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Genetic Variations of Aspergillus fumigatus Clinical Isolates from Korea

  • Kim, Sunghyun (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Ma, Pan-Gon (Department of Clinical Laboratory Science, Daegu Health College) ;
  • Park, Young-Seok (Department of Emergency Medical Services, Sunmoon University) ;
  • Yu, Young-Bin (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University) ;
  • Hwang, Kyu Jam (Pathogen Resource TF, Center for Infectious Disease, National Research Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Kim, Young Kwon (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University)
  • Received : 2017.08.22
  • Accepted : 2017.09.18
  • Published : 2017.09.30

Abstract

Fungal infections by human pathogenic fungi are increasing globally in elderly, children and immune suppressed or deficient patients. Aspergillus fumigatus is one of the well-known pathogenic fungi and causes aspergilloses in human world widely. However, current identification and classification methods based on its phenotypic characteristics still have limitations. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to analyze genetic variations of A. fumigatus clinical isolates, a total of six housekeeping genes were amplified by PCR using specific primer pairs and multi-locus sequence typing (MLST) assay. Results from phylogenetic tree analysis showed that most A. fumigatus strains (88.9%) from respiratory specimens were classified into cluster A and B, and approximately half of A. fumigatus strains (46%) from non-respiratory specimens were classified into cluster C and D. Although the sample size was limited, genetic characteristics of A. fumigatus clinical isolates according to their origins were very similar and well-correlated with other clinical data.

Keywords

References

  1. Anderson MJ, Gull K, Denning DW. Molecular typing by random amplification of polymorphic DNA and M13 Southern hybridization of related paired isolates of Aspergillus fumigatus. Journal of Clinical Microbiology. 1996. 34: 87-93.
  2. Bain JM, Tavanti A, Davidson AD, Jacobsen MD, Shaw D, Gow NA, Odds FC. Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus. Journal of Clinical Microbiology. 2007. 45: 1469-1477. https://doi.org/10.1128/JCM.00064-07
  3. Balajee SA, Gribskov JL, Hanley E, Nickle D, Marr KA. Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus. Eukaryotic Cell. 2005. 4: 625-632. https://doi.org/10.1128/EC.4.3.625-632.2005
  4. Balajee SA, Nickle D, Varga J, Marr KA. Molecular studies reveal frequent misidentification of Aspergillus fumigatus by morphotyping. Eukaryotic Cell. 2006. 5: 1705-1712. https://doi.org/10.1128/EC.00162-06
  5. Bougnoux ME, Tavanti A, Bouchier C, Gow NA, Magnier A, Davidson AD, Maiden MC, D'Enfert C, Odds FC. Collaborative consensus for optimized multilocus sequence typing of Candida albicans. Journal of Clinical Microbiology. 2003. 41: 5265-5266. https://doi.org/10.1128/JCM.41.11.5265-5266.2003
  6. Debourgone A, Gueidan C, Henneqyuin C, Contet-Audonneau N, de Hoog S, Machouart M. Development of a new MLST scheme for differentiation of Fusarium solani species complex (FSSC) isolates. Journal of Microbiological Methods. 2010. 82: 319-323. https://doi.org/10.1016/j.mimet.2010.07.008
  7. De Hoog GS, Guarro J, Gene J, Figueras MJ. Atlas of clinical fungi, Centraalbureau voor Schimmelcultures 2nd ed. 2000.
  8. Dodgson AR, Pujol C, Denning DW, Soll DR, Fox AJ. Multilocus sequence typing of Candida glabrata reveals geographically enriched clades. Journal of Clinical Microbiology. 2003. 41:5709-5717. https://doi.org/10.1128/JCM.41.12.5709-5717.2003
  9. Gil-Lamaignere C, Roilides E, Hacker J, Muller FM. Molecular typing for fungi a critical review of the possibilities and limitations of currently and future methods. Clinical Microbiology and Infection. 2003. 9: 173-185.
  10. Halliday CL, Carter DA. Clonal reproduction and limited dispersal in an environmental population of Cryptococcus neoformans var. gattii isolates from Australia. Journal of Clinical Microbiology. 2003. 41: 703-711. https://doi.org/10.1128/JCM.41.2.703-711.2003
  11. Jacobsen MD, Gow NA, Maiden MC, Shaw DJ, Odds FC. Strain typing and determination of population structure of Candida krusei by multilocus sequence typing. Journal of Clinical Microbiology. 2007. 45: 317-323. https://doi.org/10.1128/JCM.01549-06
  12. Jiang S, Jiang L, Shan F, Zhang X, Song M. Two cases of endobronchial aspergilloma with lung cancer: a review the literature of endobronchial aspergilloma with underlying malignant lesions of the lung. International Journal of Clinical and Experimental Medicine. 2015. 8: 17015-17021.
  13. Litvintseva AP, Thakur R, Vilgalys R, Mitchell TG. Multilocus sequence typing reveals three genetic subpopulations of Cryptococcus neoformans var. grubii (serotype A), including a unique population in Botswana. Genetics. 2006. 172: 2223-2238.
  14. Neofytos D, Fishman JA, Horn D, Anassie E, Chang CH, Olyaei A, Pfaller M, Steinbach WJ, Webster KM, Marr KA. Epidemiology and outcome of invasive fungal infections in solid organ transplant recipients. Transplant Infectious Disease. 2010. 12:220-229. https://doi.org/10.1111/j.1399-3062.2010.00492.x
  15. Reichenberger F, Habicht JM, Gratwohl A, Tamm M. Diagnosis and treatment of invasive pulmonary aspergillosis in neutropenic patients. European Respiratory Journal. 2002. 19: 743-755. https://doi.org/10.1183/09031936.02.00256102
  16. Tavanti A, Davidson AD, Johnson EM, Maiden MC, Shaw DJ, Gow NA, Odds FC. Multilocus sequence typing for differentiation of strains of Candida tropicalis. Journal of Clinical Microbiology. 2005. 43: 5593-5600. https://doi.org/10.1128/JCM.43.11.5593-5600.2005
  17. Warnock DW. Trends in the epidemiology of invasive fungal infections. Japanese Journal of Medical Mycology. 2007. 48:1-12. https://doi.org/10.3314/jjmm.48.1
  18. Yoon HJ, Choi HY, Kim YK, Song YJ, Ki M. Prevalence of fungal infections using National Health Insurance data from 2009-2013, South Korea. Epidemiology and Health. 2004. 36: 1-10.