• The Journal of Korean Academy of Prosthodontics
• /
• v.57 no.4
• /
• pp.425-431
• /
• 2019

This clinical case report describes the digital workflow that combines a face scan, cone beam computed tomography and an intraoral scan to visualize the outcome of prosthodontic treatment in the anterior region. This approach improves communication between clinic, laboratory and patients. A patient with healthy general condition came for a restorative treatment to treat post-traumatic central incisors of maxilla. A virtual patient replica was made by incorporating a face scan, cone beam computed tomography and an intraoral scan. Design mockup of definitive restorations was shown to the patient and modified according to the patient's desire. This digital workflow facilitates the fabrication of optimal esthetic restorations, and enhances the predictability of outcome of restorations.

• The Journal of Advanced Prosthodontics
• /
• v.11 no.5
• /
• pp.271-279
• /
• 2019

PURPOSE. The newest technologies for digital implant impression (DII) taking are developing rapidly and showing acceptable clinical results. However, scientific literature is lacking data from clinical studies about the accuracy of DII. The aim of this study was to compare digital and conventional dental implant impressions (CII) in a clinical environment. MATERIALS AND METHODS. Twenty-four fixed zirconia restorations supported by 2 implants were fabricated using conventional open-tray impression technique with splinted transfers (CII group) and scan with Trios 3 IOS (3Shape) (DII group). After multiple verification procedures, master models were scanned using laboratory scanner D800 (3Shape). 3D models from conventional and digital workflow were imported to reverse engineering software and superimposed with high resolution 3D CAD models of scan bodies. Distance between center points, angulation, rotation, vertical shift, and surface mismatch of the scan bodies were measured and compared between conventional and digital impressions. RESULTS. Statistically significant differences were found for: a) inter-implant distance, b) rotation, c) vertical shift, and d) surface mismatch differences, comparing DII and CII groups for mesial and distal implant scan bodies ($P{\leq}.05$). CONCLUSION. Recorded linear differences between digital and conventional impressions were of limited clinical significance with two implant-supported restorations.

• The Journal of the Korean dental association
• /
• v.57 no.9
• /
• pp.524-528
• /
• 2019

With the advances of CAD-CAM (computer-aided design and computer-aided manufacturing) technology, the field of modern clinical dentistry has been dramatically changed. The first step in the digital workflow for tooth-supported dental prosthesis is a data acquisition with intraoral digital or conventional impression techniques. For the accuracy of intraoral digital impression data, the basic principles of conventional impression should be applied. It is necessary to obtain a good visibility with properly-dried field and well-exposed margin of the prepared abutment. Currently, the equi- or supra-gingival finish line can be recommended as an indication for intraoral digital impression. The scan data are generally exported to '.stl' file format, which has only morphological information of black and whitem while '.obj' file format can store data on color and texture.

• The korean journal of orthodontics
• /
• v.52 no.3
• /
• pp.236-245
• /
• 2022

New digital technologies, many involving three-dimensional printing, bring benefits for clinical applications. This article reports on the clinical procedure and fabrication of a skeletally anchored mesialization appliance (Mesialslider) using computer-aided design/computer-aided manufacturing (CAD/CAM) for space closure of a congenitally missing lateral incisor in a 12-year-old female patient. The insertion of the mini-implants and appliance was performed in a single appointment. Bodily movement of the molars was achieved using the Mesialslider. Anchorage loss, such as deviation of the anterior midline or palatal tilting of the anterior teeth, was completely avoided. CAD/CAM facilitates safe and precise insertion of mini-implants. Further, mini-implants can improve patient comfort by reducing the number of office visits and eliminating the need for orthodontic bands and physical impressions.

• Korean Journal of Clinical Laboratory Science
• /
• v.56 no.1
• /
• pp.10-20
• /
• 2024

RNA-sequencing (RNA-seq) is a technique used for providing global patterns of transcriptomes in samples. However, it can only provide the average gene expression across cells and does not address the heterogeneity within the samples. The advances in single-cell RNA sequencing (scRNA-seq) technology have revolutionized our understanding of heterogeneity and the dynamics of gene expression at the single-cell level. For example, scRNA-seq allows us to identify the cell types in complex tissues, which can provide information regarding the alteration of the cell population by perturbations, such as genetic modification. Since its initial introduction, scRNA-seq has rapidly become popular, leading to the development of a huge number of bioinformatic tools. However, the analysis of the big dataset generated from scRNA-seq requires a general understanding of the preprocessing of the dataset and a variety of analytical techniques. Here, we present an overview of the workflow involved in analyzing the scRNA-seq dataset. First, we describe the preprocessing of the dataset, including quality control, normalization, and dimensionality reduction. Then, we introduce the downstream analysis provided with the most commonly used computational packages. This review aims to provide a workflow guideline for new researchers interested in this field.

