared. Factor and cluster analysis and Duncan's multiple range test, ANOVA, x$^2$-test, frequency, and percentage as analysis methods were used through SPSS. The results of the study were as follows. The clothing shopping orientation divided five shopping orientations such as ostentatious style, enjoyable style, economical style, and prudent style, and an attachment style for special brands and shops. On the basis of this result, the groups 20's females were classified into low groups by the cluster analysis; economical shopping group, indifferent shopping group, conspicuous shopping group, and recreational shopping group. In the case of cosmetics purchasing motives, evaluation criteria of cosmetics, stores selection criteria were found according to shopping orientation subdivision, and there was the significant difference between the groups in frequency of use, a purchase place of cosmetics.

• Journal of the Korea Society of Computer and Information
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• v.13 no.3
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• pp.1-13
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• 2008

Gestwicki et. al. proposed the technique that detects and predicts the structure of object diagrams from class diagrams to get the improved drawing of object graphs. Their approach finds two predictable structures - recursive clusters and leaf clusters from class diagrams. Their approach is not practical to be applied to real programs, because the starting point is class diagram. In this work, we improve and implement their technique to apply to Java classes. We obtain the structure and relationships of classes and clusters extracted from class files. This information can be provided and used as a template of object graphs for Java execution visualization tools like JIVE.

• Korean Journal of Microbiology
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• v.36 no.4
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• pp.249-253
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• 2000

The genetic diversity among Korean cyanobacteria was assessed by restriction fragment length polymorphism(RFLP) analysis of PCR products from the phycocyanin locus. Strains of all the genera tested were successfully amplified, and the size of amplified fragments was approximately 700bp. The restriction patterns generated by AluI, MspI, and HaeIII were conserved for strains within each of genera studied and were specific to the genus level. Intrageneric delineation of strains was revealed by the enzyme, CfoI for members of genera Anabeana and Synechocystis. Phenogram derived from the different RFLP patterns revealed a coherent cluster among Anabeana, Chlorogloea, and Synechocystis strains. PC-RFLP methods provided useful tools for classification of cyanobacteria.

• School Mathematics
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• v.15 no.1
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• pp.15-35
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• 2013

The purpose of the research is to categorize math learners into pattern through those tools that distinguish math learning style for middle school students. On the ground of survey for 976 middle school students, the fact that there are 16 different math learning style at the result of cluster analysis is confirmed and the results are compared and analyzed previous research. Also according to the each constituent of math learning style, dissimilarity of distribution about learner of different sexes and grades are analyzed. It's helpful to understanding the whole characteristics of learners regarding math learning to figure out their cognitive and affective learning styles through the tools to distinguish their math learning styles.

• Earthquakes and Structures
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• v.9 no.5
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• pp.957-981
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• 2015

Earthquakes occur as a cluster in many regions around the world where complex fault systems exist. The repeated shaking usually induces accumulative damage to affected structures. Damage accumulation in structural systems increases their level of degradation in stiffness and also reduces their strength. Many existing analytical tools of modeling RC structures lack the salient damage features that account for stiffness and strength degradation resulting from repeated earthquake loading. Therefore, these tools are inadequate to study the response of structures in regions prone to multiple earthquakes hazard. The objective of this paper is twofold: (a) develop a tool that contains appropriate damage features for the numerical analysis of RC structures subjected to more than one earthquake; and (b) conduct a parametric study that investigates the effects of multiple earthquakes on the response of RC moment resisting frame systems. For this purpose, macroscopic constitutive models of concrete and steel materials that contain the aforementioned damage features and are capable of accurately capturing materials degrading behavior, are selected and implemented into fiber-based finite element software. Furthermore, finite element models that utilize the implemented concrete and steel stress-strain hysteresis are developed. The models are then subjected to selected sets of earthquake sequences. The results presented in this study clearly indicate that the response of degrading structural systems is appreciably influenced by strong-motion sequences in a manner that cannot be predicted from simple analysis. It also confirms that the effects of multiple earthquakes on earthquake safety can be very considerable.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1722-1731
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• 2021

The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.

• Food Science of Animal Resources
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• v.42 no.4
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• pp.672-688
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• 2022

The objective of this study was to explore the potential of front face fluorescence spectroscopy (FFFS) as rapid, non-destructive and inclusive technique along with multi-variate analysis for predicting meat adulteration. For this purpose (FFFS) was used to discriminate pure minced beef meat and adulterated minced beef meat containing (1%, 2%, 3%, 4%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%) of chicken meat as an adulterant in uncooked beef meat samples. Fixed excitation (290 nm, 322 nm, and 340 nm) and fixed emission (410 nm) wavelengths were used for performing analysis. Fluorescence spectra were acquired from pure and adulterated meat samples to differentiate pure and binary mixtures of meat samples. Principle component analysis, partial least square regression and hierarchical cluster analysis were used as chemometric tools to find out the information from spectral data. These chemometric tools predict adulteration in minced beef meat up to 10% chicken meat but are not good in distinguishing adulteration level from 1% to 5%. The results of this research provide baseline for future work for generating spectral libraries using larger datasets for on-line detection of meat authenticity by using fluorescence spectroscopy.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.4
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• pp.16-25
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• 2003

A parallel computing environment based on Windows operating system was constructed and a performance test was mode in comparison with Linux based systems. The Windows 2000 cluster was composed with servers and clients connected by Fast-ethernet, within which two sub-clusters may operates together or separately. Compaq Visual Fortran complier and two MPI libraries, MPICH.NT.1.2.2 and NT-MPICHNT.1.2 were installed as computing tools. Parallel computing performance tests were carried out using two-dimensional preconditioned Navier-Stokes code to examine the dependency on the number of processors, problem size and MPI libraries, those were compared with results from Linux clusters. Results shows that a cluster based on the user-friendly Windows operating system is also useful for the parallel computing and has good performance comparable to the previous Linux clusters.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.18 no.2
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• pp.122-132
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• 2008

The purpose of this study is to present the basic materials of a developmental counterplan by assessing prevalence of musculoskeletal symptoms and characteristics of work-related ergonomic risk factors. The prevalence of musculoskeletal symptoms of 64 vineyard workers was investigated by using a self-reported questionnaire. And the vineyard workers' tasks were also collected and analyzed during 9-month period by observing and videotaping methods to identify the ergonomic risk factors. The prevalence rate of musculoskeletal symptoms of female(84.4%) was much higher than that of male(62.5%) and a total prevalence rate was 73.4%. There was no different prevalence of musculoskeletal symptoms between age over 60 years and under 60 years. The prevalence rates of musculoskeletal complaints for each body part were 48.4%, 42.2%, and 35.9% for the wrist, legs/knee, and shoulder, respectively. In postural risk analysis, 7 works(REBA score) represented the high risk tasks such as delivering harvest boxed(12), picking cluster(11) and so on. The main works(exposure score) were orderly ranked as picking cluster(1590), pruning branch(388), and cluster thinning(327). The risk factors of vineyard work were identified as follows: shoulder flexion(${\geq}45^{\circ}$), wrist Flex./Ext.(${\geq}15^{\circ}$), hand force(power/pinch-grip), and prolonged standing(${\geq}4hr$). The engineering solutions including an improvement of hand tools, working process, and working environment should be applied to the high risky tasks in order to resolve the ergonomic problems. The administrative solutions such as improving a distribution of resting time, an exercise cure, an early recognition of symptoms and rehabilitation might be another solution for reducing musculoskeletal symptoms in vineyard workers.