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http://dx.doi.org/10.15616/BSL.2017.23.3.223

Genetic Variations of Aspergillus fumigatus Clinical Isolates from Korea  

Kim, Sunghyun (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Ma, Pan-Gon (Department of Clinical Laboratory Science, Daegu Health College)
Park, Young-Seok (Department of Emergency Medical Services, Sunmoon University)
Yu, Young-Bin (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University)
Hwang, Kyu Jam (Pathogen Resource TF, Center for Infectious Disease, National Research Institute of Health, Korea Center for Disease Control and Prevention)
Kim, Young Kwon (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University)
Publication Information
Biomedical Science Letters / v.23, no.3, 2017 , pp. 223-229 More about this Journal
Abstract
Fungal infections by human pathogenic fungi are increasing globally in elderly, children and immune suppressed or deficient patients. Aspergillus fumigatus is one of the well-known pathogenic fungi and causes aspergilloses in human world widely. However, current identification and classification methods based on its phenotypic characteristics still have limitations. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to analyze genetic variations of A. fumigatus clinical isolates, a total of six housekeeping genes were amplified by PCR using specific primer pairs and multi-locus sequence typing (MLST) assay. Results from phylogenetic tree analysis showed that most A. fumigatus strains (88.9%) from respiratory specimens were classified into cluster A and B, and approximately half of A. fumigatus strains (46%) from non-respiratory specimens were classified into cluster C and D. Although the sample size was limited, genetic characteristics of A. fumigatus clinical isolates according to their origins were very similar and well-correlated with other clinical data.
Keywords
Aspergillus fumigatus; Clinical isolates; Genetic variations; Multi-locus sequence typing; Genotypes;
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