• Title/Summary/Keyword: Closeness

Search Result 459, Processing Time 0.022 seconds

Coproduction of Thermostable Amylase and ${\beta}$-Galactosidase Enzymes by Geobacillus stearothermophilus SAB-40: Application of Plackett-Burman Design to Evaluate Culture Requirements Affecting Enzyme Production

  • Soliman, Nadia A.
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.4
    • /
    • pp.695-703
    • /
    • 2008
  • A locally isolated thermophile, Geobacillus sp. SAB-40, producing thermostable extracellular amylase constitutively and an induced intracellular ${\beta}$-galactosidase was characterized and identified based on 16S rRNA sequencing. A phylogenetic analysis then revealed its closeness to Geobacillus stearothermophilus. To evaluate the effect of the culture conditions on the coproduction of both enzymes by G stearothermophilus SAB-40, a Plackett-Burman fractional factorial design was applied to determine the impact of twenty variables. Among the tested variables, $CaCI_2$, the incubation time, $MgSO_4{\cdot}7H_2O$, and tryptone were found to be the most significant for encouraging amylase production. Lactose was found to promote ${\beta}$-galactosidase production, whereas starch had a significantly negative effect on lactase production. Based on a statistical analysis, a preoptimized medium attained the maximum production of amylase and ${\beta}$-galactosidase at 23.29 U/ml/ min and 12,958 U/mg biomass, respectively, which was 3-and 2-fold higher than the yield of amylase and lactase obtained with the basal medium, respectively.

Relationship among Ginsenosides of Panax ginseng Root under the Variation of Mineral Nutrients (무기영양변화에서 인삼근Ginsenoside의 상호관계)

  • Lee, Mee-Kyoung;Min, Jin-Sook;Park, Hoon
    • Journal of Ginseng Research
    • /
    • v.10 no.1
    • /
    • pp.101-107
    • /
    • 1986
  • Relationships among ginsenosides, panaxadiol(PD), panaxatriol(PT), and total saponin(TS) in Panax ginseng root (2nd Year) grown with culture solotion different in nitrogen, phosphorus and potassium level were analyzed by simple correlation, multiple regression and standard partial regression coefficient. The closeness between ginsenosides by simple correlation was closely related with the similarity of molecular structure. The content of PT was much attributed to Re and Rg1. The contribution order of ginsenosides for PD was Rb1>Rb2$\geq$Rd>Rc. There was significant positive correlation between PT and PD but PD increased more rapidly than PT. Thus total saponin depended much on PD and PT/PD decreased with the increase of total saponin content. All ginsenosides, especially Re showed decreasing tendency with the increase of root weight.

  • PDF

Estimation of bridge displacement responses using FBG sensors and theoretical mode shapes

  • Shin, Soobong;Lee, Sun-Ung;Kim, Yuhee;Kim, Nam-Sik
    • Structural Engineering and Mechanics
    • /
    • v.42 no.2
    • /
    • pp.229-245
    • /
    • 2012
  • Bridge vibration displacements have been directly measured by LVDTs (Linear Variable Differential Transformers) or laser equipment and have also been indirectly estimated by an algorithm of integrating measured acceleration. However, LVDT measurement cannot be applied for a bridge crossing over a river or channel and the laser technique cannot be applied when the weather condition is poor. Also, double integration of accelerations may cause serious numerical deviation if the initial condition or a regression process is not carefully controlled. This paper presents an algorithm of estimating bridge vibration displacements using vibration strains measured by FBG (Fiber Bragg Grating) sensors and theoretical mode shapes of a simply supported beam. Since theoretically defined mode shapes are applied, even high modes can be used regardless of the quality of the measured data. In the proposed algorithm, the number of theoretical modes is limited by the number of sensors used for a field test to prevent a mathematical rank deficiency from occurring in computing vibration displacements.89The proposed algorithm has been applied to various types of bridges and its efficacy has been verified. The closeness of the estimated vibration displacements to measured ones has been evaluated by computing the correlation coefficient and by comparing FRFs (Frequency Response Functions) and the maximum displacements.

