• 한국동물생명공학회지
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• 제36권2호
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• pp.106-115
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• 2021

Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

• Journal of Gastric Cancer
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• 제22권3호
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• pp.197-209
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• 2022

Purpose: This study systematically evaluated the implications of advanced age on post-surgical outcomes following gastrectomy for gastric cancer using a national database. Materials and Methods: The 2011-2017 National Inpatient Sample was used to isolate patients who underwent gastrectomy for gastric cancer. From this, the population was stratified into those belonging to the younger age cohort (18-59 years), sexagenarians, septuagenarians, and octogenarians. The younger cohort and each advanced age category were compared in terms of the following endpoints: mortality following surgery, length of hospital stay, charges, and surgical complications. Results: This study included a total of 5,213 patients: 1,366 sexagenarians, 1,490 septuagenarians, 743 octogenarians, and 1,614 under 60 years of age. Between the younger cohort and sexagenarians, there was no difference in mortality (2.27 vs. 1.67%; P=0.30; odds ratio [OR], 1.36; 95% confidence interval [CI], 0.81-2.30), length of stay (11.0 vs. 11.1 days; P=0.86), or charges ($123,557 vs. $124,425; P=0.79). Compared to the younger cohort, septuagenarians had higher rates of in-hospital mortality (4.30% vs. 1.67%; P<0.01; OR, 2.64; 95% CI, 1.67-4.16), length of stay (12.1 vs. 11.1 days; P<0.01), and charges ($139,200 vs. $124,425; P<0.01). In the multivariate analysis, septuagenarians had higher mortality (P=0.01; adjusted odds ratio [aOR], 2.01; 95% CI, 1.18-3.43). Similarly, compared to the younger cohort, octogenarians had a higher rate of mortality (7.67% vs. 1.67%; P<0.001; OR, 4.88; 95% CI, 3.06-7.79), length of stay (12.3 vs. 11.1 days; P<0.01), and charges ($131,330 vs. $124,425; P<0.01). In the multivariate analysis, octogenarians had higher mortality (P<0.001; aOR, 4.03; 95% CI, 2.28-7.11). Conclusions: Advanced age (>70 years) is an independent risk factor for postoperative death in patients with gastric cancer undergoing gastrectomy.

• Journal of Dental Anesthesia and Pain Medicine
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• 제22권3호
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• pp.217-226
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• 2022

Background: Intraosseous anesthesia is the process by which an anesthetic solution, after penetration of the cortical bone, is directly injected into the spongiosa of the alveolar bone supporting the tooth. This study aimed to compare the effectiveness of the traditional inferior alveolar nerve block (IANB) and computerized intraosseous anesthesia in the surgical extraction of impacted lower third molars, compare their side effects systemically by monitoring heart rate, and assess patients' a posteriori preference of one technique over the other. Methods: Thirty-nine patients with bilaterally impacted third molars participated in this study. Each patient in the sample was both a case and control, where the conventional technique was randomly assigned to one side (group 1) and the alternative method to the contralateral side (group 2). Results: The traditional technique was faster in execution than anesthesia delivered via electronic syringe, which took 3 min to be administered. However, it was necessary to wait for an average of 6 ± 4 min from the execution to achieve the onset of IANB, while the latency of intraosseous anesthesia was zero. Vincent's sign and lingual nerve anesthesia occurred in 100% of cases in group 1. In group 2, Vincent's sign was recorded in 13% of cases and lingual anesthesia in four cases. The average duration of the perceived anesthetic effect was 192 ± 68 min in group 1 and 127 ± 75 min in group 2 (P < 0.001). The difference between the heart rate of group 1 and group 2 was statistically significant. During infiltration in group 1, heartbeat frequency increased by 5 ± 13 beats per minute, while in group 2, it increased by 22 ± 10 beats per minute (P < 0.001). No postoperative complications were reported for either technique. Patients showed a preference of 67% for the alternative technique and 20% for the traditional, and 13% of patients were indifferent. Conclusion: The results identified intraosseous anesthesia as a valid alternative to conventional anesthesia in impacted lower third molar extraction.

• 정신신체의학
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• 제30권2호
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• pp.165-171
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• 2022

연구목적 본 연구는 후향적 의무기록 검토를 통해 조현병 환자에서 1년간의 2세대 항정신병약물 투여가 혈소판 활성에 미치는 영향을 알아보기 위해 시행되었다. 방 법 혈소판 활성의 지표로는 평균혈소판요소(mean platelet component, MPC) 값을 이용하였다. 첫 평균혈소판요소 측정 이후 1년간 지속적으로 2세대 항정신병약물을 꾸준히 복용한 것으로 확인된 조현병 환자 총 24명을 대상으로, 평균혈소판요소의 변화를 대응 표본 t 검정을 통해 검증하였다. 결 과 첫 측정 시 및 1년간의 2세대 항정신병약물 투여 후의 평균혈소판요소 값은 각각 26.5±1.4 g/dL와 25.6±1.8 g/dL로, 1년간의 약물 복용 후 유의하게 평균혈소판요소 값이 감소하였다. 결 론 본 연구는 1년간의 2세대 항정신병약물 투여 시 혈소판 활성이 증가될 수 있음을 시사하며, 임상 진료 시 이와 관련될 수 있는 심뇌혈관질환, 혈전색전성 질환 등의 위험성에 대해 면밀한 모니터링이 필요할 수 있음을 시사한다.