• The Journal of Advanced Prosthodontics
• /
• v.10 no.6
• /
• pp.430-439
• /
• 2018

The treatment of craniofacial anomalies has been challenging as a result of technological shortcomings that could not provide a consistent protocol to perfectly restore patient-specific anatomy. In the past, wax-up and impression-based maneuvers were implemented to achieve this clinical end. However, with the advent of computer-aided design and computer-aided manufacturing (CAD/CAM) technology, a rapid and cost-effective workflow in prosthetic rehabilitation has taken the place of the outdated procedures. Because the use of implants is so profound in different facets of restorative dentistry, their placement for craniofacial prosthesis retention has also been widely popular and advantageous in a variety of clinical settings. This review aims to effectively describe the well-rounded and interdisciplinary practice of craniofacial prosthesis fabrication and retention by outlining fabrication, osseointegrated implant placement for prosthesis retention, a myriad of clinical examples in the craniofacial complex, and a glimpse of the future of bioengineering principles to restore bioactivity and physiology to the previously defected tissue.

• Korean Journal of Digital Imaging in Medicine
• /
• v.8 no.1
• /
• pp.21-26
• /
• 2006

Hospitals these days are trying to introduce the a practice has recently been generalized in the test or diagnosis process, where test results and images from different test labs are interlinked together. This process is identical to that of physical aspect in EMR process, which computerizes the paper results within the hospital. One of the prerequisites for the process of computerizing test results is the interface between clinical test devices in the test labs. However, due to the variety of prescription inputs, disparity of test result papers, complexity of job in test labs and diversify of interfaces among the different devices, interconnection with the hospital information system is a complicated job. A universal control of clinical test devices which have independent communication protocols has become possible by connecting them with an interface workstation. As for the patients, waiting time for test has been reduced, and, thanks to the synchronized result retrieval system, it has become possible to check the test results on the very day of the test. As a result, the length of hospitalization has been reduced, too. In terms of workflow, as the transfer of charts and transfer of result papers are separated, the embarrassing job of collecting result papers has disappeared. As patients' test appointment and the results processing can be made on-line, extra work for doctors have disappeared. And, thanks to the computerization of test results information management, the job of statistical processing has become convenient.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.33 no.4
• /
• pp.299-306
• /
• 2017

In this case report, immediate loading of an implant-supported single-tooth prosthesis through complete digital workflow. A patient presented for restoration of missing a single tooth in the mandibular first molar. The digital impression was made with intraoral scanner and implant was placed using surgical guide pre-fabricated with pre-operative computed tomography (CT) and scan data. After 1 week later, prefabricated customized abutment and provisional restoration were connected for immediate loading. After 8 weeks later, abutment level impression was taken by intraoral scanner. At 3 months later from implant installation, monolithic zirconia crown were fabricated. This clinical report presents satisfying result in accuracy and patient satisfaction. A completely modeless digital procedure met expectations regarding precision, esthetics, and functionality.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.17
• /
• pp.7935-7941
• /
• 2015

The aim of this study was to implement massive parallel sequencing (MPS) technology in clinical genetics testing. We developed and tested an amplicon-based method for resequencing the BRCA1 and BRCA2 genes on an Illumina MiSeq to identify disease-causing mutations in patients with hereditary breast or ovarian cancer (HBOC). The coding regions of BRCA1 and BRCA2 were resequenced in 96 HBOC patient DNA samples obtained from different sample types: peripheral blood leukocytes, whole blood drops dried on paper, and buccal wash epithelia. A total of 16 random DNA samples were characterized using standard Sanger sequencing and applied to optimize the variant calling process and evaluate the accuracy of the MPS-method. The best bioinformatics workflow included the filtration of variants using GATK with the following cut-offs: variant frequency >14%, coverage ($>25{\times}$) and presence in both the forward and reverse reads. The MPS method had 100% sensitivity and 94.4% specificity. Similar accuracy levels were achieved for DNA obtained from the different sample types. The workflow presented herein requires low amounts of DNA samples (170 ng) and is cost-effective due to the elimination of DNA and PCR product normalization steps.

• The Journal of Korean Academy of Prosthodontics
• /
• v.60 no.4
• /
• pp.354-361
• /
• 2022

The development of digital technology is causing great changes in dentistry. This digital workflow combines various 3D data in the prosthetic treatment area for diagnosis and prosthetic manufacturing. The planned diagnosis and the fabrication of prosthesis in a virtual patient formed by synthesizing digital data can simulate the results of prosthetic treatment more intuitively than conventional methods, thereby increasing the predictability of aesthetic prosthetic treatment. In this case report, functionally and aesthetically satisfied clinical results were obtained by fabricating a fixed partial dentures through a digital workflow on congenital missing teeth in the maxillary anterior region.