Numerical modelling of FRP strengthened RC beam-column joints

  • Mahini, Seyed S.;Ronagh, Hamid R.
    • Structural Engineering and Mechanics
    • /
    • v.32 no.5
    • /
    • pp.649-665
    • /
    • 2009
  • This paper reports part of a comprehensive research study conducted at the University of Queensland on the ability of CFRP web-bonded systems in strengthening an exterior beam-column joint subjected to monotonic loads. One 1/2.2 scaled plain and four CFRP repaired/retrofitted joints subjected to monotonic loads were analysed using the nonlinear finite-element program ANSYS and the results were calibrated against experiments. The ANSYS model was employed in order to account for tension stiffening in concrete after cracking and a modified version of the Hognestad's model was used to model the concrete compressive strength. The stress-strain properties of main steel bars were modelled using multilinear isotropic hardening model and the FRPs were modelled as anisotropic materials. A perfect bond was assumed as nodes were shared between adjacent elements irrespective of their type. Good agreement between the numerical predictions and the experimental observation of the failure mechanisms for all specimens were observed. Closeness of these results proved that the numerical analysis can be used by design engineers for the analysis of web-bonded FRP strengthened beam-column joints with confidence.

Trend Analysis of Data Mining Research Using Topic Network Analysis

  • Kim, Hyon Hee;Rhee, Hey Young
    • Journal of the Korea Society of Computer and Information
    • /
    • v.21 no.5
    • /
    • pp.141-148
    • /
    • 2016
  • In this paper, we propose a topic network analysis approach which integrates topic modeling and social network analysis. We collected 2,039 scientific papers from five top journals in the field of data mining published from 1996 to 2015, and analyzed them with the proposed approach. To identify topic trends, time-series analysis of topic network is performed based on 4 intervals. Our experimental results show centralization of the topic network has the highest score from 1996 to 2000, and decreases for next 5 years and increases again. For last 5 years, centralization of the degree centrality increases, while centralization of the betweenness centrality and closeness centrality decreases again. Also, clustering is identified as the most interrelated topic among other topics. Topics with the highest degree centrality evolves clustering, web applications, clustering and dimensionality reduction according to time. Our approach extracts the interrelationships of topics, which cannot be detected with conventional topic modeling approaches, and provides topical trends of data mining research fields.

Architectural Expression of Light Appeared in Museums Designed by Henri E. Ciriani (앙리 시리아니의 박물관 건축에 나타난 빛의 건축적 표현)

  • Kim, Chang-Sung
    • Korean Institute of Interior Design Journal
    • /
    • v.23 no.2
    • /
    • pp.3-11
    • /
    • 2014
  • Light has been considered as one of the most important elements in architectural design. Light provides occupants in buildings a lot of architectural experiences by interrelating the space, shape and other design elements. Especially, natural light is the valuable source to create the better indoor space compared to artificial light. It is a sustainable energy source and offers a more natural environment. It also enables occupants to perceive the form and depth of space. In general. many of architects including Henri Ciriani have tried to design buildings with natural light expecting optimum indoor environment. Therefore, this paper tried to examine the works of Henri Ciriani and analyze how to control the light in his works. For this purpose, two museums designed by Henri Ciriani-Arles Museum of Archaeology and Great War Historical Museum in Peronne - were selected to analyze how Henri Ciriani used light in his design phase and applied it to his museum works. According to the results of the study, it has been proved that Henri Ciriani tried to realize a space continuum through the spatial expansion, openness and closeness by natural light and incorporate the architectural form, interior space and exhibition circulation with natural light in order to create innovative exhibition space in museum buildings.

Real-Time Face Detection by Estimating the Eye Region Using Neural Network (신경망 기반 눈 영역 추정에 의한 실시간 얼굴 검출 기법)

  • 김주섭;김재희
    • Proceedings of the IEEK Conference
    • /
    • 2001.06d
    • /
    • pp.21-24
    • /
    • 2001
  • In this paper, we present a fast face detection algorithm by estimating the eye region using neural network. To implement a real time face detection system, it is necessary to reduce search space. We limit the search space just to a few pairs of eye candidates. For the selection of them, we first isolate possible eye regions in the fast and robust way by modified histogram equalization. The eye candidates are paired to form an eye pair and each of the eye pair is estimated how close it is to a true eye pair in two aspects : One is how similar the two eye candidates are in shape and the other is how close each of them is to a true eye image A multi-layer perceptron neural network is used to find the eye candidate region's closeness to the true eye image. Just a few best candidates are then verified by eigenfaces. The experimental results show that this approach is fast and reliable. We achieved 94% detection rate with average 0.1 sec Processing time in Pentium III PC in the experiment on 424 gray scale images from MIT, Yale, and Yonsei databases.