• 대한약침학회지
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• 제25권3호
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• pp.233-241
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• 2022

Objectives: Asthma is a chronic disease, and the demand for herbal medicines in this field has increased in recent years. The new findings highlight the role of the gut-lung axis in the pathophysiology of asthma. Hence, this study will evaluate the safety and efficacy of Glasthma syrup, an herbal formula based on Persian medicine, in improving asthma and regulating intestinal permeability. The formula consists of five herbal ingredients that have anti-inflammatory effects on the respiratory tract, also known as gut tonics. Methods: The study will be conducted as a placebo-controlled, triple-blind, randomized trial. It will consist of a 4-week intervention followed by a 4-week follow-up period. The target sample size is 20 patients with moderate asthma aged 18 to 60 years. Eligible participants will be randomly assigned to either the experimental group or the control group in equal numbers. Patients in the experimental group will take Glasthma syrup (7.5 mL, twice a day), while patients in the control group will take a matching placebo. Both groups will receive a 4-week combination of a long-acting beta2 agonist and a leukotriene modulator as standard of care. Inhaled corticosteroids can be used as rescue medication as needed. Results: The primary outcomes are asthma symptom scale, lung function, and intestinal permeability. Secondary outcomes include quality of life, symptom recurrence rates, and blood tests. A safety assessment will also be conducted during the trial. Conclusion: In this trial, the effects of Glasthma syrup in patients with moderate asthma will be examined. The study will also assess the effects of the formulation on the gut-lung axis by simultaneously monitoring the gut permeability index, asthma symptoms, and lung function.

• Imaging Science in Dentistry
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• 제53권3호
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• pp.193-198
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• 2023

Purpose: Artificial intelligence (AI) is poised to play a major role in medical diagnostics. Periodontal disease is one of the most common oral diseases. The early diagnosis of periodontal disease is essential for effective treatment and a favorable prognosis. This study aimed to assess the effectiveness of AI in diagnosing periodontal bone loss through radiographic analysis. Materials and Methods: A literature search involving 5 databases (PubMed, ScienceDirect, Scopus, Health and Medical Collection, Dentistry and Oral Sciences) was carried out. A specific combination of keywords was used to obtain the articles. The PRISMA guidelines were used to filter eligible articles. The study design, sample size, type of AI software, and the results of each eligible study were analyzed. The CASP diagnostic study checklist was used to evaluate the evidence strength score. Results: Seven articles were eligible for review according to the PRISMA guidelines. Out of the 7 eligible studies, 4 had strong CASP evidence strength scores (7-8/9). The remaining studies had intermediate CASP evidence strength scores (3.5-6.5/9). The highest area under the curve among the reported studies was 94%, the highest F1 score was 91%, and the highest specificity and sensitivity were 98.1% and 94%, respectively. Conclusion: AI-based detection of periodontal bone loss using radiographs is an efficient method. However, more clinical studies need to be conducted before this method is introduced into routine dental practice.

• 대한소아치과학회지
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• 제50권1호
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• pp.1-12
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• 2023

타액선의 기능과 타액의 구성성분은 개체의 건강 상태를 반영하는 지표가 될 수 있다. 타액에서 바이러스와 미생물, 호르몬, 면역 및 대사산물 등을 검출하는 미량원소 분석기술이 발달함에 따라, 전신건강의 진단, 평가, 예방 분야에서 타액의 활용가능성이 높아지고 있다. 진단 검체로써 타액은 혈액에 비해 채취 방법이 비 침습적이어서 환자의 불편감이 적고 비 전문가에 의한 검체 수집이 가능할 뿐 아니라 채취과정 중 감염 위험성이 낮다는 점에서 장점이 있다. 이러한 이유로 스트레스, 마이크로바이옴, 유전학 및 후생유전학 분야의 연구에 있어 타액 내 단백질, 유전물질이나 각종 생체표지자 등을 활용하는 방법이 주목받고 있다. 또한 전신 건강에 대한 빅데이터 수집 연구와 관련하여 타액을 효율적으로 활용, 보관하기 위한 인체 자원 은행의 필요성이 강조되고 있으며, 조직공학과 접목하여 타액선 재생연구도 활발히 진행되고 있다. 검체 채취법이나 보관, 활용 방법의 표준화를 비롯하여 해결해야 할 과제가 남아있으므로, 본 리뷰에서 타액 및 타액선에 관한 최근의 연구 동향을 알아보고 미래 발전 방향에 대하여 검토해 보고자 하였다.