  • PDF

RAPD Polymorphism of Xanthoceras sorbifolium Bunge from Three Plantations of China (중국 3곳 문관나무 재배집단의 RAPD 다형성)

  • An, Chanhoon;Lee, Hyunseok;Yin, Zhiyang;Yi, Jae-Seon
    • Journal of Forest and Environmental Science
    • /
    • v.28 no.4
    • /
    • pp.242-246
    • /
    • 2012
  • The variation in random amplified polymorphic DNA (RAPD) markers were examined for Xanthoceras sorbifolium Bunge seeds from three plantations (Inner Mongolia [IM], Liaoning [LN], and Shandong [SD] province) in China. Mean genetic variation was measured by polymorphism percentage (42.10%) and expected heterozygosity (He=1.27). Among three populations, Shandong showed the highest values both in polymorphism percentage and heterozygosity (p=57.89; Ho=1.58; and He=1.37). Total genetic diversity value, based on the total loci, was estimated as total genetic diversity of the species (Ht)=0.27 and mean within-population genetic diversity (Hs)=0.16. UPGMA cluster analysis showed the genetic closeness between Inner Mongolia and Liaoning population, but that Shandong seems to be the separate population.

A Study On Male-To-Female Voice Conversion (남녀 음성 변환 기술연구)

  • Choi Jung-Kyu;Kim Jae-Min;Han Min-Su
    • Proceedings of the Acoustical Society of Korea Conference
    • /
    • spring
    • /
    • pp.115-118
    • /
    • 2000
  • Voice conversion technology is essential for TTS systems because the construction of speech database takes much effort. In this paper. male-to-female voice conversion technology in Korean LPC TTS system has been studied. In general. the parameters for voice color conversion are categorized into acoustic and prosodic parameters. This paper adopts LSF(Line Spectral Frequency) for acoustic parameter, pitch period and duration for prosodic parameters. In this paper. Pitch period is shortened by the half, duration is shortened by $25\%, and LSFs are shifted linearly for the voice conversion. And the synthesized speech is post-filtered by a bandpass filter. The proposed algorithm is simpler than other algorithms. for example, VQ and Neural Net based methods. And we don't even need to estimate formant information. The MOS(Mean Opinion Socre) test for naturalness shows 2.25 and for female closeness, 3.2. In conclusion, by using the proposed algorithm. male-to-female voice conversion system can be simply implemented with relatively successful results.

  • PDF

Optimal design of reinforced concrete plane frames using artificial neural networks

  • Kao, Chin-Sheng;Yeh, I-Cheng
    • Computers and Concrete
    • /
    • v.14 no.4
    • /
    • pp.445-462
    • /
    • 2014
  • To solve structural optimization problems, it is necessary to integrate a structural analysis package and an optimization package. There have been many packages that can be employed to analyze reinforced concrete plane frames. However, because most structural analysis packages suffer from closeness of systems, it is very difficult to integrate them with optimization packages. To overcome the difficulty, we proposed a possible alternative, DAMDO, which integrates Design, Analysis, Modeling, Definition, and Optimization phases into an integration environment as follows. (1) Design: first generate many possible structural design alternatives. Each design alternative consists of many design variables X. (2) Analysis: employ the structural analysis software to analyze all structural design alternatives to obtain their internal forces and displacements. They are the response variables Y. (3) Modeling: employ artificial neural networks to build the models Y=f(X) to obtain the relationship functions between the design variables X and the response variables Y. (4) Definition: employ the design variables X and the response variables Y to define the objective function and constraint functions. (5) Optimization: employ the optimization software to solve the optimization problem consisting of the objective function and the constraint functions to produce the optimum design variables. The RC frame optimization problem was examined to evaluate the DAMDO approach, and the empirical results showed that it can be solved by the approach.