• 동의신경정신과학회지
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• 제33권1호
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• pp.1-19
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• 2022

Objectives: The objective of this study was to confirm the Chiljeong (七情) characteristics of Hwa-byung patients using the core seven-emotions inventory-short form. Methods: We conducted a retrospective observational study based on the electronic medical records from a Korean medicine hospital. We included patients who were diagnosed with Hwa-byung and examined with Core Seven-Emotions Inventory-short Form (CSEI-S) during hospitalization periods. We presented the characteristics of Hwa-byung by demographic information, CSEI-S, Hwa-byung scale, and Korean Symptom Checklist 95. A correlation analysis was performed between CSEI-S and other clinical and psychological characteristics. Results: The Chiljeong characteristics of Hwa-byung were high in the order of Sorrow (悲), Thought (思), Fear (恐), Fright (驚), Depression (憂), Joy (喜), and Anger (怒). There was no significant difference between each emotion. After combined Korean medical treatment, Sorrow (悲) and Thought (思) significantly decreased. There were static correlations between sorrow (悲) and fright (驚), thought (思) and sorrow (悲), depression (憂) and sorrow (悲), depression (憂) and fright (驚), thought (思) and depression (憂), fear (恐) and fright (驚), anger (怒) and thought (思), thought (思) and fright (驚), sorrow (悲) and fear (恐). Sorrow (悲) and Hwa-byung characteristics scale showed static correlation. Joy (喜) showed a static correlation with disharmony between the heart and kidney scores of the Hwa-byung pattern identification. Between KSCL-95 and CSEI-S, static correlation appeared in depression (憂) with depression, anxiety, and sleep problem scale, sorrow (悲) with depression and anxiety, fright (驚) with depression and obsessive symptoms. Conclusions: Despite several limitations due to the study design and small sample size, this research successfully used CSEI-S to study the Chiljeong (七情) characteristics of Hwa-byung for the first time.

• Korean Journal of Radiology
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• 제22권8호
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• pp.1240-1252
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• 2021

Objective: To compare the accuracy for detecting breast cancer in the diagnostic setting between the use of digital breast tomosynthesis (DBT), defined as DBT alone or combined DBT and digital mammography (DM), and the use of DM alone through a systematic review and meta-analysis. Materials and Methods: Ovid-MEDLINE, Ovid-Embase, Cochrane Library and five Korean local databases were searched for articles published until March 25, 2020. We selected studies that reported diagnostic accuracy in women who were recalled after screening or symptomatic. Study quality was assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. A bivariate random effects model was used to estimate pooled sensitivity and specificity. We compared the diagnostic accuracy between DBT and DM alone using meta-regression and subgroup analyses by modality of intervention, country, existence of calcifications, breast density, Breast Imaging Reporting and Data System category threshold, study design, protocol for participant sampling, sample size, reason for diagnostic examination, and number of readers who interpreted the studies. Results: Twenty studies (n = 44513) that compared DBT and DM alone were included. The pooled sensitivity and specificity were 0.90 (95% confidence interval [CI] 0.86-0.93) and 0.90 (95% CI 0.84-0.94), respectively, for DBT, which were higher than 0.76 (95% CI 0.68-0.83) and 0.83 (95% CI 0.73-0.89), respectively, for DM alone (p < 0.001). The area under the summary receiver operating characteristics curve was 0.95 (95% CI 0.93-0.97) for DBT and 0.86 (95% CI 0.82-0.88) for DM alone. The higher sensitivity and specificity of DBT than DM alone were consistently noted in most subgroup and meta-regression analyses. Conclusion: Use of DBT was more accurate than DM alone for the diagnosis of breast cancer. Women with clinical symptoms or abnormal screening findings could be more effectively evaluated for breast cancer using DBT, which has a superior diagnostic performance compared to DM alone.

• Korean Circulation Journal
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• 제53권12호
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• pp.829-839
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• 2023

Background and Objectives: There is limited data on the impact of type 2 myocardial infarction (T2MI) during the coronavirus disease 2019 (COVID-19) pandemic. Methods: The National Inpatient Sample (NIS) database from January 2019 to December 2020 was queried to identify T2MI hospitalizations based on the appropriate International Classification of Disease, Tenth Revision-Clinical Modification codes. Monthly trends of COVID-19 and T2MI hospitalizations were evaluated using Joinpoint regression analysis. In addition, the multivariate logistic and linear regression analysis was used to compare inhospital mortality, coronary angiography use, and resource utilization between 2019 and 2020. Results: A total of 743,535 patients hospitalized with a diagnosis of T2MI were identified in the years 2019 (n=331,180) and 2020 (n=412,355). There was an increasing trend in T2MI hospitalizations throughout the study period corresponding to the increase in COVID-19 hospitalizations in 2020. The adjusted odds of in-hospital mortality associated with T2MI hospitalizations were significantly higher in 2020 compared with 2019 (11.1% vs. 8.1%: adjusted odds ratio, 1.19 [1.13-1.26]; p<0.01). In addition, T2MI hospitalizations were associated with lower odds of coronary angiography and higher total hospitalization charges, with no difference in the length of stay in 2020 compared with 2019. Conclusions: We found a significant increase in T2MI hospitalizations with higher in-hospital mortality, total hospitalization costs, and lower coronary angiography use during the early COVID-19 pandemic corresponding to the trends in the rise of COVID-19 hospitalizations. Further research into the factors associated with increased mortality can increase our preparedness for future pandemics.